三种不同来源白念珠菌感染对小鼠的致病性研究
|
摘要
目的:探讨3种不同来源白念珠菌对小鼠致病性的差异。方法:选取获得性免疫缺陷综合征(AIDS)、外阴阴道念珠菌病(VVC)患者来源和健康人来源白念珠菌尾静脉注射感染小鼠,同时设立生理盐水尾静脉注射小鼠作为对照组;分别于7~11 d、14~18 d及21~25 d 3个时期段,观察各组小鼠的死亡数及死亡率;无菌分离肾脏靶器官,观察各组小鼠的肾指数及肾脏负荷;收集从肾脏分离得到的白念珠菌,比较3种不同来源白念珠菌株菌丝形成能力。结果:3种不同来源菌株感染的小鼠28 d内死亡率、小鼠肾指数及其肾脏分离菌株菌丝形成能力差异有统计学意义(P<0.05),从高到低依次为AIDS、VVC、健康人来源组;小鼠肾脏负荷组间比较,差异无统计学意义(P>0.05)。结论:菌株来源与其致病性强弱有关,菌株的菌丝形成能力是更为重要的致病因素。
Objective: To study on the pathogenicity of three different sources of Candida albicans infection in mice. Method: The mice infected with acquired immunodeficiency syndrome( AIDS),vulvovaginal candidiasis( VVC) patients and healthy persons were selected,and the mice were injected with normal saline as the control group. The death rate and mortality of mice in each group were observed at 7 ~ 11 d,14 ~ 18 d and 21 ~ 25 d respectively. The kidney target organs were isolated by asepsis,and the renal index and kidney load were observed in each group of mice. To collect Candida albicans isolated from kidney,the hyphal formation ability of three strains of Candida albicans from different sources was compared. Results: There were significant differences in mortality,kidney index and hyphal formation ability of three strains infected with different strains in 28 days( P < 0. 05). The order from high to low is AIDS,VVC and the source group of healthy people. There was no significant difference with comparison of renal load groups in mice( P > 0. 05). Conclutions: The mycelium formation ability of the strain is a more important pathogenic factor because of its strong or weak pathogenicity.
Objective: To study on the pathogenicity of three different sources of Candida albicans infection in mice. Method: The mice infected with acquired immunodeficiency syndrome( AIDS),vulvovaginal candidiasis( VVC) patients and healthy persons were selected,and the mice were injected with normal saline as the control group. The death rate and mortality of mice in each group were observed at 7 ~ 11 d,14 ~ 18 d and 21 ~ 25 d respectively. The kidney target organs were isolated by asepsis,and the renal index and kidney load were observed in each group of mice. To collect Candida albicans isolated from kidney,the hyphal formation ability of three strains of Candida albicans from different sources was compared. Results: There were significant differences in mortality,kidney index and hyphal formation ability of three strains infected with different strains in 28 days( P < 0. 05). The order from high to low is AIDS,VVC and the source group of healthy people. There was no significant difference with comparison of renal load groups in mice( P > 0. 05). Conclutions: The mycelium formation ability of the strain is a more important pathogenic factor because of its strong or weak pathogenicity.
引文
[1]HOFS S,MOGAVERO S,HUBE B.Interaction of Candida albicans,with host cells:virulence factors,host defense,escape strategies,and the microbiota[J].J Microbiol,2016,54(3):149.
[2]KIM J Y.Human fungal pathogens:Why should we learn?[J].J Microbiol,2016,54(3):145.
[3]SARDI J C O,SCORZONI L,BERNARDI T,et al.Candida species:current epidemiology,pathogenicity,biofilm formation,natural antifungal products and new therapeutic options[J].J Med Microbiol,2013,62(1):10-24.
[4]MARTINS N,FERREIRA I C,BARROS L,et al.Candidiasis:predisposing factors,prevention,diagnosis and alternative treatment[J].Mycopathologia,2014,177(5-6):223-240.
[5]GUPTA R,MALIK A,RIZVI M,et al.An alarming increase of fungal infections in ntensive care unit:Challenges in the diagnosis and treatment[J].J Appl Pharm Sci,2016,6(11):114-119.
[6]DESAI P R,VAN W L,KURTZ D,et al.Hypoxia and temperature regulated morphogenesis in Candida albicans[J].Plos Genet,2015,11(8):e1005447.
[7]潘丽娜,曹承俊,魏羽佳,等.白念珠菌形态转换及其调控机制的研究进展[J].中国细胞生物学学报,2016,38(9):1049-1059.
[8]SUDBERY P E.Growth of Candida albicans hyphae[J].Nat Rev Microbiol,2011,9(10):737-748.
[9]PHAN Q T,MYERS C L,FU Y,et al.Als3 Is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells[J].Plos Biology,2007,5(3):543-557.
[10]MACCALLUM D M,ODDS F C.Temporal events in the intravenous challenge model for experimental Candida albicans infections in female mice[J].Mycoses,2005,48(3):151-161.
[11]JACOBSEN I D,LüTTICH A,KURZAI O,et al.In vivo imaging of disseminated murine Candida albicans infection reveals unexpected host sites of fungal persistence during antifungal therapy[J].J Antimicrob Chemoth,2014,69(10):2785-2796.
