伽蓝菜属植物遗传转化及其应用研究进展
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摘要
伽蓝菜属(Kalanchoe)植物在园艺观赏和传统医药领域具有重要的应用价值,而且为基础理论的研究,特别是在景天科代谢途径和体细胞胚发生的分子机制探讨方面,提供了非常理想的模式系统。遗传转化方法是关系到植物性状定向改良和基因功能研究的核心技术,能极大加速育种进程和机制挖掘。本综述系统讲述了多种伽蓝菜属植物(K. laciniata, K. blossfeldiana, K daigremontiana, K.×houghtonii, K. pinnata, K.fedtschenkoi)的遗传转化方法,并且探讨了这些方法在改造花色、叶型、株型、花期、抗衰老等应用方面以及在景天科代谢途径和细胞命运转变的机理方面的研究进展,以期为进一步开发和利用伽蓝菜属植物提供比较全面的信息资源。
Kalanchoe plants play important roles in the fields of ornamental horticulture and traditional medicine,which also provide a very ideal model system for basic theory research, especially on the molecular mechanisms of CAM pathway and somatic embryogenesis. Genetic transformation is the core technology related to directional improvement of plant traits and investigation of gene functions, therefore leading to acceleration of breeding progress and mechanism exploration. This study systematically reviewed the transformation methods of multiple Kalanchoe plants(K. laciniata, K. blossfeldiana, K. daigremontiana, K.×houghtonii, K. pinnata, K. fedtschenkoi)and discussed the research progress of these methods on the application of genetic modification of flower color,leaf morphology, plant architecture, flowering time, anti-aging properties, and on the mechanism of CAM pathway and cell fate transition, so as to provide comprehensive information resources for further exploitation and utilization of Kalanchoe plants.
Kalanchoe plants play important roles in the fields of ornamental horticulture and traditional medicine,which also provide a very ideal model system for basic theory research, especially on the molecular mechanisms of CAM pathway and somatic embryogenesis. Genetic transformation is the core technology related to directional improvement of plant traits and investigation of gene functions, therefore leading to acceleration of breeding progress and mechanism exploration. This study systematically reviewed the transformation methods of multiple Kalanchoe plants(K. laciniata, K. blossfeldiana, K. daigremontiana, K.×houghtonii, K. pinnata, K. fedtschenkoi)and discussed the research progress of these methods on the application of genetic modification of flower color,leaf morphology, plant architecture, flowering time, anti-aging properties, and on the mechanism of CAM pathway and cell fate transition, so as to provide comprehensive information resources for further exploitation and utilization of Kalanchoe plants.
引文
Aida R.,and Shibata M.,1996,Tr ansformation of Kalanchoe blossfeldiana mediated by Agrobacterium tumefaciens and transgene silencing,Plant Sci.,121(2):175-185
Akinsulire O.R.,Aibinu I.E.,Adenipekum T.,Adelowotan T.,and Odugbemi T.,2007,In vitro antimicrobial activity of crude extracts from plants Bryophyllum pinnatum and Kalanchoe crenata,Afr.J.Trad.Complement Altern.Med.,4(3):338-344
Chernetskyy M.,2011,Problems in nomenclature and systematics in the subfamily kalanchoideae(Crassulaceae)over theyears,Acta Agrobotanica.,64(4):67-74
Christensen B.,Sriskandarajah S.,Serek M.,and Müller R.,2008,Transformation of Kalanchoe blossfeldiana with rolgenes is useful in molecular breeding towards compact growth,Plant Cell Rep.,27(9):1485-1495
Descoings B.,eds.,2003,Illustrated handbook of succulent plants,Springer Verlag,Berlin,Germany,pp.143-180
