四逆汤对心肌梗死后慢性心衰大鼠心功能和氧化应激反应的作用研究
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摘要
目的观察四逆汤对心肌梗死后慢性心衰大鼠心功能和氧化应激反应的影响。方法采用结扎大鼠冠状动脉导致急性心肌梗死制备慢性心衰大鼠动物模型,将造模成功、存活的大鼠随机分为模型组、盐酸贝那普利组、四逆汤高剂量组、四逆汤中剂量组和四逆汤低剂量组,另设一组未行冠脉结扎术的大鼠为假手术组,每组8只。各组予以相应药物或去离子水,连续灌胃8周后行大鼠心脏彩色多普勒超声检查、病理形态学观察,并比较各组大鼠血清超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶、及过氧化氢的含量差异。结果给药8周后,小动物心动超声结果显示:与假手术组比,模型组大鼠的心脏左室收缩末期内径显著升高(P<0.05),左室射血分数、左室短轴收缩率、舒张末期室间隔厚度、收缩末期室间隔厚度显著降低(P<0.05);与模型组比,四逆汤高、中剂量组左室射血分数、左室短轴收缩率、舒张末期室间隔厚度、收缩末期室间隔厚度显著改善(P<0.05),而低剂量四逆汤有改善左室射血分数、左室短轴及收缩率的趋势,但差异没有统计学意义(P>0.05)。HE染色示:给药组的大鼠心肌结构破坏程度较模型组明显改善;Masson染色示:四逆汤可有效降低心肌组织胶原蛋白含量,抑制心衰大鼠心肌纤维化(P<0.05)。结论四逆汤能够改善心梗所致的慢性心衰大鼠的心功能,其机制可能与改善氧化应激状态,提高抗氧化能力,减少氧化损伤相关。
Objective To observe the effects of Chinese Sini decoction(SNT) on cardiac function and oxidative stress in rats with chronic heart failure after myocardial infarction. Methods Rat model of chronic heart failure and of acute myocardial infarction,caused by ligation of rat coronary artery,were prepared. The survivals were randomly divided into model group, Benner Pury group,SNT high dose group, SNT medium dose group and SNT low dose group. Each group has 10 rats.Rats with no coronary artery ligation was adpoted as Sham operation group. Each group were given relative medicine or deionized water. After 8 weeks of gavage,then rats were evaluated by heart color doppler echocardiography and observed by pathomorphology. Then, the differences of serum SOD、MDA、GSH-Px and H_2O_2 levels were compared. Results After 8 weeks of administration, the heart LVIDs of the rats showed that the model group significantly increased(P<0.05), compared with the sham operation group. LVEF%、LVFS%、IVSd and IVSs decreased significantly in the model group(P<0.05). Compared with the model group, LVEF%、LVFS%、IVSd、IVSs(P<0.05) could improve LVEF%、LVFS%、IVSd、IVSs(P<0.05) by SNT of high dose and medium dose. LVEF%、LVFS% trend could also be improved by low dose SNT. But the differences were not statistically significant(P >0.05). Compared with the model group, the myocardial structural damage in the HE staining administration group was improved. Masson staining showed that the SNT could effectively reduce the collagen content of myocardial tissue and inhibit myocardial fibrosis in rats with heart failure(P<0.05). Conclusion The SNT can improve the cardiac function in rats with chronic heart failure. The mechanism may be related to improving oxidative stress, enhancing antioxidant capacity and reducing oxidative damage.
Objective To observe the effects of Chinese Sini decoction(SNT) on cardiac function and oxidative stress in rats with chronic heart failure after myocardial infarction. Methods Rat model of chronic heart failure and of acute myocardial infarction,caused by ligation of rat coronary artery,were prepared. The survivals were randomly divided into model group, Benner Pury group,SNT high dose group, SNT medium dose group and SNT low dose group. Each group has 10 rats.Rats with no coronary artery ligation was adpoted as Sham operation group. Each group were given relative medicine or deionized water. After 8 weeks of gavage,then rats were evaluated by heart color doppler echocardiography and observed by pathomorphology. Then, the differences of serum SOD、MDA、GSH-Px and H_2O_2 levels were compared. Results After 8 weeks of administration, the heart LVIDs of the rats showed that the model group significantly increased(P<0.05), compared with the sham operation group. LVEF%、LVFS%、IVSd and IVSs decreased significantly in the model group(P<0.05). Compared with the model group, LVEF%、LVFS%、IVSd、IVSs(P<0.05) could improve LVEF%、LVFS%、IVSd、IVSs(P<0.05) by SNT of high dose and medium dose. LVEF%、LVFS% trend could also be improved by low dose SNT. But the differences were not statistically significant(P >0.05). Compared with the model group, the myocardial structural damage in the HE staining administration group was improved. Masson staining showed that the SNT could effectively reduce the collagen content of myocardial tissue and inhibit myocardial fibrosis in rats with heart failure(P<0.05). Conclusion The SNT can improve the cardiac function in rats with chronic heart failure. The mechanism may be related to improving oxidative stress, enhancing antioxidant capacity and reducing oxidative damage.
