一株碱蓬内生真菌的鉴定及促生活性产物的初步研究
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摘要
对辽宁盘锦红海滩的盐生碱蓬内生真菌JP4-1进行鉴定并研究其促生活性产物成分。根据形态学特征及18S r DNA序列分析对菌种进行鉴定,运用过滤离心、超声破碎、萃取、旋蒸等技术提取活性成分,并采用水培法研究该菌株发酵液对水稻幼苗的促生长活性。结果显示,经鉴定,JP4-1菌株为小丛壳属(Glomerella sp.),菌丝分枝,有隔,产孢;以正丁醇处理的JP4-1菌株胞外发酵液的小分子萃取物可使水稻幼苗的株高和叶片干重分别提高10.84%和14.67%,叶绿素含量提高44.22%,促生长效果明显。碱蓬内生真菌JP4-1的胞外发酵产物对水稻幼苗具有明显的促生长作用,其中以正丁醇萃取得到的小分子活性产物促生效果最为显著。
The purposes of this work are to identify the endophyte fungus JP4-1 of Suaeda salsa in Red Beach of Panjin,Liaoning Province,and to research the growth promotion activity of its metabolites. Endophytic fungus JP4-1 was identified using its morphological features and 18 S r DNA,and several technical means such as filtering centrifuge,ultrasonic crushing,extraction,and rotary evaporateion were used to extract its active components,and hydroponic experiments were taken to test the growth promotion activity of its fermentation broth to rice seedlings. The JP4-1 was identified as Glomerella sp. with hyphae branched,septate,and producing spores. The result of hydroponic experiment revealed that the micromolecular products extracted from fermented liquid with n-butanol promoted the plant height 10.84%,dry weight of leaves 14.67%,and chlorophyll content 44.22% of the rice seedlings,i.e.,the growth promotion effects were remarkable. In conclusion,the extracellular fermentation products by the endophyte fungus JP4-1 of S. salsa significantly promoted the growth of rice seedlings,and the micromolecular active products extracted by n-butanol had the most significant promotion effects.
The purposes of this work are to identify the endophyte fungus JP4-1 of Suaeda salsa in Red Beach of Panjin,Liaoning Province,and to research the growth promotion activity of its metabolites. Endophytic fungus JP4-1 was identified using its morphological features and 18 S r DNA,and several technical means such as filtering centrifuge,ultrasonic crushing,extraction,and rotary evaporateion were used to extract its active components,and hydroponic experiments were taken to test the growth promotion activity of its fermentation broth to rice seedlings. The JP4-1 was identified as Glomerella sp. with hyphae branched,septate,and producing spores. The result of hydroponic experiment revealed that the micromolecular products extracted from fermented liquid with n-butanol promoted the plant height 10.84%,dry weight of leaves 14.67%,and chlorophyll content 44.22% of the rice seedlings,i.e.,the growth promotion effects were remarkable. In conclusion,the extracellular fermentation products by the endophyte fungus JP4-1 of S. salsa significantly promoted the growth of rice seedlings,and the micromolecular active products extracted by n-butanol had the most significant promotion effects.
引文
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[3]Stierle A,Strobel G,Stierle D,et al.Taxol and taxane productionby Taxus brevifolia.An endophytic fungus of pacific yew[J].Science,1993,260(9):214-216.
[4]易晓华.植物内生真菌次生代谢产物研究进展[J].中国农学通报,2009,25(21):255-260.
[5]Soliman SS,Trobacher CP,Tsao R,et al.A fungal endophyte induces transcription of genes encoding a redundant fungicide pathway in its host plant[J].BMC Plant Biology,2013,26:13-93.
[6]赵可夫,范海,江行玉,等.盐生植物在盐渍土壤改良中的作用[J].应用与环境生物学报,2002,8(1):31-35.
[7]任立军.碱蓬[J].特种经济动植物,2004(4):26-27.
[8]魏磊.盐碱胁迫角果碱蓬幼苗的蛋白质组学研究[D].哈尔滨:东北林业大学,2012.
[9]唐永红,曹庸,卢成瑛,等.特殊生境微生物及其活性代谢产物研究进展[J].微生物学通报,2006,33(4):163-166.
[10]Khan AL,Waqas M,Khan AR,et al.Fungal endophyte Penicillium janthinellum LK5improves growth of ABA-deficient tomato under salinity[J].World J Microbiol Biotechnol,2013,29:2133-2144.
[11]赵颖,于飞,郭明敏,等.碱蓬内生真菌JP3的分离、鉴定及促生作用研究[J].沈阳师范大学学报:自然科学版,2015,33(1):116-120.
[12]禹乐乐.玉米内生真菌多样性及其宿主关系研究[D].郑州:郑州大学,2012.
[13]赵颖.碱蓬内生真菌生物多样性及促生活性JP3菌株的研究[D].沈阳:沈阳师范大学,2015.
[14]钮旭光,宋立超,韩梅,肖亦农.盐生植物翅碱蓬的内生真菌多样性分析[J].微生物学通报,2012,39(10):1388-1395.
[15]殷博,何鑫,曹亚彬.黑龙江省微生物肥料现状与问题[J].中国环境管理干部学院学报,2011,21(1):49-51.
[16]袁志林,戴传超,杨启银.微生物源的植物生长促进剂研究的新进展[J].土壤肥料,2004,6:41-46.
[17]王国基.根际促生专用菌肥研制及其对玉米促生作用的研究[D].兰州:甘肃农业大学,2014.
[18]王维.半夏内生菌的分离及其内生真菌对宿主生长影响的研究[D].杨凌:西北农林科技大学,2010.
[19]关丽,刘湘南,程承旗.土壤镉污染环境下水稻叶片叶绿素含量监测的高光谱遥感信息参数[J].光谱学与光谱分析,2009,10:2713-2716.
[20]袁志林.一株广谱内生真菌B3对水稻生长及生理影响研究[D].南京:南京师范大学,2005.