水稻穗发芽性状的QTL定位
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摘要
利用极易穗发芽的粳稻品系B1923与籼稻品种9311构建F_2群体,采用模拟穗发芽方法测定发芽率,检测穗发芽QTL。结果表明:在F_2群体中共检测到9个穗发芽QTL,分别定位在第1、2、3、4、7、11和12染色体。其中主效QTL qPHS-12位于第12染色体标记K44～W82之间, LOD值为20.68,贡献率为24.97%,高发芽率基因型来自B1923。针对qPHS-12构建BC_1F_2群体,进一步将qPHS-12定位在K44～K187之间。针对第1、2、7染色体的4个QTL,构建F_(2∶3)分离群体, QTL检测发现仅qPHS-2-2能重演。这一研究提示主效QTL qPHS-12在不同年份的2个世代间重演,能稳定遗传;因受遗传背景或不同年份环境条件的影响,穗发芽微效QTL在世代间重演性较差。
B1923, a japonica line easily pre-harvest sprouting, was crossed with indica cultivar 9311 to develop a F_2 population for pre-harvest sprouting QTL analysis. A simulated method was used for the determination of pre-harvest sprouting. According to the QTL intervals of F_2 results, F_(2∶3) lines and BC_1F_2 population, developed from F_2 plants by marker assisted selection, were used for QTL verification. The results were as follow: a total of nine QTLs were detected in F_2 population on chromosomes 1, 2, 3, 4, 7, 11 and 12, respectively. Among them, QTL qPHS-12 was located on the interval of chromosome 12 flanked by the SSR markers K44 and W82, explaining 24.97% phenotypic variation with a LOD score of 20.68, and the genotype with high germination rate was from B1923. For verification of qPHS-12, a BC_1F_2 population was constructed, and QTL mapping showed that the qPHS-12 could be further located between the markers K44 and K187. For other four QTL on the chromosomes 1, 2 and 7, QTL were retested in F_(2∶3) segregating lines, and only the qPHS-2-2 can be detected again. All those showed that the major QTL qPHS-12 can repeat in two generations of different years, which can inherit stably; Due to the influence of genetic background or environmental conditions in different years, the minor QTL is difficult to repeat among generations.
B1923, a japonica line easily pre-harvest sprouting, was crossed with indica cultivar 9311 to develop a F_2 population for pre-harvest sprouting QTL analysis. A simulated method was used for the determination of pre-harvest sprouting. According to the QTL intervals of F_2 results, F_(2∶3) lines and BC_1F_2 population, developed from F_2 plants by marker assisted selection, were used for QTL verification. The results were as follow: a total of nine QTLs were detected in F_2 population on chromosomes 1, 2, 3, 4, 7, 11 and 12, respectively. Among them, QTL qPHS-12 was located on the interval of chromosome 12 flanked by the SSR markers K44 and W82, explaining 24.97% phenotypic variation with a LOD score of 20.68, and the genotype with high germination rate was from B1923. For verification of qPHS-12, a BC_1F_2 population was constructed, and QTL mapping showed that the qPHS-12 could be further located between the markers K44 and K187. For other four QTL on the chromosomes 1, 2 and 7, QTL were retested in F_(2∶3) segregating lines, and only the qPHS-2-2 can be detected again. All those showed that the major QTL qPHS-12 can repeat in two generations of different years, which can inherit stably; Due to the influence of genetic background or environmental conditions in different years, the minor QTL is difficult to repeat among generations.
引文
[1] GROOS C,GAY G.Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white×red grain bread-wheat cross [J].Theoretical and Applied Genetics,2002,104(1):39-47.
[2] 张静,林泽川,曹立勇,等.水稻种子穗发芽与休眠性遗传研究进展 [J].核农学报,2013,27(8):1136-1142.
[3] YOSUKE M,UTAKO Y,TOMOKI H,et al.Genetic dissection of pre-harvest sprouting resistance in an upland rice cultivar [J].Breeding Science Preview,2018,68(2):200-209.
