蒙药阿拉坦五味丸总黄酮的超声提取工艺研究
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摘要
目的:通过超声提取法研究蒙药阿拉坦五味丸中总黄酮的最佳提取工艺。方法:采用L9(34)正交实验,以总黄酮提取率为考察指标,考察乙醇浓度,料液比,超声时间和提取温度等因素对超声提取工艺的影响,确定其最佳提取工艺条件。结果:乙醇作溶剂对总黄酮的提取效率较高,且最佳提取工艺条件是:乙醇浓度70%,料液比1∶50,超声时间60min,超声温度60℃。结论:在该条件下,阿拉坦五味丸总黄酮的提取具有时间短、收率高、耗能小等优点。
Objective: To research the extraction of total flavonoids from Mongolian Medicine Altan flavorspill by ultrasonic extraction.Methods: Flavonoid content was used as the index,with L9(34) orthogonal experimental test,the effect of ethanol concentration,solid to liquid ratio,ultrasound time and extraction temperature on the extraction process was investigated for the optimal ultrasonic extraction conditions.Results: The ethanol extraction efficiency was higher,and the optimum extraction conditions was: 70% ethanol,solid to liquid ratio1:50,60 minutes of ultrasonic time,ultrasonic temperature of 60℃.Conclusion: The optimum extraction conditions were short time,high yield,energy consumption.
Objective: To research the extraction of total flavonoids from Mongolian Medicine Altan flavorspill by ultrasonic extraction.Methods: Flavonoid content was used as the index,with L9(34) orthogonal experimental test,the effect of ethanol concentration,solid to liquid ratio,ultrasound time and extraction temperature on the extraction process was investigated for the optimal ultrasonic extraction conditions.Results: The ethanol extraction efficiency was higher,and the optimum extraction conditions was: 70% ethanol,solid to liquid ratio1:50,60 minutes of ultrasonic time,ultrasonic temperature of 60℃.Conclusion: The optimum extraction conditions were short time,high yield,energy consumption.
引文
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[11]刘丽霞,包小妹,罗素琴,等.响应曲面法优化超声提取漏芦花中总黄酮的工艺[J].内蒙古医科大学学报,2016;38(5):393-397+400
[12]邬卫东,杨来秀,王玉华,等.蒙药小秦艽花总黄酮超声波提取工艺及其抗氧化研究[J].内蒙古师范大学学报(自然科学汉文版),2016;45(4):510-514
[13]陈玉兰,叶日贵,佟玉凤,等.蒙药肋柱花总黄酮提取工艺研究[J].时珍国医国药,2014;25(7):1544-1546
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[2]盛海燕,李雪芬.HPLC法测定阿拉坦五味丸中没食子酸的含量[J].中国药师,2009;12(11):1676-1677
[3]陶志国,祁进,刘颖.阿拉坦五味丸质量标准研究[J].中国中医药信息杂志,2013;20(9):57-60
[4]韩斯日古楞,王秀兰.蒙药阿拉坦五味散研究进展[J].内蒙古民族大学学报(自然科学版),2010;25(3):332-335
[5]VilkhuK,MawsonR,SimonsL,et al.Applications and opportunities for ultrasound assisted extraction in the food industry -A review[J].Innovative Food Science & Emerging Technologies,2008;9:161 -169
[6]云学英,高建萍.蒙药耧斗菜总黄酮提取条件的优化及含量测定[J].内蒙古农业大学学报(自然科学版),2015;36(1):1-5
[7]陈建平,王金辉,汤化琪,等.蒙药瞿麦中多糖超声提取工艺研究及含量测定[J].中南药学,2013;11(4):264-267
[8]许鑫,涂秀文,姜海燕,等.牻牛儿苗总黄酮提取工艺优化及含量测定研究[J].时珍国医国药,2012;38(8):1953-1955
[9]杨楠,韩国庆,高建萍.正交试验法优选狗尾草中总黄酮提取工艺研究[J].内蒙古医科大学学报,2017;39(3):207-210
[10]陈建平,王金辉,王艳杰,等.蒙药瞿麦中总黄酮超声提取工艺研究及含量测定[J].中华中医药杂志,2013;28(9):2580-2584
[11]刘丽霞,包小妹,罗素琴,等.响应曲面法优化超声提取漏芦花中总黄酮的工艺[J].内蒙古医科大学学报,2016;38(5):393-397+400
[12]邬卫东,杨来秀,王玉华,等.蒙药小秦艽花总黄酮超声波提取工艺及其抗氧化研究[J].内蒙古师范大学学报(自然科学汉文版),2016;45(4):510-514
[13]陈玉兰,叶日贵,佟玉凤,等.蒙药肋柱花总黄酮提取工艺研究[J].时珍国医国药,2014;25(7):1544-1546
[14]云学英,罗素琴,郭晓宇,等.蒙药云香十五味丸中黄酮类化合物含量的测定[J].内蒙古医科大学学报,2013;35(3):229-232
[15]李思阳,孔庆新,庄润泽,等.香椿子中总黄酮提取工艺的优化和比较[J].中成药,2017;39(10):2178-2182