红景天苷对H_2O_2损伤后HT22细胞的保护作用
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摘要
目的以H_2O_2损伤的HT22细胞为体外氧化损伤模型,探讨红景天苷对HT22细胞氧化损伤的作用和机制。方法以0、250、500、1 000μmol/L H_2O_2损伤HT22细胞24 h后,用0、100、400μmol/L红景天苷干预24 h,在倒置显微镜下观察HT22细胞形态学变化。CCK-8比色法检测HT22细胞存活率。免疫荧光组织染色检测超氧化物歧化酶-2(SOD2)的表达。结果加入不同浓度H_2O_2诱导损伤后,HT22细胞生长被抑制,受抑制程度随H_2O_2浓度变化而渐变。H_2O_2损伤后,HT22细胞存活率呈浓度依赖性下降;红景天苷可以逆转HT22细胞存活率的下降,且使细胞形态变化得到改善。染色显示H_2O_2损伤后HT22细胞的SOD2表达增加,但红景天苷干预后SOD2的表达显著降低(P<0.05)。结论红景天苷可以拮抗H_2O_2氧化损伤发挥神经保护作用。
Objective To investigate the effect and mechanism of salidroside on oxidative injury of HT22 cells, and HT22 cells injured by H_2O_2 were used as oxidative injury model in vitro. Methods HT22 cells were injured by 0, 250, 500 and 1 000 μmol/L H_2O_2 for 24 hours, and then given 0, 100 and 400 μmol/L salidroside for 24 hours. The morphological changes of HT22 cells were observed under inverted microscope. CCK-8 colorimetric assay was used to detect the viability of HT22 cell. The expression of superoxide dismutase-2(SOD2) was detected by immunofluorescence staining. Results The growth of HT22 cells were inhibited by adding different concentration of H_2O_2, and the degree of inhibition changed gradually with the concentration of H_2O_2. After H_2O_2 injuryed, the survival rate of HT22 cells decreased in a concentrationdependent manner; salidroside could reverse the decline of HT22 cell survival rate and improve the morphological changes of HT22 cells. The staining showed that SOD2 expression in HT22 cells increased after H_2O_2 injuryed, but decreased significantly after salidroside intervention(P <0.05). Conclusion Salidroside can antagonize oxidative injury induced by H_2O_2 and exert neuroprotective effect.
Objective To investigate the effect and mechanism of salidroside on oxidative injury of HT22 cells, and HT22 cells injured by H_2O_2 were used as oxidative injury model in vitro. Methods HT22 cells were injured by 0, 250, 500 and 1 000 μmol/L H_2O_2 for 24 hours, and then given 0, 100 and 400 μmol/L salidroside for 24 hours. The morphological changes of HT22 cells were observed under inverted microscope. CCK-8 colorimetric assay was used to detect the viability of HT22 cell. The expression of superoxide dismutase-2(SOD2) was detected by immunofluorescence staining. Results The growth of HT22 cells were inhibited by adding different concentration of H_2O_2, and the degree of inhibition changed gradually with the concentration of H_2O_2. After H_2O_2 injuryed, the survival rate of HT22 cells decreased in a concentrationdependent manner; salidroside could reverse the decline of HT22 cell survival rate and improve the morphological changes of HT22 cells. The staining showed that SOD2 expression in HT22 cells increased after H_2O_2 injuryed, but decreased significantly after salidroside intervention(P <0.05). Conclusion Salidroside can antagonize oxidative injury induced by H_2O_2 and exert neuroprotective effect.
引文
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[4] QU ZQ,ZHOU Y,ZENG YS,et al.Pretreatment with Rhodiola rosea extract reduces cognitive impairment induced by intracerebroventricular streptozotocin in rats:implication of anti-oxidative and neuroprotective effects[J].Biomed Environ Sci,2009,22(4):318-326.
[5]邹毅清,蔡志扬,毛燕飞,等.红景天苷预处理对大鼠全脑缺血再灌注后神经行为学的影响[J].中西医结合学报,2009,7(2):130-134.
[6]张晓溪,郑晗,马战胜,等.红景天对睡眠剥夺大鼠学习记忆的影响及其机制探索[J].中国临床药理学与治疗学,2012,17(6):634-639.
[7]杨江河,魏敏,张蓉,等.红景天苷对癫痫大鼠认知功能障碍的治疗作用及其可能机制[J].现代生物医学进展,2011,11(15):2868-2871.