不同强度运动训练对脾气虚质大鼠免疫学指标的影响研究
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摘要
目的观察不同强度运动训练对脾气虚质大鼠免疫学指标的影响。方法将48只SD大鼠随机分为A组(脾气虚质)和B组(对照),对A组大鼠进行造模,造模成功后再将其随机分为A1组(小强度运动训练)、A2组(大强度运动训练)及A3组(无运动训练),同时将正常饲养的B组大鼠随机分为B1组(小强度运动训练)、B2组(大强度运动训练)及B3组(无运动训练),每组各8只。运动训练结束后,比较各亚组大鼠白介素-1β(interleukin-1β,IL-1β)、免疫球蛋白(immune globulin,Ig)G、IgA、IgM、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、环鸟苷酸(cyclic guanosine monophosphate,cGMP)、环腺苷酸(cyclic adenosine monophosphate,cAMP)水平及白细胞计数、淋巴细胞计数、淋巴细胞百分比。结果 A组大鼠IgA、IgG、cGMP水平及白细胞计数、淋巴细胞计数、淋巴细胞百分比均显著低于B组(P_均<0.05),IL-1β和TNF-α水平均显著高于B组(P_均<0.05)。A1组大鼠IgA、cGMP水平均显著高于A2组和A3组(P_均<0.05),A2组大鼠IgA水平显著低于A3组(P <0.05);A1组大鼠IgG水平显著高于A2组(P <0.05);A1组和A2组大鼠IL-1β水平均显著低于A3组(P_均<0.05),A2组大鼠TNF-α和cAMP水平均显著高于A1组和A3组(P_均<0.05);A2组大鼠淋巴细胞百分比显著低于A3组(P <0.05)。B2组大鼠淋巴细胞百分比显著低于B1组和B3组(P_均<0.05)。A1组大鼠cAMP水平、白细胞计数、淋巴细胞计数、淋巴细胞百分比均显著低于B1组(P_均<0.05)。A2组大鼠IgA、IgG水平、白细胞计数、淋巴细胞计数均显著低于B2组(P_均<0.05),IL-1β、TNF-α、cAMP水平及cAMP/cGMP比值均显著高于B2组(P_均<0.05)。A3组大鼠IgA、IgG、cGMP水平及白细胞计数、淋巴细胞计数、淋巴细胞百分比均显著低于B3组(P_均<0.05),IL-1β和TNF-α水平均显著高于B3组(P_均<0.05)。结论小强度运动训练可以改善脾气虚质大鼠的免疫功能,从而调节体质;大强度运动训练不利于脾气虚质大鼠免疫功能的恢复,可能损害机体健康。
Objective To observe the effect of different intensity exercise training on immunological indexes of rats with spleen qi deficiency. Method 48 SD rats were randomly divided into group A(spleen qi deficiency) and group B(control), the rats in group A were randomly divided into group A1(low intensity exercise training), group A2(high intensity exercise training) and group A3(no exercise training), at the same time, the rats in group B were randomly divided into group B1(low intensity exercise training), group B2(high intensity exercise training) and group B3(no exercise training), with 8 rats in each group. At the end of training, the levels of interleukin-1β(IL-1β), IgG, IgA, IgM, tumor necrosis factor-α(TNF-α), cyclic guanosine monophosphate(cGMP), cyclic adenosine monop hosphate(cAMP), white blood cell count, lymphocyte count, lymphocyte percentage of the two groups were compared. Result The levels of IgA, IgG, cGMP, white blood cell count, lymphocyte count and lymphocyte percentage in group A were significantly lower than those in group B(P_(all)< 0.05), while the levels of IL-1β and TNF-α in group A were significantly higher than those in group B(P_(all)< 0.05). The levels of IgA and cGMP in group A1 were significantly higher than those in group A2 and group A3(P_(all)< 0.05), the level of IgA in group A2 was significantly lower than that in group A3(P < 0.05), the level of IgG in group A1 was significantly higher than that in group A2(P < 0.05), the levels of IL-1β in group A1 and group A2 were significantly lower than those in group A3(P_(all)<0.05), while the levels of TNF-α and cAMP in group A2 were significantly higher than those in group A1 and group A3(P_(all)<0.05), lymphocyte percentage in group A2 was significantly lower than that in group A3(P < 0.05). The lymphocyte percentage in group B2 was significantly lower than that in group B1 and group B3(P_(all)< 0.05). The level of cAMP, white blood cell count, lymphocyte count and lymphocyte percentage in group A1 were significantly lower than those in group B1(P_(all)< 0.05). The levels of IgA, IgG, white blood cell count and lymphocyte count in group A2 were significantly lower than those in group B2(P_(all)< 0.05), while the levels of IL-1β, TNF-α, cAMP and cAMP/cGMP ratio in group A2 were significantly higher than those in group B2(P_(all)< 0.05). The levels of IgA, IgG, cGMP, white blood cell count and lymphocyte percentage in group A3 were significantly lower than those in group B3(P_(all)< 0.05), while the levels of IL-1β and TNF-α in group A3 were significantly higher than those in group B3(P_(all)< 0.05). Conclusion Low intensity exercise training can improve the immune function of rats with spleen qi deficiency, so as to regulate physique; high intensity exercise training is not conducive to the recovery of immune function of rats with spleen qi deficiency, and may damage the health of the body.
