施肥对闽楠幼树PSⅡ叶绿素荧光快速光响应曲线的影响
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摘要
以4年生闽楠幼树作为试验材料,研究不同施肥处理对幼树叶绿素荧光参数的影响及其快速光响应曲线差异。结果表明,初始荧光F_o和最大荧光F_m在施肥一季度后即冬季出现先降低后升高趋势,在施肥三季度后即夏季出现先升高后降低的趋势;PSⅡ最大光化学效率(F_v/F_m)和PSⅡ潜在光化学效率(F_v/F_o)均是中度施肥处理优势显著。不同施肥处理下叶绿素荧光参数结果表明,PSⅡ的实际光量子产量Yield、荧光光化学淬灭系数qp随光强的增加呈现下降趋势,而相同光照强度下,冬季随施肥量的增加其Yield值越大,夏季则是中度施肥量样地Yield值较突出;相对电子传递速率ETR、非光化学淬灭系数q N、NPQ随光强增加呈上升趋势,相同光照强度下ETR冬季数据大量施肥处理样地显著高于其他,夏季未施肥样地明显低于施肥样地,非光化学淬灭系数q N、NPQ高温季节在同光照条件下未施肥处理样地明显高于施肥样地。说明施肥会影响闽楠幼树PSⅡ反应中心开放程度和活性,中度施肥对各项荧光参数促进作用明显,而大量施肥在高温季节则会导致PSⅡ反应中心部分关闭产生光抑制现象。
A four-year-old Phoebe bournei saplings as test material,study effects of different fertilization on chlorophyll fluorescence parameters and rapid light curve for saplings.The results showed that the initial fluorescence F_o and the largest F_m present trend of first decreases and then increases after fertilized of a quarter,and the two parameters first increases and then decreases after fertilized of nine months.maximal PSⅡquantum yield(F_v/F_m)and latent PSⅡquantum yield(F_v/F_o)showed the similar trend that maximum present to moderate fertilized.The rapid light curves of chlorophyll fluorescence parameters were also measured at various light intensities.The effective PSⅡquantum Yield and coefficient of photochemical quenching(qp)decreased with the increase of light intensity.Yield increased with the increase of fertilizing amount in winter,Yield with medium fertilizing amount was more significant in summer.Electron transport rate(ETR),coefficient of nonphotochemical quenching(q N)andnon-photochemical quenching coefficient(NPQ)increased with the increase of illumination intensity.ETR of sample with plenty fertilizer overtop others in winter and sample of contrastive plots lower than other samples that been fertilized in summer.q N and NPQ of contrastive plots markedly overtop others with fertilizer in hot season.Results show that fertilizer could affect the openness andactivity of PSⅡreaction center which Phoebe bournei saplings.Moderate fertilizer could promote fluorescence parameters of samples,heavy fertilization made part of PSⅡreaction center closure and then led photo inhibition in hot season.
A four-year-old Phoebe bournei saplings as test material,study effects of different fertilization on chlorophyll fluorescence parameters and rapid light curve for saplings.The results showed that the initial fluorescence F_o and the largest F_m present trend of first decreases and then increases after fertilized of a quarter,and the two parameters first increases and then decreases after fertilized of nine months.maximal PSⅡquantum yield(F_v/F_m)and latent PSⅡquantum yield(F_v/F_o)showed the similar trend that maximum present to moderate fertilized.The rapid light curves of chlorophyll fluorescence parameters were also measured at various light intensities.The effective PSⅡquantum Yield and coefficient of photochemical quenching(qp)decreased with the increase of light intensity.Yield increased with the increase of fertilizing amount in winter,Yield with medium fertilizing amount was more significant in summer.Electron transport rate(ETR),coefficient of nonphotochemical quenching(q N)andnon-photochemical quenching coefficient(NPQ)increased with the increase of illumination intensity.ETR of sample with plenty fertilizer overtop others in winter and sample of contrastive plots lower than other samples that been fertilized in summer.q N and NPQ of contrastive plots markedly overtop others with fertilizer in hot season.Results show that fertilizer could affect the openness andactivity of PSⅡreaction center which Phoebe bournei saplings.Moderate fertilizer could promote fluorescence parameters of samples,heavy fertilization made part of PSⅡreaction center closure and then led photo inhibition in hot season.
引文
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[21]刘宝,陈存及,林达定,等.21个闽楠种源叶片光合色素含量及叶绿素荧光参数分析[J].江西农业大学学报,2014,36(1):115-121.
[22]杨柳青,杨帆,杨琳.不同光照条件对有柄石韦的形态和生理特性的影响[J].经济林研究,2016,34(2):109-113.
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[24]周丽娜.基于叶绿素荧光光谱分析的稻叶瘟病害识别与预警[D].长春:吉林大学,2014.
