响应面法优化电磁裂解提取大黄蒽醌类成分
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摘要
目的建立电磁裂解水提大黄蒽醌类成分的方法,通过响应面法优选最佳提取工艺。方法在提取时间、提取次数、液固比单因素试验的基础上,以大黄总蒽醌收率为指标,采用Box-Behnken试验设计原理,优选出大黄总蒽醌电磁裂解提取的最佳工艺参数,并与超声法和煎煮法进行比较。结果最佳工艺条件为:提取时间3.65 min,提取次数3次,液固比13 (mL∶g),总蒽醌成分的收率为17.12mg·g-1,明显高于超声法和煎煮法。结论电磁裂解提取法具有高效快速、省时节能、绿色环保等优点,作为一种新型的中药提取方法,可为大黄的工业化提取提供一种全新的思路。
Objective To extracte anthraquinones from Rheum palmatum L. by electromagnetic cracking and optimize the extraction process by response surface method. Methods Based on three single factor tests, the extraction time, the number of extractions, the liquid-solid ratio, according to the Box-Behnken test design principle, the optimal extraction parameters of total anthraquinones from Rheum palmatum L. were obtained by electromagnetic cracking with the yield of total anthraquinones as the index. The optimum result was compared with that of the ultrasonic extraction method and the decocting method. Results The optimal conditions were:extraction for 3.65 min, 3 times and the liquid-solid ratio at 13(mL∶g). The yield of total anthraquinones was17.12 mg·g~(-1), higher than that of the ultrasonic extraction method and the decocting method. Conclusion The method is rapid and efficient, energy saving, time saving and environment-friendly. It also provides a brandnew technology for the separation, extraction and industrial production of active ingredients.
Objective To extracte anthraquinones from Rheum palmatum L. by electromagnetic cracking and optimize the extraction process by response surface method. Methods Based on three single factor tests, the extraction time, the number of extractions, the liquid-solid ratio, according to the Box-Behnken test design principle, the optimal extraction parameters of total anthraquinones from Rheum palmatum L. were obtained by electromagnetic cracking with the yield of total anthraquinones as the index. The optimum result was compared with that of the ultrasonic extraction method and the decocting method. Results The optimal conditions were:extraction for 3.65 min, 3 times and the liquid-solid ratio at 13(mL∶g). The yield of total anthraquinones was17.12 mg·g~(-1), higher than that of the ultrasonic extraction method and the decocting method. Conclusion The method is rapid and efficient, energy saving, time saving and environment-friendly. It also provides a brandnew technology for the separation, extraction and industrial production of active ingredients.
引文
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[14]李倩倩,王艳,罗旭,等.大豆低聚糖及其降血脂作用研究进展[J].核农学报,2017,31(9):1788-1793.
[15]李景.大豆磷脂的功能特性及应用现状[J].安徽农学通报,2017,23(22):102-104.
[16]Taladrid D,Marín D,Alemán A,et al. Effect of chemical composition and sonication procedure on properties of food-grade soy lecithin liposomes with added glycerol[J].Food Res Int,2017,100:541-550.
[17]张海晖,裘爱泳,刘军海,等.超声技术提取大黄蒽醌类成分[J].中成药,2005,27(9):1075-1078.
[2]王亦君,冯舒涵,程锦堂,等.大黄蒽醌类化学成分和药理作用研究进展[J].中国实验方剂学杂志,2018,24(13):227-233.
[3]傅兴圣,陈菲,刘训红,等.大黄化学成分与药理作用研究新进展[J].中国新药杂志,2011,20(16):1534-1537.
[4]何正显,信玉琼,陈明.大黄的化学成分、药理作用及其在临床急症中的应用[J].中国中医急症,2007,16(2):227-228.
[5]Adptya U,Arvindekar,Galvina R,et al. Assessment of conventional and novel extraction techniques on extraction efficiency of five anthraquinones from Rheum emodi[J]. J Food Sci Technol,2015,52(10):6574-6582.
[6]Zhao LC,Liang J,Li W,et al. The use of response surface methodology to optimize the ultrasound-assisted extraction of five anthraquinones from Rheum palmatum L.[J].Molec Ules,2011,16(7):5928-5937.
[7]Ren GX,Li L,Hu H. Influence of the environmental factors on the accumulation of the bioactive ingredients in Chinese Rhubarb products[J]. PLoS One,2016,11(5):e0154649.
[8]Sun H,Luo G,Chen D,et al. comprehensive and system review for the pharmacological mechanism of action of rhein,an active anthraquinone ingredient[J]. Front Pharmacol,2016,7(1):247.
[9]姚琴,赵茂俊,张利,等.超声辅助双水相提取大黄中蒽醌类成分[J].天然产物研究与开发,2018,30(8):1346-1353.
[10]Arvindekar AU,Laddha KS. An efficient microwave-assisted extraction of anthraquinones from Rheum emodi:Optimisation using RSM,UV and HPLC analysis and antioxidant studies[J]. Ind Crops Prod,2016,83:587-595.
[11]沈德凤,黄金宝,单畔畔,等.应用电化学指纹图谱优化大黄水提取工艺[J].辽宁中医杂志,2010,37(6):1105-1107.
[12]雷鹏,李新中,朱诗塔,等.煎煮方法对生大黄主要成分含量的影响[J].中国医院药学杂志,2010,30(4):283-286.
[13]赖潇潇,毛敏,张勤帅,等.对大黄后下煎煮方法之疑义[J].医学争鸣,2018,9(2):22-25.
[14]李倩倩,王艳,罗旭,等.大豆低聚糖及其降血脂作用研究进展[J].核农学报,2017,31(9):1788-1793.
[15]李景.大豆磷脂的功能特性及应用现状[J].安徽农学通报,2017,23(22):102-104.
[16]Taladrid D,Marín D,Alemán A,et al. Effect of chemical composition and sonication procedure on properties of food-grade soy lecithin liposomes with added glycerol[J].Food Res Int,2017,100:541-550.
[17]张海晖,裘爱泳,刘军海,等.超声技术提取大黄蒽醌类成分[J].中成药,2005,27(9):1075-1078.