引文
[1] TEVIK K,THURMER H,Husby MI,et al.Nutritional risk is associated with long term mortality in hospitalized patients with chronic heart failure[J].Clin Nutr ESPEN,2016,12:e20-e29.
[2] KOTUR Stevuljevic J,MEMON L,STEFANOVIC A,et al.Correlation of oxidative stress parameters and inflammatory markers in coronary ar-tery disease patients[J].Clin Biochem,2007,40(3-4):181-187.
[3] 李文佳,郭迪,王迪.强心活力方对心肌梗死后慢性心衰大鼠心功能和氧化应激的作用研究[J].成都中医药大学学报,2017,40(3):17-19..
[4] MAACK C,BOHM M.Targeting mitochondrial oxidative stress in heart failure throttling the afterburner[J].J Am Coll Cardiol,2011,58:83-86.
[5] AMIR O,PAZ H,ROGOWSKI O,et al.Serum oxidative stress level correlates with clinical parameters in chronic systolic heart failure patients[J].Clin Cardiol,2009,32(4):199-203.
[6] 阎书彩,田惠玉,连晓芳,等.氧化应激水平变化与慢性心力衰竭严重程度相关性的临床研究[J].河北医药,2010,32(3):314-315.
[7] 万宇,李南夷,郝蔷薇.经方治疗慢性心衰概况[J].实用中医内科杂志,2016,30(2):114-117.
[8] 袁夏.丹参素钠对心力衰竭保护作用及机制的研究[D].上海:第二军医大学,2014.
[9] 杨钰沛.加参优化方对心肌梗死后心衰大鼠心肌纤维化的影响[D].郑州:河南中医药大学,2017.
[10] 吴爱明,赵明镜,张冬梅,等.气血宁对心肌梗死大鼠心脏保护作用机制研究[J].北京中医药,2017,36(5):405-409,481.
[11] 张依娜,冯微微,陈桂英,等.ISO诱导心力衰竭大鼠模型的建立及评价[J].哈尔滨医科大学学报,2013,47(5):410-413.
[12] 马晓燕.彩色多普勒超声在慢性心力衰竭大鼠心功能测定的应用[D].银川:宁夏医学院,2006.
[13] 王蕾,赵明镜,杨涛,等.从心电图和超声心动图相关性分析研究心肌梗死后心衰模型的早期评价和筛选方法[J].中西医结合心脑血管病杂志,2017,15(22):2816-2820.
[14] Q Ji,HMS Kangli,ZMS Lidong,et al.The effect of hydrogen- rich saline on the brain of rats with transient ischemia [J].Journal of Surgical Research,2011,168(1):95-101.
[15] SEDDON M,LOOI YH,Shah AM.Oxidative stress and redox signalling in cardiac hypertrophy and heart failure[J].Heart,2007,93(8):903-907.
[16] 杨辉,吴伟康.四逆汤全方及拆方对心衰大鼠血液动力学影响的实验研究[J].新中医,2001,33(11):75-77.
[17] 孙森凤,姜雪,张颖颖.附子药理作用研究进展[J].山东化工,2017,46(11):65-67.
[18] 张涛,王桂杰,白书阁,等.附子对老年大鼠抗氧化系统影响的实验研究[J].中国老年学杂志,2001,21(2):135-136.
[19] 徐硕,梁晓丽,李琼,等.中药附子的研究进展[J].西北药学杂志,2017,32(2):248-254.
[20] 王文心.干姜的化学、药理及临床应用特点分析[J].中医临床研究,2016,8(6):146-148.
[21] 孙凤娇,李振麟,钱士辉,等.干姜化学成分和药理作用研究进展[J].中国野生植物资源,2015,34(3):34-37.
[22] 王东,代斌.甘草黄酮的某些药理作用研究进展[J].国医论坛,2005,20(3):53-54.
