基于UFLC-IT-TOF/MS技术分析不同产地黄芪的化学成分
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摘要
为研究不同产地黄芪的化学成分差异,制备了甘肃和内蒙古2个产地共15批黄芪药材的水煎液,利用超快速液相色谱-离子阱飞行时间质谱仪(UFLC-IT-TOF/MS)采集15批黄芪药材水煎液化学成分的指纹图谱,进而运用偏最小二乘-判别分析(PLS-DA)和(非)参数检验对数据进行模式识别和统计分析,以变量投影重要性大于1、统计学差异小于0. 05以及倍数变化大于1. 5为阈值,筛选并鉴定其差异成分。结果发现,不同产地的黄芪水煎液中毛蕊异黄酮、芒柄花素、亚油酸等7个化学成分的含量有明显差异,涉及木脂素、黄酮、脂肪酸等4种类别。本研究为黄芪药材的全面质量控制、准确区分产地以及合理种植栽培提供了重要依据。
To uncover the differences in material basis of Astragalus membranaceus from different origins,ultrafast liquid chromatography-ion trap time-of-flight mass spectrometry( UFLC-IT-TOF/MS) was applied to characterize chemical features of 15 batches of water decoction of Astragalus membranaceus from Gansu Province and Inner Mongolia Autonomous Region. Pattern recognition and statistical analysis were performed using partial least squares-discriminant analysis( PLS-DA) and( non) parametric tests,respectively. The differential compounds with variable importance in projection( VIP) > 1,adjusted P < 0. 05,and fold change( FC) > 1. 5 were screened out and further identified. It was found that 7 different substances such as calycosin,formononetin and linoleic acid could be used as quality markers to distinguish the origin of Astragalus membranaceus. Our findings provided new potential quality markers for the accurate differentiation of geographical origins of Astragalus membranaceus as well as its comprehensive quality control.
To uncover the differences in material basis of Astragalus membranaceus from different origins,ultrafast liquid chromatography-ion trap time-of-flight mass spectrometry( UFLC-IT-TOF/MS) was applied to characterize chemical features of 15 batches of water decoction of Astragalus membranaceus from Gansu Province and Inner Mongolia Autonomous Region. Pattern recognition and statistical analysis were performed using partial least squares-discriminant analysis( PLS-DA) and( non) parametric tests,respectively. The differential compounds with variable importance in projection( VIP) > 1,adjusted P < 0. 05,and fold change( FC) > 1. 5 were screened out and further identified. It was found that 7 different substances such as calycosin,formononetin and linoleic acid could be used as quality markers to distinguish the origin of Astragalus membranaceus. Our findings provided new potential quality markers for the accurate differentiation of geographical origins of Astragalus membranaceus as well as its comprehensive quality control.
引文
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[18] Zhou R,Xu L,Ye M,et al. Formononetin inhibits migration andinvasion of MDA-MB-231 and 4T1 breast cancer cells bysuppressing MMP-2 and MMP-9 through PI3K/AKT signalingpathways[J]. Horm Metab Res,2014,46(11):753-760.
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[20] Yang B,Chen H,Stanton C,et al. Review of the roles of conjuga-ted linoleic acid in health and disease[J]. J Funct Foods,2015,15:314-325.
[21] Villacorta L,Minarrieta L,Salvatore SR,et al. In situ generation,metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation[J].Redox Bio,2018,15:522-531.
[22] Wang Y,Fan X,Qu H,et al. Strategies and techniques for multi-component drug design from medicinal herbs and traditionalChinese medicine[J]. Curr Top Med Chem,2012,12(12):1356-1362.
[23] Fu S,Gu Y,Jiang JQ,et al. Calycosin-7-O-β-d-glucoside regu-lates nitric oxide/caveolin-1/matrix metalloproteinases pathwayand protects blood-brain barrier integrity in experimental cerebralischemia-reperfusion injury[J]. J Ethnopharmacol,2014,155(1):692-701.
[2] Fu J,Wang Z,Huang L,et al. Review of the botanical character-istics,phytochemistry,and pharmacology of Astragalus membrana-ceus(Huangqi)[J]. Phytother Res,2014,28(9):1275-1283.
[3] Jin M,Zhao K,Huang Q,et al. Structural features and biologicalactivities of the polysaccharides from Astragalus membranaceus[J]. Int J Biol Macromol,2014,64:257-266.
[4] Li M,Wang W,Xue J,et al. Meta-analysis of the clinical value ofAstragalus membranaceus in diabetic nephropathy[J]. J Ethno-pharmacol,2011,133(2):412-419.
