基于功能性分子标记鉴定香味杂交粳稻亲本
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摘要
香稻是由于水稻种水稻甜菜碱醛脱氢酶(BADH2)基因的功能缺失突变引起的。根据香味等位基因badh2的功能性突变位点设计的功能性分子标记,为快速鉴定具有香味的水稻种质资源提供了可能。本研究利用香味等位基因badh2-E2和badh2-E7的功能性分子标记InDel-E2和InDel-E7鉴定了33个杂交粳稻不育系和35个杂交粳稻恢复系材料的基因型,获得了12个含有badh2-E2等位基因不育系和4个含有badh2-E7等位基因恢复系材料。在此基础上,分别选择了3个不育系和3个恢复系,配制了5个全香型的杂交粳稻,为香味杂交粳稻的推广应用提供了理论依据。
Fragrant rice is caused by the functional loss and mutation of the Betaine aldehyde aehydrogenase(BADH2) gene.Functional molecular markers designed according to the functional mutation loci of the aroma allele badh2 make it possible for rapid identification the fragrant rice germplasm.In this study,33 male sterile lines and 35 restorer lines of japonica hybrid rice were genotyped by using functional markers InDel-E2 and InDel-E7,which were designed respectively for the fragrant badh2 alleles badh2-E2 and badh2-E7,12 sterile lines containing badh2-E2 allele and 4 restorer lines containing badh2-E7 allele were identified,respectively.On the basis of genotyping result,3 fragrant sterile lines and 3 fragrant restorer lines were selected to develop 5 fragrant japonica hybrid recombination.These results might provide an important basis for the popularization and application of fragrant japonica hybrid rice.
Fragrant rice is caused by the functional loss and mutation of the Betaine aldehyde aehydrogenase(BADH2) gene.Functional molecular markers designed according to the functional mutation loci of the aroma allele badh2 make it possible for rapid identification the fragrant rice germplasm.In this study,33 male sterile lines and 35 restorer lines of japonica hybrid rice were genotyped by using functional markers InDel-E2 and InDel-E7,which were designed respectively for the fragrant badh2 alleles badh2-E2 and badh2-E7,12 sterile lines containing badh2-E2 allele and 4 restorer lines containing badh2-E7 allele were identified,respectively.On the basis of genotyping result,3 fragrant sterile lines and 3 fragrant restorer lines were selected to develop 5 fragrant japonica hybrid recombination.These results might provide an important basis for the popularization and application of fragrant japonica hybrid rice.
引文
A marawathi Y.,Singh R.,Sing h A.K.,Singh V.P.,Mohapatra T.,Sharma T.R.,and Sin Sgh N.K.,2008,Mapping of quantitative trait loci for basmati quality traits in rice(Oryza sativa L.),Mol.Breeding,21(1):49-65
Bai D.L.,Wei W.,Wei Y.P.,Chen Y.Z.,and Li R.B.,2008,Status and prospect of aromatic hybrid rice,Guangxi Nongye Kexue(Guanxi Agricultural Sciences),39(6):717-724(白德朗,魏薇,韦燕萍,陈英之,李容柏,2008,杂交香稻研究现状与展望,广西农业科学,39(6):717-724)
Bradbury L.M.T.,Fitzgerald T.L.,Henry R.J.,Jin Q.S.,and Waters D.L.E.,2005,The gene for fragrance in rice,Plant Biotechnol.J.,3(3):363-370
Dhulappanavar C.V.,1976,Inheritance of scent in rice,Euphytica,25(1):659-662
He Q.,and Park Y.J.,2015,Discovery of a novel fragrant allele and development of functional markers for fragrance in rice,Mol.Breeding,35(11):217
Kovach M.J.,Calingacion M.N.,Fitzgerald M.A.,and Mccouch S.R.,2009,The origin and evolution of fragrance in rice(Oryza sativa L.),Proc.Natl.Acad.Sci.USA,106(34):14444-14449
Li Z.L.,Qiao Y.C.,Lin J.Y.,and Li G.G.,2015,Studies on molecular markers to Fusarium wilt resistance gene in Chiehqua,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(9):1946-1949(李兆龙,乔燕春,林锦英,李光光,2015,节瓜抗枯萎病基因的分子标记研究,基因组学与应用生物学,35(9):1946-1949)
