刺参低聚肽对糖尿病小鼠降血糖作用的研究
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摘要
研究刺参低聚肽对四氧嘧啶致糖尿病小鼠的降血糖作用。腹腔注射四氧嘧啶造小鼠糖尿病模型,连续6周灌胃不同剂量的刺参低聚肽(0.1、0.2、0.5 g/kg),记录灌胃0、2、4、6周后的小鼠体重及空腹血糖值,并测定灌胃结束后的小鼠胸腺、脾脏、肝脏和肾脏指数,以及血清丙二醛(malondialdehyde,MDA)含量与血清超氧化物歧化酶(superoxide dismutase,SOD)活性。刺参低聚肽中、高剂量能减缓糖尿病小鼠消瘦的症状(p<0.01),降低糖尿病小鼠的血糖值(p<0.05),且作用浓度与时间成正相关。高剂量的刺参低聚肽能显著提高小鼠各脏器指数(p<0.05),降低糖尿病小鼠血清MDA含量(p<0.05),提高血清SOD活性(p<0.01)。刺参低聚肽对糖尿病小鼠具有降血糖、增强免疫力、保护肝肾及抗氧化的作用,推测可能通过抗氧化和增强免疫力起到降低血糖及防治糖尿病并发症的作用。
The effects of Apostichopus japonicus oligo-peptides on alloxan-induced diabetic mice were studied.The diabetic mice were induced by intraperitoneal injection of alloxan,and administered by oral gavage at different dose of Apostichopus japonicus oligo-peptides(0.1,0.2,0.5 g/kg)for 6 weeks. The body weight and content of fasting blood glucose(Glu)were detected on 0,2,4,6 weeks. The index of thymus,spleen,liver and kidney,the content of malondialdehyde(MDA)and the activity of superoxide dismutase(SOD)in serum were measured after last administration. The loss of body weight was obviously relieved(p <0.01) and the content of Glu were decreased(p<0.05)at middle and high dose of Apostichopus japonicus oligo-peptides. The concentration was positively correlated with the time. The high dose of Apostichopus japonicus oligo-peptides significantly increased the organ index of diabetic mice(p<0.05),decreased the content of MDA(p<0.05),and increased the activity of SOD in serum(p<0.01). Apostichopus japonicus oligo-peptides can reduce the content of Glu,improve immunity and oxidative activity,and protect liver and kidney. It can infer that Apostichopus japonicus oligo-peptides reduce the content of Glu and prevent diabetic complications by increasing oxidative activity and immunity.
The effects of Apostichopus japonicus oligo-peptides on alloxan-induced diabetic mice were studied.The diabetic mice were induced by intraperitoneal injection of alloxan,and administered by oral gavage at different dose of Apostichopus japonicus oligo-peptides(0.1,0.2,0.5 g/kg)for 6 weeks. The body weight and content of fasting blood glucose(Glu)were detected on 0,2,4,6 weeks. The index of thymus,spleen,liver and kidney,the content of malondialdehyde(MDA)and the activity of superoxide dismutase(SOD)in serum were measured after last administration. The loss of body weight was obviously relieved(p <0.01) and the content of Glu were decreased(p<0.05)at middle and high dose of Apostichopus japonicus oligo-peptides. The concentration was positively correlated with the time. The high dose of Apostichopus japonicus oligo-peptides significantly increased the organ index of diabetic mice(p<0.05),decreased the content of MDA(p<0.05),and increased the activity of SOD in serum(p<0.01). Apostichopus japonicus oligo-peptides can reduce the content of Glu,improve immunity and oxidative activity,and protect liver and kidney. It can infer that Apostichopus japonicus oligo-peptides reduce the content of Glu and prevent diabetic complications by increasing oxidative activity and immunity.
引文
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[2]赵学敏.本草纲目拾遗[M].北京:商务印书馆,1955:495
[3]钟志贵.海参为主治疗糖尿病[J].中医杂志,1989(12):55
[4]王静凤,傅佳,徐雷雷,等.日本刺参降血糖及对胰岛素信号通路的影响[J].深圳大学学报理工版,2011,28(2):172-177
[5]张昕,赵延蕾,陈睿曦,等.海参岩藻聚糖硫酸酯对小鼠胰岛素抵抗及炎症因子的影响[J].营养卫生,2014,35(21):201-206
[6]胡世伟,王静凤,王玉明,等.常耀光海地瓜岩藻糖基化海参硫软骨素对Ⅱ型糖尿病小鼠胰岛素抵抗改善作用的研究[J].中国海洋药物,2014,33(1):58-64
[7]徐雷雷,王静凤,柳东,等.海参磷脂型二十碳五烯酸对糖尿病大鼠血葡萄糖耐量的影响[J].中国药理学通报,2013,28(10):1398-1420
[8]何丽霞,李勇.海参肽生物学功能研究进展[J].食品科学,2015,36(9):215-218
[9]朱文丽,王春蕾,董辛欣,等.海洋胶原肽对糖尿病大鼠脂代谢和抗氧化水平的影响[J].现代预防医学,2010,37(8):1431-1434
[10]孙彬,李迪,毛瑞雪,等.吉林人参低聚肽对糖尿病大鼠的降血糖作用研究[J].中国食物与营养,2016,22(10):62-65
[11]陈锐,陈德经,张建新.西洋参多糖肽对糖尿病小鼠降血糖血脂及抗氧化作用研究[J].西北农业学报,2013,22(11):195-201
[12]刘欣然,刘思奇,侯超,等.燕麦低聚肽对糖尿病大鼠血糖的影响[J].中国食物与营养,2018,24(4):46-50
[13]王美华,查保国,许敏.海参肽与海带多糖对小鼠血糖水平的影响[J].中国中医药现代远程教育,2015,13(24):145-146
[14]董丽莎,李妍妍,张红燕,等.两种海参多肽对糖尿病大鼠肾脏90的防护作用研究[J].食品工业科技,2017,38(9):343-348
[15]王天星,李勇,李迪,等.海参肽对db/db小鼠降糖作用和炎症反应程度的影响[J].中国食物与营养,2018,24(4):51-55
[16]中华人民共和国卫生部.保健食品检验与评价技术规范[M].北京:化学工业出版社,2003:87-93
[17]严奉伟,严赞开,王辰,等.菜籽多糖对四氧嘧啶致糖尿病小鼠降血糖作用的研究[J].食品研究与开发,2007,28(3):11-14
[18]卢纹岱.SPSS for Windows统计分析[M].北京:电子工业出版社,2002:124-140
[19]林子桐,张超,沈雪梅.糖尿病肾病发病机制研究进展[J].中国药理学与毒理学杂志,2014,28(5):765-773
[20]曹娜娜,蒋玲.糖尿病慢性并发症发病机制研究进展[J].山东医药,2008,48(10):102-103