烟草PP2C家族基因降雨量响应表达模式分析
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摘要
PP2C是一类单体丝氨酸/苏氨酸蛋白磷酸酶,广泛参与生物体内多个信号转导。PP2C基因家族的鉴定和功能研究已在多种作物上报道。为探明不同降雨量条件下烟草PP2C基因家族表达特征,通过塑料大棚内模拟人工定量喷灌试验和不同降雨量的田间试验,利用RNA-Seq技术对移栽后40和60d烟叶中PP2C家族基因进行了表达分析。结果显示,18个烟草PP2C家族基因的表达明显受降雨量影响,可分成9个亚族。其中6个PP2C家族成员的表达随着降雨量增大而降低,4个PP2C家族成员的表达随着降雨量增大而升高。本研究初步探明了云烟87中受降雨量影响的PP2C基因家族种类及其表达特点,为抗旱育种和抗旱栽培提供参考。
PP2C is a class of monomeric serine/threonine protein phosphatases that are widely involved in multiple signal transduction pathways in organisms. Identification and functional studies of the PP2C gene family have been reported in a variety of crops. In order to ascertain the expression characteristics of tobacco PP2C gene family members under different rainfall conditions,the expression of PP2C family genes in tobacco leaves at 40 and 60 days after transplanting was analyzed by RNA-Seq technique through simulated artificial quantitative irrigation in plastic greenhouse and field experiments with different rainfall. The results showed that the expression of 18 tobacco PP2C family genes was significantly affected by rainfall and could be divided into 9 subfamilies. The expression of six PP2C family members decreased with increasing rainfall, and the expression of four PP2C family members increased with increasing rainfall. In this study we have preliminarily identified the types and expression characteristics of the PP2C gene family affected by rainfall in Yunyan 87, providing reference for drought-resistant breeding and drought-resistant cultivation.
PP2C is a class of monomeric serine/threonine protein phosphatases that are widely involved in multiple signal transduction pathways in organisms. Identification and functional studies of the PP2C gene family have been reported in a variety of crops. In order to ascertain the expression characteristics of tobacco PP2C gene family members under different rainfall conditions,the expression of PP2C family genes in tobacco leaves at 40 and 60 days after transplanting was analyzed by RNA-Seq technique through simulated artificial quantitative irrigation in plastic greenhouse and field experiments with different rainfall. The results showed that the expression of 18 tobacco PP2C family genes was significantly affected by rainfall and could be divided into 9 subfamilies. The expression of six PP2C family members decreased with increasing rainfall, and the expression of four PP2C family members increased with increasing rainfall. In this study we have preliminarily identified the types and expression characteristics of the PP2C gene family affected by rainfall in Yunyan 87, providing reference for drought-resistant breeding and drought-resistant cultivation.
引文
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[17]胡伟,颜彦,何艳臻.小麦TaPP2C59基因的克隆及表达分析[J].麦类作物学报,2014,34(10):1334-1340.HU W,YAN Y,HE Y Z.Cloning and expression analysis of TaPP2C59 gene in wheat[J].Journal of Triticeae Crops,2014,34(10):1334-1340.
[18]ZHANG J H,LI X S,HE Z M,et al.Molecular character of a phosphatase 2C(PP2C)gene relation to stress tolerance in Arabidopsis thaliana[J].Molecular Biology Reports,2013,40(3):2633-2644.
[19]CUI M H,YOO K S,HYOUNG S,et al.An Arabidopsis R2R2-MYB transcription factor,AtMYB20,negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance[J].FEBS Letters,2013,587(12):1773-1778.
[20]LEUNG J,MERLOT S,GIRAUDAT J.The Arabidopsis ABSCISIC ACID-INSENSITIVE2(ABI2)and ABI1genes encode homologous protein phosphatases 2Cinvolved in abscisic acid signal transduction[J].The Plant Cell,1997,9(5):759-771.
[21]GOSTI F,BEAUDOIN N,SERIZET C,et al.ABI1protein phosphatase 2C is a negative regulator of abscisic acid signaling[J].The Plant Cell,1999,11(10):1897-1909.
[22]SCHWEIGHOFER A,KAZANAVICIUTE V,SCHEIKLE,et al.The PP2C-type phosphatase AP2C1,which negatively regulates MPK4 and MPK6,modulates innate immunity,jasmonic acid,and ethylene levels in Arabidopsis[J].The Plant Cell,2007,19(7):2213-2224.
