常用生物农药对草地贪夜蛾的室内防效
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摘要
世界重大迁飞害虫草地贪夜蛾已在中国中南部地区发生为害,对中国农作物生产构成了严重威胁。目前,生产上主要依赖化学农药控制其种群发展,这很可能导致草地贪夜蛾产生抗药性并对农业生态系统造成负面影响。为了减少化学农药的用量,我们在室内测试了10种常用抗生素、微生物和植物源生物农药对草地贪夜蛾的防治效果。结果表明,各种生物农药对草地贪夜蛾的杀卵活性均低于25%,但对幼虫有不同程度的控制作用。用药5 d后,抗生素类杀虫剂25 g/L多杀霉素ME、10%多杀霉素WG和1.5%阿维菌素UL对2龄幼虫的校正防效分别为82.67%、76.00%和54.67%;微生物杀虫剂80亿孢子/g球孢白僵菌OF和8 000 IU/μL苏云金杆菌SC对2龄幼虫的校正死亡率为60%～71.62%;植物源生物农药0.3%苦参碱AS、0.3%印楝素EC和6%鱼藤酮ME对2龄幼虫防效较差,校正死亡率均低于42%。结果表明,多杀霉素、阿维菌素、苏云金芽孢杆菌与球孢白僵菌可用于防治田间草地贪夜蛾。
The fall armyworm, Spodoptera frugiperda(J. E. Smith, 1797) is an important migratory pest for crop production worldwide. It has been recently recorded in southern and central China in early May 2019 and produced a great threat to crop production. Nowadays, chemical insecticides are widely used to manage its population development, which would lead to insecticides resistance of pest insects and adverse effect on agricultural ecosystem. To reduce the use of chemical insecticides, the experiment was carried out to assess the efficacy of 10 commonly used biopesticides e.g. antibiotic, microbial and botanical extracts to control S. frugiperda. Our results showed that all examined biopesticides did not inhibit the hatch of eggs well, while their control efficacies on larvae were quite different. The antibiotic biopesticides spinosad 25 g/L ME, spinosad 10% WG and abamectin 1.5% UL, caused a high mortality of 82.67%, 76.00% and 54.67%, respectively, to the 2 nd instar larvae, while the microbial biopesticides Beauveria bassiana 8 billion spores/g OF and Bacillus thuringiensis 8 000 IU/μL SC caused a mortality of 60%-71.62%. However, the botanical biopesticides matrine 0.3% AS, azadirachtin 0.3% EC and rotenone 6% ME caused a much lower mortalities(<42%). In conclusion, the spinosad, abamectin, Bacillus thuringiensis and Beauveria bassiana are recommended for the control of field S. frugiperda populations.
The fall armyworm, Spodoptera frugiperda(J. E. Smith, 1797) is an important migratory pest for crop production worldwide. It has been recently recorded in southern and central China in early May 2019 and produced a great threat to crop production. Nowadays, chemical insecticides are widely used to manage its population development, which would lead to insecticides resistance of pest insects and adverse effect on agricultural ecosystem. To reduce the use of chemical insecticides, the experiment was carried out to assess the efficacy of 10 commonly used biopesticides e.g. antibiotic, microbial and botanical extracts to control S. frugiperda. Our results showed that all examined biopesticides did not inhibit the hatch of eggs well, while their control efficacies on larvae were quite different. The antibiotic biopesticides spinosad 25 g/L ME, spinosad 10% WG and abamectin 1.5% UL, caused a high mortality of 82.67%, 76.00% and 54.67%, respectively, to the 2 nd instar larvae, while the microbial biopesticides Beauveria bassiana 8 billion spores/g OF and Bacillus thuringiensis 8 000 IU/μL SC caused a mortality of 60%-71.62%. However, the botanical biopesticides matrine 0.3% AS, azadirachtin 0.3% EC and rotenone 6% ME caused a much lower mortalities(<42%). In conclusion, the spinosad, abamectin, Bacillus thuringiensis and Beauveria bassiana are recommended for the control of field S. frugiperda populations.
引文
[1] LUGINBILL P.The fall army worm [R].USDA Technology Bulletin,1928,34:2-7.
