参苓白术散对CD大鼠血清及结肠组织中IL-1β、IL-4及IL-10含量的影响
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摘要
目的:通过观察参苓白术散对克罗恩氏病(CD)大鼠血清及组织中白介素1β、白细胞介素4(IL-4)及白细胞介素10(IL-10)含量的影响,探讨参苓白术散治疗克罗恩氏病的效应机制。方法:受试大鼠按随机数字表法分为空白组和模型组,采用三硝基苯磺酸(TNBS)合乙醇溶液灌肠制备克罗恩氏病大鼠模型。造模成功后将模型大鼠分为模型组、参苓白术散组和阳性对照组(美沙拉嗪组),空白组和模型组大鼠每日给予10ml/kg蒸馏水灌胃,参苓白术散组给予12g·kg-1·d-1参苓白术散药剂灌胃,阳性对照组给予0.2g·kg-1·d-1美沙拉嗪颗粒灌胃,持续治疗21d后,分别对各组大鼠血清及组织中IL-1β、IL-4和IL-10含量进行检测。结果:与空白组比较,模型组大鼠一般生存状况较差,血清及组织中IL-1β含量显著升高,IL-4和IL-10含量降低(P<0.05);与模型组比较,参苓白术散组大鼠一般生存状况明显改善,血清及结肠组织中IL-1β含量显著降低(P<0.05),而IL-4和IL-10含量升高(P<0.05)。结论:参苓白术散具有改善克罗恩氏病大鼠一般生存状况,调节结肠组织Th1/Th2细胞因子平衡分泌的作用。
Objective: To study effects of Shenling Baizhu San(SLBZ) on IL-1β, IL-4 and IL-10 contents in colon serum and tissue of rats with Crohn's disease. Methods: Rats were randomly divided into 2 groups: normal group and model group. After UC modle was successful, UC rats were numbered and grouped by random number table into the model group, SLBZ group and positive group; then rats in normal and model group were intragastrically administrated with distilled water 10 ml/kg every day, SLBZ group with SLBZ volume of 12g·kg-1·d-1, and positive group with suspension of mesalazine 0.2g·kg-1·d-1, for 21 consecutive days. Levels of IL-1β, IL-4, and IL-10 in colon serum and tissue of rats in each group were detected. Results: In successful model group, generally living conditions of rats were poor, levels of IL-1β in colon serum and tissue were much higher(P<0.01); but levels of IL-4 and IL-10 were lower than those in the normal group(P<0.05). Compared with the model group, the general survival of the state in the SLBZ San group was improved significantly. SLBZ could increase levels of IL-4 and IL-10 significantly(P<0.05), decrease levels of IL-1β(P<0.05). Conclusion: It was suggested that SLBZ has functions of improving general living conditions, adjusting cytokine balance of TH1/TH2.
Objective: To study effects of Shenling Baizhu San(SLBZ) on IL-1β, IL-4 and IL-10 contents in colon serum and tissue of rats with Crohn's disease. Methods: Rats were randomly divided into 2 groups: normal group and model group. After UC modle was successful, UC rats were numbered and grouped by random number table into the model group, SLBZ group and positive group; then rats in normal and model group were intragastrically administrated with distilled water 10 ml/kg every day, SLBZ group with SLBZ volume of 12g·kg-1·d-1, and positive group with suspension of mesalazine 0.2g·kg-1·d-1, for 21 consecutive days. Levels of IL-1β, IL-4, and IL-10 in colon serum and tissue of rats in each group were detected. Results: In successful model group, generally living conditions of rats were poor, levels of IL-1β in colon serum and tissue were much higher(P<0.01); but levels of IL-4 and IL-10 were lower than those in the normal group(P<0.05). Compared with the model group, the general survival of the state in the SLBZ San group was improved significantly. SLBZ could increase levels of IL-4 and IL-10 significantly(P<0.05), decrease levels of IL-1β(P<0.05). Conclusion: It was suggested that SLBZ has functions of improving general living conditions, adjusting cytokine balance of TH1/TH2.
引文
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[12]Wu K,Bi Y,Sun K,et al.IL-10-producing type 1 regulatory T cells and allergy[J].Cell Mol Immunol,2007,4(4):269-275.
[2]罗瑞.白术茯苓汤、香连丸及其合方调节克罗恩病大鼠细胞因子与肠黏膜修复的比较研究[D].成都:成都中医药大学,2011.
[3]Morris G P,Beck P L,Herridge M S,et al.Hapten-induced model of chronic inflammation and ulceration in the rat colon[J].Gastroenterology,1989,96(3):795-803.
[4]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993.
[5]张东坡.加减参苓白术散治疗慢性结肠炎65例临床观察[J].实用中西医结合临床,2007(4):48.
[6]李莉.参苓白术散治疗慢性腹泻38例[J].陕西中医学院学报,2010(3):28.
[7]张燕龙.参苓白术散加味治疗久泻40例[J].实用中医药杂志,2007(9):563.
[8]Crohn BB,Ginzburg L,Oppenheimer GD.Regional ileitis:a pathologic and clinical entity.1932[J].Mt Sinai J Med,2000,67(3):263-268.
[9]Berkes J,Viswanathan V K,Savkovic S D,et al.Intestinal epithelial responses to enteric pathogens:effects on the tight junction barrier,ion transport,and inflammation[J].Gut,2003,52(3):439-451.
[10]Ferretti M,Casini-Raggi V,Pizarro TT,et al.Neutralization of endogenous IL-1 receptor antagonist exacerbates and prolongs inflammation in rabbit immune colitis[J].J Clin Invest,1994,94(1):449-453.
[11]Iijima H,Takahashi I,Kishi D,et al.Alteration of interleukin 4production results in the inhibition of T helper type 2 cell-dominated inflammatory bowel disease in T cell receptor alpha chain-deficient mice[J].J Exp Med,1999,190(5):607-615.
[12]Wu K,Bi Y,Sun K,et al.IL-10-producing type 1 regulatory T cells and allergy[J].Cell Mol Immunol,2007,4(4):269-275.