Objective To observe the effect of different intensity exercise training on immunological indexes of rats with spleen qi deficiency. Method 48 SD rats were randomly divided into group A(spleen qi deficiency) and group B(control), the rats in group A were randomly divided into group A1(low intensity exercise training), group A2(high intensity exercise training) and group A3(no exercise training), at the same time, the rats in group B were randomly divided into group B1(low intensity exercise training), group B2(high intensity exercise training) and group B3(no exercise training), with 8 rats in each group. At the end of training, the levels of interleukin-1β(IL-1β), IgG, IgA, IgM, tumor necrosis factor-α(TNF-α), cyclic guanosine monophosphate(cGMP), cyclic adenosine monop hosphate(cAMP), white blood cell count, lymphocyte count, lymphocyte percentage of the two groups were compared. Result The levels of IgA, IgG, cGMP, white blood cell count, lymphocyte count and lymphocyte percentage in group A were significantly lower than those in group B(P_(all)< 0.05), while the levels of IL-1β and TNF-α in group A were significantly higher than those in group B(P_(all)< 0.05). The levels of IgA and cGMP in group A1 were significantly higher than those in group A2 and group A3(P_(all)< 0.05), the level of IgA in group A2 was significantly lower than that in group A3(P < 0.05), the level of IgG in group A1 was significantly higher than that in group A2(P < 0.05), the levels of IL-1β in group A1 and group A2 were significantly lower than those in group A3(P_(all)<0.05), while the levels of TNF-α and cAMP in group A2 were significantly higher than those in group A1 and group A3(P_(all)<0.05), lymphocyte percentage in group A2 was significantly lower than that in group A3(P < 0.05). The lymphocyte percentage in group B2 was significantly lower than that in group B1 and group B3(P_(all)< 0.05). The level of cAMP, white blood cell count, lymphocyte count and lymphocyte percentage in group A1 were significantly lower than those in group B1(P_(all)< 0.05). The levels of IgA, IgG, white blood cell count and lymphocyte count in group A2 were significantly lower than those in group B2(P_(all)< 0.05), while the levels of IL-1β, TNF-α, cAMP and cAMP/cGMP ratio in group A2 were significantly higher than those in group B2(P_(all)< 0.05). The levels of IgA, IgG, cGMP, white blood cell count and lymphocyte percentage in group A3 were significantly lower than those in group B3(P_(all)< 0.05), while the levels of IL-1β and TNF-α in group A3 were significantly higher than those in group B3(P_(all)< 0.05). Conclusion Low intensity exercise training can improve the immune function of rats with spleen qi deficiency, so as to regulate physique; high intensity exercise training is not conducive to the recovery of immune function of rats with spleen qi deficiency, and may damage the health of the body.
引文
[1]王琦.中医体质学[M].北京:人民卫生出版社,2009:48-54.
[2]王琦.9种基本中医体质类型的分类及其诊断表述依据[J].北京中医药大学学报,2005,28(4):1-8.
[3]陈小野,邹世洁,张智,等.大鼠长期脾虚造模的实验研究[J].中国中医基础医学杂志,1995,1(1):37-41.
[4]宋红,郑小伟,王颖.多因素复合建立脾气虚证大鼠模型及以方测证研究[J].浙江中医杂志,2008,43(3):136-138.
[5]高丕明,罗小兵,虞亚明,等.气虚体质大鼠模型的实验研究[J].中国血液流变学杂志,2017,27(4):367-370.
[6]徐文,高丕明,虞亚明,等.脾气虚质大鼠模型免疫学指标研究[J].中国血液流变学杂志,2018,28(2):135-137.
[7]王宏.运动调节机体免疫微环境的研究进展[J].临床输血与检验,2017,19(3):306-308.
[8]Bedford TG,Tipton CM,Wilson NC,et al.Maximum oxygen consumption of rats and its changes with various experimental procedures[J].J Appl Physiol,1979,47(6):1278-1283.
[9]邹明,高丕明,虞亚明,等.不同强度运动训练对气虚体质大鼠血液流变特性的研究[J].中国血液流变学杂志,2017,27(1):89-92.
[10]马露,王俊峰.基于cAMP、cGMP分布和比值探析六经辨证阴阳量化指标[J].中医临床研究,2017,9(27):4-6.
[11]陈春叶,范莹,季刚,等.脓毒症患者血浆白介素-33的变化规律及其临床价值[J].中国医学前沿杂志(电子版),2016,8(11):117-120.