[2] 姜玉英,刘杰,朱晓明.草地贪夜蛾侵入我国的发生动态和未来趋势分析[J].中国植保导刊,2019,39(2):33-35.
[3] 吴秋琳,姜玉英,吴孔明.草地贪夜蛾缅甸虫源迁入中国的路径分析[J].植物保护,2019,45(2):1-6.
[4] 全国农业技术推广服务中心.植物病虫情报[EB/OL].2019.https://www.natesc.org.cn/Html/2019_05_06/28092_151760_2019_05_06_458341.html.
[5] Food and Agriculture Organization of the United Nations.Integrated management of the fall armyworm on maize.A guide for farmer field schools in Africa [R/OL].2018.http://www.fao.org/3/i8741en/I8741EN.pdf.
[6] United States Agency for International Development,The International Maize and Wheat Improvement Center,The CGIAR Research Program on Maize.Fall armyworm in Africa:a guide for integrated pest management[R/OL].2018.https://reliefweb.int/sites/reliefweb.int/files/resources/FallArmyworm_IPM_Guide_forAfrica.pdf.
[7] BURTET L M,BERNARDI O,MELO A A,et al.Managing fall armyworm,Spodoptera frugiperda (Lepidoptera:Noctuidae),with Bt maize and insecticides in south Brazil [J].Pest Management Science,2017,73(12):2569-2577.
[8] LABATTE J M.Within-plant distribution of fall armyworm (Lepidoptera:Noctuidae) larvae on corn during whorl-stage infestation [J].Florida Entomologist,1993,76(3):437-447.
[9] ARAHAMS P,BEALE T,COCK M,et al.Fall armyworm status:Impacts and control options in Africa:Preliminary evidence note (April 2017) [R].CABI,UK,2017.
[10] YOUNG J R,MCMILLIAN W W.Differential feeding by two strains of fall armyworm larvae on carbaryl treated surfaces[J].Journal of Economic Entomology,1979,72(2):202-203.
[11] YU S J.Detection and biochemical characterization of insecticide resistance in fall armyworm (Lepidoptera:Noctuidae)[J].Journal of Economic Entomology,1992,85(3):675-682.
[12] YU S J,NGUYEN S N,ABO-ELGHAR G E.Biochemical characteristics of insecticide resistance in the fall armyworm,Spodoptera frugiperda (J.E.Smith)[J].Pesticide Biochemistry and Physiology,2003,77(1):1-11.
[13] BERTA C D,VIRLA E,COLOMO M V,et al.Efecto en el parasitoide Campoletis grioti de un insecticida usado para el control de Spodoptera frugiperda y aportes a la bionomía del parasitoide [J].Manejo Integrado de Plagas (CATIE),2000(57):65-70.
[14] DIEZ-RODRíGUEZ G I,OMOTO C.Inheritance of lambda-cyhalothrin resistance in Spodoptera frugiperda (J.E.Smith) (Lepidoptera:Noctuidae)[J].Neotropical Entomology,2001,30(2):311-316.
[15] CARVALHO R A,OMOTO C,FIELD L M,et al.Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda[J/OL].PLoS ONE,2013,8(4):e62268.
[16] NASCIMENTO A R B,FARIAS J R,BERNARDI D,et al.Genetic basis of Spodoptera frugiperda (Lepidoptera:Noctuidae) resistance to the chitin synthesis inhibitor lufenuron [J].Pest Management Science,2016,72(4):810-815.
[17] 吴孔明.中国农作物病虫害防控科技的发展方向[J].农学学报,2018,8(1):35-38.
[18] 徐汉虹,安玉兴.生物农药的发展动态与趋势展望[J].农药科学与管理,2001(1):32-34.
[19] 邵仁志,刘小安,孙兰,等.中国植物源农药的研究进展[J].湖北农业科学,2017,56(8):1401-1405.
[20] 袁梅,徐汉虹.未来治理害物抗性的工具[J].世界农药,2013,35(1):8-11.
[21] SILVA M C,SIQUEIRA H,SILVA L M,et al.Cry proteins from Bacillus thuringiensis active against diamondback moth and fall armyworm [J].Neotropical Entomology,2015,44(4):392-401.
[22] ISMAN M B,GRIENEISEN M L.Botanical insecticide research:many publications,limited useful data [J].Trends in Plant Science,2014,19(3):140-145.
