巴什拜羊MC4R基因多态性及其与生长性状的关联分析
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摘要
试验旨在研究黑素皮质素受体-4(melanocortin-4 receptor,MC4R)基因在巴什拜羊中的多态性及其与生长性状的关系,探寻可用于巴什拜羊选育的分子遗传标记。试验以随机采取的300只巴什拜羊个体的血样作为试验材料,运用PCR-SSCP和DNA测序等技术对MC4R基因的部分外显子进行遗传多态性检测并与生长性状进行关联性分析。结果发现:巴什拜羊中MC4R基因有多态性,存在2种基因型:GG和GC,且处于Hardy-Weinberg平衡(P>0.05),多态信息含量为0.159,属于低度多态(PIC<0.25)。测序结果显示,与原基因碱基序列相比,MC4R基因碱基序列在893(G→C)处发生突变。与巴什拜羊生长性状进行关联分析可知,GC型个体的胸围和体重都显著高于GG型个体(P<0.05)。巴什拜羊MC4R基因在G893C处表现的多态有可能作为一种遗传标记。
In this study, in order to provide scientific basis for improving growth traits of Bashibai sheep by molecular marker assisted breeding methods, the relationship of the genetic polymorphisms of MC4 R gene and the growth traits was studied by molecular biology methods. The experiment were used in a random collection of 300 individual blood samples, using of PCR-SSCP analysis and DNA sequencing technology to analyzing polymorphism of MC4 R gene, and carrying out correlation analysis with growth traits.The results show that there is polymorphism in MC4 R gene the primer MC4 R gene existence two kinds of genotypes of GG and GC,and in the Hardy-Weinberg equilibrium state(P >0.05),polymorphism information content(PIC) was 0.159, belong to moderate polymorphism(PIC<0.25). Sequencing results show that, Compared with the original genetic base sequence, MC4 R genetic base sequence in the 893 G(C) point mutations.By correlation analysis it was found that the individual weight and Heart girth of GC genotype were munch higher than GG genotype(P<0.05).The polymorphism of G893 C on of MC4 R gene in Bashibai sheep may be used as a genetic marker.
In this study, in order to provide scientific basis for improving growth traits of Bashibai sheep by molecular marker assisted breeding methods, the relationship of the genetic polymorphisms of MC4 R gene and the growth traits was studied by molecular biology methods. The experiment were used in a random collection of 300 individual blood samples, using of PCR-SSCP analysis and DNA sequencing technology to analyzing polymorphism of MC4 R gene, and carrying out correlation analysis with growth traits.The results show that there is polymorphism in MC4 R gene the primer MC4 R gene existence two kinds of genotypes of GG and GC,and in the Hardy-Weinberg equilibrium state(P >0.05),polymorphism information content(PIC) was 0.159, belong to moderate polymorphism(PIC<0.25). Sequencing results show that, Compared with the original genetic base sequence, MC4 R genetic base sequence in the 893 G(C) point mutations.By correlation analysis it was found that the individual weight and Heart girth of GC genotype were munch higher than GG genotype(P<0.05).The polymorphism of G893 C on of MC4 R gene in Bashibai sheep may be used as a genetic marker.
引文
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[17]宋桃伟,蔡慧芬,罗卫星,等.两个贵州山羊品种GHSR和GHRL基因遗传变异及其与生长性状的关联性[J].中国农业科学,2015,48(l):140-153.
[2]刘娇娇.绵羊MC4R、MRF4、PROP1基因多态性及生物信息学分析[D].兰州:甘肃农业大学,2014.
[3]左北瑶,钱宏光.MC4R基因研究进展[J].中国草食动物,2011,31(5):45-50.
[4]国家畜禽遗传资源委员会组编.中国畜禽遗传资源志·羊志[M].北京:中国农业出版社,2011,122-123.
[5]买买提明·巴拉提,哈米提·哈凯莫夫,决肯·阿努瓦什.羔羊肉型巴什拜羊的研究[J].草食家畜,1999,(02):16-19.
[6]努尔拉提汗·木哈买提别克,赛力克·木勒达汗,决肯·阿尼瓦什,等.新疆巴什拜羊青色和白色毛绒品质比较分析[J].新疆畜牧业,2015,(11):25-27.
[7]周延清,杨清香,张改娜.生物遗传标记与应用[M].北京:化学工业出版社,2008,104-105.
[8]张英杰.羊生产学[M].北京:中国农业出版社,2010,252-253.
[9]张菊.绵羊CAST、MC4R和BTG1基因的克隆、组织表达和遗传多态性分析[D].北京:中国农业科学院,2009.
[10]张高振.MC4R基因多态性及其与湖羊早期生长性状的相关研究[D].南京:南京农业大学,2009,06.
[11]王春玲,茆达干,曹少先,等.6个绵羊群体MC4R基因CRS-PCR多态性及其与湖羊、东湖杂交羊生长性状的关联分析[J].南京:南京农业大学学报,2014,37(l):121-126.
[12]Komar A A.Silent SNPs:impact on gene function and phenotype[J].Pharmacogenomics,2007,8(8):1075-1080.
[13]任法鑫,杨钧国,李伟,等.国人SCN5A基因新的同义突变位点[J].中国循环杂志,2004,(05):52-54.
[14]陈南春,高辉,陈苏民,等.cⅠ857基因的体外定位同义突变[J].生物化学杂志,1994,(04):509-512.
[15]L.Corrado,S.Magri,A.Bagarotti,et al.A novel synonymous mutation in the MPZ gene causing an aberrant splicing pattern and Charcot-Marie-Tooth disease type 1b[J].Neuromuscular Disorders,2016.
[16]周明亮,张翔宇,綦松智,等.绵羊IGF-Ⅰ基因的表达及其SNPs与生长性状的相关分析研究[J].畜牧兽医学报,2013,44(09):1363-1370.
[17]宋桃伟,蔡慧芬,罗卫星,等.两个贵州山羊品种GHSR和GHRL基因遗传变异及其与生长性状的关联性[J].中国农业科学,2015,48(l):140-153.