刺梨、桑叶、苦瓜饮料对糖尿病小鼠的降糖作用
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摘要
研究刺梨、桑叶、苦瓜饮料(配方饮料)对糖尿病小鼠的降糖效果。采用腹腔注射链脲佐菌素(STZ)构建Ⅰ型糖尿病小鼠模型,小鼠随机被分为模型组、阳性组、配方饮料组,并设空白组。模型组与空白组灌胃柠檬酸钠缓冲溶液,阳性组灌胃盐酸二甲双胍片,连续4周。期间测定小鼠的饮食饮水量、体质量、空腹血糖(FBG)、糖化血清蛋白(GSP)、糖化血红蛋白(GHb)、血清胰岛素(INS)、肝糖原以及脏器指数变化,并进行口服糖耐量试验(OGTT)。结果表明,该配方饮料能有效改善糖尿病小鼠多饮多食症状,抑制体质量下降,肝糖原含量极显著增加(P<0.01),FBG浓度、GSP浓度和GHb含量明显降低(P<0.01),血清胰岛素敏感性增强,表明配方饮料对糖尿病小鼠具有辅助降糖效果。
To study the hypoglycemic effect of Rosa roxburghii, Morus alba and Momordica charantia beverage(formula beverage) on diabetic mice,the typeⅠ diabetes mice model was constructed by intraperitoneal injection of streptozotocin(STZ), and mice were randomly divided into model group,positive group, recipe beverage group, and blank group. The model group and the blank group were given continuously gavage of sodium citrate buffer solution and the positive group was given continuously gavage of metformin hydrochloride tablets for 4 weeks. Changes in food intake and water intake, body mass, fasting blood glucose(FBG), glycosylated serum protein(GSP), glycosylated hemoglobin(GHb), serum insulin(INS), liver glycogen and organ indexes were measured during the period, and the oral glucose tolerance test(OGTT) was conducted. The results showed that the formula beverage effectively improved the polydipsia and polyphagia symptoms of diabetic mice, inhibit body mass loss, significantly increased liver glycogen content, significantly reduced FGB(P<0.01), GSP concentration and GHb relative content, and increased insulin sensitivity, indicating that the formula beverage had assistant hypoglycemic effect on diabetic mice.
To study the hypoglycemic effect of Rosa roxburghii, Morus alba and Momordica charantia beverage(formula beverage) on diabetic mice,the typeⅠ diabetes mice model was constructed by intraperitoneal injection of streptozotocin(STZ), and mice were randomly divided into model group,positive group, recipe beverage group, and blank group. The model group and the blank group were given continuously gavage of sodium citrate buffer solution and the positive group was given continuously gavage of metformin hydrochloride tablets for 4 weeks. Changes in food intake and water intake, body mass, fasting blood glucose(FBG), glycosylated serum protein(GSP), glycosylated hemoglobin(GHb), serum insulin(INS), liver glycogen and organ indexes were measured during the period, and the oral glucose tolerance test(OGTT) was conducted. The results showed that the formula beverage effectively improved the polydipsia and polyphagia symptoms of diabetic mice, inhibit body mass loss, significantly increased liver glycogen content, significantly reduced FGB(P<0.01), GSP concentration and GHb relative content, and increased insulin sensitivity, indicating that the formula beverage had assistant hypoglycemic effect on diabetic mice.
引文
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[3]PANG B,LIAN F,ZHAO X,et al.Prevention of type 2 diabetes with the traditional Chinese patent medicine:A systematic review and meta-analysis[J].Diabetes Res Clin Pr,2017,131:242-259.
[4]杨映霞,黄天敏,黄仁彬,等.杨桃果汁对糖尿病模型小鼠的降糖作用及其机制研究[J].广西医科大学学报,2019,36(1):7-10.
[5]张瑜,罗昱,刘芳舒,等.不同脱苦涩处理刺梨果汁风味品质分析[J].食品科学,2016,37(4):115-119.
[6]郭碧花.我国刺梨资源的开发利用现状及前景探讨[J].黑龙江科技信息,2016(20):65.
[7]谢勇,张榕,施伽,等.刺梨凝固型酸奶的发酵工艺研究[J].中国酿造,2017,36(10):181-185.
[8]张晓玲,瞿伟菁,孙斌,等.刺梨黄酮对实验性糖尿病的预防作用[J].营养学报,2004(6):474-476.
[9]常怡.苦瓜功能成分制备及降糖效果研究[D].长沙:湖南农业大学,2012.
[10]KUMAR S R,ASHISH J,SATISH N.Momordica charantia Linn.a mini review[J].Int J Biomed Res,2011,11(2):579-587.
[11]潘士佳.桑叶和苦瓜降糖成分的提取及药理活性考察[D].青岛:青岛科技大学,2011.
[12]聂凯.桑叶水提物对1型糖尿病小鼠治疗作用的研究[D].长沙:湖南农业大学,2012.
[13]张波.桑枝降糖活性成分及协同作用研究[D].重庆:西南大学,2013.
[14]吕侠影.苦瓜、苦荞、苦丁-复合果蔬汁茶饮料的研制及其贮藏稳定性研究[D].合肥:合肥工业大学,2015.
[15]提伟钢.蓝莓发酵乳饮料加工工艺研究[J].饮料工业,2015,18(3):33-36.
[16]BEGORRE M A,DIB A,HABCHI K,et al.Microvascular vasodilator properties of the angiotensin II type 2 receptor in a mouse model of typeⅠdiabetes[J].Sci Rep,2017,7:45625.
[17]史得君,严欢,崔清美,等.人参茎叶提取物对Ⅰ型糖尿病小鼠模型的影响[J].食品与机械,2017,33(7):165-169.
[18]张世奇.辣椒素对Ⅰ型糖尿病大鼠糖代谢的影响及其降糖机制的研究[D].重庆:西南大学,2017.
[19]沈吕春.龙胆泻肝散加减对肉鸡肝损伤的影响[D].广州:华南农业大学,2016.
[20]何华香.电针对PCPA致失眠大鼠脾脏TLR7信号通路的影响[D].广州:暨南大学,2015.