[4] DONG Y J,T SUZUKI E,KAMIUNTEN H,et al.Identification of quantitative trait loci associated with preharvest sprouting resistance in rice (Oryza sativa L.) [J].Field Crops Res,2003,81(2/3):133-139.
[5] 卢丙越.水稻品种强休眠性的定位及遗传解析 [D].南京:南京农业大学,2011.
[6] 罗正良.水稻抗穗发芽主效QTL qPSR8的精细定位及候选基因分析 [D].雅安:四川农业大学,2012.
[7] DU L,XU F,FANG J,et al.Endosperm sugar accumulation caused by mutation of PHS8/ISA1 leads to pre-harvest sprouting in rice [J].Plant Journal,2018,95(3):545-556.
[8] TAKEUCHI Y,LIN S Y,SASAKI T,et al.Fine linkage mapping enables dissection of closely linked quantitative trait loci for seed dormancy and heading in rice [J].Theoretical and Applied Genetics,2003,107(7):1174-1180.
[9] GU X Y,KIANIAN S F,FOLEY M E.Multiple loci and epistases control genetic variation for seed dormancy in weedy rice (Oryza sativa) [J].Genetics,2004,166(3):1503-1516.
[10] SUGIMOTO K,TAKEUCHI Y,EBANA K,et al.Molecular cloning of Sdr4,a regulator involved in seed dormancy and domestication of rice [J].Proc Natl Acad Sci USA,2010,107(13):5792-5797.
[11] GU X Y,MICHAEL E F,DAVID P,et al.Association between seed dormancy and pericarp color is controlled by a pleiotropic gene that regulates abscisic acid and flavonoid synthesis in weedy red rice [J].Genetics,2011,189(4):1515-1524.
[12] YE H,FENG J H,ZHANG L H,et al.Map-based cloning of seed dormancy qSD1-2 indentified a gibberellin synthesis gene regulating the development of endosperm-im-posed dormancy in rice [J].Plant Physiology,2015,169(3):2152-2165.
[13] WANG M,LI W Z,FANG C,et al.Parallel selection on a dormancy gene during domestication of crops from multiple families [J].Nature Genetics,2018,50(10):1435-1441.
[14] POREBSKI S,BAILEY L G,BAUM B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components [J].Plant Mol Biol Rep,1997,15(1):8-15.
[15] SANGUINETTI C J,DIAS N E,SIMPSON A J.RAPD silver staining and recovery of PCR products separated on polyacrylamide gels [J].Biotechniques,1994,17(5):914-918.
[16] MENG L,LI H H,ZHNG L Y,et al.QTL IciMapping:Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations [J].Crop J,2015,3(3):269-283.
[17] LEE S J,OH C S,SUH J P,et al.Identification of QTLs for domestication-related and agronomic traits in an Oryza sativa × O.rufipogon BC1F7 population [J].Plant Breeding,2005,124(3):209-219.
[18] NAGAOKA L,SASAHARA H,TABUCHI H,et al.Improvement of seed shattering and dormancy in Oryza sativa L.‘Hokuriku 193’ based on genetic information [J].Breeding Science,2017,67(3):173-180.
[19] 陈海生,陶龙兴,王熹,等.水稻穗芽相关性状的QTL定位 [J].中国水稻科学,2006,20(3):253-258.
[20] ZHOU Y L,XIE Y H,CAI J L,et al.Substitution mapping of QTLs controlling seed dormancy using single segment substitution lines derived from multiple cultivated rice donors in seven cropping seasons [J].Theoretical and Applied Genetics,2017,130(6):1191-1205.
[21] GUO L B,ZHU L H,XU Y B,et al.QTL analysis of seed dormancy in rice (Oryza sativa L.) [J].Euphytica,2004,140(3):155-162.
[2] 张静,林泽川,曹立勇,等.水稻种子穗发芽与休眠性遗传研究进展 [J].核农学报,2013,27(8):1136-1142.
