高精度定量毛细管电泳法同时测定复合维生素B片中B_1、B_2、B_6、烟酰胺及泛酸钙
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摘要
建立了高精度定量毛细管电泳法同时测定复合维生素B片中维生素B_1、B_2、B_6、烟酰胺和泛酸钙的方法。样品经乙腈-水(20∶80, v/v)超声提取后,采用全自动高精度定量毛细管电泳仪,以高精度进样阀定量进样,以40 mmol/L硼砂-硼酸缓冲液(pH 9.0)为背景电解质溶液,以工作电压为-10 kV电泳分离。维生素B_1、B_2、B_6、烟酰胺的检测波长为280 nm,然后切换至检测波长210 nm检测泛酸钙。结果表明,各组分之间均得到良好分离,峰面积日内重复性(RSD)为1.3%～1.9%,显著优于普通毛细管电泳。维生素B_1、B_2、B_6、烟酰胺及泛酸钙的浓度在各自线性范围内的相关系数(r)为0.996 8～0.999 8,检出限2.5～36.0 mg/L,平均回收率为94.1%～98.9%。该法准确可靠,可用于实际复合维生素B片中维生素B_1、B_2、B_6、烟酰胺和泛酸钙含量的同时测定。
A high performance quantitative capillary electrophoresis method was developed for the simultaneous determination of vitamins B_1, B_2, B_6, nicotinamide, and calcium pantothenate in vitamin B tablets using a quantitative capillary electrophoresis instrument. The samples were extracted ultrasonically with acetonitrile-water(20∶80, v/v). The automatic high precision quantitative capillary electrophoresis instrument was used to realize quantitative injection through a 10 nL injection valve. The background electrolyte was selected as 40 mmol/L sodium borate buffer(pH 9.0), which was continuously supplied by a microfluidic injection pump. The working voltage was-10 kV. The detection wavelength of vitamins B_1, B_2, B_6, and nicotinamide was selected as 280 nm, which was then changed to 210 nm to detect calcium pantothenate. The result showed good linearities between the peak area and the concentration of vitamins B_1, B_2, B_6, nicotinamide, and calcium pantothenate in the correlation coefficients(r) range of 0.996 8-0.999 8. The limits of detection(LODs) were in the range of 2.5-36.0 mg/L. The average recoveries were 94.1%-98.9% with relative standard deviations(RSDs) as 1.3%-1.9%. The method is precise, reliable, and suitable for the simultaneous determination of vitamins B_1, B_2, B_6, nicotinamide, and calcium pantothenate in a real compound vitamin B tablet.
A high performance quantitative capillary electrophoresis method was developed for the simultaneous determination of vitamins B_1, B_2, B_6, nicotinamide, and calcium pantothenate in vitamin B tablets using a quantitative capillary electrophoresis instrument. The samples were extracted ultrasonically with acetonitrile-water(20∶80, v/v). The automatic high precision quantitative capillary electrophoresis instrument was used to realize quantitative injection through a 10 nL injection valve. The background electrolyte was selected as 40 mmol/L sodium borate buffer(pH 9.0), which was continuously supplied by a microfluidic injection pump. The working voltage was-10 kV. The detection wavelength of vitamins B_1, B_2, B_6, and nicotinamide was selected as 280 nm, which was then changed to 210 nm to detect calcium pantothenate. The result showed good linearities between the peak area and the concentration of vitamins B_1, B_2, B_6, nicotinamide, and calcium pantothenate in the correlation coefficients(r) range of 0.996 8-0.999 8. The limits of detection(LODs) were in the range of 2.5-36.0 mg/L. The average recoveries were 94.1%-98.9% with relative standard deviations(RSDs) as 1.3%-1.9%. The method is precise, reliable, and suitable for the simultaneous determination of vitamins B_1, B_2, B_6, nicotinamide, and calcium pantothenate in a real compound vitamin B tablet.
引文
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[15] Boonkerd S,Detaevernier M R,Michotte Y.J Chromatogr A,1994,670:209
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