Lu Y.T.,Liu Q.L.,Wang J.M.,Yan W.C.,Yu F.M.,and Jin Q.S.,2008,Detection of rice fragrant gene by allele-specific amplification,Zuowu Xuebao(Acta Agronomica Sinica),34(2):243-246(陆艳婷,刘庆龙,王俊敏,严文潮,俞法明,金庆生,2008,利用等位基因特异扩增快速检测水稻香味基因,作物学报,34(2):243-246)
Ootsuka K.,Takahashi I.,Tanaka K.,Itani T.,Tabuchi H.,Yoshihashi T.,Tonouchi A.,and Ishikawa R.,2014,Genetic polymorphisms in Japanese fragrant landraces and novel fragrant allele domesticated in Northern Japan,Breeding Sci.,64(2):115-124
Shao G.,Tang S.,Chen M.,Wei X.,He J.,Luo J.,Jiao G.,Hu Y.,Xie L.,and Hu P.,2013,Haplotype variation at Badh2,the gene determining fragrance in rice,Genomics,101(2):157-162
Shao G.N.,Tang A.,Tang S.Q.,Luo J.,Jiao G.A.,Wu J.L.,and Hu P.S.,2011,A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice,Plant Breeding,130(2):172-176
Shi W.W.,Yi Y.,Chen S.H.,and Xu M.L.,2008,Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties,Mol.Breeding,22(2):185-192
Shi Y.Q.,Zhao G.C.,Xu X.L.,and Li J.Y.,2014,Discovery of a new fragrance allele and development of functional markers for identifying diverse fragrant genotypes in rice,Mol.Breeding,33(3):701-708
Sood B.C.,and Siddiq E.A.,1975,A rapid technique for scent determination in rice,Indian J.Genet.Plant Breed.,38(2):268-275
Tang J.M.,Zhang S.S.,Cao Q.Y.,Guo Z.P.,Zhu C.Z.,Mao H.M.,Zhang C.Z.,Lei C.Y.,Fu C.J.,and Xu B.,2013,Breeding and application of aromatic medium indica hybrid rice combination Hongxiangyou 68 with fine grain quality,Zajiao Shuidao(Hybrid Rice),28(4):18-20(汤俭民,张似松,曹庆云,郭子平,朱彩章,毛惠民,张楚珍,雷昌云,付成俊,徐波,2013,全香型优质杂交香稻新组合红香优68的选育与应用,杂交水稻,28(4):18-20)
Wang J.,Yang J.,Chen Z.D.,and Zhong W.G.,2008,Development and application of fragrance gene markers in rice,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),6(6):1209-1212(王军,杨杰,陈志德,仲维功,2008,水稻香米基因标记的开发与应用,分子植物育种,6(6):1209-1212)
Xu X.L.,Zhao G.C.,and Li J.Y.,2011,Development of molecular markers used to identify two types of fragrant rice and analysis of mutation sites of BADH2 gene in 24 varieties of fragrant rice,Zhiwu Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),33(6):667-673(徐小龙,赵国超,李建粤,2011,24种香稻品种甜菜碱醛脱氢酶2基因突变位点的分析及分子标记开发,植物分类与资源学报,33(6):667-673)
Yan Y.,Zhu G.M.,Zhang L.X.,Wan C.Z.,Cao L.M.,Zhao Z.P.,and Wu S.J.,2015,Development of molecular markers for fragrant gene and its application,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),35(2):269-274(闫影,诸光明,张丽霞,万常照,曹黎明,赵志鹏,吴书俊,2015,水稻香味基因分子标记的开发及应用,西北植物学报,35(2):269-274)
Yuan L.P.,2014,Development of hybrid rice to ensure food security,Rice Science,21(1):1-2
Zhou K.L.,1995,Xiangyou63,a quasiaromatic hybrid rice with good quality,high yeild and good disease resistance,Zajiao Shuidao(Hybrid Rice),19(3):23-24(周坤炉,1995,优质、高产、抗病的香型杂交稻组合香优63,杂交水稻,19(3):23-24)
Bai D.L.,Wei W.,Wei Y.P.,Chen Y.Z.,and Li R.B.,2008,Status and prospect of aromatic hybrid rice,Guangxi Nongye Kexue(Guanxi Agricultural Sciences),39(6):717-724(白德朗,魏薇,韦燕萍,陈英之,李容柏,2008,杂交香稻研究现状与展望,广西农业科学,39(6):717-724)
Bradbury L.M.T.,Fitzgerald T.L.,Henry R.J.,Jin Q.S.,and Waters D.L.E.,2005,The gene for fragrance in rice,Plant Biotechnol.J.,3(3):363-370
Dhulappanavar C.V.,1976,Inheritance of scent in rice,Euphytica,25(1):659-662
