γ射线外照射对大黄鱼幼鱼生长的影响
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摘要
电离辐射对海洋生物的影响日益受到人们关注。为探讨电离辐射对沿海重要水产经济物种大黄鱼(Pseudosciaena crocea)幼鱼生长的影响,通过不同剂量(0, 4, 8, 16, 32 Gy)的~(60)Coγ射线外照射,分别从存活、体重体长、抗氧化能力和消化能力等几方面,观察分析了大黄鱼幼鱼生长的辐射响应情况。结果表明,不同剂量的γ射线照射对养殖期间的大黄鱼幼鱼体重、体长和器官指数的影响均不显著;对大黄鱼幼鱼抗氧化能力及消化能力的刺激/抑制表现状况差异明显,其中,低剂量组在照射后前期有显著促进作用,但随养殖时间增加趋于正常,中、高剂量组在养殖周期内均呈抑制状态,并造成鱼体不可逆的损伤。该实验结果为海洋生物的辐射效应研究提供数据支撑,为滨海核电周边海域环境放射性安全评估提供科学依据。
The effects of ionizing radiation on marine creatures have received increasing attention. In order to investigate the effects of ionizing radiation on the growth of juvenile Pseudosciaena crocea, an important aquatic commercial species in China,~(60) Co gamma-ray external irradiation with different doses(0, 4, 8, 16, 32 Gy) was employed in this study. Then the survival, body weight, body length, antioxidant capacity and digestive capacity of this species were observed and analyzed in this study. The results showed that different doses of γ-ray irradiation had no significant effect on body weight, body length and organ index of juvenile Pseudosciaena crocea. The stimulation/inhibition performance of other growth features was present to be significantly different during the whole incubation. The significant promotion effect was present in the early stage of the low dose group of irradiation and tended to be normal following the incubation, and inhibition effects and irreversible body damage was observed in the medium and high dose groups. The results can shed light on the future study of the radiation effects on marine organisms and the development of environmental radioactivity safety assessment in the coastal waters surrounding the nuclear power.
The effects of ionizing radiation on marine creatures have received increasing attention. In order to investigate the effects of ionizing radiation on the growth of juvenile Pseudosciaena crocea, an important aquatic commercial species in China,~(60) Co gamma-ray external irradiation with different doses(0, 4, 8, 16, 32 Gy) was employed in this study. Then the survival, body weight, body length, antioxidant capacity and digestive capacity of this species were observed and analyzed in this study. The results showed that different doses of γ-ray irradiation had no significant effect on body weight, body length and organ index of juvenile Pseudosciaena crocea. The stimulation/inhibition performance of other growth features was present to be significantly different during the whole incubation. The significant promotion effect was present in the early stage of the low dose group of irradiation and tended to be normal following the incubation, and inhibition effects and irreversible body damage was observed in the medium and high dose groups. The results can shed light on the future study of the radiation effects on marine organisms and the development of environmental radioactivity safety assessment in the coastal waters surrounding the nuclear power.