[12]马贤德,雷萍,关洪全.用肾指数评价药物对系统性白色念珠菌感染模型疗效实验研究[J].辽宁中医药大学学报,2014,16(2):140-141.
[13]王梅竹,曹煜,赵亮,等.贵州地区临床酵母菌种类组成和分布[J].山东大学学报:医学版.2014,52(8):94-97.
[14]PRITCHARD M F,JACK A,POWELL L C,et al.Alginate oligosaccharides modify hyphal infiltration of Candida albicans in an in vitro model of invasive human candidosis[J].J Appl Microbiol,2017,123(3):625-636.
[15]FOTOS P G,HELLSTEIN J W.Candida and candidosis.Epidemiology,diagnosis and therapeutic management[J].Dental Clinics of North America,1992,36(4):857-878.
[16]NOBLE S M,GIANETTI B A,WITCHLEY J N.Candida albicans cell-type switching and functional plasticity in the mammalian host[J].Nat Rev Microbiol,2017,15(2):96.
[17]SUDBERY PE.Growth of Candida albicans hyphae[J].Nat Rev Microbiol,2011,9(10):737-748.
[18]GOW N A R,VEERDONK F L V D,BROWN A J P,et al.Candida albicans morphogenesis and host defence:discriminating invasion from colonization[J].Nat Rev Microbiol,2012,10(2):112-122.
[19]杜发娅,阎澜,姜远英,等.侵袭性白念珠菌小鼠感染模型的建立与评估[J].西南国防医药,2014,24(7):697-700.
[20]杜浛,朱利泉.高亲和性铁离子渗透酶Ftr1和Ftr2调控白念珠菌生长和形态[J].微生物学报,2015,55(5):579-586.
[2]KIM J Y.Human fungal pathogens:Why should we learn?[J].J Microbiol,2016,54(3):145.
[3]SARDI J C O,SCORZONI L,BERNARDI T,et al.Candida species:current epidemiology,pathogenicity,biofilm formation,natural antifungal products and new therapeutic options[J].J Med Microbiol,2013,62(1):10-24.
[4]MARTINS N,FERREIRA I C,BARROS L,et al.Candidiasis:predisposing factors,prevention,diagnosis and alternative treatment[J].Mycopathologia,2014,177(5-6):223-240.
[5]GUPTA R,MALIK A,RIZVI M,et al.An alarming increase of fungal infections in ntensive care unit:Challenges in the diagnosis and treatment[J].J Appl Pharm Sci,2016,6(11):114-119.
[6]DESAI P R,VAN W L,KURTZ D,et al.Hypoxia and temperature regulated morphogenesis in Candida albicans[J].Plos Genet,2015,11(8):e1005447.
[7]潘丽娜,曹承俊,魏羽佳,等.白念珠菌形态转换及其调控机制的研究进展[J].中国细胞生物学学报,2016,38(9):1049-1059.
[8]SUDBERY P E.Growth of Candida albicans hyphae[J].Nat Rev Microbiol,2011,9(10):737-748.
[9]PHAN Q T,MYERS C L,FU Y,et al.Als3 Is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells[J].Plos Biology,2007,5(3):543-557.
[10]MACCALLUM D M,ODDS F C.Temporal events in the intravenous challenge model for experimental Candida albicans infections in female mice[J].Mycoses,2005,48(3):151-161.
[11]JACOBSEN I D,LüTTICH A,KURZAI O,et al.In vivo imaging of disseminated murine Candida albicans infection reveals unexpected host sites of fungal persistence during antifungal therapy[J].J Antimicrob Chemoth,2014,69(10):2785-2796.
[12]马贤德,雷萍,关洪全.用肾指数评价药物对系统性白色念珠菌感染模型疗效实验研究[J].辽宁中医药大学学报,2014,16(2):140-141.
[13]王梅竹,曹煜,赵亮,等.贵州地区临床酵母菌种类组成和分布[J].山东大学学报:医学版.2014,52(8):94-97.
[14]PRITCHARD M F,JACK A,POWELL L C,et al.Alginate oligosaccharides modify hyphal infiltration of Candida albicans in an in vitro model of invasive human candidosis[J].J Appl Microbiol,2017,123(3):625-636.
[15]FOTOS P G,HELLSTEIN J W.Candida and candidosis.Epidemiology,diagnosis and therapeutic management[J].Dental Clinics of North America,1992,36(4):857-878.
[16]NOBLE S M,GIANETTI B A,WITCHLEY J N.Candida albicans cell-type switching and functional plasticity in the mammalian host[J].Nat Rev Microbiol,2017,15(2):96.
[17]SUDBERY PE.Growth of Candida albicans hyphae[J].Nat Rev Microbiol,2011,9(10):737-748.
[18]GOW N A R,VEERDONK F L V D,BROWN A J P,et al.Candida albicans morphogenesis and host defence:discriminating invasion from colonization[J].Nat Rev Microbiol,2012,10(2):112-122.
[19]杜发娅,阎澜,姜远英,等.侵袭性白念珠菌小鼠感染模型的建立与评估[J].西南国防医药,2014,24(7):697-700.
[20]杜浛,朱利泉.高亲和性铁离子渗透酶Ftr1和Ftr2调控白念珠菌生长和形态[J].微生物学报,2015,55(5):579-586.