[23] ZANARDI O Z,RIBEIRO L D P,ANSANTE T F,et al.Bioactivity of a matrine-based biopesticide against four pest species of agricultural importance [J].Crop Protection,2015,67:160-167.
[24] TAVARES W S,COSTA M A,CRUZ I,et al.Selective effects of natural and synthetic insecticides on mortality of Spodoptera frugiperda (Lepidoptera:Noctuidae) and its predator Eriopis connexa (Coleoptera:Coccinellidae)[J].Journal of Environmental Science and Health Part B,2010,45(6):557-561.
[25] 中华人民共和国农业部.农药室内生物测定试验准则.杀虫剂.第5部分:杀卵活性试验浸渍法:NY/T 1154.5-2006[S].北京:中国农业出版社,2006.
[26] 中华人民共和国农业部.农药室内生物测定试验准则.杀虫剂.第14部分:浸叶法:NY/T 1154.14-2008[S].北京:中国农业出版社,2008.
[27] 中华人民共和国农业部.农药室内生物测定试验准则.杀虫剂.第6部分:杀虫活性试验.浸虫法:NY/T 1154.6-2006[S].北京:中国农业出版社,2006.
[28] OKUMA D M,BERNARDI D,HORIKOSHI R J,et al.Inheritance and fitness costs of Spodoptera frugiperda (Lepidoptera:Noctuidae) resistance to spinosad in Brazil [J].Pest Management Science,2018,74(6):1441-1448.
[29] CARNEIRO A A,GOMES E A,GUIMAR?ES C T.Molecular characterization and pathogenicity of isolates of Beauveria spp.to fall armyworm [J].Pesquisa Agropecuária Brasileira,2008,43(4):513-520.
[30] THOMAZONI D,FORMENTINI M A,ALVES L F A.Patogenicity of entomopathogenic fungi to Spodoptera frugiperda (Smith) (Lepidoptera:Noctuidae)[J].Arquivos do Instituto Biológico,2014,81(2):126-133.
[31] GARCíA G C,GONZáLEZ M M B,BAUTISTA M N.Pathogenicity of isolates of entomopathogenic fungi against Spodoptera frugiperda (Lepidoptera:Noctuidae) and Epilachna varivestis (Coleoptera:Coccinellidae)[J].Revista Colombiana de Entomología,2011,37(2):217-222.
[32] MONNERAT R G,BATISTA A C,DE MEDEIROS P T,et al.Screening of Brazilian Bacillus thuringiensis isolates active against Spodoptera frugiperda,Plutella xylostella and Anticarsia gemmatalis [J].Biological Control,2007,41(3):291-295.
[33] LI Z H,HUANG R L,LI W S,et al.Addition of cinnamon oil improves toxicity of rotenone to Spodoptera litura (Lepidoptera:Noctuidae) larvae[J].Florida Entomologist,2017,100(3):515-521.
[34] MOSCARDI F.Assessment of the application of baculoviruses for control of lepidoptera [J].Annual Review of Entomology,1999,44(1):257-289.
[35] MENDEZ W V J,IBARRA J E,CISNEROS J,et al.Spinosad and nucleopolyhedrovirus mixtures for control of Spodoptera frugiperda (Lepidoptera:Noctuidae) in maize[J].Biological Control,2002,25(2):195-206.
[36] RAMEZ-GUERRA P,TAMAYO-MEJíA F,GOMEZ-FLORES R,et al.Increased efficacy and extended shelf life of spinosad formulated in phagostimulant granules against Spodoptera frugiperda[J].Pest Management Science,2017,74(1):100-110.
[37] JACHSON D M,SHAPIRO M,SHEPARD B M.Effects of spinosad and neem on the efficacy of a nucleopolyhedrovirus on pickleworm larvae [J].Journal of Agricultural and Urban Entomology,2014,30(30):28-37.
[38] RIVERO-BORJA M R,GUZMáN-FRANCO A W,RODRíGUEZ-LEYVA E,et al.Interaction of Beauveria bassiana and Metarhizium anisopliae with chlorpyrifos ethyl and spinosad in Spodoptera frugiperda larvae [J].Pest Management Science,2018,74(9):2047-2052.