引文
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[20]丁玲玲.日本Medaka鱼在正常实验室环境及辐射暴露下主要器官的衰老进程[D].武汉:武汉大学,2011:1-4Ding L L.Aging of Japanese Medaka fish under normal and radiation exposure conditions[D].Wuhan:Wuhan University,2011:1-4(in Chinese)
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[23]Zhou R,Song J,Si J,et al.Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos[J].Mutation Research,2016,793-794:41-50
[24]Zhou R,Zhang H.3-98 oxidative stress12C6+ion irradiation effect and damage of zebrafish eyes[J].IMP&HIRFL Annual Report,2012,1:191
[25]赵卫平,张红,邱嵘,等.碳离子全身辐照对小鼠血清及肝脏中超氧化物歧化酶活性、丙二醛含量和脑组织中还原性谷胱甘肽浓度的影响[J].辐射研究与辐射工艺学报,2007,25(1):33-36Zhao W P,Zhang H,Qiu R,et al.Superoxide dismutase activity and maleic dialdehyde content in serum and liver and glutathione level in brain of12C6+ions-irradiated mice[J].Journal of Radiation Research and Radiation Processing,2007,25(1):33-36(in Chinese)
[26]Valavanidis A,Vlahogianni T,Dassenakis M,et al.Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants[J].Ecotoxicology&Environmental Safety,2006,64(2):178-189
[27]Gomes T,Song Y,Brede D A,et al.Gamma radiation induces dose-dependent oxidative stress and transcriptional alterations in the freshwater crustacean Daphnia magna[J].Science of The Total Environment,2018,628:206-216
[28]邓海平,俞诗源,陈玉琴,等.X射线辐射对仔鼠体重、皮毛生长及肝、肾脏组织SOD、CAT活力及MDA含量的影响[J].动物学报,2008,54(6):1029-1037Deng H P,Yu S Y,Chen Y Q,et al.Effects of X-ray on postnatal body weight,growth of skin and hair,activities and content of superoxide dismutase,catalase,and maleic dialdehyde in liver and kidney of mice[J].Current Zoology,2008,54(6):1029-1037(in Chinese)
[29]刘亚宁.电磁辐射生物效应的机理研究述评[J].基础医学与临床,2000,20(1):20-23Liu Y N.Review on the investigation of the mechanism of the biological effects of the electromagnetic radiation[J].Basic Medical Sciences and Clinics,2000,20(1):20-23(in Chinese)
[30]任春兰,陆建峰,白洁.还原型谷胱甘肽微量测定法的研究[J].毒理学杂志,2003,17(4):245-246
[31]Olsvik P A,Heier L S,Rosseland B O,et al.Effects of combined gamma-irradiation and metal(Al+Cd)exposures in Atlantic salmon(Salmo salar L.)[J].Journal Environmental Radioactivity,2010,101(3):230-236
[32]袁成福.硒茶和非硒茶对D-半乳糖致衰老小鼠总抗氧化能力的对照研究[J].湖北民族学院学报:医学版,2004,21(3):51-53Yuan C F.To contrast the effects of selenium tea with nonselenium tea on total antioxidative capacity in aging mice induced with D-galactose[J].Journal of Hubei Institute for Nationalities:Medical Edition,2004,21(3):51-53(in Chinese)
[33]Augustine S,Pereir A S,Floriani M,et al.Effects of chronic exposure to environmentally relevant concentrations of waterborne depleted uranium on the digestive tract of zebrafish,Danio rerio[J].Journal Environmental Radioactivity,2015,142(9):45-53
[34]邱杨玉.大黄鱼内脏白点病的研究[D].武汉:华中农业大学,2012:1-4Qiu Y Y.Study on white-sports disease in internal organs of Pseudosciaena crocea[D].Wuhan:Huazhong Agricultural University,2012:1-4(in Chinese)
[35]肖英平,刘玮,胡向萍,等.鱼类胰蛋白酶研究进展[J].南昌师范学院学报,2011,32(3):34-38Xiao Y P,Liu W,Hu X P,et al.Ontrypsin in fish[J].Journal of Nanchang Normal University,2011,32(3):34-38(in Chinese)
