博落回中血根碱和白屈菜赤碱的稳定性研究
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摘要
采用HPLC法测定了博落回中血根碱和白屈菜赤碱在7种溶剂中的溶解性和稳定性,同时考察了水质、光源、氧化剂、温度、pH值对其稳定性的影响。结果表明,血根碱和白屈菜赤碱在甲醇、乙醇中的溶解性和稳定性较好;血根碱和白屈菜赤碱在蒸馏水、雨水中的稳定性不受光影响,很稳定,而在自来水中无论避光与否都不稳定;氧化剂对血根碱和白屈菜赤碱稳定性影响很大;血根碱和白屈菜赤碱在室温～54℃下稳定;血根碱在pH值2.5～7.0范围内稳定,白屈菜赤碱在pH值2.5～8.0范围内稳定。
We determined the solubility and stability of sanguinarine and chelerythrine in seven kinds of solvents by HPLC,and investigated the effects of water quality,light source,oxidant,temperature,and pH value on the stability of sanguinarine and chelerythrine.The results show that sanguinarine and chelerythrine have good solubility and stability in methanol and ethanol,and sanguinarine and chelerythrine remain stable in distilled water and rainwater,which are not influenced by light but are unstable in running water whether under light or not.Moreover,oxidant has a great effect on the stability of sanguinarine and chelerythrine.Sanguinarine and chelerythrine are stable at room temperature to 54 ℃.Furthermore,sanguinarine is stable when the pH value ranges from 2.5 to 7.0,while chelerythrine is stable when the pH value ranges from 2.5 to 8.0.
We determined the solubility and stability of sanguinarine and chelerythrine in seven kinds of solvents by HPLC,and investigated the effects of water quality,light source,oxidant,temperature,and pH value on the stability of sanguinarine and chelerythrine.The results show that sanguinarine and chelerythrine have good solubility and stability in methanol and ethanol,and sanguinarine and chelerythrine remain stable in distilled water and rainwater,which are not influenced by light but are unstable in running water whether under light or not.Moreover,oxidant has a great effect on the stability of sanguinarine and chelerythrine.Sanguinarine and chelerythrine are stable at room temperature to 54 ℃.Furthermore,sanguinarine is stable when the pH value ranges from 2.5 to 7.0,while chelerythrine is stable when the pH value ranges from 2.5 to 8.0.
引文
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[15] 齐滨,赵大庆,刘莉,等.人参西洋参中超氧化物歧化酶的稳定性研究[J].时珍国医国药,2014,25(2):293-294.QI B,ZHAO D Q,LIU L,et al.Study on stability of superoxide dismutase in ginseng American ginseng[J].Lishizhen Medicine and Materia Medica Research,2014,25(2):293-294.
[16] 谭乐俊,王萌,任晓亮,等.基于UPLC-Q/TOF-MS的中药水蜜丸制剂二至丸多成分稳定性研究[J].中草药,2016,47(13):2260-2268.TAN L J,WANG M,REN X L,et al.Stability of multiple components in Er-Zhi-Wan(water honey pills) by UPLC-Q/TOF-MS[J].Chinese Traditional and Herbal Drugs,2016,47(13):2260-2268.
[17] 边帅,于士婷,白雪媛,等.脑塞通丸中黄芩苷高效液相色谱检测方法的建立及其评价[J].时珍国医国药,2016,27(8):1891-1893.BIAN S,YU S T,BAI X Y,et al.Simultaneous determination of baicalin in Naosaitong pills by HPLC[J].Lishizhen Medicine and Materia Medica Research,2016,27(8):1891-1893.
[2] ZENG J G,LIU Y S,LIU W,et al.Integration of transcriptome,proteome and metabolism data reveals the alkaloids biosynthesis in Macleaya cordata and Macleayamicrocarpa[J].PLoS One,2013,8(1):36-38.
[3] QING Z X,CHENG P,LIU X B,et al.Structural speculation and identification of alkaloids in Macleaya cordata fruits by high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combined with a screening procedure[J].Rapid Communications in Mass Spectrometry,2014,28(9):1033-1044.
[4] 杜先华,黄松,冯泓瑞,等.博落回提取物血根碱对肿瘤细胞增殖及凋亡的影响[J].时珍国医国药,2014,25(12):2860-2861.DU X H,HUANG S,FENG H R,et al.Effect of sanguinarine extracted from Macleaya cordata on proliferation and apoptosis of tumor cells[J].Lishizhen Medicine and Materia Medica Research,2014,25(12):2860-2861.
