委陵菜属几种植物总黄酮的提取
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摘要
许多蔷薇科(Rosaceae)委陵菜属(Potentilla L.)植物由于其含有多种次生代谢物,尤其是黄酮类物质,具有非常高的药用价值和经济价值.采用超声波震荡方法提取委陵菜属4种植物的总黄酮,通过单因素实验,确定了料液混和物质量浓度、乙醇含量、提取温度和提取时间这4个影响植物总黄酮提取的主要因素.在此基础上,设计了4因素3水平的正交试验,最终得到提取4种植物黄酮的最优方案.委陵菜在70℃,料液混合物10.0mg/mL,600mL/L乙醇,提取60min时效果最佳;皱叶委陵菜在70℃,料液混合物10.0mg/mL,700mL/L乙醇,提取50min时效果最佳;西山委陵菜在70℃,料液混合物10.0mg/mL,600mL/L乙醇,提取70min时效果最佳;翻白草在50℃,料液混合物10.0mg/mL,600mL/L乙醇,提取60min时效果最佳.这4种委陵菜属植物在它们各自相对应的提取最佳方案下得到的黄酮依次为13.126,34.712,36.482,45.973mg/g.实验流程简单,成本较低,毒性较小,适合于工业化的推广和应用.
Many plants in Rose family(Rosaceae)Potentillagenus(Potentilla L.)have high medicinal and economic value since they contain a variety of secondary metabolites,especially flavonoids.Single factor experiment was performed to determine the effect of four factors(including solid-liquid mass concentration,ethanol content,temperature and time)on the extraction of flavonoids from plants in Potentilla by using ultrasonic extraction.The conditions of flavonoids extraction from four plants were studied by using a 4-factor 3-level orthogonal test.For Potentilla,the best extraction effect was obtained under 70℃,using10.0 mg/mL solid-liquid and 600 mL/L ethanol,with 60 minutes' extraction.For P.ancistrifolia,the condition for best extraction effect was 70 ℃,using 10.0 mg/mL solid-liquid and 700 mL/L ethanol,with 50 minutes' extraction.For P.sischanensis,the optimal extraction condition was 70℃,using 10.0 mg/mL solid-liquid and 600 mL/L ethanol,with 70 minutes' extraction.For P.discolor,the optimal extraction condition was 50 ℃,using 10.0 mg/mL solid-liquid and 600 mL/L ethanol,with 60 minutes' extraction.The contents of total flavonoids extracted from the four plants under their optimal extraction conditions were13.126,34.712,36.482,45.973 mg/g,respectively.The extraction procedure that has been set up in this study is simple,cost-effective,less toxic,and is suitable for industrial application.
Many plants in Rose family(Rosaceae)Potentillagenus(Potentilla L.)have high medicinal and economic value since they contain a variety of secondary metabolites,especially flavonoids.Single factor experiment was performed to determine the effect of four factors(including solid-liquid mass concentration,ethanol content,temperature and time)on the extraction of flavonoids from plants in Potentilla by using ultrasonic extraction.The conditions of flavonoids extraction from four plants were studied by using a 4-factor 3-level orthogonal test.For Potentilla,the best extraction effect was obtained under 70℃,using10.0 mg/mL solid-liquid and 600 mL/L ethanol,with 60 minutes' extraction.For P.ancistrifolia,the condition for best extraction effect was 70 ℃,using 10.0 mg/mL solid-liquid and 700 mL/L ethanol,with 50 minutes' extraction.For P.sischanensis,the optimal extraction condition was 70℃,using 10.0 mg/mL solid-liquid and 600 mL/L ethanol,with 70 minutes' extraction.For P.discolor,the optimal extraction condition was 50 ℃,using 10.0 mg/mL solid-liquid and 600 mL/L ethanol,with 60 minutes' extraction.The contents of total flavonoids extracted from the four plants under their optimal extraction conditions were13.126,34.712,36.482,45.973 mg/g,respectively.The extraction procedure that has been set up in this study is simple,cost-effective,less toxic,and is suitable for industrial application.
引文
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[16] RAMOS S.Effects of Dietary Flavonoids Apoptotic Pathways Related to Cancer Chemoprevention[J].Nutritional Biochemistry,2007,18(7):427-442.
[17] SHIAN Y,FUNABASHI N,LEE K,et al.Acute Effect of Oral Flavonoid-rich Dark Chocolate Intake on Coronary Circulation,as Compared with Non-flavonoid White Chocolate,by Transthoracic Doppler Echocardiography in Healthy adults[J].International Journal of Cardiology,2009,131(3):424-429.doi:10.1016/j.ijcard.2007.07.131
[18]陈乃富,张莉.金樱子黄酮类化合物的初步研究[J].中国林副特产,2005(5):2-4.
