壮精合剂改善东莨菪碱小鼠模型认知功能的机制
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摘要
目的:探讨壮精合剂对东莨菪碱小鼠模型的学习记忆能力及胆碱能功能的影响。方法:应用腹腔注射东莨菪碱的方法制备东莨菪碱小鼠模型,各组小鼠给予相应药液灌胃1周后,应用Morris水迷宫试验检测各组小鼠学习记忆能力的变化,并测定小鼠脑组织中乙酰胆碱(Ach)、乙酰胆碱酯酶(AchE)及胆碱乙酰转移酶(ChAT)含量的变化。结果:壮精合剂高剂量组能显著改善东莨菪碱小鼠模型的学习记忆能力(P<0.01),并提高其脑组织中Ach(P<0.01)和ChAT(P<0.01)的含量。结论:壮精合剂可以在一定程度上改善东莨菪碱小鼠模型的学习记忆能力,壮精合剂可能通过调节东莨菪碱小鼠模型的胆碱能系统,进而改善其学习记忆能力。
Objective: To elucidate the mechanisms of Zhuang Jing Decoction(ZJD) on cognitive function and Cholinergic in Scopolamine mice. Methods: The scopolamine mice models were prepared by using scopolamine intraperitoneal injection. Mice were given the corresponding drug solution gavage daily. After 1 week, Morris water maze test was carried out to evaluate the learning and memory abilities in mice. The levels of Acetyl Choline(Ach), Acetyl Cholinesterase(AchE) activity and Choline Acetyltransferase(ChAT) in brain tissues were detected. Results: High dose of ZJD could improve the learning and memory abilities in scopolamine mice(P<0.01). In addition, the levels of Ach(P<0.01) and ChAT(P<0.01) in brain tissues of scopolamine mice were increased. Conclusion: ZJD could improve the learning and memory abilities in scopolamine mice. This effect may through regulating the cholinergic function in scopolamine mice.
Objective: To elucidate the mechanisms of Zhuang Jing Decoction(ZJD) on cognitive function and Cholinergic in Scopolamine mice. Methods: The scopolamine mice models were prepared by using scopolamine intraperitoneal injection. Mice were given the corresponding drug solution gavage daily. After 1 week, Morris water maze test was carried out to evaluate the learning and memory abilities in mice. The levels of Acetyl Choline(Ach), Acetyl Cholinesterase(AchE) activity and Choline Acetyltransferase(ChAT) in brain tissues were detected. Results: High dose of ZJD could improve the learning and memory abilities in scopolamine mice(P<0.01). In addition, the levels of Ach(P<0.01) and ChAT(P<0.01) in brain tissues of scopolamine mice were increased. Conclusion: ZJD could improve the learning and memory abilities in scopolamine mice. This effect may through regulating the cholinergic function in scopolamine mice.
引文
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[17]Auld D S,Kornecook T J,Bastianetto S,et al.Alzheimer’s disease and the basalforebrain cholinergic system:Relations to betaamyloid peptides,cognition,andtreatment strategies.Prog Neurobiol,2002,68(3):209-211
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[2]Selkoe D J.Alzheimer disease mechanistic understanding presicts novel therapies.An Intern Med,2004,140:627-638
[3]蔡浩斌,黄忠仕,黄岑汉.壮精合剂对初老大鼠行为学及脑单胺类神经递质的影响.重庆医学,2015,44(2):165-167,170
[4]蔡浩斌,黄忠仕,黄岑汉.壮精合剂对初老大鼠学习记忆能力和海马区神经营养因子的影响.右江民族医学院学报,2013,35(6):766-768
[5]World Health Organization.Alzheimer’s Disease International(2012)Dementia:A public health priority.World Health Organization,2012:19-21
[6]Naghavi M,Wang H,Lozano R,et al.Global,regional,and national age-sex specific all-cause and cause-specific mortality for 240causes of death,1990-2013:A systematic analysis for the global burden of disease study 2013.Lancet,2015,385:117-171
[7]Weiner M W,Veitch D P,Aisen P S,et al.2014 update of the alzheimer’s disease neuroimaging initiative:A review of papers published since its inception.Alzheimer’s&Dementia:The Journal of The Alzheimer’s Association,2015,11(6):e1-e120
[8]俞厚明,俞一超,张小平.小剂量多奈哌齐与美金刚合用治疗阿尔茨海默病的有效性及安全性.中国老年学杂志,2015,35(17):4848-4850
[9]杨南竹,贠相华,周玉颖,等.多奈哌齐治疗阿尔茨海默病的系统评价.中华老年心脑血管病杂志,2015,17(1):61-66
[10]Salomone S,Caraci F,Leggio G M,et al,New pharmacological strategies for treatmentof Alzheimer’s disease:Focus on disease modifying drugs.Br J Clin Pharmacol,2012,73(4):504-516
[11]杨荣禄,杨帆,吴松立,等.中医治疗轻度认知损害的临床研究进展.中华中医药杂志,2018,33(10):4548-4551
[12]蔡浩斌,潘华峰,郑浩涛,等.脑髓康通过调控氧化应激及单胺类神经递质改善VCI大鼠情景记忆能力.中药新药与临床药理,2018,29(5):564-567
[13]罗晓莉.银杏叶提取物对东莨菪碱致小鼠学习记忆障碍的作用.南宁:广西师范大学,2013
[14]王莉,王虎,黄翰宇,等.阿尔茨海默病发病机制的研究进展.中国老年学杂志,2017,37(19):4953-4954
[15]于洋,万莉红.治疗阿尔茨海默病的药物研究进展.四川生理科学杂志,2017,39(4):232-235
[16]温蒲圆,周军.阿尔茨海默病治疗的研究进展.中国老年学杂志,2013,33(14):3524-3527
[17]Auld D S,Kornecook T J,Bastianetto S,et al.Alzheimer’s disease and the basalforebrain cholinergic system:Relations to betaamyloid peptides,cognition,andtreatment strategies.Prog Neurobiol,2002,68(3):209-211
[18]Anand P,Singh B.A review on cholinesterase inhibitors for Alzheimer’s disease.Arch Pharm Res,2013,24(6):50-52