[12]宋歌,吕月涛,狄林林,等.早期肠内营养对重症肺炎大鼠免疫功能的影响分析[J].中国医学前沿杂志(电子版),2017,9(1):69-72.
[13]黄翔.免疫球蛋白联合甲基泼尼松龙对重症支原体肺炎患儿炎性因子与免疫功能的影响[J].中国医刊,2017,52(2):98-100.
[14]陈小云,魏巍,胡子衡.小儿支气管肺炎康复期温振英中医体质分型(平和型/气虚型/阴虚型)免疫特点[J].实用中医内科杂志,2017,31(7):6-8.
[15]Peake JM,Neubauer O,Walsh NP,et al.Recovery of the immune system after exercise[J].J Appl Physiol,2017,122(5):1077-1087.
[16]Simpson RJ,Kunz H,Agha N,et al.Exercise and the Regulation of Immune Functions[J].Prog Mol Biol Transl Sci,2015,135:355-380.
[17]Borghi SM,Zarpelon AC,Pinho-Ribeiro FA,et al.Role of TNF-α/TNFR1 in intense acute swimming-induced delayed onset muscle soreness in mice[J].Physiol Behav,2014,128(10):277-287.
[18]覃飞,赵杰修,王松涛,等.运动性免疫抑制及其干预措施研究进展[J].中国运动医学杂志,2018,37(7):615-623.
[19]冯莉,邱鹏飞.高校气虚体质女大学生运动治疗效果研究[J].宁夏医科大学学报,2013,35(7):793-795.
[20]刘雪凯.九种中医体质大学生体育锻炼调查研究[J].体育科技,2014,35(1):123-126.
[2]王琦.9种基本中医体质类型的分类及其诊断表述依据[J].北京中医药大学学报,2005,28(4):1-8.
[3]陈小野,邹世洁,张智,等.大鼠长期脾虚造模的实验研究[J].中国中医基础医学杂志,1995,1(1):37-41.
[4]宋红,郑小伟,王颖.多因素复合建立脾气虚证大鼠模型及以方测证研究[J].浙江中医杂志,2008,43(3):136-138.
[5]高丕明,罗小兵,虞亚明,等.气虚体质大鼠模型的实验研究[J].中国血液流变学杂志,2017,27(4):367-370.
[6]徐文,高丕明,虞亚明,等.脾气虚质大鼠模型免疫学指标研究[J].中国血液流变学杂志,2018,28(2):135-137.
[7]王宏.运动调节机体免疫微环境的研究进展[J].临床输血与检验,2017,19(3):306-308.
[8]Bedford TG,Tipton CM,Wilson NC,et al.Maximum oxygen consumption of rats and its changes with various experimental procedures[J].J Appl Physiol,1979,47(6):1278-1283.
[9]邹明,高丕明,虞亚明,等.不同强度运动训练对气虚体质大鼠血液流变特性的研究[J].中国血液流变学杂志,2017,27(1):89-92.
[10]马露,王俊峰.基于cAMP、cGMP分布和比值探析六经辨证阴阳量化指标[J].中医临床研究,2017,9(27):4-6.
[11]陈春叶,范莹,季刚,等.脓毒症患者血浆白介素-33的变化规律及其临床价值[J].中国医学前沿杂志(电子版),2016,8(11):117-120.
[12]宋歌,吕月涛,狄林林,等.早期肠内营养对重症肺炎大鼠免疫功能的影响分析[J].中国医学前沿杂志(电子版),2017,9(1):69-72.
[13]黄翔.免疫球蛋白联合甲基泼尼松龙对重症支原体肺炎患儿炎性因子与免疫功能的影响[J].中国医刊,2017,52(2):98-100.
[14]陈小云,魏巍,胡子衡.小儿支气管肺炎康复期温振英中医体质分型(平和型/气虚型/阴虚型)免疫特点[J].实用中医内科杂志,2017,31(7):6-8.
[15]Peake JM,Neubauer O,Walsh NP,et al.Recovery of the immune system after exercise[J].J Appl Physiol,2017,122(5):1077-1087.
[16]Simpson RJ,Kunz H,Agha N,et al.Exercise and the Regulation of Immune Functions[J].Prog Mol Biol Transl Sci,2015,135:355-380.
[17]Borghi SM,Zarpelon AC,Pinho-Ribeiro FA,et al.Role of TNF-α/TNFR1 in intense acute swimming-induced delayed onset muscle soreness in mice[J].Physiol Behav,2014,128(10):277-287.
[18]覃飞,赵杰修,王松涛,等.运动性免疫抑制及其干预措施研究进展[J].中国运动医学杂志,2018,37(7):615-623.
[19]冯莉,邱鹏飞.高校气虚体质女大学生运动治疗效果研究[J].宁夏医科大学学报,2013,35(7):793-795.
[20]刘雪凯.九种中医体质大学生体育锻炼调查研究[J].体育科技,2014,35(1):123-126.