[19]李贞.委陵菜对四氯化碳致小鼠肝损伤保护作用[J].辽宁中医杂志,2004,31(5):422-423.
[20]许正刚,闽运江.安徽产委陵菜属7种药用种类的黄酮提取与分析[J].安徽农业科学,2010,38(6):2929-2933.
[21]邹俊利.翻白草的研究进展[J].黑龙江医药科学,2006,29(4):104-104.
[22]明道绪.生物统计附实验设计[M].北京:中国农业出版社,2005:309-314.
[23]张德权,台建祥.生物类黄酮研究及应用概况[J].食品与发酵工业,1999,25(6):5256.
[24]邱贺媛,章宋洁.薄荷叶中黄酮类物质的提取及其抗氧化活性研究[J].安徽农业科学,2010,38(34):19335-19337,19340.
[2]刘天慰.山西植物志[M].北京:中国科学技术出版社,1998:286.
[3]《中华草本》编委会.中华草本[M].上海:上海科学技术出版社,2000:1024.
[4]全国中草药汇编编写组.全国中草药汇编[M].北京:人民卫生出版社,1975.
[5]任燕利,曲玮,梁敬钰.委陵菜属植物研究进展[J].海峡药学,2010,22(5):1-8.
[6]沈阳,王庆贺,林厚文,等.委陵菜化学成分的研究[J].中药材,2006,29(3):237-239.
[7]张萍.勿将委陵菜混作翻白草[J].中国中药杂志,2004,29(8):811-812.
[8] WANG J Q,LI J,ZOU Y H.Preventive Effects of Total Flavonoids of Litsea Coreana Level on Hepatic Steatosis in Rat Fed with High Fat Diet[J].Ethnopharmacology,2008,121(1):54-60.doi:10.1016/j.jep.2008.09.029
[9]陈乃富.蕨类黄酮类化合物的提取及其抗氧化作用[J].食品与发酵工业,2003,29(11):63-66.
[10]裴凌鹏,李文州,唐粉芳.葛根总黄酮成分的超声提取及抗氧化作用[J].北京联合大学学报(自然科学版),2003(3):25-27.doi:10.16255/j.cnki.ldxbz.2003.03.006
[11] ZHANG Y Y,WANG X Y.Protective Effect of Flavonoids from Scutellaria Baicalensis Georgi on Cerebral Ischemia Injury[J].Ethnopharmacology,2006,108(3):355-360.doi:10.1016/j.jep.2006.05.022
[12] PETER C H H.Analysis and Health Effects of Flavonoids[J].Food Chemisty,1996,57(1):43-46.
[13]黄锁义,覃成箭,姚小敏,等.洋葱总黄酮的提取及鉴定[J].时珍国医国药,2006,17(2):224-225.
[14] VISWANATH B G.Effects of Flavonoids-rich Extract from Seeds of Eugenia Jambolana L on Carbohydrate and Lipid Metabolism in Diabetic Mice[J].Food Chemistry,2008,110(3):697-705.doi:org/10.1016/j.foodchem.2008.02.068
[15]黄骅艺,查锡良.黄酮类化合物抗肿瘤作用研究进展[J].中国新药与临床杂志,2002,21(7):428-433.doi:10.1016/j.jnutbio.2006.11.004
[16] RAMOS S.Effects of Dietary Flavonoids Apoptotic Pathways Related to Cancer Chemoprevention[J].Nutritional Biochemistry,2007,18(7):427-442.
[17] SHIAN Y,FUNABASHI N,LEE K,et al.Acute Effect of Oral Flavonoid-rich Dark Chocolate Intake on Coronary Circulation,as Compared with Non-flavonoid White Chocolate,by Transthoracic Doppler Echocardiography in Healthy adults[J].International Journal of Cardiology,2009,131(3):424-429.doi:10.1016/j.ijcard.2007.07.131
[18]陈乃富,张莉.金樱子黄酮类化合物的初步研究[J].中国林副特产,2005(5):2-4.
[19]李贞.委陵菜对四氯化碳致小鼠肝损伤保护作用[J].辽宁中医杂志,2004,31(5):422-423.
[20]许正刚,闽运江.安徽产委陵菜属7种药用种类的黄酮提取与分析[J].安徽农业科学,2010,38(6):2929-2933.
[21]邹俊利.翻白草的研究进展[J].黑龙江医药科学,2006,29(4):104-104.
[22]明道绪.生物统计附实验设计[M].北京:中国农业出版社,2005:309-314.
[23]张德权,台建祥.生物类黄酮研究及应用概况[J].食品与发酵工业,1999,25(6):5256.
[24]邱贺媛,章宋洁.薄荷叶中黄酮类物质的提取及其抗氧化活性研究[J].安徽农业科学,2010,38(34):19335-19337,19340.