不同生态条件下玉米单倍体诱导系JS5-27和JS17-1的诱导效果
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摘要
单倍体诱导系JS5-27、JS17-1具有诱导率高、农艺性状优良、抗病性强、花粉量大等特点。以诱导系JS5-27、JS17-1为诱导材料,以JS6-2和外引系农大高诱5号、Stock6为对照,在吉林省长春、桦甸、白城3个不同生态条件下对不同品种类型材料进行了杂交诱导试验,探讨玉米单倍体诱导系JS5-27、JS17-1的诱导效果。结果表明:诱导系JS5-27在3个地点平均诱导率最高(16. 54%),JS17-1次之(15. 62%),显著优于其他诱导系;诱导系JS5-27对杂交种平均诱导率最高,极显著高于自交系和DH系。
Maize haploid induction lines JS5-27 and JS17-1 are of high induction rate,fine agronomic traits,strong resistance to disease and large pollen volume. To study the induction effect of maize,induction lines JS5-27 and JS17-1 were selected,with JS6-2,Stock6 and introduced induction line CAU HI5( High Induction 5) as control. Hybrid induction was carried out on different types of germplasms in Changchun,Baicheng and Huadian,respectively. Results showed that average induction rate in the three ecotypes of JS5-27 was the highest( 16. 54%),and JS17-1 took the second place( 15. 62%),significantly better than other induction lines. Induction line JS5-27 had the highest average induction rate on hybrids,very significantly higher than that of inbred lines and DH lines.
Maize haploid induction lines JS5-27 and JS17-1 are of high induction rate,fine agronomic traits,strong resistance to disease and large pollen volume. To study the induction effect of maize,induction lines JS5-27 and JS17-1 were selected,with JS6-2,Stock6 and introduced induction line CAU HI5( High Induction 5) as control. Hybrid induction was carried out on different types of germplasms in Changchun,Baicheng and Huadian,respectively. Results showed that average induction rate in the three ecotypes of JS5-27 was the highest( 16. 54%),and JS17-1 took the second place( 15. 62%),significantly better than other induction lines. Induction line JS5-27 had the highest average induction rate on hybrids,very significantly higher than that of inbred lines and DH lines.
引文
[1] Geiger H H,Gordillo G A. Doubled haploids in hybrid maize breeding[J]. Maydica,2009,54:485-499.
[2] Bennett M D,Finch R A,Barclay I R. The time rate and mechanism of chromosome elimination in Hordeum hybrids[J].Chromosoma,1976,54:175-200.
[3]孙贵星,杨巍,任雪娇,等.遗传基础和种植方式对玉米单倍体成活率及授粉成功率的影响[J].吉林农业大学学报,2016,38(1):20-25.
[4]王贺,李继竹,张继伟,等.秋水仙素和除草剂浸芽加倍玉米单倍体效率的研究[J].吉林农业大学学报,2013,35(4):384-388.
[5]才卓,徐国良,刘向辉,等.玉米高频率单倍生殖诱导系吉高诱3号的选育[J].玉米科学,2007,15(1):1-4.
[6]惠国强,杜何为,张铭堂,等.不同除草剂加倍玉米单倍体的效率[J].作物学报,2012,38(3):416-422.
[7]姜龙,慈佳宾,崔学宇,等.不同生态条件下玉米单倍体诱导率和加倍率研究[J].吉林农业大学学报,2014,36(2):139-143.
[8]刘晓鑫,杨巍,慈佳宾,等.不同基础材料和秋水仙素浓度对玉米单倍体加倍率的影响[J].吉林农业大学学报,2015,37(2):136-140.
[9] Seitz G. The use of doubled haploids in corn breeding[C]∥Proceedings of the forty first annual Illinois Corn Breeders'School.USA:Illinois,Urbana-Champaign,2005:1-8.
[10] Rotarenco V A. Production of matroclinous maize haploids following natural and artificial pollination with a haploid inducer[J]. Maize Genet Coop News,2002,76:16.
[11] Sarkar K R,Prasanna B M,Gayen P. Distribution of haploids on the ear[J]. Maize Genet Coop Newslett,1995,69:107.
[12]陈绍江,黎亮.鉴别玉米孤雌生殖单倍体的方法:CN200810223529. 4[P].2008-10-06.
[13]陈绍江,黎亮,李浩川.玉米单倍体育种技术[M].北京:中国农业大学出版社,2009.
[14] Chase S S. Production of homozygous diploids of maize from monoploids[J]. Agron,1952,44:263-267.
