锌对水稻幼苗镉积累及抗氧化系统的影响研究
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摘要
为探明不同浓度Zn对Cd处理水稻幼苗生长及抗氧化系统的影响,以9311水稻品种为试验材料,通过水培方法,从水稻幼苗生长、 Zn与Cd积累量、抗氧化系统变化趋势等方面进行研究。结果表明,低浓度的Zn会抑制Cd处理水稻幼苗生物量的增加,并促进幼苗对Zn和Cd的吸收积累; 100μmol/L Zn对Cd处理水稻生物量有显著促进作用(P <0.05),且该处理下的Zn能明显抑制水稻对Cd的吸收;但当Zn浓度达到3.06×103μmol/L时, Zn对Cd处理水稻具有明显的毒害作用。不同浓度Zn处理下,水稻幼苗地上部的SOD活性、 POD活性、 APX活性呈先升高后下降趋势,而CAT活性则受到抑制, MDA含量均呈上升趋势。低浓度Zn与Cd发生协同作用,促进水稻对Zn和Cd的吸收,抑制水稻生长;中等浓度Zn与Cd发生拮抗作用,抑制水稻对Zn、 Cd的吸收,提高SOD、 POD活性,促进水稻生长;但当Zn浓度达到一定值时,虽然Zn与Cd也发生拮抗作用,但仍会对水稻生长产生明显的抑制作用,损伤幼苗抗氧化系统。
引文
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[23]艾伦弘,汪模辉,李鉴伦,等.镉及镉锌交互作用的植物效应[J].广东微量元素科学,2005,12(12):6-11.
[24]覃勇荣,汤丰瑜,严海杰,等.重金属胁迫对任豆种子萌发及幼苗抗氧化酶活性的影响[J].种子,2017,36(10):31-36.
[25]欧阳洁,李斌彬,王嘉玥,等.镉胁迫对垂柳镉、铁、锰的吸收及生理特性的影响[J].天津师范大学学报(自然版),2017,37(3):37-41.
[26]张军,王文科,耿雅妮,等.黑麦草抗氧化酶对Cd、Zn和Pb复合污染的响应[J].农业环境科学学报,2018,37(6):1 117-1 124.
[2]张宝刚.中低浓度镉污染土壤植物强化修复的野外现场研究[D].南京:南京大学,2010.
[3]陈印军,杨俊彦,方琳娜.我国耕地土壤环境质量状况分析[J].中国农业科技导报,2014,16(2):14-18.
[4]ISHIMARU Y,BASHIR K,NISHIZAWA N K.Zn uptake and translocation in rice plants[J].Rice,2011,4(1):21-27.
[5]ARENASLAGO D,Carvalho L C,SANTOS E S,et al.The physiological mechanisms underlying the ability of Cistus monspeliensis L.from Sao Domingos mine to withstand high Zn concentrations in soils[J].Ecotoxicology&Environmental Safety,2016,129:219-227.
[6]MCDEVITT C A,OGUNNIYI A D,VALKOV E,et al.A molecular mechanism for bacterial susceptibility to zinc[J].Plos Pathogens,2011,7(11):1-9.
[7]张标金,罗林广,魏益华,等.锌镉互作对水稻幼苗镉和矿物质积累的影响[J].广东农业科学,2015,42(5):1-6.
[8]YI W,WANG X,CHAO W,et al.Proteomic profiling of the interactions of Cd/Zn in the roots of dwarf polish wheat(Triticum polonicum L.)[J].Frontiers in Plant Science,2016,7:1-11.
[9]SUN Q,WANG X R,DING S M,et al.Effects of interactions between cadmium and zinc on phytochelatin and glutathione production in wheat(Triticum aestivum L.)[J].Environmental Toxicology,2010,20(2):195-201.
[10]MOHAMMAD A,MOHEMAN A.The effects of cadmium and zinc interactions on the accumulation and tissue distribution of cadmium and zinc in tomato(Lycopersicon esculentum Mill.)[J].Archives of Agronomy&Soil Science,2010,56(5):551-561.
[11]CHERIF J,MEDIOUNI C,AMMAR W B,et al.Interactions of zinc and cadmium toxicity in their effects on growth and in antioxidative systems in tomato plants(Solarium lycopersicum)[J].Journal of Environmental Sciences,2011,23(5):837-844.
[12]许萍,陈铭学,牟仁祥,等.ICP-MS混合模式测定植物性农产品中的9种痕量元素[J].分析测试学报,2011,30(10):1 138-1 142.
[13]LIN Y F,AARTS M G M.The molecular mechanism of zinc and cadmium stress response in plants[J].Cellular&Molecular Life Sciences,2012,69(19):3 187-3 206.
[14]CAKMAK I.Possible roles of zinc in protecting plant cells from damage by reactive oxygen species[J].New Phytologist,2010,146(2):185-205.
[15]JIAN Z,KAI C,PANG Y,et al.QTL mapping and candidate gene analysis of ferrous iron and zinc toxicity tolerance at seedling stage in rice by genome-wide association study[J].BMC Genomics,2017,18(1):4-15.
[16]李天哲,陈爱婷,李彩,等.镉胁迫下硅对水稻幼苗生长与生理响应的影响[J].农业环境科学学报,2018,37(6):1 072-1 078.
[17]HART J J,WELCH R M,NORVELL W A,et al.Transport interaction between cadmium and zinc in roots of bread and durum wheat seedlings[J].Physiologia Plantarum,2002,116(1):73-78.
[18]胡坤,喻华,冯文强,等.中微量元素和有益元素对水稻生长和吸收镉的影响[J].生态学报,2010,31(8):2 341-2 348.
[19]陈贵青,张晓璟,徐卫红,等.不同Zn水平下辣椒体内Cd的积累、化学形态及生理特性[J].环境科学,2010,31(7):1 657-1 662.
[20]曲荣辉,张曦,李合莲,等.不同锌水平对低剂量镉在水稻中迁移能力的影响[J].中国生态农业学报,2016,24(4):517-523.
[21]谢盼盼.锌对镉胁迫下水稻生长及镉的迁移累积研究[M].合肥:安徽农业大学,2016.
[22]叶海波,杨肖娥,何冰,等.东南景天对锌镉复合污染的反应及其对锌镉吸收和积累特性的研究[J].农业环境科学学报,2003,22(5):513-518.
[23]艾伦弘,汪模辉,李鉴伦,等.镉及镉锌交互作用的植物效应[J].广东微量元素科学,2005,12(12):6-11.
[24]覃勇荣,汤丰瑜,严海杰,等.重金属胁迫对任豆种子萌发及幼苗抗氧化酶活性的影响[J].种子,2017,36(10):31-36.
[25]欧阳洁,李斌彬,王嘉玥,等.镉胁迫对垂柳镉、铁、锰的吸收及生理特性的影响[J].天津师范大学学报(自然版),2017,37(3):37-41.
[26]张军,王文科,耿雅妮,等.黑麦草抗氧化酶对Cd、Zn和Pb复合污染的响应[J].农业环境科学学报,2018,37(6):1 117-1 124.