[2]Valentin J E.ICRP Publication 103:The 2007 recommendations of the International Commission on Radiological Protection[J].Annals of the ICRP,2007,37(2-4):1-332
[3]国家环境保护总局.中华人民共和国放射性污染防治法[J].中国安全生产科学技术,2003,34(4):6-9
[4]环境保护部.核安全与放射性污染防治“十二五”规划及2020年远景目标[M].北京:科学出版社,2013
[5]叶素芬,张珞平,陈伟琪.海洋放射性污染生态风险评价研究进展[J].生态毒理学报,2016,11(6):1-11Ye S F,Zhang L P,Chen W Q.Progress of ecological risk assessment for marine radioactive pollution[J].Asian Journal of Ecotoxicology,2016,11(6):1-11(in Chinese)
[6]郑钧正.联合国原子辐射效应科学委员会及其报告书概览[J].辐射防护通讯,2011,31(6):32-38
[7]夏寿萱.放射生物学[M].北京:军事医学科学出版社,1998:10-15
[8]韩宝华.电离辐射影响评价中的生物剂量率限值[J].辐射防护通讯,2013(5):1-5Han B H.The dose rate limit of biota for assessment of environmental impact of ionizing radiation[J].Radiation Protection Bulletin,2013(5):1-5(in Chinese)
[9]周永增.非人类物种的辐射生物效应及其评价[J].辐射防护通讯,2004,24(4):1-9Zhou Y Z.Biological effect of radiation on non-human species[J].Radiation Protection Bulletin,2004,24(4):1-9(in Chinese)
[10]Embry M R,Belanger S E,Braunbeck T A,et al.The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research[J].Aquatic Toxicology,2010,97(2):79-87
[11]王玉佩,张红,周鑫,等.斑马鱼胚胎在电离辐射生物学研究中的应用[J].原子核物理评论,2016,33(1):94-104Wang Y P,Zhang H,Zhou X,et al.Applications of zebrafish embryo in ionizing radiation biology[J].Nuclear Physics Review,2016,33(1):94-104(in Chinese)
[12]彭小明.贝类辐射生物学效应的初步研究[D].北京:中国科学院研究生院,2004:1-8Peng X M.Preliminary studies on the radiation biology effect of molluscs[D].Beijing:Graduate University of Chinese Academy of Sciences,2004:1-8(in Chinese)
[13]Won E J,Dahms H U,Kumar K S,et al.An integrated view of gamma radiation effects on marine fauna:From molecules to ecosystems[J].Environmental Science and Pollution Research,2015,22(22):17443-17452
[14]朱振乐.大黄鱼人工育苗技术总结[J].水产学杂志,2000,13(1):30-34Zhu Z L.A summary on technique for artificial rearing of Pseudosciaena crocea(Richardson)[J].Chinese Journal of Fisheries,2000,13(1):30-34(in Chinese)
[15]刘家富,韩坤煌.我国大黄鱼产业的发展现状与对策[J].渔业研究,2011,33(5):4-8Liu J F,Han K H.Current development situation and countermeasure of large yellow crocker industry in China[J].Journal of Fisheries Research,2011,33(5):4-8(in Chinese)
[16]郭建波.重金属(铬、镍、砷)对大黄鱼幼鱼的毒性和毒理学研究[D].舟山:浙江海洋大学,2017:1-2Guo J B.Toxicity and toxicology studies on heavy metals(Cr6+,Ni2+,As3+)in juvenile Pseudosciaena crocea[D].Zhoushan:Zhejiang Ocean University,2017:1-2(in Chinese)
[17]翁朝红,王志勇,谢芳靖,等.操作胁迫对大黄鱼幼鱼的影响[J].中国水产科学,2008,15(1):100-105Weng Z H,Wang Z Y,Xie F J,et al.Effect of handling stress on fingerlings of large yellow croaker(Pseudosciaena crocea Richardson)[J].Journal of Fishery Sciences of China,2008,15(1):100-105(in Chinese)
[18]Heier L S,Teien H C,Oughton D,et al.Sublethal effects in Atlantic salmon(Salmo salar)exposed to mixtures of copper,aluminium and gamma radiation[J].Journal of Environmental Radioactivity,2013,121(4):33-42
[19]付玉荣,王跃,陈淑惠,等.双歧杆菌脂磷壁酸对衰老小鼠脑组织自由基代谢和器官指数的影响[J].中国老年学,2004,24(11):1034-1035
[20]丁玲玲.日本Medaka鱼在正常实验室环境及辐射暴露下主要器官的衰老进程[D].武汉:武汉大学,2011:1-4Ding L L.Aging of Japanese Medaka fish under normal and radiation exposure conditions[D].Wuhan:Wuhan University,2011:1-4(in Chinese)