[3] YOSUKE M,UTAKO Y,TOMOKI H,et al.Genetic dissection of pre-harvest sprouting resistance in an upland rice cultivar [J].Breeding Science Preview,2018,68(2):200-209.
[4] DONG Y J,T SUZUKI E,KAMIUNTEN H,et al.Identification of quantitative trait loci associated with preharvest sprouting resistance in rice (Oryza sativa L.) [J].Field Crops Res,2003,81(2/3):133-139.
[5] 卢丙越.水稻品种强休眠性的定位及遗传解析 [D].南京:南京农业大学,2011.
[6] 罗正良.水稻抗穗发芽主效QTL qPSR8的精细定位及候选基因分析 [D].雅安:四川农业大学,2012.
[7] DU L,XU F,FANG J,et al.Endosperm sugar accumulation caused by mutation of PHS8/ISA1 leads to pre-harvest sprouting in rice [J].Plant Journal,2018,95(3):545-556.
[8] TAKEUCHI Y,LIN S Y,SASAKI T,et al.Fine linkage mapping enables dissection of closely linked quantitative trait loci for seed dormancy and heading in rice [J].Theoretical and Applied Genetics,2003,107(7):1174-1180.
[9] GU X Y,KIANIAN S F,FOLEY M E.Multiple loci and epistases control genetic variation for seed dormancy in weedy rice (Oryza sativa) [J].Genetics,2004,166(3):1503-1516.
[10] SUGIMOTO K,TAKEUCHI Y,EBANA K,et al.Molecular cloning of Sdr4,a regulator involved in seed dormancy and domestication of rice [J].Proc Natl Acad Sci USA,2010,107(13):5792-5797.
[11] GU X Y,MICHAEL E F,DAVID P,et al.Association between seed dormancy and pericarp color is controlled by a pleiotropic gene that regulates abscisic acid and flavonoid synthesis in weedy red rice [J].Genetics,2011,189(4):1515-1524.
[12] YE H,FENG J H,ZHANG L H,et al.Map-based cloning of seed dormancy qSD1-2 indentified a gibberellin synthesis gene regulating the development of endosperm-im-posed dormancy in rice [J].Plant Physiology,2015,169(3):2152-2165.
[13] WANG M,LI W Z,FANG C,et al.Parallel selection on a dormancy gene during domestication of crops from multiple families [J].Nature Genetics,2018,50(10):1435-1441.
[14] POREBSKI S,BAILEY L G,BAUM B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components [J].Plant Mol Biol Rep,1997,15(1):8-15.
[15] SANGUINETTI C J,DIAS N E,SIMPSON A J.RAPD silver staining and recovery of PCR products separated on polyacrylamide gels [J].Biotechniques,1994,17(5):914-918.
[16] MENG L,LI H H,ZHNG L Y,et al.QTL IciMapping:Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations [J].Crop J,2015,3(3):269-283.
[17] LEE S J,OH C S,SUH J P,et al.Identification of QTLs for domestication-related and agronomic traits in an Oryza sativa × O.rufipogon BC1F7 population [J].Plant Breeding,2005,124(3):209-219.
[18] NAGAOKA L,SASAHARA H,TABUCHI H,et al.Improvement of seed shattering and dormancy in Oryza sativa L.‘Hokuriku 193’ based on genetic information [J].Breeding Science,2017,67(3):173-180.
[19] 陈海生,陶龙兴,王熹,等.水稻穗芽相关性状的QTL定位 [J].中国水稻科学,2006,20(3):253-258.
[20] ZHOU Y L,XIE Y H,CAI J L,et al.Substitution mapping of QTLs controlling seed dormancy using single segment substitution lines derived from multiple cultivated rice donors in seven cropping seasons [J].Theoretical and Applied Genetics,2017,130(6):1191-1205.
[21] GUO L B,ZHU L H,XU Y B,et al.QTL analysis of seed dormancy in rice (Oryza sativa L.) [J].Euphytica,2004,140(3):155-162.