Dever L.V.,Boxall S.F.,KnerováJ.,and Hartwell J.,2015,Transgenic perturbation of the decarboxylation phase of Crassulacean acid metabolism alters physiology and metabolism but has only a small effect on growth,Plant Physiol.,167(1):44-59
Garcês H.,and Sinha N.,2009,Transformation of the plant Kalanchoe daigremontiana using Agrobacterium tumefaciens,Cold Spring Harb.Protoc.,(10):5303
Garcês H.M.,Champagne C.E.,Townsley B.T.,Park S.,MalhóR.,Pedroso M.C.,Harada J.J.,and Sinha N.R.,2007,Evolu tion of asexual reproduction in leaves of the genus Kalancho觕,Proc.Natl.Acad.Sci.USA,104(39):15578-15583
Garcês H.M.,Koenig D.,Townsley B.T.,Kim M.,and Sinha N.R.,2014,Truncation of LEAFY COTYLEDON1 protein is required for asexual reproduction in Kalancho觕daigremontiana,Plant Physiol.,165(1):196-206
Garcia-Sogo B.,Pineda B.,Castelblanque L.,Antón T.,Medina M.,Roque E.,Torresi C.,Beltrán J.P.,Moreno V.,and Ca觡as L.A.,2010,Efficient transformation of Kalanchoe blossfeldiana and production of male-sterile plants by engineered anther ablation,Plant Cell Rep.,29(1):61-77
Gargul J.M.,Mibus H.,and Serek M.,2013,Constitutive overexpression of Nicotiana GA2ox leads to compact phenotypes and delayed flowering in Kalancho觕blossfeldiana and Petunia hybrid,Plant Cell Tiss.Org.,115(3):407-418
Gargul J.M.,Mibus H.,and Serek M.,2015,Manipulation of MKS1 gene expression affects Kalanchoe blossfeldiana and Petunia hybrida phenotypes,Plant Biotechnol.J.,13(1):51-61
Giovannini A.,Zottini M.,Monreale G.,Spena A.,and Allavena A.,1999,Ornamental traits modification by rol genes in Osteospermum ecklonis transformed with Agrobacterium tumefaciens,In Vitro Cell Dev-Pl.,35(1):70-75
Ilczuk A.,Mibus H.,Schmidt M.,and Serek M.,2009,Use of the PMI/mannose selection system for genetic transformation of Kalanchoe blossfeldiana Poelln,Propag.Ornam.Plants,121(4):175-185
Jia S.R.,Yang M.Z.,Ott R.,and Chua N.H.,1989,High frequency transformation of Kalanchoe laciniata,Plant Cell Rep.,8(6):336-340
Jung Y.,Rhee Y.,Auh C.K.,Shim H.,Choi J.J.,Kwon S.T.,Yang J.S.,Kim D.,Kwon M.H.,Kim Y.S.,and Lee S.,2009,Production of recombinant single chain antibodies(scFv)in vegetatively reproductive Kalanchoe pinnata by in planta transformation,Plant Cell Rep.,28(10):1593-1602
Kuligowska K.,Lütken H.,Christensen B.,Skovgaard I.,Linde M.,Winkelmann T.,and Müller R.,2015,Evaluation of reproductive barriers contributes to the development of novel interspecific hybrids in the Kalancho觕genus,BMC Plant Biol.,15(1):15
Lans C.A.,2006,Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus,J.Ethnobiol.Ethnomed.,2(1):1-11
Laura M.,Borghi C.,Regis C.,Casetti A.,and Allavena A.,2009,Over-expression and silencing of KxhKN5 gene in K×houghtonii,Acta Hort.,836(836):265-269
Laura M.,Borghi C.,Regis C.,Cassetti A.,and Allavena A.,2013,Ectopic expression of Kxhkn5 in the viviparous species Kalanchoe×Houghtonii induces a novel pattern of epiphyll development,Transgenic Res.,22(1):59-74
Lutken H.,Jensen E.B.,Wallstr觟m S.V.,Muller R.,and Christensen B.,2012,Phenotypical and genotypical characterisation of compact Kalancho觕lines produced by a non-gmo transformation strategy,Acta Hortic.,938(938):503-508
Lutken H.,Jensen L.S.,Topp S.H.,Mibus H.,Muller R.,and Rasmussen S.K.,2010,Production of compact plants by overexpression of AtSHI in the ornamental Kalanchoe,Plant Biotech.J.,8(2):211-222
Lutken H.,Laura M.,Borghi C.,覫rgaard M.,Allavena A.,and Rasmussen S.K.,2011,Expression of KxhKN4 and KxhKN5genes in Kalancho觕blossfeldiana‘molly’results in novel compact plant phenotypes:towards a cisgenesis alternative to growth retardants,Plant Cell Rep.,30(12):2267-2279
