当归挥发油对人肺腺癌GLC-82细胞增殖及细胞周期的影响
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摘要
目的:探讨当归挥发油对人肺腺癌GLC-82细胞增殖及细胞周期的作用。方法:采用噻唑蓝(MTT)比色法检测当归挥发油对GLC-82细胞存活率的影响;倒置显微镜下观察细胞生长形态;流式细胞仪检测当归挥发油对GLC-82细胞周期的影响。结果:在6. 25～200μg/m L浓度范围内当归挥发油对GLC-82细胞增殖具有浓度依赖性抑制作用,当归挥发油对GLC-82细胞的I C50为113. 03μg/mL;倒置显微镜下观察发现阴性组细胞具有GLC-82细胞典型的形态特征,多边形,成片生长,随着当归挥发油浓度的升高,细胞数量减少,散在生长,形态发生变化,逐渐皱缩;经当归挥发油处理后GLC-82细胞表现出明显的G2期周期阻滞。结论:当归挥发油可抑制GLC-82细胞生长,引起细胞周期G2期的阻滞。
Objective: To discuss the effects of volatile oil from DangGui(Angelica sinensis) on cell cycle and the proliferation of human lung adenocarcinoma GLC-82 cells. Methods: The effects of volatile oil from DangGui on survival rate of GLC-82 cells were detected by using MTT chromometry; cell growth morphology was observed under the inverted microscope; the influence of GLC-82 cell cycle was measures by flow cytometry. Results: Volatile oil from DangGui inhibited the proliferation of GLC-82 cells in concentration-dependent manner ranged between 6.25~200 μg/mL, IC50 of volatile oil from DangGui to GLC-82 cells was 113.03 μg/m L; the results under the inverted microscope showed that the cells in negative group presented typical morphological features of GLC-82 cells, polygon, flaky growth, as the concentrations of volatile oil from DangGui raised, cellular numbers reduced,spread in growth, the shape has changed and shrunk gradually; the results of flow cytometry indicated that GLC-82 cells performed the obvious cell cycle arrest at phase G2 after dealing with volatile oil from DangGui. Conclusion:Volatile oil from DangGui could inhibit the growth of GLC-82 cells and cause cell cycle arrest at phase G2.
Objective: To discuss the effects of volatile oil from DangGui(Angelica sinensis) on cell cycle and the proliferation of human lung adenocarcinoma GLC-82 cells. Methods: The effects of volatile oil from DangGui on survival rate of GLC-82 cells were detected by using MTT chromometry; cell growth morphology was observed under the inverted microscope; the influence of GLC-82 cell cycle was measures by flow cytometry. Results: Volatile oil from DangGui inhibited the proliferation of GLC-82 cells in concentration-dependent manner ranged between 6.25~200 μg/mL, IC50 of volatile oil from DangGui to GLC-82 cells was 113.03 μg/m L; the results under the inverted microscope showed that the cells in negative group presented typical morphological features of GLC-82 cells, polygon, flaky growth, as the concentrations of volatile oil from DangGui raised, cellular numbers reduced,spread in growth, the shape has changed and shrunk gradually; the results of flow cytometry indicated that GLC-82 cells performed the obvious cell cycle arrest at phase G2 after dealing with volatile oil from DangGui. Conclusion:Volatile oil from DangGui could inhibit the growth of GLC-82 cells and cause cell cycle arrest at phase G2.
引文
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[5]张景屹,杜红娣.肺癌化疗患者癌因性疲乏状况调查分析[J].中国医药导报,2012,9(15):24-26.
[6]郑秋青,毛伟敏,张沂平,等.肺癌早期诊断及个体化治疗研究[J].中国肿瘤,2013,22(10):784-788.
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[8]张艳霞,缪苗苗,吴勇杰,等.蛇葡萄素钠协同卡铂体外抑制人肺腺癌GLC-82细胞增殖的作用[J].中药药理与临床,2014,30(6):38-41.
[9]李妍,韩锋锋.肺癌中药治疗的研究进展[J].世界临床药物,2016,37(7):503-504.
[10]Xie J,Liu J,Liu H,et al.The antitumor effect of tanshinoneⅡA on anti-proliferation and decreasing VEGF/VEGFR2 expression on the human non-small cell lung cancer A549 cell line[J].Acta Pharm Sin B,2015,5(6):554-563.
[11]王家晓,张玉梅.人参皂甙Rg3对肺癌荷瘤小鼠免疫功能影响的研究[J].广西医学,2013,35(1):13-15.
[12]李佩文.中西医临床肿瘤学[M].北京:中国协和医科大学出版社,1996:101-113.
[13]张伟伟,陆茵.丹参抗肿瘤活性成分研究新进展[J].中国中药杂志,2010,35(3):389-392.
[14]王君明,崔瑛.山豆根化学成分、药理作用及毒性研究进展[J].中国实验方剂学杂志,2011,17(4):229-232.
[15]高默杰,徐忠伟,王凤梅,等.钠钾泵抑制剂通过调节细胞周期相关蛋白的生成介导肝癌HepG2细胞周期S期阻滞与凋亡[J].中国药理学通报,2010,26(4):452-456.