不同农作物用地磷细菌的分析及解磷能力的研究
摘要
通过选择性分离培养,研究了不同农作物用地的磷细菌特征,观察菌落形态特征,选择高效无机磷细菌和有机磷细菌;并利用透明圈法深入探讨了其解磷能力。结果显示:不同的农作物用地各理化参数有明显差异。无机磷含量:豌豆>生菜>番薯>茼蒿>笋>木耳菜;有机磷含量:生菜>木耳菜>番薯>笋>茼蒿>豌豆。从无机磷培养基分离的细菌总量看:笋>豌豆>茼蒿>木耳菜>番薯>生菜。其中番薯地中无机磷细菌数量最多(22×10~4ind./g),而木耳菜、生菜、茼蒿土壤中含少量磷细菌。选择6种优势菌种C1、D1、G1、H1、I1和J1进行解磷能力研究,发现其解磷能力:H1>G1>I1>J1>D1>C1。从有机磷细菌看:番薯>茼蒿>笋>生菜,豌豆和木耳菜未检出。选择3中优势菌种进行解磷能力分析,得出:F2>C2>B2。
引文
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[2]Peix A,Rivas R,Mateos P F,et al.Pseudomonas rhizosphaerae sp.nov.,a novel species that actively solubilizes phosphate in vitro[J].International Journal of Systematic and Evolutionary Microbiology,2003,53:2067-2072.
[3]Mehta S,Nautiyal C S.An efficient method for qualitative screening of phosphate solubilizing bacteria[J].Current Microbiology,2001,43:51-56.
[4]Tilak K V B R,Ranganayaki N,Pal K K,et al.Diversity of plant growth and soil health supporting bacteria[J].Current Science,2005,89(1):136-150.
[5]Abd-Alla M H.Phosphatases and the utilization of organic phosphorus by Rhizobium leguminosarum biovarviceae[J].Letters in Applied Microbiology,1994,18:294-296.
[6]Igual J M,Valverde A,Cervantes E,et al.Phosphate solubilizing bacteria as inoculants for agriculture:use of updated molecular techniques in their study[J].Agronomie,2001,21:561-568.
[7]于翠,吕德国,秦嗣军,等.本溪山樱根际与非根际解磷细菌群落结构及动态变化[J].应用生态学报,2006,17(12):2381-2384.
[8]朱培淼,杨兴明,徐阳春,等.高效解磷细菌的筛选及其对玉米苗期生长的促进作用[J].应用生态学报,2007,18(1):107-112.
[9]Rodríguez H,Fraga R.Phosphate solubilizing bacteria and their role in plant growth promotion[J].Biotechnol Adv,1999,17(4-5):319-339.
[10]Pérez E,Sulbarán M,Ball M M,et al.Isolation and characterization of mineral phosphate solubilizing bacteria naturally colonizing alimonitic crust in the south-eastern Venezuelan region[J].Soil Biology and Biochemistry,2007,39:2905-2914.
[11]葛城.微生物肥料的生产应用及发展[M].中国农业科技出版社,1996.
[12]席琳乔,瑞章.植物根际解磷菌的研究进展[J].塔里木大学学报,2006,18(4):57-61.
[13]王光华,赵英,周德瑞,等.解磷菌的研究现状与展望[J].生态环境,2003,l2(1):96-101.
[14]宋昭彬,何学福.饥饿对南方鲇仔稚鱼消化系统的形态和组织学影响[J].水生生物学报,2000,24(2):155-159.
[15]楼宝,史会采,骆季安,等.饥饿和再投喂对日本黄姑鱼代谢率和消化器官组织学的影响[J].2007,29(2):140-147.
[16]环境保护部科技标准司组织.碱熔-钼锑抗分光光度法[S].环境保护部,2012,1(3):HJ 632-2011.
[17]沈志群,张琪,刘琳娟,邱燕.碳酸氢钠浸提-钼锑抗分光光度法测定土壤中的有效磷[J].监测技术,2011,10:1674-6732.
[18]李山,刘丹.磷钼蓝吸光光度法测定环境土样中无机磷[J].理化检验:化学分册,2006,35(1):40-42.
[19]于天仁,王振权.土壤分析化学[M].北京:科学出版社,1988:12-13.
[20]邢素芝,郭含莲,汪建飞,等.麦田土壤解无机磷细菌的分离、筛选及其解磷效果[J].生物学杂志,2010,10(5):111-115.
[21]郑世仲,江胜滔,黄燕翔,沈嘉彬.土壤中有机解磷细菌的分离筛选鉴定[J].安徽农学通报,2009,15(15):66-70.