Phopgao B.,Ruttaporn C.,and Warut U.K.,2015,A protocol for Agrobacterium-mediated transformation of Kalancho blossfeldiana with a flavonoid 3',5'hydroxylase(F3'5'H)gene,Afr.J.Biotechnol.,14(39):2765-2769
Sanikhani M.,Frello S.,and Serek M.,2006,TDZ induces shoot regeneration in various Kalancho觕blossfeldiana Poelln.cultivars in the absence of auxin,Plant Cell Tiss.Org.,85(1):75-82
Sanikhani M.,Mibus H.,Stummann B.M.,and Serek M.,2008,Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1-1 allele show reduced ethylene sensitivity,Plant Cell Rep.,27(4):729-737
Sazhina N.N.,Lapshin P.V.,Zagoskina N.V.,Korotkova E.I.,and Misin V.M.,2014,A comparative analysis of the antioxidant activity of Kalanchoe juices,Russ.J.Bioorg.Chem.,40(7):771-776
Shevchuk T.V.,Zakharchenko N.S.,Tarlachkov S.V.,Furs O.V.,Dyachenko O.V.,and Buryanov Y.I.,2016,35S promoter methylation in kanamycin-resistant kalanchoe(Kalanchoe pinnata L.)plants expressing the antimicrobial peptide cecropin P1 transgene,Biochemistry(Mosc.),81(9):968-971
Slightom J.L.,Durand-Tardif M.,Jouanin L.,and Tepfer D.,1986,Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid.Identification of open reading frames,J.Biol.Chem.,261(1):108-121
Supratman U.,Fujita T.,Akiyama K.,and Hayashi H.,2000,New insecticidal bufadienolide,bryophyllin C,from Kalanchoe pinnata,Biosci.Biotechnol.Biochem.,64(6):1310-1312
Supratman U.,Fujita T.,Akiyama K.,and Hayashi H.,2001a,Insecticidal compounds from Kalanchoe daigremontiana×tubiflora,Phytochem.,58(2):311-314
Supratman U.,Fujita T.,Akiyama K.,Hayashi H.,Murakami A.,Sakai H.,Koshimizu K.,and Ohigashi H.,2001b,Anti-tumor promoting activity of bufadienolides from Kalanchoe pinnata and K.daigremontiana×tubiflora,Biosci.Biotech.Bioch.,65(4):947-949
Thirukkumaran G.,Khan R.S.,Chin D.P.,Nakamura I.,and Mii M.,2009,Isopentenyl transferase gene expression offers the positive selection of marker-free transgenic plant of Kalanchoe blossfeldiana,Plant Cell Tiss.Org.Cult.,97(3):237-242
Thirukkumarana G.,Otang N.V.,Sher K.R.,Nakamura I.,and Mii M.,2010,Generation of phenotypically normal marker free transgenic plants of Kalanchoe blossfeldiana through hairy root induction,Plant Biotechnol.,27(2):147-153
Topp S.H.,Rasmussen S.K.,and Sander L.,2008,Alcohol induced silencing of gibberellin 20-oxidases in Kalanchoe blossfeldiana,Plant Cell Tissue Organ Cult.,93(3):241-248
Truesdale M.R.,Toldi O.,and Scott P.,1999,The effect of elevated concentrations of fructose 2,6-bisphosphate on carbon metabolism during deacidification in the crassulacean acid metabolism plant Kalanch觟e daigremontiana,Plant Physiol.,121(3):957-964
Zakharchenko N.S.,Rukavtsova E.B.,Shevchuk T.V.,Furs O.V.,Pigoleva S.V.,Lebedeva A.A.,Chulina I.A.,Baidakova L.K.,and Bur'yanov Y.I.,2016,The obtainment and characteristics of Kalanchoe pinnata L.plants expressing the artificial gene of the cecropin P1 antimicrobial peptide,Appl.Biochem.Microbiol.,52(4):421-428
Akinsulire O.R.,Aibinu I.E.,Adenipekum T.,Adelowotan T.,and Odugbemi T.,2007,In vitro antimicrobial activity of crude extracts from plants Bryophyllum pinnatum and Kalanchoe crenata,Afr.J.Trad.Complement Altern.Med.,4(3):338-344
Chernetskyy M.,2011,Problems in nomenclature and systematics in the subfamily kalanchoideae(Crassulaceae)over theyears,Acta Agrobotanica.,64(4):67-74