[2] 姜玉英,刘杰,朱晓明.草地贪夜蛾侵入我国的发生动态和未来趋势分析[J].中国植保导刊,2019,39(2):33-35.
[3] 吴秋琳,姜玉英,吴孔明.草地贪夜蛾缅甸虫源迁入中国的路径分析[J].植物保护,2019,45(2):1-6.
[4] 全国农业技术推广服务中心.植物病虫情报[EB/OL].2019.https://www.natesc.org.cn/Html/2019_05_06/28092_151760_2019_05_06_458341.html.
[5] Food and Agriculture Organization of the United Nations.Integrated management of the fall armyworm on maize.A guide for farmer field schools in Africa [R/OL].2018.http://www.fao.org/3/i8741en/I8741EN.pdf.
[6] United States Agency for International Development,The International Maize and Wheat Improvement Center,The CGIAR Research Program on Maize.Fall armyworm in Africa:a guide for integrated pest management[R/OL].2018.https://reliefweb.int/sites/reliefweb.int/files/resources/FallArmyworm_IPM_Guide_forAfrica.pdf.
[7] BURTET L M,BERNARDI O,MELO A A,et al.Managing fall armyworm,Spodoptera frugiperda (Lepidoptera:Noctuidae),with Bt maize and insecticides in south Brazil [J].Pest Management Science,2017,73(12):2569-2577.
[8] LABATTE J M.Within-plant distribution of fall armyworm (Lepidoptera:Noctuidae) larvae on corn during whorl-stage infestation [J].Florida Entomologist,1993,76(3):437-447.
[9] ARAHAMS P,BEALE T,COCK M,et al.Fall armyworm status:Impacts and control options in Africa:Preliminary evidence note (April 2017) [R].CABI,UK,2017.
[10] YOUNG J R,MCMILLIAN W W.Differential feeding by two strains of fall armyworm larvae on carbaryl treated surfaces[J].Journal of Economic Entomology,1979,72(2):202-203.
[11] YU S J.Detection and biochemical characterization of insecticide resistance in fall armyworm (Lepidoptera:Noctuidae)[J].Journal of Economic Entomology,1992,85(3):675-682.
[12] YU S J,NGUYEN S N,ABO-ELGHAR G E.Biochemical characteristics of insecticide resistance in the fall armyworm,Spodoptera frugiperda (J.E.Smith)[J].Pesticide Biochemistry and Physiology,2003,77(1):1-11.
[13] BERTA C D,VIRLA E,COLOMO M V,et al.Efecto en el parasitoide Campoletis grioti de un insecticida usado para el control de Spodoptera frugiperda y aportes a la bionomía del parasitoide [J].Manejo Integrado de Plagas (CATIE),2000(57):65-70.
[14] DIEZ-RODRíGUEZ G I,OMOTO C.Inheritance of lambda-cyhalothrin resistance in Spodoptera frugiperda (J.E.Smith) (Lepidoptera:Noctuidae)[J].Neotropical Entomology,2001,30(2):311-316.
[15] CARVALHO R A,OMOTO C,FIELD L M,et al.Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda[J/OL].PLoS ONE,2013,8(4):e62268.
[16] NASCIMENTO A R B,FARIAS J R,BERNARDI D,et al.Genetic basis of Spodoptera frugiperda (Lepidoptera:Noctuidae) resistance to the chitin synthesis inhibitor lufenuron [J].Pest Management Science,2016,72(4):810-815.
[17] 吴孔明.中国农作物病虫害防控科技的发展方向[J].农学学报,2018,8(1):35-38.
[18] 徐汉虹,安玉兴.生物农药的发展动态与趋势展望[J].农药科学与管理,2001(1):32-34.
[19] 邵仁志,刘小安,孙兰,等.中国植物源农药的研究进展[J].湖北农业科学,2017,56(8):1401-1405.
[20] 袁梅,徐汉虹.未来治理害物抗性的工具[J].世界农药,2013,35(1):8-11.
[21] SILVA M C,SIQUEIRA H,SILVA L M,et al.Cry proteins from Bacillus thuringiensis active against diamondback moth and fall armyworm [J].Neotropical Entomology,2015,44(4):392-401.
[22] ISMAN M B,GRIENEISEN M L.Botanical insecticide research:many publications,limited useful data [J].Trends in Plant Science,2014,19(3):140-145.
