小红细胞和RDW对Sysmex XE-5000血细胞分析仪血小板计数的影响
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摘要
目的探讨小红细胞和RDW对Sysmex XE-5000血细胞分析仪血小板计数的影响。方法选取中山大学孙逸仙纪念医院2018年3月至12月期间,住院患者标本240例,测定红细胞平均体积(MCV)和红细胞分布宽度(RDW),根据两者不同范围进行分组,记录PLT-I、PLT-O、RDW-CV三项指标的结果,并对所测标本用显微镜手工法进行血小板计数,最后通过SPSS19.0统计软件行配对t检验统计分析。结果 (1)当MCV为70~80fL、RDW-CV<15%时电阻抗法(PLT-I)与手工法计数血小板结果比较差异无统计学意义;MCV<60fL、MCV为60~69fL时电阻抗法(PLT-I)检测结果均高于手工法血小板计数,差异有统计学意义;MCV为70~80fL且RDW-CV≥15%时,电阻抗法(PLT-I)与手工法计数血小板的差异有统计学意义(P<0.01)。(2)当MCV<60fL、MCV为60fL~69fL、MCV为70fL~80fL时,血小板检测结果荧光法(PLT-O)均与手工法相近,差异无统计学意义(P>0.05)。结论小红细胞和RDW-CV大小对电阻抗法(PLT-I)影响较大,而荧光法(PLT-O)受二者影响较小,可以更准确反映血小板真实水平。
Objective To investigate the effect of small red blood cells and RDW on platelet count of Sysmex XE-5000 hematology analyzer. Methods A total of 240 inpatient specimens were selected from Sun Yat-sen Memorial Hospital of Sun Yat-sen University from March to December 2018. The mean red blood cell volume(MCV) and red blood cell distribution width(RDW) were determined and grouped according to the different ranges. PLT-I was recorded. The results of the three indicators, PLT-O, RDW-CV, and platelet counts were performed by microscope manual method. Finally, the paired t-test statistical analysis was performed by SPSS19.0 statistical software. Results(1) When the MCV was 70-80 fL and RDW-CV<15%, there was no significant difference between the electrical impedance method(PLT-I) and the manual counting platelet count. When MCV<60 fL and MCV were 60~69 fL The results of electrical impedance spectroscopy(PLT-I) were higher than those of manual platelet counts, and the difference was statistically significant. When MCV was 70-80 fL and RDW-CV ≥15%, electrical impedance method(PLT-I) and manual counting were used. Platelet differences were also statistically significant(P<0.01).(2) When MCV<60 fL, MCV was 60 fL~69 fL, MCV was 70 fL~80 fL, the platelet detection result fluorescence method(PLT-O) was similar to the manual method, and the difference was not statistically significant(P>0.05).Conclusion The size of small red blood cells and RDW-CV has a great influence on the electrical impedance method(PLT-I), while the fluorescence method(PLT-O) is less affected by both, which can more accurately reflect the true level of platelets.
Objective To investigate the effect of small red blood cells and RDW on platelet count of Sysmex XE-5000 hematology analyzer. Methods A total of 240 inpatient specimens were selected from Sun Yat-sen Memorial Hospital of Sun Yat-sen University from March to December 2018. The mean red blood cell volume(MCV) and red blood cell distribution width(RDW) were determined and grouped according to the different ranges. PLT-I was recorded. The results of the three indicators, PLT-O, RDW-CV, and platelet counts were performed by microscope manual method. Finally, the paired t-test statistical analysis was performed by SPSS19.0 statistical software. Results(1) When the MCV was 70-80 fL and RDW-CV<15%, there was no significant difference between the electrical impedance method(PLT-I) and the manual counting platelet count. When MCV<60 fL and MCV were 60~69 fL The results of electrical impedance spectroscopy(PLT-I) were higher than those of manual platelet counts, and the difference was statistically significant. When MCV was 70-80 fL and RDW-CV ≥15%, electrical impedance method(PLT-I) and manual counting were used. Platelet differences were also statistically significant(P<0.01).(2) When MCV<60 fL, MCV was 60 fL~69 fL, MCV was 70 fL~80 fL, the platelet detection result fluorescence method(PLT-O) was similar to the manual method, and the difference was not statistically significant(P>0.05).Conclusion The size of small red blood cells and RDW-CV has a great influence on the electrical impedance method(PLT-I), while the fluorescence method(PLT-O) is less affected by both, which can more accurately reflect the true level of platelets.
引文
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[2]许文艳,涂云贵,杜巧丽.血小板假性减少的原因分析与对策[J].国际检验医学与临床杂志,2016,37(8):1157-1161.
[3]叶长钦,罗辉,彭兰芬,等.小红细胞对电阻抗法测定血小板计数影响的分析[J].检验医学与临床,2010,7(6):539-540.
[4]丛玉隆,乐家新,袁家颖.实用血细胞分析技术与临床[M].北京:人民军医出版社,2011:304-306,375.
[5]黄晓军,沈志祥,王爱霞,等.血液病/恶性肿瘤患者侵袭性真菌感染的诊断标准与治疗原则(草案)[J].中华内科杂志,2005,44(7):554-556.
[6]周艺,陈春兰,冯文安,等.血细胞分析仪中MCV,RDW对PLT计数的影响的调查研究[J].中国卫生检验杂志,2011,21(7):1735-1739.