[5] Jiao J,Gai QY,Niu LL,et al. Enhanced production of two bioac-tive isoflavone aglycones in Astragalus membranaceus hairy rootcultures by combining deglycosylation and elicitation of immobi-lized edible Aspergillus niger[J]. J Agric Food Chem,2017,65(41):9078-9086.
[6] Shahzad M,Shabbir A,Wojcikowski K,et al. The antioxidanteffects of Radix Astragali(Astragalus membranaceus and relatedspecies)in protecting tissues from injury and disease[J]. CurrDrug Tar,2016,17(12):1331-1340.
[7] Liao XF,Bi XL,Xu AL,et al. Fingerprint and three constituents'quantitative determination for Astragali Radix from differentregions by HPLC-DAD-ELSD[J]. J Guangdong Pharm Univ(广东药科大学学报),2018,34(5):558-563.
[8] Tian D,Li ZY,Fan SC,et al. NMR-based analysis of water solu-ble extracts of different Astragali Radix[J]. Acta Pharm Sin(药学学报),2014,49(1):89-94.
[9] He JX,Mou QQ,Zhang JQ,et al. Rapid identification of AstragaliRadix from different origins by UPLC combined with chemomet-rics methods[J]. Chin Tradit Herbal Drugs(中草药),2017,48(1):179-184.
[10] Lai PK,Chan JY,Cheng L,et al. Isolation of anti-inflammatoryfractions and compounds from the root of Astragalus membrana-ceus[J]. Phytother Res,2013,27(4):581-587.
[11] Li J,Li F,Xu Y,et al. Chemical composition and synergisticantioxidant activities of essential oils from Atractylodes macroceph-ala and Astragalus membranaceus[J]. Nat Prod Commun,2013,8(9):1321-1324.
[12] Guan QX,Li CD. Screening test study on seedling pattern ofAstragalus membranaceus in different climate regions of Longxi(陇西不同气候区域黄芪育苗模式筛选试验研究)[J]. JChin Med Mate(中药材),2016,39(3):490-492.
[13] Liu DW,Gu CM,Yang QZ,et al. Resource surveys and suitabilityof origin for genuine medicinal materials,Astragalus membrana-ceus var. mongholicus in Inner Mongolia,China[J]. Chin J ApplEcol(应用生态学报),2016,27(3):838-844.
[14] Kai Z,Michela P,Antonio P and Annamaria P. Biological activeingredients of traditional Chinese herb Astragalus membranaceuson treatment of diabetes:a systematic review[J]. Mini-Rev MedChem,2015,15(4):315-329.
[15] Zhang LJ,Liu HK,Hsiao PC,et al. New isoflavonoid glycosidesand related constituents from astragali radix(Astragalus membra-naceus)and their inhibitory activity on nitric oxide production[J]. J Agric Food Chem,2011,59(4):1131-1137.
[16] Nie Y,Li S,Yi Y,et al. Effects of astragalus injection on theTGFβ/Smad pathway in the kidney in type 2 diabetic mice[J].BMC Complem Altern Med,2014,14(1):148.
[17] Li W,Sun YN,Yan XT,et al. Flavonoids from Astragalus mem-branaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendriticcells[J]. Arch Pharm Res,2014,37(2):186-192.
[18] Zhou R,Xu L,Ye M,et al. Formononetin inhibits migration andinvasion of MDA-MB-231 and 4T1 breast cancer cells bysuppressing MMP-2 and MMP-9 through PI3K/AKT signalingpathways[J]. Horm Metab Res,2014,46(11):753-760.
[19] Capozzi V,Menga V,Digesu AM,et al. Biotechnological produc-tion of vitamin B2-enriched bread and pasta[J]. J Agric FoodChem,2011,59(14):8013-8020.
[20] Yang B,Chen H,Stanton C,et al. Review of the roles of conjuga-ted linoleic acid in health and disease[J]. J Funct Foods,2015,15:314-325.
[21] Villacorta L,Minarrieta L,Salvatore SR,et al. In situ generation,metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation[J].Redox Bio,2018,15:522-531.
[22] Wang Y,Fan X,Qu H,et al. Strategies and techniques for multi-component drug design from medicinal herbs and traditionalChinese medicine[J]. Curr Top Med Chem,2012,12(12):1356-1362.
[23] Fu S,Gu Y,Jiang JQ,et al. Calycosin-7-O-β-d-glucoside regu-lates nitric oxide/caveolin-1/matrix metalloproteinases pathwayand protects blood-brain barrier integrity in experimental cerebralischemia-reperfusion injury[J]. J Ethnopharmacol,2014,155(1):692-701.