阿胶肽-铁螯合物对缺铁性贫血小鼠的初步药效学研究
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摘要
目的以阿胶为原料,经蛋白酶酶解和氯化亚铁(FeCl_2·4H_2O)处理,对阿胶肽-铁螯合物(Fe(Ⅱ)-HCCA)的补血作用进行初步药效学研究。方法采用饲以低铁饲料造成的缺铁性贫血(IDA)小鼠模型,通过检测其血液指标,考察Fe(Ⅱ)-HCCA的补血效果。结果 Fe(Ⅱ)-HCCA能有效地恢复IDA小鼠血液学指标,达到正常水平,可显著增加HGB、HCT、MCV、MCH、CHCM和MCHC的值(P<0.01),降低HDW、RDW值(P<0.01),提示Fe(Ⅱ)-HCCA是IDA小鼠补铁的有效来源。结论阿胶肽-铁螯合液对缺铁性贫血的症状具有改善作用。
Objective The preliminary pharmacodynamics of the Fe(Ⅱ)-hydrolysate of colla corii asini were studied by protease digestion and ferrous chloride(FeCl_2·4 H_2O) treatment.Methods The therapeutic effect of Fe(Ⅱ)-HCCA on blood rehydration was investigated by using a model of mice with iron deficiency anemia(IDA)caused by low iron diet.Results The results showed that Fe(Ⅱ)-HCCA had effective restorative action returning hematological parameters in IDA mices to normal level.The peptide-iron chelate can significantly increase the values of HGB, HCT, MCV, MCH,CHCM and MCHC in blood of mice with iron deficiency anemia(P<0.01),and decrease HDW,RDWvalue(P<0.01).The findings suggest that Fe(Ⅱ)-HCCA is an effective source of iron supplement for IDA mice.Conclusion Fe(Ⅱ)-HCCA can improve the symptoms of iron deficiency anemia.
Objective The preliminary pharmacodynamics of the Fe(Ⅱ)-hydrolysate of colla corii asini were studied by protease digestion and ferrous chloride(FeCl_2·4 H_2O) treatment.Methods The therapeutic effect of Fe(Ⅱ)-HCCA on blood rehydration was investigated by using a model of mice with iron deficiency anemia(IDA)caused by low iron diet.Results The results showed that Fe(Ⅱ)-HCCA had effective restorative action returning hematological parameters in IDA mices to normal level.The peptide-iron chelate can significantly increase the values of HGB, HCT, MCV, MCH,CHCM and MCHC in blood of mice with iron deficiency anemia(P<0.01),and decrease HDW,RDWvalue(P<0.01).The findings suggest that Fe(Ⅱ)-HCCA is an effective source of iron supplement for IDA mice.Conclusion Fe(Ⅱ)-HCCA can improve the symptoms of iron deficiency anemia.
引文
[1] 林萍.甘氨酸螯合铁合成及其理化性质的研究[D].江南大学硕士学位论文,2004.
[2] Han O,Failla M L,Hill A D,et al.Ascorbate offsets the inhibitory effect of inositol phosphates on iron uptake and transport by Caco-2 cells.[J].Proc Soc Exp Biol Med,1995,210(1):50.
[3] Glahn R P,Van Campen D R.Iron uptake is enhanced in Caco-2 cell monolayers by cysteine and reduced cysteinyl glycine[J].J Nutr,1997,127(4):042.
[4] 刘彤华.缺铁性贫血228例临床分析[J].实用临床医学,2012,13(2):22.
[5] Wei Yueping,WANG Peng.journal of aic heng Teachers College Vol,2005,193:3840.
[6] Tanzadehpanah H,Asoodeh A,Chamani J.An antioxidant peptide derived from Ostrich(Struthio camelus) egg white protein hydrolysates[J].Food Res Int,2012,49(1):105.
[7] Garcia MN,Layrisse M.The effect of change in pH on the solubility of iron bis-glycinate chelate and other iron compounds[J].Arch Latinoam Nutr,2001,51(1):35.
[8] 赵静.猪血红蛋白铁螯合肽的制备及性质研究[D].吉林大学硕士学位论文,2016.
[9] 宁程茜.棉籽蛋白及其功能性多肽的制备与特性研究[D].武汉轻工大学硕士学位论文,2016.
[10] 项欢.可口革囊星虫多肽对小鼠学习记忆能力的影响[D].宁波大学硕士学位论文,2014.
[11] 钟明杰.带鱼下脚料蛋白水解螯合物制备及生物特性研究[D].中国海洋大学硕士学位论文,2009.
[12] 陈升军.米蛋白肽及米蛋白肽铁螯合物生产工艺研究[D].南昌大学硕士学位论文,2008.
[2] Han O,Failla M L,Hill A D,et al.Ascorbate offsets the inhibitory effect of inositol phosphates on iron uptake and transport by Caco-2 cells.[J].Proc Soc Exp Biol Med,1995,210(1):50.
[3] Glahn R P,Van Campen D R.Iron uptake is enhanced in Caco-2 cell monolayers by cysteine and reduced cysteinyl glycine[J].J Nutr,1997,127(4):042.
[4] 刘彤华.缺铁性贫血228例临床分析[J].实用临床医学,2012,13(2):22.
[5] Wei Yueping,WANG Peng.journal of aic heng Teachers College Vol,2005,193:3840.
[6] Tanzadehpanah H,Asoodeh A,Chamani J.An antioxidant peptide derived from Ostrich(Struthio camelus) egg white protein hydrolysates[J].Food Res Int,2012,49(1):105.
[7] Garcia MN,Layrisse M.The effect of change in pH on the solubility of iron bis-glycinate chelate and other iron compounds[J].Arch Latinoam Nutr,2001,51(1):35.
[8] 赵静.猪血红蛋白铁螯合肽的制备及性质研究[D].吉林大学硕士学位论文,2016.
[9] 宁程茜.棉籽蛋白及其功能性多肽的制备与特性研究[D].武汉轻工大学硕士学位论文,2016.
[10] 项欢.可口革囊星虫多肽对小鼠学习记忆能力的影响[D].宁波大学硕士学位论文,2014.
[11] 钟明杰.带鱼下脚料蛋白水解螯合物制备及生物特性研究[D].中国海洋大学硕士学位论文,2009.
[12] 陈升军.米蛋白肽及米蛋白肽铁螯合物生产工艺研究[D].南昌大学硕士学位论文,2008.