[21]Hyodo-Taguchi Y,Egami N.Change in dose-survival time relationship after X-irradiation during embryonic development in the fish,Oryzias latipes[J].Journal of Radiation Research,1969,10(10):121-125
[22]Si J,Zhang H,Wang Z,et al.Effects of12C6+ion radiation and ferulic acid on the zebrafish(Danio rerio)embryonic oxidative stress response and gene expression[J].Mutation Research,2013,745(2):26-33
[23]Zhou R,Song J,Si J,et al.Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos[J].Mutation Research,2016,793-794:41-50
[24]Zhou R,Zhang H.3-98 oxidative stress12C6+ion irradiation effect and damage of zebrafish eyes[J].IMP&HIRFL Annual Report,2012,1:191
[25]赵卫平,张红,邱嵘,等.碳离子全身辐照对小鼠血清及肝脏中超氧化物歧化酶活性、丙二醛含量和脑组织中还原性谷胱甘肽浓度的影响[J].辐射研究与辐射工艺学报,2007,25(1):33-36Zhao W P,Zhang H,Qiu R,et al.Superoxide dismutase activity and maleic dialdehyde content in serum and liver and glutathione level in brain of12C6+ions-irradiated mice[J].Journal of Radiation Research and Radiation Processing,2007,25(1):33-36(in Chinese)
[26]Valavanidis A,Vlahogianni T,Dassenakis M,et al.Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants[J].Ecotoxicology&Environmental Safety,2006,64(2):178-189
[27]Gomes T,Song Y,Brede D A,et al.Gamma radiation induces dose-dependent oxidative stress and transcriptional alterations in the freshwater crustacean Daphnia magna[J].Science of The Total Environment,2018,628:206-216
[28]邓海平,俞诗源,陈玉琴,等.X射线辐射对仔鼠体重、皮毛生长及肝、肾脏组织SOD、CAT活力及MDA含量的影响[J].动物学报,2008,54(6):1029-1037Deng H P,Yu S Y,Chen Y Q,et al.Effects of X-ray on postnatal body weight,growth of skin and hair,activities and content of superoxide dismutase,catalase,and maleic dialdehyde in liver and kidney of mice[J].Current Zoology,2008,54(6):1029-1037(in Chinese)
[29]刘亚宁.电磁辐射生物效应的机理研究述评[J].基础医学与临床,2000,20(1):20-23Liu Y N.Review on the investigation of the mechanism of the biological effects of the electromagnetic radiation[J].Basic Medical Sciences and Clinics,2000,20(1):20-23(in Chinese)
[30]任春兰,陆建峰,白洁.还原型谷胱甘肽微量测定法的研究[J].毒理学杂志,2003,17(4):245-246
[31]Olsvik P A,Heier L S,Rosseland B O,et al.Effects of combined gamma-irradiation and metal(Al+Cd)exposures in Atlantic salmon(Salmo salar L.)[J].Journal Environmental Radioactivity,2010,101(3):230-236
[32]袁成福.硒茶和非硒茶对D-半乳糖致衰老小鼠总抗氧化能力的对照研究[J].湖北民族学院学报:医学版,2004,21(3):51-53Yuan C F.To contrast the effects of selenium tea with nonselenium tea on total antioxidative capacity in aging mice induced with D-galactose[J].Journal of Hubei Institute for Nationalities:Medical Edition,2004,21(3):51-53(in Chinese)
[33]Augustine S,Pereir A S,Floriani M,et al.Effects of chronic exposure to environmentally relevant concentrations of waterborne depleted uranium on the digestive tract of zebrafish,Danio rerio[J].Journal Environmental Radioactivity,2015,142(9):45-53
[34]邱杨玉.大黄鱼内脏白点病的研究[D].武汉:华中农业大学,2012:1-4Qiu Y Y.Study on white-sports disease in internal organs of Pseudosciaena crocea[D].Wuhan:Huazhong Agricultural University,2012:1-4(in Chinese)
[35]肖英平,刘玮,胡向萍,等.鱼类胰蛋白酶研究进展[J].南昌师范学院学报,2011,32(3):34-38Xiao Y P,Liu W,Hu X P,et al.Ontrypsin in fish[J].Journal of Nanchang Normal University,2011,32(3):34-38(in Chinese)