越冬期邓恩桉嫁接种子园叶绿素与丙二醛动态变化
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摘要
以邓恩桉嫁接种子园抗寒能力不同且开花的5个家系为材料,测定其越冬期叶片中叶绿素与丙二醛(MDA)含量。结果表明:自然降温过程中,叶绿素含量随着温度的下降而降低,MDA含量随着温度的下降而增加,各家系MDA含量增加幅度不同;春季随着气温的回升,叶绿素含量略有上升,而MDA含量呈下降趋势。通过研究具开花能力的邓恩桉家系越冬期叶绿素与MDA含量动态变化,为选育抗寒能力强的邓恩桉F1代提供理论依据。
Five families of Eucalyptus dunnii that had been selected for flowering competence and a range of cold tolerance in a grafted seed orchard, had their leaves sampled at various times during the course of the winter months to examine their chlorophyll and malondialdehyde(MDA) contents. Chlorophyll contents were found to decrease as temperatures decreased as winter progressed, whilst MDA contents increased, but the increases in MDA differed among the five families. Then, with the onset of spring, chlorophyll contents increased while MDA contents decreased as the temperatures started to rise. This research on the dynamic changes in chlorophyll and MDA contents in the trees of the E. dunnii grafted seed orchard during winter will help provide a theoretical basis for the breeding of this species and production of high quality F1 seeds.
Five families of Eucalyptus dunnii that had been selected for flowering competence and a range of cold tolerance in a grafted seed orchard, had their leaves sampled at various times during the course of the winter months to examine their chlorophyll and malondialdehyde(MDA) contents. Chlorophyll contents were found to decrease as temperatures decreased as winter progressed, whilst MDA contents increased, but the increases in MDA differed among the five families. Then, with the onset of spring, chlorophyll contents increased while MDA contents decreased as the temperatures started to rise. This research on the dynamic changes in chlorophyll and MDA contents in the trees of the E. dunnii grafted seed orchard during winter will help provide a theoretical basis for the breeding of this species and production of high quality F1 seeds.
引文
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[25]刘彤,祝佳媛,李鹏,等.秋冬季自然降温过程中东北红豆杉幼苗的生理生化特性[J].北京林业大学学报,2013,35(2):51-56.
[26]王宁,姚方,袁美丽,等.自然降温对猴樟和芳樟叶绿素荧光参数的影响[J].河南农业大学学报,2013,47(4):414-419.
[27]刘奕清,李会合,陈泽雄.尾巨桉幼苗对低温胁迫的生理生化反应[J].福建林业科技,2007,34(4):24-26.
[28]刘建.两种桉树对低温胁迫的响应机制研究[D].南京:南京林业大学,2008.
[29]黄月华,徐建民,余雪标.低温胁迫对桉树代谢的影响[J].热带农业科学,2005,25(5):24-28.
[2]THOMAS D,HENSON M,JOE B,et al.Review of growth and wood quality of plantation-grown Eucalyptus dunnii Maiden[J].Australian Forestry,2009,72(1):3-11.
[3]王豁然.桉树生物学概论[M].北京:科学出版社,2010.
[4]祁述雄.中国桉树:第2版[M].北京:中国林业出版社,2002.
[5]罗建中.耐寒桉树良种-邓恩桉[J].桉树科技,2002(2):1-8.
[6]燕丽萍,夏阳,毛秀红等.邓恩桉的组织培养[J].林业科学,2011,47(5):157-161.
[7]谭柏韬,李茂娟,邓少华.邓恩桉再生系统的研究[J].中国农学通报,2013,29(31):32-35.
[8]郭东强,叶露,周维,等.2个种源邓恩桉木材纤维特性及变异[J].浙江农林大学学报,2014,31(4):502-507.
[9]任世奇,罗建中,彭彦,等.17年生邓恩桉两个种源木材密度与干缩性研究[J].亚热带植物科学,2010,39(2):5-9.
[10]叶露,NICHOLS J D,RAYMOND A D,等.邓恩桉生长特性与生长应变研究[J].安徽农业科学,2013,41(10):4409-4410,4418.
[11]卢翠香,陈建波,刘媛,等.邓恩桉生材含水率、年轮宽度及木材密度研究[J].桉树科技,2014,31(2):23-27.
[12]裘珍飞,曾炳山,刘英,等.应用电导法评价5个桉树抗寒性[J].广东林业科技,2011,27(3):27-31.
[13]郭祥泉,吴奇智,洪伟,等.应用电导率探讨邓恩桉不同优株的抗寒性[J].亚热带植物科学,2012,41(1):41-44.
[14]郭祥泉.用膜透性、SOD酶活性和膜脂脂肪酸成分探讨林木抗寒性[J].西南林业大学学报,2012,32(4):6-11.
[15]刘建,项东云,陈健波,等.低温胁迫对桉树光合和叶绿素荧光参数的影响[J].桉树科技,2009,26(1):1-6.
[16]靖长柏,张利阳,童再康,等.自然低温胁迫对3种桉树生理生化特性的影响[J].防护林科技,2010(6):18-19,35.
[17]卢新瑛.2011年初春持续低温对邓恩桉生长的影响[J].亚热带农业研究,2011,7(2):100-104.
[18]周修权,曹学良.桉树在郴州地区的抗寒性调查分析[J].湖南林业科技,2012,39(4):54-56.
[19]郭祥泉,郭祥堆.邓恩桉在闽北生长规律与适应性探讨[J].西北林学院学报,2010,25(2):50-55.
[20]郭祥泉,陈慈禄,洪伟,等.邓恩桉优株在闽北适应性种植决策探讨[J].西南林业大学学报,2012,32(3):26-30.
[21]李昌荣,郭东强,周维等.邓恩桉种子园开花生理生化特性比较[J].中国农学通报,2014,30(25):20-23.
[22]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[23]潘瑞炽.植物生理学:第5版[M].北京:高等教育出版社,2006.
[24]郭爱华,左宝峰,姚延梼,等.自然降温对雪松叶片中叶绿素及电导率的影响[J].山西农业大学学报(自然科学版),2005,25(4):393-395.
[25]刘彤,祝佳媛,李鹏,等.秋冬季自然降温过程中东北红豆杉幼苗的生理生化特性[J].北京林业大学学报,2013,35(2):51-56.
[26]王宁,姚方,袁美丽,等.自然降温对猴樟和芳樟叶绿素荧光参数的影响[J].河南农业大学学报,2013,47(4):414-419.
[27]刘奕清,李会合,陈泽雄.尾巨桉幼苗对低温胁迫的生理生化反应[J].福建林业科技,2007,34(4):24-26.
[28]刘建.两种桉树对低温胁迫的响应机制研究[D].南京:南京林业大学,2008.
[29]黄月华,徐建民,余雪标.低温胁迫对桉树代谢的影响[J].热带农业科学,2005,25(5):24-28.