牙周炎大鼠正畸牙移动过程中基质金属蛋白酶7在牙周组织中的表达
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摘要
背景:基质金属蛋白酶7主要功能是降解Ⅳ型胶原、弹力蛋白、蛋白聚糖以及纤维粘连蛋白,研究提示其可能参与了牙周炎的组织破坏过程。目的:观察牙周炎大鼠在正畸力刺激下基质金属蛋白酶7在牙周组织中的表达。方法:实验选用60只8周龄左右的健康雄性SD大鼠,随机分为加力对照组和牙周炎牙移动组,建立牙周炎牙移动动物模型,分别在加力0,1,3,7,14,21 d处死大鼠,采用苏木精-伊红染色和免疫组织化学法研究牙周炎大鼠牙齿受力的不同阶段牙周组织中基质金属蛋白酶7表达和分布变化。结果与结论:(1)基质金属蛋白酶7阳性表达主要定位于上皮细胞和巨噬细胞的胞浆中,呈棕黄色。基质金属蛋白酶7在正常组织有表达,随着加力时间的延长,基质金属蛋白酶7阳性细胞数目逐渐增多,加力对照组和牙周炎加力组大鼠牙周组织内基质金属蛋白酶7的表达均在受力后7 d达到高峰,之后开始下降;(2)0-14 d牙周炎加力组大鼠张力侧、压力侧牙周组织中基质金属蛋白酶7的表达均显著高于加力对照组(P <0.05);3,7 d加力对照组张力侧基质金属蛋白酶7的表达显著高于压力侧(P <0.05);3,7 d牙周炎加力组张力侧基质金属蛋白酶7的表达显著高于压力侧(P<0.05);(3)结果表明,基质金属蛋白酶7作为牙周组织中的局部调控因子参与了正畸牙周组织的改建过程,正畸力和牙周炎均可导致基质金属蛋白酶7的表达增高。
BACKGROUND: Matrix metalloproteinase 7 has abilities of degrading collagen type Ⅳ, elastin, proteoglycan and fibronectin, which has been shown to be highly associated with periodontitis in gingival tissue biopsies.OBJECTⅣE: To investigate the expression of matrix metalloproteinase 7 in the periodontium of rats under orthodontic force. METHODS: Sixty healthy male Sprague-Dawley rats aged 8 weeks were randomly divided into orthodontic force and tooth movement groups. The animal models of orthodontic tooth movement were established. The rats were sacrificed at 0, 1, 3, 7, 14 and 21 days after tooth movement, respectively. The expression and distribution of Matrix metalloproteinase 7 in the periodontium were detected by hematoxylin-eosin staining and immunohistochemistry. RESULTS AND CONCLUSION: Matrix metalloproteinase 7 was mainly expressed in the cytoplasm of epithelial cells and macrophages, which appeared to be yellow. Matrix metalloproteinase 7 was visible in the normal tissue, and the number of cells positive for matrix metalloproteinase 7 increased with time, which peaked on day 7 after orthodontic force and then began to decrease. The expression levels of matrix metalloproteinase 7 in the periodontium of tension and pressure sides in the periodontitis tooth movement group were significantly higher than those in the control group at 0-14 days(P < 0.05). At 3 and 7 days after orthodontic force, the expression level of matrix metalloproteinase 7 in the periodontium of tension side in both groups was significantly higher than that of the pressure side(both P < 0.05). These findings show that Matrix metalloproteinase 7, as a local regulator in the periodontium, participates in the orthodontic periodontal tissue remodeling. Orthodontic force and inflammatory stimulus can up-regulate the expression of matrix metalloproteinase 7.
BACKGROUND: Matrix metalloproteinase 7 has abilities of degrading collagen type Ⅳ, elastin, proteoglycan and fibronectin, which has been shown to be highly associated with periodontitis in gingival tissue biopsies.OBJECTⅣE: To investigate the expression of matrix metalloproteinase 7 in the periodontium of rats under orthodontic force. METHODS: Sixty healthy male Sprague-Dawley rats aged 8 weeks were randomly divided into orthodontic force and tooth movement groups. The animal models of orthodontic tooth movement were established. The rats were sacrificed at 0, 1, 3, 7, 14 and 21 days after tooth movement, respectively. The expression and distribution of Matrix metalloproteinase 7 in the periodontium were detected by hematoxylin-eosin staining and immunohistochemistry. RESULTS AND CONCLUSION: Matrix metalloproteinase 7 was mainly expressed in the cytoplasm of epithelial cells and macrophages, which appeared to be yellow. Matrix metalloproteinase 7 was visible in the normal tissue, and the number of cells positive for matrix metalloproteinase 7 increased with time, which peaked on day 7 after orthodontic force and then began to decrease. The expression levels of matrix metalloproteinase 7 in the periodontium of tension and pressure sides in the periodontitis tooth movement group were significantly higher than those in the control group at 0-14 days(P < 0.05). At 3 and 7 days after orthodontic force, the expression level of matrix metalloproteinase 7 in the periodontium of tension side in both groups was significantly higher than that of the pressure side(both P < 0.05). These findings show that Matrix metalloproteinase 7, as a local regulator in the periodontium, participates in the orthodontic periodontal tissue remodeling. Orthodontic force and inflammatory stimulus can up-regulate the expression of matrix metalloproteinase 7.
