温阳消饮法对慢性心力衰竭大鼠血清ET及CGRP水平影响
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摘要
目的:探索温阳消饮法对慢性心力衰竭大鼠血清ET及CGRP水平的影响。方法:从50只健康雄性SD大鼠中随机取10只为空白组,其余均为造模组,造模组采用腹腔注射阿霉素诱导慢性心力衰竭模型,造模后采用超声心动图检测大鼠心功能,将造模成功大鼠随机分为模型组、温阳消饮组、血府逐瘀组、美托洛尔组,每组10只。进行药物干预,4周后观察心肌病理形态学变化,采用ELISA法测定各组大鼠血清ET、CGRP水平。结果:模型组大鼠血清ET及CGRP含量高于空白组(P<0.05),温阳消饮组、血府逐瘀组及美托洛尔组大鼠血清ET含量低于模型组(P<0.05),温阳消饮组大鼠血清CGRP含量明显高于模型组(P<0.01),血府逐瘀组及美托洛尔组大鼠血清ET含量与温阳消饮组比较,差异无统计学意义(P>0.05),血府逐瘀组及美托洛尔组大鼠血清CGRP含量低于温阳消饮组(P<0.05)。结论:温阳消饮方能显著降低阿霉素诱导的CHF模型大鼠血清ET水平,并能显著升高血清CGRP水平。
Objective: To observe the effect of warming Yang-resolving fluid retention method on serum ET and CGRP in chronic heart failure rats. Methods: The 50 healthy adult male SD rats were randomly divided into blank group(10) and CHF group. The rat model of chronic heart failure was reproduced by intraperitoneal injection of adriamycin. After the completion of the model making, echocardiography was used to determine the cardiac function of rats and randomly divided into model group, warming Yang-resolving fluid retention group,Xuefu Zhuyu group and metoprolol group, 10 rats in each group. After four weeks, the pathological morphological changes of the myocardium were observed by light microscope and the serum levels of ET and CGRP were determined by enzyme-linked immunoassay. Results: The model group showed higher ET and CGRP content than the blank group(P<0.05); and the warming Yang-resolving fluid retention group, Xuefu Zhuyu group and metoprolol group showed lower ET content than model group(P<0.05). The warming Yang-resolving fluid retention group showed higher CGRP content than model group(P<0.01); There was no significant difference on ET content among Xuefu Zhuyu group, metopolol group and warming Yang-resolving fluid retention group(P>0.05);Xuefu Zhuyu group and metopolol group showed lower CGRP content than warming Yang-resolving fluid retention group(P<0.01). Conclusion: The warming Yang-resolving fluid retention method can decrease serum ET level and increase the serum CGRP level in CHF model rats.
Objective: To observe the effect of warming Yang-resolving fluid retention method on serum ET and CGRP in chronic heart failure rats. Methods: The 50 healthy adult male SD rats were randomly divided into blank group(10) and CHF group. The rat model of chronic heart failure was reproduced by intraperitoneal injection of adriamycin. After the completion of the model making, echocardiography was used to determine the cardiac function of rats and randomly divided into model group, warming Yang-resolving fluid retention group,Xuefu Zhuyu group and metoprolol group, 10 rats in each group. After four weeks, the pathological morphological changes of the myocardium were observed by light microscope and the serum levels of ET and CGRP were determined by enzyme-linked immunoassay. Results: The model group showed higher ET and CGRP content than the blank group(P<0.05); and the warming Yang-resolving fluid retention group, Xuefu Zhuyu group and metoprolol group showed lower ET content than model group(P<0.05). The warming Yang-resolving fluid retention group showed higher CGRP content than model group(P<0.01); There was no significant difference on ET content among Xuefu Zhuyu group, metopolol group and warming Yang-resolving fluid retention group(P>0.05);Xuefu Zhuyu group and metopolol group showed lower CGRP content than warming Yang-resolving fluid retention group(P<0.01). Conclusion: The warming Yang-resolving fluid retention method can decrease serum ET level and increase the serum CGRP level in CHF model rats.
引文
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[13]冠心病中医临床研究联盟,中国中西医结合学会心血管疾病专业委员会,中华中医药学会心病分会,等.慢性心力衰竭中医诊疗专家共识[J].中医杂志,2014,55(14):1258-1260.
[14]Giannessi D,Del R S,Vitale R L,et al.The role of endothelins and their receptors in heart failure[J].Pharmacological Research,2001,43(2):111-126.
[15]欧叶涛,扈清云,李梅秀,等.慢性心力衰竭时心肌组织中CGRP的含量变化及mRNA表达[J].解剖学研究,2006(1):38-40.
[2]徐建虎,张琦,杨子庆,等.苓桂术甘汤合方对心衰大鼠脑钠肽、血管紧张素Ⅱ的影响[J].时珍国医国药,2016,27(6):1284-1285.
[3]李玉玲,杨建业,唐俊明,等.阿霉素诱导大鼠心衰模型不同方案的比较[J].中国比较医学杂志,2006(2):93-96,127.
[4]高妍,高建平.心力衰竭动物模型的研究进展[J].中成药,2014,36(1):148-152.
[5]Goldenthal M J.Mitochondrial involvement in myocyte death and heart failure[J].Heart Fail Rev,2016,21(2):137-155.
[6]唐云,郭志华,李雅,等.益心泰颗粒对慢性心衰兔超声心动图指标及血清脑钠肽的影响[J].中成药,2015,37(7):1565-1567.
[7]尚莉莉,全爱君,王馨,等.祛痰通阳汤对慢性心力衰竭模型大鼠超声心动图及血清AngⅡ含量、心肌组织AT1 mR-NA表达的影响[J].中医杂志,2018,59(1):56-60.
[8]乔玉冰,向东,肖应东.苓桂术甘汤结合参麦注射液治疗慢性心力衰竭临床观察[J].中医药导报,2015,21(3):83-85.
[9]江苏新医学院.中药大词典(下册)[M].上海:上海科学技术出版社,2004,2319.
[10]赵兴胜.慢性充血性心力衰竭的现代治疗与展望[J].医学综述.2002,8(2):88-90.
[11]Jenny A D,Paul P G,Eva P,et al.A systematic review of the diagnostic accuracy of natriuretic peptides for heart failure[J].Arch Intem Med,2004,164(18):1978-1984.
[12]万婷,郭书文,吴佳妮,等.益气活血方对情绪应激情况下心肌缺血模型大鼠血清P物质、ET-1及NO含量的影响[J].中医药导报,2015,21(18):23-26.
[13]冠心病中医临床研究联盟,中国中西医结合学会心血管疾病专业委员会,中华中医药学会心病分会,等.慢性心力衰竭中医诊疗专家共识[J].中医杂志,2014,55(14):1258-1260.
[14]Giannessi D,Del R S,Vitale R L,et al.The role of endothelins and their receptors in heart failure[J].Pharmacological Research,2001,43(2):111-126.
[15]欧叶涛,扈清云,李梅秀,等.慢性心力衰竭时心肌组织中CGRP的含量变化及mRNA表达[J].解剖学研究,2006(1):38-40.