Christensen B.,Sriskandarajah S.,Serek M.,and Müller R.,2008,Transformation of Kalanchoe blossfeldiana with rolgenes is useful in molecular breeding towards compact growth,Plant Cell Rep.,27(9):1485-1495
Descoings B.,eds.,2003,Illustrated handbook of succulent plants,Springer Verlag,Berlin,Germany,pp.143-180
Dever L.V.,Boxall S.F.,KnerováJ.,and Hartwell J.,2015,Transgenic perturbation of the decarboxylation phase of Crassulacean acid metabolism alters physiology and metabolism but has only a small effect on growth,Plant Physiol.,167(1):44-59
Garcês H.,and Sinha N.,2009,Transformation of the plant Kalanchoe daigremontiana using Agrobacterium tumefaciens,Cold Spring Harb.Protoc.,(10):5303
Garcês H.M.,Champagne C.E.,Townsley B.T.,Park S.,MalhóR.,Pedroso M.C.,Harada J.J.,and Sinha N.R.,2007,Evolu tion of asexual reproduction in leaves of the genus Kalancho觕,Proc.Natl.Acad.Sci.USA,104(39):15578-15583
Garcês H.M.,Koenig D.,Townsley B.T.,Kim M.,and Sinha N.R.,2014,Truncation of LEAFY COTYLEDON1 protein is required for asexual reproduction in Kalancho觕daigremontiana,Plant Physiol.,165(1):196-206
Garcia-Sogo B.,Pineda B.,Castelblanque L.,Antón T.,Medina M.,Roque E.,Torresi C.,Beltrán J.P.,Moreno V.,and Ca觡as L.A.,2010,Efficient transformation of Kalanchoe blossfeldiana and production of male-sterile plants by engineered anther ablation,Plant Cell Rep.,29(1):61-77
Gargul J.M.,Mibus H.,and Serek M.,2013,Constitutive overexpression of Nicotiana GA2ox leads to compact phenotypes and delayed flowering in Kalancho觕blossfeldiana and Petunia hybrid,Plant Cell Tiss.Org.,115(3):407-418
Gargul J.M.,Mibus H.,and Serek M.,2015,Manipulation of MKS1 gene expression affects Kalanchoe blossfeldiana and Petunia hybrida phenotypes,Plant Biotechnol.J.,13(1):51-61
Giovannini A.,Zottini M.,Monreale G.,Spena A.,and Allavena A.,1999,Ornamental traits modification by rol genes in Osteospermum ecklonis transformed with Agrobacterium tumefaciens,In Vitro Cell Dev-Pl.,35(1):70-75
Ilczuk A.,Mibus H.,Schmidt M.,and Serek M.,2009,Use of the PMI/mannose selection system for genetic transformation of Kalanchoe blossfeldiana Poelln,Propag.Ornam.Plants,121(4):175-185
Jia S.R.,Yang M.Z.,Ott R.,and Chua N.H.,1989,High frequency transformation of Kalanchoe laciniata,Plant Cell Rep.,8(6):336-340
Jung Y.,Rhee Y.,Auh C.K.,Shim H.,Choi J.J.,Kwon S.T.,Yang J.S.,Kim D.,Kwon M.H.,Kim Y.S.,and Lee S.,2009,Production of recombinant single chain antibodies(scFv)in vegetatively reproductive Kalanchoe pinnata by in planta transformation,Plant Cell Rep.,28(10):1593-1602
Kuligowska K.,Lütken H.,Christensen B.,Skovgaard I.,Linde M.,Winkelmann T.,and Müller R.,2015,Evaluation of reproductive barriers contributes to the development of novel interspecific hybrids in the Kalancho觕genus,BMC Plant Biol.,15(1):15
Lans C.A.,2006,Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus,J.Ethnobiol.Ethnomed.,2(1):1-11
Laura M.,Borghi C.,Regis C.,Casetti A.,and Allavena A.,2009,Over-expression and silencing of KxhKN5 gene in K×houghtonii,Acta Hort.,836(836):265-269
Laura M.,Borghi C.,Regis C.,Cassetti A.,and Allavena A.,2013,Ectopic expression of Kxhkn5 in the viviparous species Kalanchoe×Houghtonii induces a novel pattern of epiphyll development,Transgenic Res.,22(1):59-74
Lutken H.,Jensen E.B.,Wallstr觟m S.V.,Muller R.,and Christensen B.,2012,Phenotypical and genotypical characterisation of compact Kalancho觕lines produced by a non-gmo transformation strategy,Acta Hortic.,938(938):503-508
