MicroRNA-155在重度子痫前期患者胎盘中的表达及临床意义
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摘要
目的探讨microRNA-155在重度子痫前期患者胎盘中的表达及临床意义。方法选取在医院行剖宫产手术分娩的64例子痫前期孕妇作为子痫前期组,入院时间为2016年5月1日-2017年5月30日期间,将子痫前期分为轻度组(n=32)、重度组(n=32),选择同期在医院行剖宫产术分娩的32例健康孕妇作为择期剖宫产,采取实时荧光定量PCR技术(RT-PCR)检测对照组与子痫前期组孕妇胎盘组织中microRNA-155的表达水平。结果 microRNA-155在轻度、重度子痫前期组孕妇胎盘组织中的表达情况相比于对照组孕妇差异有统计学意义(P<0.05),其中重度子痫前期组micro RNA-155的表达较轻度组差异具有统计学意义(P<0.05);三组围生结局:子痫前期组的各亚组围生儿窒息率、产后出血率较对照组差异有统计学意义(P<0.05),其中以重度子痫前期组最高;围生儿死亡率,轻度子痫前期组与对照组比较差异无统计学意义,而重度子痫前期组的死亡率显著高于以上两组(P<0.05),且新生儿体重上,重度子痫前期组显著低于轻度组及对照组,分别为(2495.3±582.3)g、(3352.5±325.6)g、(3465.6±542.3)g,对照组与轻度组比较差异无统计学意义(P>0.05)。结论 microRNA-155可能参与重度子痫前期的发病机制。
Objective To investigate the expression and clinical significance of microRNA-155 in placenta of severe preeclampsia. Methods 64 cases of severe preeclampsia pregnant women in the caesarean section in our hospital were selected as sPE group,the time of admission was from May 2016 to May 2017,the preeclampsia was divided into mild group(n=32) and severe group(n=32).32 healthy pregnant women in the same period were treated with cesarean section as control group.Real time fluorescence quantitative PCR(RT-PCR) was used in the control group and the spE group in the placental tissue of pregnant women,the expression of microRNA-155 level was detected. Results The expression of micro RNA-155 in the placental tissue of pregnant women with mild preeclampsia and severe preeclampsia was significantly higher than that of the control group(P<0.05),and the expression of microRNA-155 in the severe preeclampsia group was significantly higher than that in the mild group,and the difference was statistically significant(P<0.05).The perinatal asphyxia rate,the postpartum hemorrhage rate and the mortality rate in the severe preeclampsia group were significantly higher than those in the mild group and the control group(P<0.05),and the weight of the newborn was significantly lower than that of the control group and the mild group(P<0.05). Conclusion Micro RNA-155 may be involved in the pathogenesis of severe preeclampsia.
Objective To investigate the expression and clinical significance of microRNA-155 in placenta of severe preeclampsia. Methods 64 cases of severe preeclampsia pregnant women in the caesarean section in our hospital were selected as sPE group,the time of admission was from May 2016 to May 2017,the preeclampsia was divided into mild group(n=32) and severe group(n=32).32 healthy pregnant women in the same period were treated with cesarean section as control group.Real time fluorescence quantitative PCR(RT-PCR) was used in the control group and the spE group in the placental tissue of pregnant women,the expression of microRNA-155 level was detected. Results The expression of micro RNA-155 in the placental tissue of pregnant women with mild preeclampsia and severe preeclampsia was significantly higher than that of the control group(P<0.05),and the expression of microRNA-155 in the severe preeclampsia group was significantly higher than that in the mild group,and the difference was statistically significant(P<0.05).The perinatal asphyxia rate,the postpartum hemorrhage rate and the mortality rate in the severe preeclampsia group were significantly higher than those in the mild group and the control group(P<0.05),and the weight of the newborn was significantly lower than that of the control group and the mild group(P<0.05). Conclusion Micro RNA-155 may be involved in the pathogenesis of severe preeclampsia.
