纤维素酶及乳酸菌对棉花秸秆青贮饲料发酵品质及体外消化率的影响
摘要
本试验旨在探讨纤维素酶和乳酸菌对棉花秸秆青贮饲料发酵品质及体外消化率的影响,采用裹包青贮技术,在不添加或添加纤维素酶的条件下分别设置对照(CK)、添加植物乳杆菌(LP)和干酪乳杆菌(LC)共6个青贮处理,发酵60 d后取各处理样品进行相关指标的测定和分析。结果表明:青贮处理可有效降低棉花秸秆中的游离棉酚含量;添加纤维素酶可显著降低棉花秸秆青贮饲料的pH、中性洗涤纤维、酸性洗涤纤维和半纤维素(P <0.05),添加乳酸菌则显著增加棉花秸秆青贮饲料中的乳酸、乙酸、丙酸和V-score(P <0.05);乳酸菌、纤维素酶和乳酸菌的互作效应可显著增加棉花秸秆青贮饲料的体外粗蛋白消化率、体外中性洗涤纤维消化率和产气量(P <0.05)。综合各指标来看,以添加纤维素酶的植物乳杆菌处理获得的棉花秸秆青贮饲料发酵品质较好,且48 h累积产气量最高。
引文
[1]高静.棉花秸秆木质纤维素降解菌的筛选及鉴定[D].阿拉尔:塔里木大学,2 012.
[2]热沙来提汗·买买提.化学及生物处理对棉花秸秆消化性改善效果研究[D].乌鲁木齐:新疆农业大学,2012.
[3]张永辉,严萍,樊振,等.新疆棉花秸秆中乳酸菌的分离与鉴定[J].饲料工业,2011,32(17):36-39.
[4]哈丽代·热合木江,古丽娜尔·吐尔逊尼亚孜,哈斯亚提·托逊江,等.棉花秸秆颗粒饲料特性的研究[J].草食家畜,2014(1):50-53.
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[6]赛买提·艾买提,欧阳宏飞,赵丽,等.五种不同方法对棉副产品棉酚脱毒效果的比较研究[J].饲料工业,2008,29(1):27-30.
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[19]王中华,魏晓静.不同添加剂玉米秸青贮的瘤胃降解率及饲养肥育架子牛的效果[J].中国畜牧杂志,2002,38(2):25-26.
[20]王目森.植物乳杆菌与布氏乳杆菌对多花黑麦草青贮品质及有氧稳定性的影响[D].雅安:四川农业大学,2015.
[21]乌斯满·依米提,买尔哈巴·艾合买提,热孜弯古·雪合热提.纤维素分解菌对棉花秸秆纤维素含量的影响[J].安徽农业科学,2013,41(35):13489-13491.
[22]席琳乔.棉花秸秆木质纤维素降解及其青贮复合菌系的筛选和协同机理研究[D].哈尔滨:东北林业大学,2014.
[23]刘东山,刘家平,张运海,等.棉花秸秆微贮加工技术[J].新疆畜牧业,2016(12):59-60.
[24]张苏江,孙俊奇.纤维素酶对棉花秸秆发酵的影响[J].安徽农业科学,2007,35(4):976-977.
[25]曹瑞华,霍文婕,郭刚,等.纤维素酶对氨化小麦秸秆体外发酵特性及甲烷产量的影响[J].粮食与饲料工业,2017(6):50-53.
[26]陈亮,任傲,李斌,等.植物乳杆菌对玉米秸秆和水稻秸秆体外发酵特性的影响[J].动物营养学报,2017,29(2):678-689.
[27]刘晶晶,刘小平,师建芳,等.高温分解与乳酸菌分步发酵提高秸秆饲料消化率及适口性[J].农业工程学报,2014,30(22):290-299.
[28]邢智华,魏荣荣,闫宏.添加乳酸菌对稻草与苜蓿混合微贮消化率的影响[J].畜牧与兽医,2015,47(2):44-46.
[29]张继泉,孙玉英,关凤梅,等.玉米秸秆纤维素酶解条件的初步研究[J].粮食与饲料工业,2002(4):21-23.
[2]热沙来提汗·买买提.化学及生物处理对棉花秸秆消化性改善效果研究[D].乌鲁木齐:新疆农业大学,2012.
[3]张永辉,严萍,樊振,等.新疆棉花秸秆中乳酸菌的分离与鉴定[J].饲料工业,2011,32(17):36-39.
[4]哈丽代·热合木江,古丽娜尔·吐尔逊尼亚孜,哈斯亚提·托逊江,等.棉花秸秆颗粒饲料特性的研究[J].草食家畜,2014(1):50-53.