He Q.,and Park Y.J.,2015,Discovery of a novel fragrant allele and development of functional markers for fragrance in rice,Mol.Breeding,35(11):217
Kovach M.J.,Calingacion M.N.,Fitzgerald M.A.,and Mccouch S.R.,2009,The origin and evolution of fragrance in rice(Oryza sativa L.),Proc.Natl.Acad.Sci.USA,106(34):14444-14449
Li Z.L.,Qiao Y.C.,Lin J.Y.,and Li G.G.,2015,Studies on molecular markers to Fusarium wilt resistance gene in Chiehqua,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(9):1946-1949(李兆龙,乔燕春,林锦英,李光光,2015,节瓜抗枯萎病基因的分子标记研究,基因组学与应用生物学,35(9):1946-1949)
Lu Y.T.,Liu Q.L.,Wang J.M.,Yan W.C.,Yu F.M.,and Jin Q.S.,2008,Detection of rice fragrant gene by allele-specific amplification,Zuowu Xuebao(Acta Agronomica Sinica),34(2):243-246(陆艳婷,刘庆龙,王俊敏,严文潮,俞法明,金庆生,2008,利用等位基因特异扩增快速检测水稻香味基因,作物学报,34(2):243-246)
Ootsuka K.,Takahashi I.,Tanaka K.,Itani T.,Tabuchi H.,Yoshihashi T.,Tonouchi A.,and Ishikawa R.,2014,Genetic polymorphisms in Japanese fragrant landraces and novel fragrant allele domesticated in Northern Japan,Breeding Sci.,64(2):115-124
Shao G.,Tang S.,Chen M.,Wei X.,He J.,Luo J.,Jiao G.,Hu Y.,Xie L.,and Hu P.,2013,Haplotype variation at Badh2,the gene determining fragrance in rice,Genomics,101(2):157-162
Shao G.N.,Tang A.,Tang S.Q.,Luo J.,Jiao G.A.,Wu J.L.,and Hu P.S.,2011,A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice,Plant Breeding,130(2):172-176
Shi W.W.,Yi Y.,Chen S.H.,and Xu M.L.,2008,Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties,Mol.Breeding,22(2):185-192
Shi Y.Q.,Zhao G.C.,Xu X.L.,and Li J.Y.,2014,Discovery of a new fragrance allele and development of functional markers for identifying diverse fragrant genotypes in rice,Mol.Breeding,33(3):701-708
Sood B.C.,and Siddiq E.A.,1975,A rapid technique for scent determination in rice,Indian J.Genet.Plant Breed.,38(2):268-275
Tang J.M.,Zhang S.S.,Cao Q.Y.,Guo Z.P.,Zhu C.Z.,Mao H.M.,Zhang C.Z.,Lei C.Y.,Fu C.J.,and Xu B.,2013,Breeding and application of aromatic medium indica hybrid rice combination Hongxiangyou 68 with fine grain quality,Zajiao Shuidao(Hybrid Rice),28(4):18-20(汤俭民,张似松,曹庆云,郭子平,朱彩章,毛惠民,张楚珍,雷昌云,付成俊,徐波,2013,全香型优质杂交香稻新组合红香优68的选育与应用,杂交水稻,28(4):18-20)
Wang J.,Yang J.,Chen Z.D.,and Zhong W.G.,2008,Development and application of fragrance gene markers in rice,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),6(6):1209-1212(王军,杨杰,陈志德,仲维功,2008,水稻香米基因标记的开发与应用,分子植物育种,6(6):1209-1212)
Xu X.L.,Zhao G.C.,and Li J.Y.,2011,Development of molecular markers used to identify two types of fragrant rice and analysis of mutation sites of BADH2 gene in 24 varieties of fragrant rice,Zhiwu Fenlei Yu Ziyuan Xuebao(Plant Diversity and Resources),33(6):667-673(徐小龙,赵国超,李建粤,2011,24种香稻品种甜菜碱醛脱氢酶2基因突变位点的分析及分子标记开发,植物分类与资源学报,33(6):667-673)
Yan Y.,Zhu G.M.,Zhang L.X.,Wan C.Z.,Cao L.M.,Zhao Z.P.,and Wu S.J.,2015,Development of molecular markers for fragrant gene and its application,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),35(2):269-274(闫影,诸光明,张丽霞,万常照,曹黎明,赵志鹏,吴书俊,2015,水稻香味基因分子标记的开发及应用,西北植物学报,35(2):269-274)
Yuan L.P.,2014,Development of hybrid rice to ensure food security,Rice Science,21(1):1-2
Zhou K.L.,1995,Xiangyou63,a quasiaromatic hybrid rice with good quality,high yeild and good disease resistance,Zajiao Shuidao(Hybrid Rice),19(3):23-24(周坤炉,1995,优质、高产、抗病的香型杂交稻组合香优63,杂交水稻,19(3):23-24)