日本红枫‘青龙’组织培养与快速繁殖
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摘要
以青龙半木质化枝条为外植体,采用丛生芽诱导途径,建立起组培快繁体系。结果表明:最佳启动培养基为WPM+0.05 mg/LTDZ+30 g/L蔗糖+6.5 g/L琼脂,启动率为96.1%;最佳增殖培养基为WPM+0.06 mg/L TDZ+0.3 mg/L IBA+6.5 g/L琼脂+30 g/L蔗糖,继代周期30 d,增殖系数高达4.8;最佳生根培养基为1/2WPM+0.3 mg/L NAA+6.5 g/L琼脂+30 g/L蔗糖,培养30 d,其生根率达96.7%。炼苗后,移栽到珍珠岩∶蛭石∶泥炭土体积比为1∶1∶1的混合基质中,成活率达到90%以上。
The explants were taken from the late and half lignificational stems with shoot in the adult selected trees. An economic and high effective technology system for rapid propagation of Acer palmatum ‘seiryu'was established, through multiple-shoots proliferation. The result show that the optimal starting medium was modified WPM + 0.05 mg/L TDZ+ 30 g/L sucrose + 6.5 g/L gelatin, the establishment rates were 96.1%; the optimal mulitiplication medium was modified WPM +0.06 mg/L TDZ+0.3 mg/L IBA + 30 g/L sucrose + 6.5 g/L gelatin, the subculture cycle was 30 d, the highest multiplication rate was 4.8; the optimal rooting medium was 1/2 WPM + 0.3 mg/L NAA + 30 g/L sucrose + 6.5 g/L gelatin, the rooting rate has reached 96.7% within 30 d. The transplanting matrix were pearlite,vermiculite and peat(1 : 1 : 1, V/V/V), and its corresponding transplanting survival rate was over 90%.
The explants were taken from the late and half lignificational stems with shoot in the adult selected trees. An economic and high effective technology system for rapid propagation of Acer palmatum ‘seiryu'was established, through multiple-shoots proliferation. The result show that the optimal starting medium was modified WPM + 0.05 mg/L TDZ+ 30 g/L sucrose + 6.5 g/L gelatin, the establishment rates were 96.1%; the optimal mulitiplication medium was modified WPM +0.06 mg/L TDZ+0.3 mg/L IBA + 30 g/L sucrose + 6.5 g/L gelatin, the subculture cycle was 30 d, the highest multiplication rate was 4.8; the optimal rooting medium was 1/2 WPM + 0.3 mg/L NAA + 30 g/L sucrose + 6.5 g/L gelatin, the rooting rate has reached 96.7% within 30 d. The transplanting matrix were pearlite,vermiculite and peat(1 : 1 : 1, V/V/V), and its corresponding transplanting survival rate was over 90%.
引文
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[12]王军辉,吴丽华,林娟.生长素对楸树不定芽的诱导和增殖培养影响的研究[J].林业科技,2011,36(1):1-4.
[13]张文泉,邓洁,罗国涛,等.青钱柳组织培养研究[J].中南林业科技大学学报,2018,38(3):13-19.
[2]陈正华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986:456-465.
[3]李佳莹,俞明亮,马瑞娟,等.三种桃砧木的离体快繁技术研究[J].江苏农业学报,2009,31(5):546-548.
[4]谈凯,方芳.日本红枫愈伤组织诱导分化的研究[J].安徽农学通报,2011,17(22):21-23.
[5]李艳菊,陶加洪,王兰珍,等.元宝枫组织培养研究[J].北京林业大学学报,2005,27(3):104-107.
[6]张桂和.麻竹茎尖培养及离体快繁研究[J].海南大学学报(自然科学版),1997,15(4):298-303.
[7]张铁,万京.勃氏甜龙竹的组培快繁[J].云南民族大学学报(自然科学版),2004,13(3):203-206.
[8]王关林,方宏筠,那杰.高活性细胞激动素TDZ在植物组织培养中的应用[J].植物学通报,1997(14):47-53.
[9]徐华松,徐九龙,黄学.TDZ在植物组织培养中的作用[J].广西植物,1996,16(1):77-80.
[10]王强,陈东怡,杨国,等.鳄嘴花的组织培养和快速繁殖[J].植物生理学报,2018,54(2):232-236.
[11]戴小英,刘新亮,邱凤英,等.黄樟茎段离体培养体系的建立[J].中南林业科技大学学报,2018,38(3):20-33.
[12]王军辉,吴丽华,林娟.生长素对楸树不定芽的诱导和增殖培养影响的研究[J].林业科技,2011,36(1):1-4.
[13]张文泉,邓洁,罗国涛,等.青钱柳组织培养研究[J].中南林业科技大学学报,2018,38(3):13-19.