日本茵芋雌雄株的组织培养
|
摘要
为营建日本茵芋雌雄株组培快繁技术体系,以雌株品种‘帕贝拉’和雄株品种‘鲁贝拉’嫩枝茎段为外植体,研究其诱导、增殖、生根培养的主要影响因素,筛选最佳培养基。结果表明:日本茵芋雄株的诱导率、增殖倍率及生根率均低于雌株,且差异均达极显著水平;在较佳诱导培养基3.0 g·L~(-1)花宝1号+1.5 mg·L~(-1)6-苄氨基腺嘌呤(6-BA)上,雌雄株诱导率分别为75.6%、34.7%;结合切除芽苗顶芽的方法,在培养基3.0 g·L~(-1)花宝1号+2.5 mg·L~(-1)6-BA+4.0 mg·L~(-1)赤霉素(GA3)上,继代培养50 d,雌雄株增殖倍率分别为4.25、3.22倍;在培养基1.5 g·L~(-1)花宝1号+0.5 mg·L~(-1)生根粉1号(ABT 1号)上,雌雄株生根率分别为73%、61%;雌雄株组培苗移栽在黄心土+椰糠(3∶1)基质中,移栽成活率分别为86%、83%。
To establish a tissue culture technique and rapid propagation of female and male clones of Skimmia japonica,the young stems of female‘Pabella'and male ‘Rubella'were used as the explants; key factors that influence the initiation,proliferation,and rooting of the shoots were studied,and optimal media were screened. The results showed that the initiation rate,proliferation rate,and rooting rate of the males were lower than those of the females,and significant differences were detected between the females and males. The best initiation medium was 3 g·L~(-1) Hyponex No.1+1.5 mg·L~(-1)6-benzylaminopurine( 6-BA),in which the initiation rate of the females and males was 75.6% and 34.7%,respectively. The best proliferation medium was 3 g·L~(-1) hyponex No.1+2.5 mg·L~(-1)6-BA+4.0 mg·L~(-1) gibberellin A3( GA3),in which the proliferation rate of the females and males was 4.25 and3.22,respectively,by removing the terminal bud of the shoot after 50 days of subculture. The rooting rate of the females and male was 73% and 61%,respectively,in 1.5 g ·L~(-1) hyponex No.1 + 0.5 mg ·L~(-1) root powder No.1( ABT No.1). The transplantation survival rate of the females and males was 86% and 83%,respectively,when a mixture of yellow soil ∶ vermiculite = 3 ∶ 1 was used as the transplantation soil.
To establish a tissue culture technique and rapid propagation of female and male clones of Skimmia japonica,the young stems of female‘Pabella'and male ‘Rubella'were used as the explants; key factors that influence the initiation,proliferation,and rooting of the shoots were studied,and optimal media were screened. The results showed that the initiation rate,proliferation rate,and rooting rate of the males were lower than those of the females,and significant differences were detected between the females and males. The best initiation medium was 3 g·L~(-1) Hyponex No.1+1.5 mg·L~(-1)6-benzylaminopurine( 6-BA),in which the initiation rate of the females and males was 75.6% and 34.7%,respectively. The best proliferation medium was 3 g·L~(-1) hyponex No.1+2.5 mg·L~(-1)6-BA+4.0 mg·L~(-1) gibberellin A3( GA3),in which the proliferation rate of the females and males was 4.25 and3.22,respectively,by removing the terminal bud of the shoot after 50 days of subculture. The rooting rate of the females and male was 73% and 61%,respectively,in 1.5 g ·L~(-1) hyponex No.1 + 0.5 mg ·L~(-1) root powder No.1( ABT No.1). The transplantation survival rate of the females and males was 86% and 83%,respectively,when a mixture of yellow soil ∶ vermiculite = 3 ∶ 1 was used as the transplantation soil.
引文
[1]SHATTOCK R C,ASKEW S J.Stem lesions,canker and dieback of Skimmia japonic[J].Annals of Botany,1980,45(5):535-540.
[2]FUKUDA T,NAIKI A,NAGAMASU H.Karyotypic analysis of Skimmia japonica(Rutaceae)and related species[J].Journal of Plant Research,2007,120(1):113-121.
[3]WHALLEY D N,LOACH K.Modification of episodic growth in Skimmia japonica‘Rubella’by gibberellic acid treatment[J].Scientia Horticulturae,1978,9(1):83-92.
[4]王沣.红星茵芋扦插繁殖技术[J].林业科技开发,2011,25(2):114-116.
[5]诸春雯,汤桂钧,钱仲秀,等.茵芋嫩枝扦插繁育技术[J].中国花卉园艺,2008(14):35-36.
[6]周芳勇,王春,罗蔓,等.茶梅和红星茵芋盆栽基质的筛选[J].林业科技开发,2007,21(4):70-72.
[7]郑天汉,李勇,吴远彬,等.茵芋驯化栽培与植物生长调节剂促进效应研究[J].天津农业科学,2016,22(8):125-129.
[8]江斌,张文融,陈碧华,等.红星茵芋组织培养技术研究[J].中国园艺文摘,2016,32(3):12-13,21.
[9]孟艳琼,李仁杰,伊兴凯,等.红星茵芋离体花梗腋芽诱导及丛生芽增殖研究[J].中国农学通报,2007,23(11):81-85.
[10]黄益基,吕秀立,付瑞,等.金红茵芋离体快速繁殖技术的研究[J].江苏林业科技,2017,44(4):8-12,17.
[11]PONCHIA G,ZANIN G.In vitro propagation of Skimmia(Skimmia japonica Thunb.)[J].Acta Horticulturae,2000,520(520):91-96.
[12]闫国华,周宇,张晓明,等.植物离体培养中的顽拗现象及其生理和遗传基础[J].植物生理学通讯,2002,38(5):481-486.
[2]FUKUDA T,NAIKI A,NAGAMASU H.Karyotypic analysis of Skimmia japonica(Rutaceae)and related species[J].Journal of Plant Research,2007,120(1):113-121.
[3]WHALLEY D N,LOACH K.Modification of episodic growth in Skimmia japonica‘Rubella’by gibberellic acid treatment[J].Scientia Horticulturae,1978,9(1):83-92.
[4]王沣.红星茵芋扦插繁殖技术[J].林业科技开发,2011,25(2):114-116.
[5]诸春雯,汤桂钧,钱仲秀,等.茵芋嫩枝扦插繁育技术[J].中国花卉园艺,2008(14):35-36.
[6]周芳勇,王春,罗蔓,等.茶梅和红星茵芋盆栽基质的筛选[J].林业科技开发,2007,21(4):70-72.
[7]郑天汉,李勇,吴远彬,等.茵芋驯化栽培与植物生长调节剂促进效应研究[J].天津农业科学,2016,22(8):125-129.
[8]江斌,张文融,陈碧华,等.红星茵芋组织培养技术研究[J].中国园艺文摘,2016,32(3):12-13,21.
[9]孟艳琼,李仁杰,伊兴凯,等.红星茵芋离体花梗腋芽诱导及丛生芽增殖研究[J].中国农学通报,2007,23(11):81-85.
[10]黄益基,吕秀立,付瑞,等.金红茵芋离体快速繁殖技术的研究[J].江苏林业科技,2017,44(4):8-12,17.
[11]PONCHIA G,ZANIN G.In vitro propagation of Skimmia(Skimmia japonica Thunb.)[J].Acta Horticulturae,2000,520(520):91-96.
[12]闫国华,周宇,张晓明,等.植物离体培养中的顽拗现象及其生理和遗传基础[J].植物生理学通讯,2002,38(5):481-486.