甲基丙二酸质控滤纸片的配制及其气相色谱-质谱联用技术检测尿液有机酸的应用评估
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摘要
目的探讨甲基丙二酸质控滤纸片的配制方法及其在气相色谱-质谱联用技术测定尿有机酸方法中的应用价值。方法收集健康者尿液标本,根据肌酐值及目标浓度添加甲基丙二酸溶液,配成甲基丙二酸尿液。采用4cm×6cm专用滤纸片浸取甲基丙二酸尿液后自然晾干,制成甲基丙二酸质控滤纸片。根据CNAS-GL03要求,对质控滤纸片的均匀性、稳定性进行评价。结果甲基丙二酸质控滤纸片的最终浓度达到目标浓度,均匀性、稳定性评估结果表明该质控品基质均匀、稳定期至少1年。结论本研究成功配制了用于气相色谱-质谱联用技术检测有机酸的甲基丙二酸质控滤纸片,为研制含其他有机酸的复合项目室内质控品提供了思路。
Objective To explore the method of preparing methylmalonic acid control based filter paper in laboratoty and its value in gas chromatography-mass spectrometry for the determination of urinary organic acid.Methods Normal urine samples were collected,and methylmalonic acid solution was added according to the creatinine value and the target concentration,dubbed methylmalonic acid urine.With 4 cm ×6 cm special filter paper leaching methylmalonic acid urine and then dried to produce methylmalonic acid quality control filter paper,uniformity and stabilityand was evaluated according to CNAS-GL03.Results The final concentration of methylmalonic acid control filter paper reached the target concentration,and the uniformity and stability results showed that the quality control matrix was uniform and stable for at least one year.Conclusion The methylmalonic acid control filter paper has been successfully prepared for the detection of organic acids by gas chromatography-mass spectrometry.It fills the blank of the test system without indoor quality assessment,and provides ideas for the development of composite project with other organic acids.
Objective To explore the method of preparing methylmalonic acid control based filter paper in laboratoty and its value in gas chromatography-mass spectrometry for the determination of urinary organic acid.Methods Normal urine samples were collected,and methylmalonic acid solution was added according to the creatinine value and the target concentration,dubbed methylmalonic acid urine.With 4 cm ×6 cm special filter paper leaching methylmalonic acid urine and then dried to produce methylmalonic acid quality control filter paper,uniformity and stabilityand was evaluated according to CNAS-GL03.Results The final concentration of methylmalonic acid control filter paper reached the target concentration,and the uniformity and stability results showed that the quality control matrix was uniform and stable for at least one year.Conclusion The methylmalonic acid control filter paper has been successfully prepared for the detection of organic acids by gas chromatography-mass spectrometry.It fills the blank of the test system without indoor quality assessment,and provides ideas for the development of composite project with other organic acids.
引文
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[3]KIMURA M,YAMAMOTOT T,YAMAGUCHI S,et al.Automatic auromated metabolic profiling and interpretation of GCMS data for organic acidem ia screening apersonal computer based system[J].Tohoku J Exp Med,1999,18(8):317-334.
[4]中国合格评定国家认可委员会.能力验证样本均匀性和稳定性评价指南:CNAS-GL03[S].北京:中国合格评定国家认可委员会,2018.
[5]中国合格评定国家认可委员会.医学实验室质量和能力认可准则:CNAS-CL02[S].北京:中国合格评定国家认可委员会,2014.
[6]金献忠,郑曙昭,丘寅.能力验证样品均匀性和稳定性检验的统计方法[J].现代测量与实验室管理,2003,11(4):35-37.
[7]杨楠,韩连书,叶军,等.新生儿期氨基酸、有机酸及脂肪酸氧化代谢病疾病谱分析[J].临床儿科杂志,2012,30(9):805-808.