肝郁脾虚证多发性抽动症大鼠模型的建立及评价
|
摘要
目的:建立肝郁脾虚证多发性抽动症大鼠动物模型,初步了解该模型的一般特征。方法:亚氨基二丙腈(IDPN)腹腔注射诱导法后,用饮食失节、慢性束缚以及过度疲劳法建立多发性抽动症肝郁脾虚证大鼠模型。通过自主活动计数、刻板运动评分、体重、食量、胸腺及脾脏指数、胃泌素、D-木糖等指标对肝郁脾虚证多发性抽动症大鼠进行评价。结果:模型组、肝郁脾虚组大鼠造模后在相同的时间内活动路程及平均速度较空白对照组明显增加。肝郁脾虚组大鼠的进食量减少,胸腺与脾脏指数,胃泌素及D-木糖浓度差异均有统计学意义(P <0. 05)。结论:本模型有儿童多发性抽动症的动作性抽动和发声性抽动的症状,同时具有小儿多发性抽动症中医证型中的肝郁脾虚证中情志抑郁,神疲气短,少气懒言,食少便溏等相似症状。动物模型的表面效度方面具有较大的相似程度。
Objective: To establish rats model of tourette' s syndrome( TS) in mouse with liver stagnation and spleen deficiency syndrome,and to explore the general characteristics of the model. Methods: After the induction of iminodipropionitrile( IDPN) by intraperitoneal injection,a rat model of multiple tic disorder with liver stagnation and spleen deficiency was established by eating dislocation,chronic restraint and excessive fatigue. The independent activity counts,stereotyped movement score,body weight,food intake,feces granu,thymus and spleen index,gastrin,D-xylose and other indexes were used to evaluate TS mouse of spleen deficiency. Results: In the model group and the liver stagnation and spleen deficiency group,the activity distance and the average speed of the rats in the same time were significantly increased compared with the blank control group. The food intake of rats in the liver stagnation and spleen deficiency group decreased,and the thymus index,spleen index,gastrin and D-xylose concentrations were statistically different( P < 0. 05). Conclusion: This model has relatively stable tic symptoms,and has some similar symptoms of liver stagnation and spleen deficiency syndrome,and to some extent has the surface validity of multiple tic disorder liver stagnation and spleen deficiency syndrome.
Objective: To establish rats model of tourette' s syndrome( TS) in mouse with liver stagnation and spleen deficiency syndrome,and to explore the general characteristics of the model. Methods: After the induction of iminodipropionitrile( IDPN) by intraperitoneal injection,a rat model of multiple tic disorder with liver stagnation and spleen deficiency was established by eating dislocation,chronic restraint and excessive fatigue. The independent activity counts,stereotyped movement score,body weight,food intake,feces granu,thymus and spleen index,gastrin,D-xylose and other indexes were used to evaluate TS mouse of spleen deficiency. Results: In the model group and the liver stagnation and spleen deficiency group,the activity distance and the average speed of the rats in the same time were significantly increased compared with the blank control group. The food intake of rats in the liver stagnation and spleen deficiency group decreased,and the thymus index,spleen index,gastrin and D-xylose concentrations were statistically different( P < 0. 05). Conclusion: This model has relatively stable tic symptoms,and has some similar symptoms of liver stagnation and spleen deficiency syndrome,and to some extent has the surface validity of multiple tic disorder liver stagnation and spleen deficiency syndrome.
引文
[1]刘智胜.小儿多发性抽动症[M].北京:人民卫生出版社,2002:32-65.
[2]Teive HA,Chien HF,Munhoz RP,et a1.Charcot'S contribution to the study of tourette'S syndrome[J].Arq Neuropsiquiatr,2008,66:918-921.
[3]Diamond BI,Reyes MG,Borison R.A new animal model for Tourette syndrome[J].Adv Neurol,1982,35(2):221-225.
