癌毒清对肝癌模型小鼠肿瘤组织病理及微血管密度的影响
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摘要
目的:观察癌毒清对肝癌(hepatocellular carcinoma,HCC)模型小鼠肿瘤组织病理及微血管密度(microvessel density,MVD)的影响。方法:将50只小鼠腋下接种H22肝癌细胞株,建立肝癌异位移植瘤模型。将造模成功的小鼠随机分为模型组、癌毒清高剂量组、癌毒清中剂量组、癌毒清低剂量组及氟尿嘧啶组,每组各10只。癌毒清各剂量组给予不同剂量的癌毒清溶液灌胃,氟尿嘧啶组给予氟尿嘧啶腹腔注射,模型组给予等剂量蒸馏水灌胃。给药12 d后,脱颈处死,剥取瘤组织,称取瘤质量,计算抑瘤率和病理组织学检查,免疫组织化学法观察MVD。结果:与模型组比较,癌毒清高剂量组、癌毒清中剂量组、癌毒清低剂量组及氟尿嘧啶组瘤质量降低,以癌毒清高剂量组最为明显,差异具有统计学意义(P <0. 05)。癌毒清高剂量组、癌毒清中剂量组、癌毒清低剂量组抑瘤率分别为50. 91%、35. 25%、20. 10%,氟尿嘧啶组为43. 99%,与模型组比较,差异有统计学意义(P <0. 05)。与模型组比较,癌毒清高剂量组、癌毒清中剂量组、癌毒清低剂量组及氟尿嘧啶组MVD计数降低,以癌毒清高剂量组最为明显,差异具有统计学意义(P <0. 05)。结论:癌毒清的抗癌机制可能是通过降低肿瘤MVD,抑制肝癌微血管生成而实现的。
Objective: To observe the influence of of Yuduqing Granules( ADQG) on tumor histopathology and microvessel density( MVD) in hepatocellular carcinoma model mice. Methods: Fifty mice were subaxillary inoculated with H22 hepatocellular carcinoma cell line to establish the model of heterotopic transplantation of hepatocellular carcinoma. Successful mice were randomly divided into the model group,the high-dose ADQG group,the medium-dose ADQG group,the low-dose ADQG group and the fluorouracil group,with 10 mice in each group. Each dose group of ADQG was given different doses of ADQG solution by gavage,and the fluorouracil group was given intraperitoneal injection of fluorouracil,and the model group was given equal doses of distilled water by gavage. Twelve days after administration,mice were executed by neck peeling. Tumor tissues were taken out and the quality of tumors was weighed. Tumor inhibition rate and histopathological examination were calculated. MVD was observed by immunohistochemical method. Results: Compared with the model group,the tumour quality of the high-dose ADQG group,the middle-dose ADQG group,the low-dose ADQG group and the fluorouracil group decreased,especially in the high-dose ADQG group. All the differences were statistically significant( P < 0. 05). The inhibition rates of high-dose ADQG group,middle-dose ADQG group and low-dose ADQG group were 50. 91%,35. 25%,20. 10% and 43. 99% respectively,which were significantly different from those of model group( P < 0. 05). Compared with the model group,the MVD count of the high-dose ADQG group,the medium-dose ADQG group,the low-dose ADQG group and the fluorouracil group decreased,especially in the high-dose group. All the differences were statistically significant( P < 0. 05). Conclusion: The anticancer mechanism of ADQG may be achieved by reducing MVD and inhibiting angiogenesis of hepatocellular carcinoma.
Objective: To observe the influence of of Yuduqing Granules( ADQG) on tumor histopathology and microvessel density( MVD) in hepatocellular carcinoma model mice. Methods: Fifty mice were subaxillary inoculated with H22 hepatocellular carcinoma cell line to establish the model of heterotopic transplantation of hepatocellular carcinoma. Successful mice were randomly divided into the model group,the high-dose ADQG group,the medium-dose ADQG group,the low-dose ADQG group and the fluorouracil group,with 10 mice in each group. Each dose group of ADQG was given different doses of ADQG solution by gavage,and the fluorouracil group was given intraperitoneal injection of fluorouracil,and the model group was given equal doses of distilled water by gavage. Twelve days after administration,mice were executed by neck peeling. Tumor tissues were taken out and the quality of tumors was weighed. Tumor inhibition rate and histopathological examination were calculated. MVD was observed by immunohistochemical method. Results: Compared with the model group,the tumour quality of the high-dose ADQG group,the middle-dose ADQG group,the low-dose ADQG group and the fluorouracil group decreased,especially in the high-dose ADQG group. All the differences were statistically significant( P < 0. 05). The inhibition rates of high-dose ADQG group,middle-dose ADQG group and low-dose ADQG group were 50. 91%,35. 25%,20. 10% and 43. 99% respectively,which were significantly different from those of model group( P < 0. 05). Compared with the model group,the MVD count of the high-dose ADQG group,the medium-dose ADQG group,the low-dose ADQG group and the fluorouracil group decreased,especially in the high-dose group. All the differences were statistically significant( P < 0. 05). Conclusion: The anticancer mechanism of ADQG may be achieved by reducing MVD and inhibiting angiogenesis of hepatocellular carcinoma.
