四黄止痢颗粒质量标准研究
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摘要
为了提高和完善《中华人民共和国兽药典》二○一○年版中四黄止痢颗粒的质量标准,改进四黄止痢颗粒项下黄连、黄柏的薄层鉴别方法,增加黄芩的定性鉴别。采用HPLC法测定黄芩苷含量。薄层色谱斑点清晰,阴性无干扰。黄芩苷对照品浓度在3.122~124.88μg/m L(r=0.9991,n=6)范围内与峰面积呈良好的线性关系;平均加样回收率为101.4%,RSD为1.6%。该方法简便、准确、重现性好、精密度高,可作为四黄止痢颗粒的质量控制方法。
To improve quality standard for Sihuangzhili Granules. Improve the TLC identification of coptidis rhizoma,cortex phellodendri for Sihuangzhili Granules in Edition 2010 of Chinese Veterinary Pharmacopoeia. Radix scutellariae was identified by TLC and baicalin was determined by HPLC. The TLC spots of these traditional chinese medicinal materials were fairly clear and the blank test showed no interference. The linear ranges were 3. 122 ~ 124.88 μg / m L for baicalin( r = 0. 9991,n = 6). The average recoveries rates were 101. 4% with RSD 1. 6%. The methods are available with a good reproducibility and can control the quality of Sihuangzhili Granules effectively.
To improve quality standard for Sihuangzhili Granules. Improve the TLC identification of coptidis rhizoma,cortex phellodendri for Sihuangzhili Granules in Edition 2010 of Chinese Veterinary Pharmacopoeia. Radix scutellariae was identified by TLC and baicalin was determined by HPLC. The TLC spots of these traditional chinese medicinal materials were fairly clear and the blank test showed no interference. The linear ranges were 3. 122 ~ 124.88 μg / m L for baicalin( r = 0. 9991,n = 6). The average recoveries rates were 101. 4% with RSD 1. 6%. The methods are available with a good reproducibility and can control the quality of Sihuangzhili Granules effectively.
引文
[1]中国兽药典委员会.中华人民共和国兽药典二○一○年版(二部)[S].
[2]中国兽药典委员会.中华人民共和国兽药典兽药使用指南中药卷二○一○年版(中药卷)[S].
[3]国家药典委员会.中华人民共和国药典二○一○年版(一部)[S].
[4]赵志军,王连水.小柴胡颗粒薄层鉴别及黄芩苷含量测定方法的研究[J].中国实验方剂学杂志,2005,(11):22-23.
[5]邸秀梅,肖秀玲,严绥平.黄芩药理学作用研究进展.内蒙古中医药,2007(4):57.
[6]文敏,李雪,付守廷.黄芩苷药理作用研究新进展[J].沈阳药科大学学报,2008,25(2):158.
[2]中国兽药典委员会.中华人民共和国兽药典兽药使用指南中药卷二○一○年版(中药卷)[S].
[3]国家药典委员会.中华人民共和国药典二○一○年版(一部)[S].
[4]赵志军,王连水.小柴胡颗粒薄层鉴别及黄芩苷含量测定方法的研究[J].中国实验方剂学杂志,2005,(11):22-23.
[5]邸秀梅,肖秀玲,严绥平.黄芩药理学作用研究进展.内蒙古中医药,2007(4):57.
[6]文敏,李雪,付守廷.黄芩苷药理作用研究新进展[J].沈阳药科大学学报,2008,25(2):158.