食用明胶中微量铬含量测定的方法研究
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摘要
为优化食用明胶中微量铬测定方法,通过微波消解,湿法消解,干法灰化等3种前处理方法,采取原子吸收-石墨炉法和电感耦合等离子质谱法测定明胶中铬含量。结果表明,食用明胶经硝酸-双氧水消解体系进行微波密闭消解,采用原子吸收-石墨炉法测定其微量铬,铬在0～16 ng/mL浓度范围内线性关系良好,相关系数r~2=0.999 1,方法检出限为0.01 mg/kg,加标平均回收率98.7%,相对标准偏差1.7%(n=7),质控样测定结果接近其中值。
In order to find a better method for determination of trace chromium in edible gelatin,the content of chromium in gelatin was determined by atomic absorption-graphite furnace method and inductively coupled plasma mass spectrometry through microwave digestion,wet digestion and dry ashing. The results showed that edible gelatin was digested with nitric acid and hydrogen peroxide in a microwave sealed digestion system,and the trace chromium was determined by graphite furnace atomic absorptionspectrometry method. the linear relationship of chromium was good in the concentration range of 0-16 ng/ml,the correlation coefficient r~2 =0.999 1,its detection limit was 0.01 mg/kg,its average recovery rate was 98.7 %,and RSD value was 1.7 %(n=7),the results of quality control material was close to the median.
In order to find a better method for determination of trace chromium in edible gelatin,the content of chromium in gelatin was determined by atomic absorption-graphite furnace method and inductively coupled plasma mass spectrometry through microwave digestion,wet digestion and dry ashing. The results showed that edible gelatin was digested with nitric acid and hydrogen peroxide in a microwave sealed digestion system,and the trace chromium was determined by graphite furnace atomic absorptionspectrometry method. the linear relationship of chromium was good in the concentration range of 0-16 ng/ml,the correlation coefficient r~2 =0.999 1,its detection limit was 0.01 mg/kg,its average recovery rate was 98.7 %,and RSD value was 1.7 %(n=7),the results of quality control material was close to the median.
引文
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[2]Stephen Juma Mulware.Trace elements and carcinogenicity:a subject in review[J].Biotech,2013,3(2):85-96
[3]陆艳.加工使用明胶的食品中铬含量的分析[J].食品工业,2013,34(7):215-218
[4]Lou Jianlin,Wang Yu,Yao Chunji,et al.Role of DNA Methylation in Cell Cycle Arrest Induced by Cr(VI)in Two Cell Lines[J].Plos One,2013,8(8):1-2
[5]周昆.石墨炉法测定食用明胶铬时三种前处理的比较[J].食品工业,2015,36(11):215-217
[6]赫书东,曹鹏,李雪芝,等.原子荧光光谱法测定食品中碘、铬的应用[J].中国卫生产业,2017,14(26):36-37
[7]冯先进,屈太原.电感耦合等离子体质谱法(ICP-MS)最新应用进展[J].中国无机分析化学,2011,1(1):46-52
[8]田佩瑶,刘丽萍.石墨炉原子吸收法测定矿泉水中总铬[J].中国卫生检验杂质,2005,15(3):308-309
[9]张昂,王飞,钟亚莉,等.微量乙醇对59种无机元素ICP-MS行为的影响[J].分析试验室,2018,37(3):275-279
[10]中华人民共和国国家卫生和计划生育委员会.GB 5009.123-2014食品安全国家标准食品中铬的测定[S].北京:中国标准出版社,2015:1-4
[11]中华人民共和国国家卫生和计划生育委员会,中华人民共和国国家食品药品监督总局.GB 5009.268-2016食品安全国家标准食品中多元素的测定[S].北京:中国标准出版社,2016:1-5
[12]苏荣,洪欣,王晓飞,等.ICP-MS测量水系沉积物中铬的基体效应[J].现代化工,2016,36(10):189-192
[13]刘志光,肖锡林,张桂华.微波消解石墨炉原子吸收法检测明胶中铬的不确定度分析[J].中国卫生产业出,2016,13(17):96-99
[14]张祯,张为人,孔瑾.火焰原子吸收法测定土壤中铬前处理方法的研究[C].北京:中国环境科学学会学术年会论文集,2010:1911-1912