Lutken H.,Jensen L.S.,Topp S.H.,Mibus H.,Muller R.,and Rasmussen S.K.,2010,Production of compact plants by overexpression of AtSHI in the ornamental Kalanchoe,Plant Biotech.J.,8(2):211-222
Lutken H.,Laura M.,Borghi C.,覫rgaard M.,Allavena A.,and Rasmussen S.K.,2011,Expression of KxhKN4 and KxhKN5genes in Kalancho觕blossfeldiana‘molly’results in novel compact plant phenotypes:towards a cisgenesis alternative to growth retardants,Plant Cell Rep.,30(12):2267-2279
Phopgao B.,Ruttaporn C.,and Warut U.K.,2015,A protocol for Agrobacterium-mediated transformation of Kalancho blossfeldiana with a flavonoid 3',5'hydroxylase(F3'5'H)gene,Afr.J.Biotechnol.,14(39):2765-2769
Sanikhani M.,Frello S.,and Serek M.,2006,TDZ induces shoot regeneration in various Kalancho觕blossfeldiana Poelln.cultivars in the absence of auxin,Plant Cell Tiss.Org.,85(1):75-82
Sanikhani M.,Mibus H.,Stummann B.M.,and Serek M.,2008,Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1-1 allele show reduced ethylene sensitivity,Plant Cell Rep.,27(4):729-737
Sazhina N.N.,Lapshin P.V.,Zagoskina N.V.,Korotkova E.I.,and Misin V.M.,2014,A comparative analysis of the antioxidant activity of Kalanchoe juices,Russ.J.Bioorg.Chem.,40(7):771-776
Shevchuk T.V.,Zakharchenko N.S.,Tarlachkov S.V.,Furs O.V.,Dyachenko O.V.,and Buryanov Y.I.,2016,35S promoter methylation in kanamycin-resistant kalanchoe(Kalanchoe pinnata L.)plants expressing the antimicrobial peptide cecropin P1 transgene,Biochemistry(Mosc.),81(9):968-971
Slightom J.L.,Durand-Tardif M.,Jouanin L.,and Tepfer D.,1986,Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid.Identification of open reading frames,J.Biol.Chem.,261(1):108-121
Supratman U.,Fujita T.,Akiyama K.,and Hayashi H.,2000,New insecticidal bufadienolide,bryophyllin C,from Kalanchoe pinnata,Biosci.Biotechnol.Biochem.,64(6):1310-1312
Supratman U.,Fujita T.,Akiyama K.,and Hayashi H.,2001a,Insecticidal compounds from Kalanchoe daigremontiana×tubiflora,Phytochem.,58(2):311-314
Supratman U.,Fujita T.,Akiyama K.,Hayashi H.,Murakami A.,Sakai H.,Koshimizu K.,and Ohigashi H.,2001b,Anti-tumor promoting activity of bufadienolides from Kalanchoe pinnata and K.daigremontiana×tubiflora,Biosci.Biotech.Bioch.,65(4):947-949
Thirukkumaran G.,Khan R.S.,Chin D.P.,Nakamura I.,and Mii M.,2009,Isopentenyl transferase gene expression offers the positive selection of marker-free transgenic plant of Kalanchoe blossfeldiana,Plant Cell Tiss.Org.Cult.,97(3):237-242
Thirukkumarana G.,Otang N.V.,Sher K.R.,Nakamura I.,and Mii M.,2010,Generation of phenotypically normal marker free transgenic plants of Kalanchoe blossfeldiana through hairy root induction,Plant Biotechnol.,27(2):147-153
Topp S.H.,Rasmussen S.K.,and Sander L.,2008,Alcohol induced silencing of gibberellin 20-oxidases in Kalanchoe blossfeldiana,Plant Cell Tissue Organ Cult.,93(3):241-248
Truesdale M.R.,Toldi O.,and Scott P.,1999,The effect of elevated concentrations of fructose 2,6-bisphosphate on carbon metabolism during deacidification in the crassulacean acid metabolism plant Kalanch觟e daigremontiana,Plant Physiol.,121(3):957-964
Zakharchenko N.S.,Rukavtsova E.B.,Shevchuk T.V.,Furs O.V.,Pigoleva S.V.,Lebedeva A.A.,Chulina I.A.,Baidakova L.K.,and Bur'yanov Y.I.,2016,The obtainment and characteristics of Kalanchoe pinnata L.plants expressing the artificial gene of the cecropin P1 antimicrobial peptide,Appl.Biochem.Microbiol.,52(4):421-428