[23]MESKIENE I,BAUDOUIN E,SCHWEIGHOFER A,et al.Stress-induced protein phosphatase 2C is a negative regulator of a mitogen-activated protein kinase[J].Journal of Biological Chemistry,2003,278(21):18945-18952.
[24]郭鹏,张士刚,邢鑫,等.欧美杨PdPP2C基因的克隆与功能分析[J].北京林业大学学报,2015,37(2):100-106.GUO P,ZHANG S G,XING X,et al.Cloning and function analysis of PdPP2C from Popululus deltoises[J].Journal of Beijing Forestry University,2015,37(2):100-106.
[25]LIU X,ZHU Y,ZHAI H,et al.AtPP2CG1,a protein phosphatase 2C,positively regulates salt tolerance of Arabidopsis in abscisic acid-dependent manner[J].Biochemical&Biophysical Research Communications,2012,422(4):710.
[26]徐志超,浦香东,宋经元.基于丹参基因组的蛋白磷酸酶2C家族的系统分析[J].中国现代中药,2018,20(6):652-657.XU Z C,PU X D,SONG J Y.Genome-wide characterization of PP2C gene family in Salvia miltiorrhiza[J].Mod Chin Med,2018,20(6):652-657.
[27]赵娜.中间锦鸡儿CiPP2C37-like基因的克隆和功能分析[D].呼和浩特:内蒙古农业大学,2016.ZHAO N.Cloning and function analysis of CiPP2C37-like gene from Caragana intermedia[D].Hohhot:Inner Mongolia Agriculture University,2016.
[2]LUAN S.Protein phosphatases in plants[J].Annual Review of Plant Biology,2003,54(54):63-92.
[3]STERN A,PRIVMAN E,RASIS M,et al.Evolution of the metazoan protein phosphatase 2C superfamily[J].Journal of Molecular Evolution,2007,64:61-70.
[4]COHEN P.The structure and regijlation of protein phosphatases[J].Annu Rev Biochem,1989,58:453-508.
[5]CAO J M,JIANG M,LI P,et al.Genome-wide identification and evolutionary analyses of the PP2C gene family with their expression profiling in response to multiple stresses in Brachypodium distachyon[J].BMCGenomics,2016,17(1):175.
[6]SHEEN J.Mutational analysis of protein phosphatase 2Cinvolved in abscisic acid signal transduction in higher plants[J].Proc Natl Acad Sci USA,1998,95:975-980.
[7]NISHIMURA N,OKAMOTO M,NARUSAKA M,et al.ABA hypersensitive germination2-1 causes the activation of both abscisic acid and salicylic acid responses in Arabidopsis[J].Plant Cell Physiol,2009,50:2112-2122.
[8]HONG S W,JON J H,KWAK J M.Identification of a receptor-like protein kinase gene rapidly induced by abacisic acid,dehydration,high salt and cold treatments in Arabidopsis thaliana[J].Plant Physiology,1997,113(4):1203-1212.
[9]XUE T,WANG D,ZHANG S,et al.Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis[J].BMC Genomics,2008,9(1):550.
[10]张继红,陶能国.植物PP2C蛋白磷酸酶ABA信号转导及逆境胁迫调控机制研究进展[J].广西植物,2015,35(6):935-941.ZHANG J H,TAO N G.Research progress on regulation mechanism of plant PP2C protein phosphatase ABAsignal transduction and abiotic stress[J].The Plants in Guangxi,2015,35(6):935-941.
[11]张柳,何晓健,林春,等.降雨量对“云烟87”叶片基因表达影响的分析[J].分子植物育种,2018,16(16):5483-5504.ZHANG L,HE X J,LIN C,et al.Analysis of the influence of rainfall on gene expression in leaves of Yunyan 87[J].Molecular Plant Breeding,2018,16(16):5483-5504.
[12]李国芸,李志伟,甄焕菊,等.水分胁迫条件下烟草生理生化响应研究进展[J].中国农学通报,2007,23(9):298-301.LI G Y,LI Z W,ZHEN H J,et al.Research advance on physiological and biochemical reponses in tobacco under water stress[J].Chinese Agricultural Bulletin,2007,23(9):298-301.