[5] 冯泓瑞,黄松,陈玲玲,等.白屈菜红碱、血根碱单体的分离和鉴定[J].中国实验方剂学杂志,2013,19(14):36-38.FENG H R,HUANG S,CHEN L L,et al.Isolation and identification of the monomers of chelerythrine and sanguinarine[J].Chinese Journal of Experimental Traditional Medical Formulae,2013,19(14):36-38.
[6] 鲁文静,罗世恒,陈世忠.白屈菜中多种生物碱含量的高效液相色谱法测定[J].时珍国医国药,2013,24(3):601-603.LU W J,LUO S H,CHEN S Z.Determination of alkaloids in celandine by high performance liquid chromatography[J].Lishizhen Medicine and Materia Medica Research,2013,24(3):601-603.
[7] YAO-KOUASSI P A,CARON C,HARISOLO R,et al.New nitro-benzo[c]phenanthridine and indolopyridoquinazoline alkaloids from Zanthoxylum atchoum[J].Comptes Rendus Chimie,2015,18(8):891-897.
[8] 章亮,陈泽慧,陈韩英,等.基于网络药理学的白屈菜抗肿瘤分子机制研究[J].中草药,2018,49(3):646-657.ZHANG L,CHEN Z H,CHEN H Y,et al.Molecular mechanism of anti-tumor of celandine based on network pharmacology[J].Chinese Traditional and Herbal Drugs,2018,49(3):646-657.
[9] QING Z X,CHENG P,LIU X B,et al.Systematic identification of alkaloids in Macleaya microcarpa fruits by liquid chromatography tandem mass spectrometry combined with the isoquinoline alkaloids biosynthetic pathway [J].Journal of Pharmaceutical and Biomedical Analysis,2015,103:26-34.
[10] QING Z X,XU Y Q,YANG P,et al.Mass spectrometry-guide isolation of two new benzoquinoline alkaloids from Macleaya cordata[J].Natural Product Research,2015,30(9):1030-1035.
[11] 杨鹏,邹超兰,向锋,等.博落回中1个新的6,6a-断苯菲啶生物碱[J].中国中药杂志,2016,41(22):4194-4196.YANG P,ZOU C L,XIANG F,et al.A new 6,6a-offbenzo phenanthridine alkaloid from Macleaya cordata[J].China Journal of Chinese Materia Medica,2016,41(22):4194-4196.
[12] 卿志星,徐玉琴,杨鹏,等.博落回果荚中生物碱的研究[J].中药材,2016,39(2):312-314.QING Z X,XU Y Q,YANG P,et al.Studies on the alkaloids in fruit from Macleaya cordata[J].Journal of Chinese Medicinal Materials,2016,39(2):312-314.
[13] ABIZOV E A,TOLKACHEV O N,KOPYLOVA I E,et al.Distribution of the sum of sanguinarine and chelerythrine in the above-ground part of Macleaya microcarpa[J].Pharmaceutical Chemistry Journal,2003,37(8):413-414.
[14] ZHANG F,CHEN B,XIAO S,et al.Optimization and comparison of different extraction techniques for sanguinarine and chelerythrine in fruits of Macleaya cordata(Willd) R.Br.[J].Separation and Purification Technology,2005,42(3):283-290.
[15] 齐滨,赵大庆,刘莉,等.人参西洋参中超氧化物歧化酶的稳定性研究[J].时珍国医国药,2014,25(2):293-294.QI B,ZHAO D Q,LIU L,et al.Study on stability of superoxide dismutase in ginseng American ginseng[J].Lishizhen Medicine and Materia Medica Research,2014,25(2):293-294.
[16] 谭乐俊,王萌,任晓亮,等.基于UPLC-Q/TOF-MS的中药水蜜丸制剂二至丸多成分稳定性研究[J].中草药,2016,47(13):2260-2268.TAN L J,WANG M,REN X L,et al.Stability of multiple components in Er-Zhi-Wan(water honey pills) by UPLC-Q/TOF-MS[J].Chinese Traditional and Herbal Drugs,2016,47(13):2260-2268.
[17] 边帅,于士婷,白雪媛,等.脑塞通丸中黄芩苷高效液相色谱检测方法的建立及其评价[J].时珍国医国药,2016,27(8):1891-1893.BIAN S,YU S T,BAI X Y,et al.Simultaneous determination of baicalin in Naosaitong pills by HPLC[J].Lishizhen Medicine and Materia Medica Research,2016,27(8):1891-1893.