引文
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[6]李峰,孙晓菊,谢洪,等.牙周炎患者血清MMP-9、MMP-7以及MCP-1水平与颈动脉斑块不稳定性的相关性研究[J].上海口腔医学,2015,24(5):589-593.
[7]Han J,Xu X,Zhang B,et al.Expression of ATF4 and RUNX2 in periodontal tissue of pressure side during orthodontic tooth movement in rat.Int J Clin Exp Med.2015;8(1):934-838.
[8]Boas N,Andressa V,Chaves de S,et al.Orthodontic Force Increases Interleukin-1 beta and Tumor Necrosis Factor-alpha Expression and Alveolar Bone Loss in Periodontitis.J Periodontol.2013;84(9):1319-1326.
[9]Kittaka M,Shib H,Kajiya M,et al.Anti Microbial peptideLL37promotes vascular endothelial growth factor-A expression in humanperiodontal ligament cells.JPR.2013;48(2):228-234.
[10]Cossetin E,Janson G,de Carvalho MG,et al.Influence of low-level laser on bone remodeling during induced tooth movement in rats.Angle Orthod.2013;83(6):1015-1021.
[11]Hou J,Chen Y,Meng X,et al.Compressive force regulates ephrinB2 and EphB4 in osteoblasts and osteoclasts contributing to alveolar bone resorption during experimental tooth movement.Korean J Orthod.2014;44(6):320-329.
[12]袁建,初秀红,陈晖,等.MMPs在口腔疾病中的作用[J].临床口腔医学杂志,2011,27(11):698-700.
[13]孙晓菊,左海燕,谢洪,等.口腔鳞状细胞癌中PTEN、MMP-7和VEGF的表达及相关性研究[J].中国实用口腔科杂志,2013,3(3):174-176.
[2]Han J,Xu X,Zhang B,et al.Expression of ATF4 and RUNX2 in periodontal tissue of pressure side duringorthodontic tooth movement in rat.Int J Clin Exp Med.2015;8(1):934-838.
[3]Lv S,Li J,Feng W,et al.Expression of HMGB1 in the periodontal tissue subjected to orthodontic force application by Waldo's method in mice.J Mol Histo.2015;46(1):107-114.
[4]Hou J,Chen Y,Meng X.et al.Compressive force regulates ephrinB2and EphB4 in osteoblasts and osteoclasts contributing to alveolar bone resorption during experimental tooth movement[J].Korean JOrthod.2014;44(6):320-329.
[5]陈琦,赵刚,王晓艳,等.人龈沟液中MMP-2及TIMP-2在牙移动过程中表达的研究[J].口腔医学,2015,35(1):58-60.
[6]李峰,孙晓菊,谢洪,等.牙周炎患者血清MMP-9、MMP-7以及MCP-1水平与颈动脉斑块不稳定性的相关性研究[J].上海口腔医学,2015,24(5):589-593.
[7]Han J,Xu X,Zhang B,et al.Expression of ATF4 and RUNX2 in periodontal tissue of pressure side during orthodontic tooth movement in rat.Int J Clin Exp Med.2015;8(1):934-838.
[8]Boas N,Andressa V,Chaves de S,et al.Orthodontic Force Increases Interleukin-1 beta and Tumor Necrosis Factor-alpha Expression and Alveolar Bone Loss in Periodontitis.J Periodontol.2013;84(9):1319-1326.
[9]Kittaka M,Shib H,Kajiya M,et al.Anti Microbial peptideLL37promotes vascular endothelial growth factor-A expression in humanperiodontal ligament cells.JPR.2013;48(2):228-234.
[10]Cossetin E,Janson G,de Carvalho MG,et al.Influence of low-level laser on bone remodeling during induced tooth movement in rats.Angle Orthod.2013;83(6):1015-1021.
[11]Hou J,Chen Y,Meng X,et al.Compressive force regulates ephrinB2 and EphB4 in osteoblasts and osteoclasts contributing to alveolar bone resorption during experimental tooth movement.Korean J Orthod.2014;44(6):320-329.
[12]袁建,初秀红,陈晖,等.MMPs在口腔疾病中的作用[J].临床口腔医学杂志,2011,27(11):698-700.
[13]孙晓菊,左海燕,谢洪,等.口腔鳞状细胞癌中PTEN、MMP-7和VEGF的表达及相关性研究[J].中国实用口腔科杂志,2013,3(3):174-176.