[15] Chase SS. Monoploids and diploids of maize:a comparison of genotypic equivalents[J]. American Journal of Botany,1964,51:928-933.
[16] Coe E H. Aline of maize with high haploid frequency[J]. The American Naturalist,1959,93:381-382.
[17]杨巍,任雪娇,崔学宇,等.玉米单倍体诱导系JS6-2诱导效果的研究[J].西北农林科技大学学报(自然科学版),2015(7):89-94.
[18] Rober F K,Gordillo G A,Geiger H H. In vivo haploid induction in maize-performance of new inducers and significance for doubled haploid lines in hybrid breeding[J]. Maydica,2005,50:275-283.
[19]刘志增,宋同明.玉米高频率孤雌生殖单倍体诱导系的选育与鉴定[J].玉米科学,2000,26(5):570-574.
[20]刘晓鑫,慈佳宾,崔学宇,等.玉米孤雌生殖单倍体高效诱导研究[J].西北农林科技大学(自然科学版),2015,43(4):35-42.
[21]李国良,苏俊,李春霞,等.农大高诱1号对玉米不同种质和世代单倍体诱导频率的研究[J].玉米科学,2008,16(5):3-6.
[2] Bennett M D,Finch R A,Barclay I R. The time rate and mechanism of chromosome elimination in Hordeum hybrids[J].Chromosoma,1976,54:175-200.
[3]孙贵星,杨巍,任雪娇,等.遗传基础和种植方式对玉米单倍体成活率及授粉成功率的影响[J].吉林农业大学学报,2016,38(1):20-25.
[4]王贺,李继竹,张继伟,等.秋水仙素和除草剂浸芽加倍玉米单倍体效率的研究[J].吉林农业大学学报,2013,35(4):384-388.
[5]才卓,徐国良,刘向辉,等.玉米高频率单倍生殖诱导系吉高诱3号的选育[J].玉米科学,2007,15(1):1-4.
[6]惠国强,杜何为,张铭堂,等.不同除草剂加倍玉米单倍体的效率[J].作物学报,2012,38(3):416-422.
[7]姜龙,慈佳宾,崔学宇,等.不同生态条件下玉米单倍体诱导率和加倍率研究[J].吉林农业大学学报,2014,36(2):139-143.
[8]刘晓鑫,杨巍,慈佳宾,等.不同基础材料和秋水仙素浓度对玉米单倍体加倍率的影响[J].吉林农业大学学报,2015,37(2):136-140.
[9] Seitz G. The use of doubled haploids in corn breeding[C]∥Proceedings of the forty first annual Illinois Corn Breeders'School.USA:Illinois,Urbana-Champaign,2005:1-8.
[10] Rotarenco V A. Production of matroclinous maize haploids following natural and artificial pollination with a haploid inducer[J]. Maize Genet Coop News,2002,76:16.
[11] Sarkar K R,Prasanna B M,Gayen P. Distribution of haploids on the ear[J]. Maize Genet Coop Newslett,1995,69:107.
[12]陈绍江,黎亮.鉴别玉米孤雌生殖单倍体的方法:CN200810223529. 4[P].2008-10-06.
[13]陈绍江,黎亮,李浩川.玉米单倍体育种技术[M].北京:中国农业大学出版社,2009.
[14] Chase S S. Production of homozygous diploids of maize from monoploids[J]. Agron,1952,44:263-267.
[15] Chase SS. Monoploids and diploids of maize:a comparison of genotypic equivalents[J]. American Journal of Botany,1964,51:928-933.
[16] Coe E H. Aline of maize with high haploid frequency[J]. The American Naturalist,1959,93:381-382.
[17]杨巍,任雪娇,崔学宇,等.玉米单倍体诱导系JS6-2诱导效果的研究[J].西北农林科技大学学报(自然科学版),2015(7):89-94.
[18] Rober F K,Gordillo G A,Geiger H H. In vivo haploid induction in maize-performance of new inducers and significance for doubled haploid lines in hybrid breeding[J]. Maydica,2005,50:275-283.
[19]刘志增,宋同明.玉米高频率孤雌生殖单倍体诱导系的选育与鉴定[J].玉米科学,2000,26(5):570-574.
[20]刘晓鑫,慈佳宾,崔学宇,等.玉米孤雌生殖单倍体高效诱导研究[J].西北农林科技大学(自然科学版),2015,43(4):35-42.
[21]李国良,苏俊,李春霞,等.农大高诱1号对玉米不同种质和世代单倍体诱导频率的研究[J].玉米科学,2008,16(5):3-6.