引文
[1]李枫,姚丽,张喜红,等.miRNA-22在卵巢癌组织的表达及其对卵巢癌细胞增殖、迁移与侵袭的影响[J].中国病理生理杂志,2016,32(12):2251-2255.
[2]李伟,胡宝春,龚照,等.妊娠期糖尿病胎盘组织hsa-mi R95-5p、hsa-miR-548am-5p和hsa-miR-1246的表达及意义[J].实用医学杂志,2016,32(4):566-568,569.
[3]贾彦如,王丽红,郝丽兵,等.mi R-195及MAPK1在子痫前期患者胎盘组织中的表达[J].中华临床医师杂志(电子版),2016,10(24):3683-3688.
[4]许敏,李燕,李惠梅,等.子痫前期产妇胎盘组织miR-30家族miRNA及其下游基因表达变化[J].山东医药,2017,57(35):61-63.
[5]张展,王媛媛,李爱萍,等.子痫前期患者胎盘组织中miR-155及CXCR4的表达及意义[J].国际检验医学杂志,2017,38(9):1167-1171.
[6]俞晨,刘沈林,邹玺,等.灌肠方抑制晚期肠癌生长及对血清VEGF和mi RNA-31表达的临床研究[J].南京中医药大学学报,2014,30(1):33-36.
[7]霍雄伟,尚元春,魏光兵,等.胆囊癌组织中Cyr61和β-catenin的表达及临床意义[J].西安交通大学学报(医学版),2016,37(4):569-573.
[8]徐岩,蒋伟,马蕊,等.Cyr61过表达对缺血性急性肾损伤早期细胞凋亡的影响[J].中华肾脏病杂志,2016,32(7):513-518.
[9]周颖,汪燕,邱娜璇,等.早发型重度子痫前期合并胎儿生长受限期待治疗的妊娠结局探讨[J].实用妇产科杂志,2015,31(3):221-224.
[10]张展,王媛媛,李爱萍,等.子痫前期患者胎盘组织中miR-155及CXCR4的表达及意义[J].国际检验医学杂志,2017,38(9):1167-1171.
[2]李伟,胡宝春,龚照,等.妊娠期糖尿病胎盘组织hsa-mi R95-5p、hsa-miR-548am-5p和hsa-miR-1246的表达及意义[J].实用医学杂志,2016,32(4):566-568,569.
[3]贾彦如,王丽红,郝丽兵,等.mi R-195及MAPK1在子痫前期患者胎盘组织中的表达[J].中华临床医师杂志(电子版),2016,10(24):3683-3688.
[4]许敏,李燕,李惠梅,等.子痫前期产妇胎盘组织miR-30家族miRNA及其下游基因表达变化[J].山东医药,2017,57(35):61-63.
[5]张展,王媛媛,李爱萍,等.子痫前期患者胎盘组织中miR-155及CXCR4的表达及意义[J].国际检验医学杂志,2017,38(9):1167-1171.
[6]俞晨,刘沈林,邹玺,等.灌肠方抑制晚期肠癌生长及对血清VEGF和mi RNA-31表达的临床研究[J].南京中医药大学学报,2014,30(1):33-36.
[7]霍雄伟,尚元春,魏光兵,等.胆囊癌组织中Cyr61和β-catenin的表达及临床意义[J].西安交通大学学报(医学版),2016,37(4):569-573.
[8]徐岩,蒋伟,马蕊,等.Cyr61过表达对缺血性急性肾损伤早期细胞凋亡的影响[J].中华肾脏病杂志,2016,32(7):513-518.
[9]周颖,汪燕,邱娜璇,等.早发型重度子痫前期合并胎儿生长受限期待治疗的妊娠结局探讨[J].实用妇产科杂志,2015,31(3):221-224.
[10]张展,王媛媛,李爱萍,等.子痫前期患者胎盘组织中miR-155及CXCR4的表达及意义[J].国际检验医学杂志,2017,38(9):1167-1171.