[23] ZANARDI O Z,RIBEIRO L D P,ANSANTE T F,et al.Bioactivity of a matrine-based biopesticide against four pest species of agricultural importance [J].Crop Protection,2015,67:160-167.
[24] TAVARES W S,COSTA M A,CRUZ I,et al.Selective effects of natural and synthetic insecticides on mortality of Spodoptera frugiperda (Lepidoptera:Noctuidae) and its predator Eriopis connexa (Coleoptera:Coccinellidae)[J].Journal of Environmental Science and Health Part B,2010,45(6):557-561.
[25] 中华人民共和国农业部.农药室内生物测定试验准则.杀虫剂.第5部分:杀卵活性试验浸渍法:NY/T 1154.5-2006[S].北京:中国农业出版社,2006.
[26] 中华人民共和国农业部.农药室内生物测定试验准则.杀虫剂.第14部分:浸叶法:NY/T 1154.14-2008[S].北京:中国农业出版社,2008.
[27] 中华人民共和国农业部.农药室内生物测定试验准则.杀虫剂.第6部分:杀虫活性试验.浸虫法:NY/T 1154.6-2006[S].北京:中国农业出版社,2006.
[28] OKUMA D M,BERNARDI D,HORIKOSHI R J,et al.Inheritance and fitness costs of Spodoptera frugiperda (Lepidoptera:Noctuidae) resistance to spinosad in Brazil [J].Pest Management Science,2018,74(6):1441-1448.
[29] CARNEIRO A A,GOMES E A,GUIMAR?ES C T.Molecular characterization and pathogenicity of isolates of Beauveria spp.to fall armyworm [J].Pesquisa Agropecuária Brasileira,2008,43(4):513-520.
[30] THOMAZONI D,FORMENTINI M A,ALVES L F A.Patogenicity of entomopathogenic fungi to Spodoptera frugiperda (Smith) (Lepidoptera:Noctuidae)[J].Arquivos do Instituto Biológico,2014,81(2):126-133.
[31] GARCíA G C,GONZáLEZ M M B,BAUTISTA M N.Pathogenicity of isolates of entomopathogenic fungi against Spodoptera frugiperda (Lepidoptera:Noctuidae) and Epilachna varivestis (Coleoptera:Coccinellidae)[J].Revista Colombiana de Entomología,2011,37(2):217-222.
[32] MONNERAT R G,BATISTA A C,DE MEDEIROS P T,et al.Screening of Brazilian Bacillus thuringiensis isolates active against Spodoptera frugiperda,Plutella xylostella and Anticarsia gemmatalis [J].Biological Control,2007,41(3):291-295.
[33] LI Z H,HUANG R L,LI W S,et al.Addition of cinnamon oil improves toxicity of rotenone to Spodoptera litura (Lepidoptera:Noctuidae) larvae[J].Florida Entomologist,2017,100(3):515-521.
[34] MOSCARDI F.Assessment of the application of baculoviruses for control of lepidoptera [J].Annual Review of Entomology,1999,44(1):257-289.
[35] MENDEZ W V J,IBARRA J E,CISNEROS J,et al.Spinosad and nucleopolyhedrovirus mixtures for control of Spodoptera frugiperda (Lepidoptera:Noctuidae) in maize[J].Biological Control,2002,25(2):195-206.
[36] RAMEZ-GUERRA P,TAMAYO-MEJíA F,GOMEZ-FLORES R,et al.Increased efficacy and extended shelf life of spinosad formulated in phagostimulant granules against Spodoptera frugiperda[J].Pest Management Science,2017,74(1):100-110.
[37] JACHSON D M,SHAPIRO M,SHEPARD B M.Effects of spinosad and neem on the efficacy of a nucleopolyhedrovirus on pickleworm larvae [J].Journal of Agricultural and Urban Entomology,2014,30(30):28-37.
[38] RIVERO-BORJA M R,GUZMáN-FRANCO A W,RODRíGUEZ-LEYVA E,et al.Interaction of Beauveria bassiana and Metarhizium anisopliae with chlorpyrifos ethyl and spinosad in Spodoptera frugiperda larvae [J].Pest Management Science,2018,74(9):2047-2052.