[5]刘艳丰,唐淑珍,桑断疾.新疆棉花秸秆作为饲料资源的利用开发现状[J].中国奶牛,2009(9):27-30.
[6]赛买提·艾买提,欧阳宏飞,赵丽,等.五种不同方法对棉副产品棉酚脱毒效果的比较研究[J].饲料工业,2008,29(1):27-30.
[7]许宗运,张锐,张玲,等.棉秆不同微贮方法效果研究[J].中国草食动物,1999,1(4):22-24.
[8]张霞,付晓悦,王虎成,等.饲用甜高粱裹包青贮料对肉羊生产性能及血清指标的影响[J].动物营养学报,2018(5):1771-1780.
[9]Zhang Q,Zhao M,Wang X,et al.Ensiling alfalfa with whole crop corn improves the silage quality and in vitro digestibility of the silage mixtures[J].Grassl Sci,2017,63(4):211-217.
[10]Zhang Q,Li X,Zhao M,et al.Lactic acid bacteria strains for enhancing the fermentation quality and aerobic stability of Leymus chinensis silage[J].Grass Forage Sci,2016,71(3):472-481.
[11]Broderick G,Kang J.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media1[J].J Dairy Sci,1980,63(1):64-75.
[12]Takahashi T,Horiguchi K,Goto M.Effect of crushing unhulled rice and the addition of fermented juice of epiphytic lactic acid bacteria on the fermentation quality of whole crop rice silage,and its digestibility and rumen fermentation status in sheep[J].Anim Sci J,2005,76(4):353-358.
[13]王永力.新疆乌拉尔甘草茎叶饲料特性及饲喂效果研究[D].乌鲁木齐:新疆农业大学,2015.
[14]France J,Dijkstra J,Dhanoa M,et al.Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro:derivation of models and other mathematical considerations[J].Br J Nutr,2000,83(2):143-150.
[15]Carro M,Ranilla M,Tejido M.Using an in vitro gas production technique to examine feed additives:Effects of correcting values for different blanks[J].Anim Feed Sci Tech,2005,123:173-184.
[16]Selmer-Olsen I.Enzymes as silage additives for grass-clover mixtures[J].Grass Forage Sci,1994,49(3):305-315.
[17]McDonald P.Animal Nutrition[M].Washington,D.C.:Pearson Education,2002.
[18]郭金双,赵广永.添加乳酸菌对大麦青贮品质及中,酸性洗涤纤维的瘤胃降解率的影响[J].中国畜牧杂志,1999,35(4):27-28.
[19]王中华,魏晓静.不同添加剂玉米秸青贮的瘤胃降解率及饲养肥育架子牛的效果[J].中国畜牧杂志,2002,38(2):25-26.
[20]王目森.植物乳杆菌与布氏乳杆菌对多花黑麦草青贮品质及有氧稳定性的影响[D].雅安:四川农业大学,2015.
[21]乌斯满·依米提,买尔哈巴·艾合买提,热孜弯古·雪合热提.纤维素分解菌对棉花秸秆纤维素含量的影响[J].安徽农业科学,2013,41(35):13489-13491.
[22]席琳乔.棉花秸秆木质纤维素降解及其青贮复合菌系的筛选和协同机理研究[D].哈尔滨:东北林业大学,2014.
[23]刘东山,刘家平,张运海,等.棉花秸秆微贮加工技术[J].新疆畜牧业,2016(12):59-60.
[24]张苏江,孙俊奇.纤维素酶对棉花秸秆发酵的影响[J].安徽农业科学,2007,35(4):976-977.
[25]曹瑞华,霍文婕,郭刚,等.纤维素酶对氨化小麦秸秆体外发酵特性及甲烷产量的影响[J].粮食与饲料工业,2017(6):50-53.
[26]陈亮,任傲,李斌,等.植物乳杆菌对玉米秸秆和水稻秸秆体外发酵特性的影响[J].动物营养学报,2017,29(2):678-689.
[27]刘晶晶,刘小平,师建芳,等.高温分解与乳酸菌分步发酵提高秸秆饲料消化率及适口性[J].农业工程学报,2014,30(22):290-299.
[28]邢智华,魏荣荣,闫宏.添加乳酸菌对稻草与苜蓿混合微贮消化率的影响[J].畜牧与兽医,2015,47(2):44-46.
[29]张继泉,孙玉英,关凤梅,等.玉米秸秆纤维素酶解条件的初步研究[J].粮食与饲料工业,2002(4):21-23.