[23] 叶怀义,龚赋岚,尚明,等.甘草黄酮抗衰老作用的研究[J].哈尔滨商业大学学报(自然科学版),2004,21(1):93-94,97.
[24] 姜雪,孙森凤,王悦,等.甘草药理作用研究进展[J].化工时刊,2017,31(7):25-28.
[25] 孙慧兰,吴伟康.四逆汤有效成分不同组合抗心肌缺血再灌注损伤的作用研究[J].中草药,2002,33(4):47-50.
[2] KOTUR Stevuljevic J,MEMON L,STEFANOVIC A,et al.Correlation of oxidative stress parameters and inflammatory markers in coronary ar-tery disease patients[J].Clin Biochem,2007,40(3-4):181-187.
[3] 李文佳,郭迪,王迪.强心活力方对心肌梗死后慢性心衰大鼠心功能和氧化应激的作用研究[J].成都中医药大学学报,2017,40(3):17-19..
[4] MAACK C,BOHM M.Targeting mitochondrial oxidative stress in heart failure throttling the afterburner[J].J Am Coll Cardiol,2011,58:83-86.
[5] AMIR O,PAZ H,ROGOWSKI O,et al.Serum oxidative stress level correlates with clinical parameters in chronic systolic heart failure patients[J].Clin Cardiol,2009,32(4):199-203.
[6] 阎书彩,田惠玉,连晓芳,等.氧化应激水平变化与慢性心力衰竭严重程度相关性的临床研究[J].河北医药,2010,32(3):314-315.
[7] 万宇,李南夷,郝蔷薇.经方治疗慢性心衰概况[J].实用中医内科杂志,2016,30(2):114-117.
[8] 袁夏.丹参素钠对心力衰竭保护作用及机制的研究[D].上海:第二军医大学,2014.
[9] 杨钰沛.加参优化方对心肌梗死后心衰大鼠心肌纤维化的影响[D].郑州:河南中医药大学,2017.
[10] 吴爱明,赵明镜,张冬梅,等.气血宁对心肌梗死大鼠心脏保护作用机制研究[J].北京中医药,2017,36(5):405-409,481.
[11] 张依娜,冯微微,陈桂英,等.ISO诱导心力衰竭大鼠模型的建立及评价[J].哈尔滨医科大学学报,2013,47(5):410-413.
[12] 马晓燕.彩色多普勒超声在慢性心力衰竭大鼠心功能测定的应用[D].银川:宁夏医学院,2006.
[13] 王蕾,赵明镜,杨涛,等.从心电图和超声心动图相关性分析研究心肌梗死后心衰模型的早期评价和筛选方法[J].中西医结合心脑血管病杂志,2017,15(22):2816-2820.
[14] Q Ji,HMS Kangli,ZMS Lidong,et al.The effect of hydrogen- rich saline on the brain of rats with transient ischemia [J].Journal of Surgical Research,2011,168(1):95-101.
[15] SEDDON M,LOOI YH,Shah AM.Oxidative stress and redox signalling in cardiac hypertrophy and heart failure[J].Heart,2007,93(8):903-907.
[16] 杨辉,吴伟康.四逆汤全方及拆方对心衰大鼠血液动力学影响的实验研究[J].新中医,2001,33(11):75-77.
[17] 孙森凤,姜雪,张颖颖.附子药理作用研究进展[J].山东化工,2017,46(11):65-67.
[18] 张涛,王桂杰,白书阁,等.附子对老年大鼠抗氧化系统影响的实验研究[J].中国老年学杂志,2001,21(2):135-136.
[19] 徐硕,梁晓丽,李琼,等.中药附子的研究进展[J].西北药学杂志,2017,32(2):248-254.
[20] 王文心.干姜的化学、药理及临床应用特点分析[J].中医临床研究,2016,8(6):146-148.
[21] 孙凤娇,李振麟,钱士辉,等.干姜化学成分和药理作用研究进展[J].中国野生植物资源,2015,34(3):34-37.
[22] 王东,代斌.甘草黄酮的某些药理作用研究进展[J].国医论坛,2005,20(3):53-54.
[23] 叶怀义,龚赋岚,尚明,等.甘草黄酮抗衰老作用的研究[J].哈尔滨商业大学学报(自然科学版),2004,21(1):93-94,97.
[24] 姜雪,孙森凤,王悦,等.甘草药理作用研究进展[J].化工时刊,2017,31(7):25-28.
[25] 孙慧兰,吴伟康.四逆汤有效成分不同组合抗心肌缺血再灌注损伤的作用研究[J].中草药,2002,33(4):47-50.