[25]郭春燕,李晋川,岳建英,等.两种高质牧草不同生育期光合生理日变化及光响应特征[J].生态学报,2013,33(6):1751-1761.
[26]P.H.C.Eilers,J.C.H.Peeters.A model for the relationship between light intensity and the rate of photosynthesis in phytoplankton[J].Ecological Modelling,1988,42(3):199-215.
[27]高培培,章艺,吴玉环,等.外源水杨酸对铝胁迫下栝楼光合特性及耐铝性的影响[J].水土保持学报,2012,26(6):268-273.
[2]曾祥谓.我国多功能森林经营中的珍贵树种问题研究[D].北京:中国林业科学研究院,2010.
[3]刘军,姜景民,陈益泰,等.闽楠种子轻基质容器育苗及优良家系选择[J].西北林学院学报,2011,26(6):70-73.
[4]吴际友,黄明军,陈明皋,等.闽楠种源苗期生长差异与早期选择研究[J].中南林业科技大学学报,2015,35(11):1-4.
[5]张伟.闽楠种子萌发技术与幼苗荧光特性的研究[D].长沙:中南林业科技大学,2013.
[6]朱亚红,孙存举,朱铭强,等.河北杨与沙棘的光合生理特性及叶绿素荧光参数比较[J].中南林业科技大学学报,2015,35(2):26-31.
[7]葛永金,王军峰,方伟,等.闽楠地理分布格局及其气候特征研究[J].江西农业大学学报,2012,34(4):749-753.
[8]邵明晓.不同林龄闽楠人工林生物量与碳密度研究[D].长沙:中南林业科技大学,2014.
[9]葛永金.闽楠栽培生理生态基础研究[D].北京:北京林业大学,2014.
[10]申展.闽楠无性繁殖技术研究[D].长沙:中南林业科技大学,2013.
[11]王振兴,朱锦懋,王健,等.闽楠幼树光合特性及生物量分配对光环境的响应[J].生态学报,2012,32(12):3841-3848.
[12]Salgado-Luarte C,Gianoli E.Herbivory may modify functional responses to shade in seedlings of a light-demanding tree species.Functional Ecology,2011,25(3):492-499.
[13]曹刚.不同LED光质对黄瓜和结球甘蓝苗期生长、光合特性及内源激素的影响[D].兰州:甘肃农业大学,2013.
[14]许楠.黑龙江省桑树氮肥运筹及光合调控机理的研究[D].哈尔滨:东北林业大学,2013.
[15]郝岩松.模拟酸雨对墨西哥柏幼苗光合生理特性的影响[D].南京:南京林业大学,2013.
[16]Bernier M,Popovic R,Carpentier R.Mercury inhibition at the donor side of photosystemⅡis reversed by chloride[J].FEBS Letters,1993,321(1):19-23.
[17]Baszyńki T,Wajda L,Król M,et al.Photosynthetic activities of cadmium-treated tomato plants[J].Physiologia Plantarum,1980,48(3):365-370.
[18]李泽,谭晓风,卢锟,等.根外追肥对油桐幼苗生长、光合作用及叶绿素荧光参数的影响[J].中南林业科技大学学报,2016,36(2):40-44.
[19]Valladares F,Niinemets.Shade tolerance a key plant feature of complex nature and consequences[J].Annual Review of Ecology,Evolution,and Systematics,2008,39(1):237-257.
[20]石元豹,曹兵.CO2浓度倍增对宁夏枸杞叶绿素荧光参数的影响[J].经济林研究,2015,33(3):108-111.
[21]刘宝,陈存及,林达定,等.21个闽楠种源叶片光合色素含量及叶绿素荧光参数分析[J].江西农业大学学报,2014,36(1):115-121.
[22]杨柳青,杨帆,杨琳.不同光照条件对有柄石韦的形态和生理特性的影响[J].经济林研究,2016,34(2):109-113.
[23]Schreibei U,Gademann R,Ralph PJ,et al.Assessment of photosynthetic performance of Prochloron in Lissoclinum patella in hospite by chlorophyll fluorescence measurements[J].Plant Cell and Physiology,1997,38(8):945-951.
[24]周丽娜.基于叶绿素荧光光谱分析的稻叶瘟病害识别与预警[D].长春:吉林大学,2014.
[25]郭春燕,李晋川,岳建英,等.两种高质牧草不同生育期光合生理日变化及光响应特征[J].生态学报,2013,33(6):1751-1761.
[26]P.H.C.Eilers,J.C.H.Peeters.A model for the relationship between light intensity and the rate of photosynthesis in phytoplankton[J].Ecological Modelling,1988,42(3):199-215.
[27]高培培,章艺,吴玉环,等.外源水杨酸对铝胁迫下栝楼光合特性及耐铝性的影响[J].水土保持学报,2012,26(6):268-273.