[4]王道涵,王素梅,张雯,等.IDPN诱导TS模型小鼠纹状体EAAT2表达变化及健脾止动汤的干预研究[J].中华中医药杂志,2013,28(5):1471-1474.
[5]王道涵,王素梅,卫利,等.健脾止动汤对多发性抽动症模型小鼠纹状体多巴胺及其受体含量的影响[J].北京中医药大学学报,2012,35(7):452-455.
[6]刘晓芳,王素梅,卫利,等.健脾止动汤对Tourette综合征模型鼠纹状体多巴胺转运蛋白表达的影响[J].中华行为医学与脑科学杂志,2012,21(3):225-227.
[7]刘晓芳,王素梅,卫利,等.健脾止动汤对多发性抽动综合征模型鼠纹状体多巴胺D2受体mRNA的影响[J].中华行为医学与脑科学杂志,2013,22(4):296-298.
[8]梁媛,郭晓玲,岳广欣,等.逍遥散对肝郁脾虚证模型大鼠行为学变化的调节作用[J].北京中医药大学学报,2010,33(5):317-322.
[9]岳利峰,丁杰,陈家旭,等.肝郁脾虚证大鼠模型的建立与评价[J].北京中医药大学学报,2008,31(6)396-400.
[10]刘燕,邹小娟,丁秀芳.基于复合应激的肝郁脾虚证小鼠模型的建立与评价[J].中华中医药杂志,2016,5(21):80-84.
[11]盛昭园,王佑华,徐燕,等.健脾疏肝降脂汤对高脂血症大鼠血脂及炎症因子水平调节的作用[J].世界中西医结合杂志,2017,12(12):107-110.
[12]刘海洋,董文婷,雷霞,等.肝郁脾虚型慢性肝炎“病、证”复合模型的建立[J].中医药信息,2014,31(3):84-85.
[13]刘晓芳,卫利,张雯,等.王素梅教授从病因病机论小儿多发性抽动症的治疗[J].现代中医临床,2016,23(3):38-41.
[14]田佳鑫,马增春,王宇光,等.三种脾虚泄泻证模型大鼠消化系统功能改变的比较[J].中国临床康复,2006,10(39):129-131.
[15]蒋萃,李白雪,袁世清,等.脾虚证大鼠模型的制备方法研究进展及评价[J].环球中医药,2018,11(8):1333-1336.
[16]王洪海,谢鸣.复合病因造模法致脾虚证大鼠模型在免疫系统方面的变化[J].中国实验方剂学杂志,2006,12(12):41-45.
[17]陈琼科,王再谟.脾虚宁浸膏对脾虚泄泻动物模型红细胞免疫的影响[J].中国中西医结合消化杂志,2002,10(5):273-275.
[18]陈自佳,于文静,王素梅.脾虚证多发性抽动症小鼠模型的建立及评价[J].环球中医药,2016,9(9):1055-1058.
[19]张雯,于文静,王道涵,等.健脾止动汤对TS模型大鼠抗抽动效应及脑内兴奋性氨基酸递质的影响[J].中华中医药杂志,2014,29(5):1653-1656.
[20]ISOLA R,ZHANG H,TEJWANI GA,et al.Acute nicotine changes dynorphin and prodynorphin mRNA in the striatum[J].Psychopharmacology(Berl),2009,201(4):507-516.
[21]NYIANDER I,TERENIUS L.Chronic haloperidol and clozapine differentially affectdynorphin peptides and substance P in basal ganglia of the rat[J].Brain Res,1986,380(1):34-41.
[22]Herrer-Marschtiz M,Hokfelt T,Ungersted U,et al.Functional studies with the opioid peptide dynorphin:Acute effects of injections into the substantia nigra reticulata of naive rats[J].Life Sci,1983,33(1):555-558.
[2]Teive HA,Chien HF,Munhoz RP,et a1.Charcot'S contribution to the study of tourette'S syndrome[J].Arq Neuropsiquiatr,2008,66:918-921.
