HPLC法同时测定维血宁中3种化学成分的含量
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摘要
目的:建立同时测定维血宁中虎杖苷、大黄素-8-O-β-D葡萄糖苷及大黄素含量的方法。方法:应用HPLC法测定,色谱柱:Kromasil C18Column(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;流速:1.0 mL·min-1;柱温:30℃;检测波长:288 nm。结果:虎杖苷、大黄素-8-O-β-D葡萄糖苷和大黄素的线性范围分别为0.09-0.72μg(r=0.9984)、0.4825-3.86μg(r=1.0000)、0.2675-2.14μg(r=1.0000);平均加样回收率分别为98.9%(RSD%=2.8%)、99.6%(RSD%=0.16%)、98.1%(RSD%=0.44%)。结论:该方法同时测定维血宁中的虎杖苷、大黄素-8-O-β-D葡萄糖苷和大黄素的含量,简便易行、准确、重复性好,可为维血宁的质量控制研究提供一种可靠的参考方法。
Objective: To establish a high performance liquid chromatographic method for simultaneous determination of the contents of Polydatin,Emodin-8-O-β-D-glycosidase and Emodin in Weixuening.Methods: HPLC was applied with the chromatographic condition as follows;the chromatographic column was Kromasil C18 Column(4.6 mm×250 mm,5 μm);acetonitrile0.1% H3 PO4 as the mobile phrase with gradient elution;flow rate was 1.0 mL·min-1;the detection wave length was 288 nm;temperature was 30 ℃.Results: Linearity relationships were 0.09-0.72 μg(r=0.9984),0.4825-3.86 μg(r=1.0000) and 0.26752.14 μg(r=1.0000),respectively of Polydatin,Emodin-8-O-β-D-glycosidase and Emodin,as well as the average recoveries of 98.9%(RSD%=2.8%),99.6%(RSD%=0.16%) and 98.1%(RSD%=0.44%).Conclusion: The results showed that the method is simple,accurate and repeatable and that it is suitable for simultaneous determining the contents of Polydatin,Emodin-8-O-βD-glycosidase and Emodin in Weixuening.
Objective: To establish a high performance liquid chromatographic method for simultaneous determination of the contents of Polydatin,Emodin-8-O-β-D-glycosidase and Emodin in Weixuening.Methods: HPLC was applied with the chromatographic condition as follows;the chromatographic column was Kromasil C18 Column(4.6 mm×250 mm,5 μm);acetonitrile0.1% H3 PO4 as the mobile phrase with gradient elution;flow rate was 1.0 mL·min-1;the detection wave length was 288 nm;temperature was 30 ℃.Results: Linearity relationships were 0.09-0.72 μg(r=0.9984),0.4825-3.86 μg(r=1.0000) and 0.26752.14 μg(r=1.0000),respectively of Polydatin,Emodin-8-O-β-D-glycosidase and Emodin,as well as the average recoveries of 98.9%(RSD%=2.8%),99.6%(RSD%=0.16%) and 98.1%(RSD%=0.44%).Conclusion: The results showed that the method is simple,accurate and repeatable and that it is suitable for simultaneous determining the contents of Polydatin,Emodin-8-O-βD-glycosidase and Emodin in Weixuening.
引文
[1]杨洁文,刘云霞,王翌庆,等.维血宁颗粒联合巨和粒治疗化疗后血小板减少症30例疗效观察[J].浙江中医杂志,2013,48(1):74
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[4]袁萍,高俊飞,袁晓.HPLC-DAD测定虎杖中八种成分含量及指纹图谱研究[J].中国现代应用药学,2013,30(1):20-24
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[2]曹林林,田正良.维血宁合剂治疗抗甲状腺药物导致白细胞减少症临床观察[J].时珍国医国药,2011,22(8):1971
[3]顾峥嵘,承晨.HPLC法测定维血宁颗粒中大黄素的含量[J].江苏药学与临床研究,2005,13(4):27-29
[4]袁萍,高俊飞,袁晓.HPLC-DAD测定虎杖中八种成分含量及指纹图谱研究[J].中国现代应用药学,2013,30(1):20-24
[5]张明昶,彭小冰,苗旭辉.中药虎杖特征指纹图谱研究[J].中国实验方剂学杂志,2011,17(1):53-55
[6]孔晓华.中药虎杖的研究进展[J].中医药导报,2009,15(5):107-110