引文
[1] YANG Y L,LIU L,WANG Y,et al. The prevalence of depressionand anxiety among Chinese adults with cancer:a systematic reviewand metaanalysis[J]. BMC cancer,2013,13(1):393.
[2] YANG G,WANG Y,ZENG Y,et al. Rapid health transition inChina,1990-2010:findings from the Global Burden of DiseaseStudy 2010[J]. Lancet,2013,381(9882):1987-2015.
[3]武伟,李国兰,周洪伟,等.行肝癌根治术的老年肝癌患者临床特点及长期预后研究[J].中国全科医学,2016,19(14):1663-1666.
[4]魏录翠,韦艾凌,唐健,等.癌痛消颗粒调节大鼠移植性肝癌VEGF和MVD表达的实验研究[J].辽宁中医药大学学报,2008,10(8):159-161.
[5]潘磊,陈培丰.清热解毒中药抗肿瘤作用机理研究进展[J].中华中医药学刊,2007,25(3):569-571.
[6]陈明伟. Rg3对人肺腺癌A549细胞及其内源性VEGF的影响[J].四川大学学报(医学版),2006,37(1):60-62.
[7]李剑明,杨和平.姜黄属中药活性成分对人内皮细胞增殖的抑制作用[J].中国组织工程研究,2004,8(22):4539-4541.
[8]徐晓玉,严鹏科,陈刚,等.川芎嗪对小鼠肺癌血管生长和VEGF表达的抑制[J].中国药理学通报,2004,20(2):151-154.
[9] NTELLAS P,PERIVOLIOTIS K,DADOULI K,et al. Microvesseldensity as a surrogate prognostic marker in patients with multiplemyeloma:a meta-analysis[J]. Acta Haematologica,2017,138(2):77-84.
[10] TANIGAWA N,LU C,MITSUI T,et al. Quantitation of sinusoid-like vessels in hepatocellular carcinoma:Its clinical and prognos-tic significance[J]. Hepatology,1997,26(12):1216-1223.
[11] MURAKAMI K,KASAJIMA A,KAWAGISHI N. Microvessel den-sity in hepatocellular carcinoma:Prognostic significance and re-view of the previous published work[J]. Hepatology Research,2015,45(12):1185-1194.
[2] YANG G,WANG Y,ZENG Y,et al. Rapid health transition inChina,1990-2010:findings from the Global Burden of DiseaseStudy 2010[J]. Lancet,2013,381(9882):1987-2015.
[3]武伟,李国兰,周洪伟,等.行肝癌根治术的老年肝癌患者临床特点及长期预后研究[J].中国全科医学,2016,19(14):1663-1666.
[4]魏录翠,韦艾凌,唐健,等.癌痛消颗粒调节大鼠移植性肝癌VEGF和MVD表达的实验研究[J].辽宁中医药大学学报,2008,10(8):159-161.
[5]潘磊,陈培丰.清热解毒中药抗肿瘤作用机理研究进展[J].中华中医药学刊,2007,25(3):569-571.
[6]陈明伟. Rg3对人肺腺癌A549细胞及其内源性VEGF的影响[J].四川大学学报(医学版),2006,37(1):60-62.
[7]李剑明,杨和平.姜黄属中药活性成分对人内皮细胞增殖的抑制作用[J].中国组织工程研究,2004,8(22):4539-4541.
[8]徐晓玉,严鹏科,陈刚,等.川芎嗪对小鼠肺癌血管生长和VEGF表达的抑制[J].中国药理学通报,2004,20(2):151-154.
[9] NTELLAS P,PERIVOLIOTIS K,DADOULI K,et al. Microvesseldensity as a surrogate prognostic marker in patients with multiplemyeloma:a meta-analysis[J]. Acta Haematologica,2017,138(2):77-84.
[10] TANIGAWA N,LU C,MITSUI T,et al. Quantitation of sinusoid-like vessels in hepatocellular carcinoma:Its clinical and prognos-tic significance[J]. Hepatology,1997,26(12):1216-1223.
[11] MURAKAMI K,KASAJIMA A,KAWAGISHI N. Microvessel den-sity in hepatocellular carcinoma:Prognostic significance and re-view of the previous published work[J]. Hepatology Research,2015,45(12):1185-1194.