[13]张雪薇,刘仑,鲁黎明,等.烟草磷酸酶基因NtPP2C16的克隆、表达载体构建及表达分析[J].华北农学报,2017,32(5):78-85.ZHANG X W,LIU L,LU L M,et al.Cloning construction of expression vector and expression analysis of NtPP2C16 in Nicotiana tabacum[J].Acta Agriculturae Boreali-Sinica,2017,32(5):78-85.
[14]COLE T,ADAM R,LOYAL G,et al.Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks[J].Nature Protocols,2012,7:562-578.
[15]XIANG L X,HE D,DONG W R,et al.Deep sequencing-based transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus reveals insight into the immune-relevant genes in marine fish[J].BMCgenomics,2010,11(1):472.
[16]LIU L,HU X,SONG J,et al.Over-expression of a Zea mays L.protein phosphatase 2C gene(ZmPP2C)in Arabidopsis thaliana decreases tolerance to salt and drought[J].Journal of Plant Physiology,2009,166(5):531-542.
[17]胡伟,颜彦,何艳臻.小麦TaPP2C59基因的克隆及表达分析[J].麦类作物学报,2014,34(10):1334-1340.HU W,YAN Y,HE Y Z.Cloning and expression analysis of TaPP2C59 gene in wheat[J].Journal of Triticeae Crops,2014,34(10):1334-1340.
[18]ZHANG J H,LI X S,HE Z M,et al.Molecular character of a phosphatase 2C(PP2C)gene relation to stress tolerance in Arabidopsis thaliana[J].Molecular Biology Reports,2013,40(3):2633-2644.
[19]CUI M H,YOO K S,HYOUNG S,et al.An Arabidopsis R2R2-MYB transcription factor,AtMYB20,negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance[J].FEBS Letters,2013,587(12):1773-1778.
[20]LEUNG J,MERLOT S,GIRAUDAT J.The Arabidopsis ABSCISIC ACID-INSENSITIVE2(ABI2)and ABI1genes encode homologous protein phosphatases 2Cinvolved in abscisic acid signal transduction[J].The Plant Cell,1997,9(5):759-771.
[21]GOSTI F,BEAUDOIN N,SERIZET C,et al.ABI1protein phosphatase 2C is a negative regulator of abscisic acid signaling[J].The Plant Cell,1999,11(10):1897-1909.
[22]SCHWEIGHOFER A,KAZANAVICIUTE V,SCHEIKLE,et al.The PP2C-type phosphatase AP2C1,which negatively regulates MPK4 and MPK6,modulates innate immunity,jasmonic acid,and ethylene levels in Arabidopsis[J].The Plant Cell,2007,19(7):2213-2224.
[23]MESKIENE I,BAUDOUIN E,SCHWEIGHOFER A,et al.Stress-induced protein phosphatase 2C is a negative regulator of a mitogen-activated protein kinase[J].Journal of Biological Chemistry,2003,278(21):18945-18952.
[24]郭鹏,张士刚,邢鑫,等.欧美杨PdPP2C基因的克隆与功能分析[J].北京林业大学学报,2015,37(2):100-106.GUO P,ZHANG S G,XING X,et al.Cloning and function analysis of PdPP2C from Popululus deltoises[J].Journal of Beijing Forestry University,2015,37(2):100-106.
[25]LIU X,ZHU Y,ZHAI H,et al.AtPP2CG1,a protein phosphatase 2C,positively regulates salt tolerance of Arabidopsis in abscisic acid-dependent manner[J].Biochemical&Biophysical Research Communications,2012,422(4):710.
[26]徐志超,浦香东,宋经元.基于丹参基因组的蛋白磷酸酶2C家族的系统分析[J].中国现代中药,2018,20(6):652-657.XU Z C,PU X D,SONG J Y.Genome-wide characterization of PP2C gene family in Salvia miltiorrhiza[J].Mod Chin Med,2018,20(6):652-657.
[27]赵娜.中间锦鸡儿CiPP2C37-like基因的克隆和功能分析[D].呼和浩特:内蒙古农业大学,2016.ZHAO N.Cloning and function analysis of CiPP2C37-like gene from Caragana intermedia[D].Hohhot:Inner Mongolia Agriculture University,2016.