[3]Diamond BI,Reyes MG,Borison R.A new animal model for Tourette syndrome[J].Adv Neurol,1982,35(2):221-225.
[4]王道涵,王素梅,张雯,等.IDPN诱导TS模型小鼠纹状体EAAT2表达变化及健脾止动汤的干预研究[J].中华中医药杂志,2013,28(5):1471-1474.
[5]王道涵,王素梅,卫利,等.健脾止动汤对多发性抽动症模型小鼠纹状体多巴胺及其受体含量的影响[J].北京中医药大学学报,2012,35(7):452-455.
[6]刘晓芳,王素梅,卫利,等.健脾止动汤对Tourette综合征模型鼠纹状体多巴胺转运蛋白表达的影响[J].中华行为医学与脑科学杂志,2012,21(3):225-227.
[7]刘晓芳,王素梅,卫利,等.健脾止动汤对多发性抽动综合征模型鼠纹状体多巴胺D2受体mRNA的影响[J].中华行为医学与脑科学杂志,2013,22(4):296-298.
[8]梁媛,郭晓玲,岳广欣,等.逍遥散对肝郁脾虚证模型大鼠行为学变化的调节作用[J].北京中医药大学学报,2010,33(5):317-322.
[9]岳利峰,丁杰,陈家旭,等.肝郁脾虚证大鼠模型的建立与评价[J].北京中医药大学学报,2008,31(6)396-400.
[10]刘燕,邹小娟,丁秀芳.基于复合应激的肝郁脾虚证小鼠模型的建立与评价[J].中华中医药杂志,2016,5(21):80-84.
[11]盛昭园,王佑华,徐燕,等.健脾疏肝降脂汤对高脂血症大鼠血脂及炎症因子水平调节的作用[J].世界中西医结合杂志,2017,12(12):107-110.
[12]刘海洋,董文婷,雷霞,等.肝郁脾虚型慢性肝炎“病、证”复合模型的建立[J].中医药信息,2014,31(3):84-85.
[13]刘晓芳,卫利,张雯,等.王素梅教授从病因病机论小儿多发性抽动症的治疗[J].现代中医临床,2016,23(3):38-41.
[14]田佳鑫,马增春,王宇光,等.三种脾虚泄泻证模型大鼠消化系统功能改变的比较[J].中国临床康复,2006,10(39):129-131.
[15]蒋萃,李白雪,袁世清,等.脾虚证大鼠模型的制备方法研究进展及评价[J].环球中医药,2018,11(8):1333-1336.
[16]王洪海,谢鸣.复合病因造模法致脾虚证大鼠模型在免疫系统方面的变化[J].中国实验方剂学杂志,2006,12(12):41-45.
[17]陈琼科,王再谟.脾虚宁浸膏对脾虚泄泻动物模型红细胞免疫的影响[J].中国中西医结合消化杂志,2002,10(5):273-275.
[18]陈自佳,于文静,王素梅.脾虚证多发性抽动症小鼠模型的建立及评价[J].环球中医药,2016,9(9):1055-1058.
[19]张雯,于文静,王道涵,等.健脾止动汤对TS模型大鼠抗抽动效应及脑内兴奋性氨基酸递质的影响[J].中华中医药杂志,2014,29(5):1653-1656.
[20]ISOLA R,ZHANG H,TEJWANI GA,et al.Acute nicotine changes dynorphin and prodynorphin mRNA in the striatum[J].Psychopharmacology(Berl),2009,201(4):507-516.
[21]NYIANDER I,TERENIUS L.Chronic haloperidol and clozapine differentially affectdynorphin peptides and substance P in basal ganglia of the rat[J].Brain Res,1986,380(1):34-41.
[22]Herrer-Marschtiz M,Hokfelt T,Ungersted U,et al.Functional studies with the opioid peptide dynorphin:Acute effects of injections into the substantia nigra reticulata of naive rats[J].Life Sci,1983,33(1):555-558.