反射式离轴数字全息显微光强参数研究
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摘要
为了探究光强参数对于反射式离轴数字全息显微再现像质量的影响,概述了数字全息显微技术的研究现状以及影响全息图再现像质量的因素,基于菲涅尔衍射积分,论述了离轴数字全息图的记录与再现像过程,分析了一定记录距离下物光和参考光光强比对于全息图再现像质量的影响。通过控制物光路以及参考光路中滤光片的滤光能力来实现不同的物参光强比,从而记录下标准分辨率板的反射式显微全息图并进行重构。其实验结果表明:在记录距离已知的情况下,物光和参考光的光强比对反射式显微全息图再现像质量有着显著影响。因此在保证其他实验条件不变的情况下,确定适当的物参光强比是提高全息图质量的关键。通过理论分析,确定了实验的记录距离,并对该记录距离下的频谱图进行分析,验证记录距离的正确性。在相同记录距离下进行不同物参光强比下的对比实验,确定了可以实现较好重构效果的物参光强比范围。实验结果亦为进一步研究基于反射式数字全息的三维重构提供了理论依据与技术条件。
This paper summarizes the research status of digital holography microscopy technology and factors affecting the quality of the reconstructed image of hologram in order to investigate the influence of object light intensity parameters on the quality of reconstructed image of reflective off-axis digital holography. Based on Finel diffraction integral,the process of recording and reproducing the off-axis digital hologram is discussed. The influence of object light intensity and reference light intensity ratio on the reconstructed image quality of hologram is analyzed. The standard resolution plate is the research object.By controlling the filtering capability of filter in the object light and reference light path,we can achieve different light intensity ratio of material reference,and record the reflection hologram of standard resolution plate and reconstruct it. The experimental results show that the object and reference light intensity ratio have a significant impact on the quality of hologram recurrent image when the recording distance is known. Therefore,it's the key to determining an appropriate recording distance and the object and reference light intensity ratio for improving the quality of hologram under the condition that other experimental conditions remain unchanged. Through theoretical analysis,the recording distance of the experiment is determined,and the spectrum chart under the recording distance is analyzed to verify the correctness of the recording distance. Under the same record distance,the contrast experiment of light intensity ratio of different material reference is carried out,and the range of light intensity ratio of material reference which can achieve better reconstruction effect is determined. The experimental result will provide theoretical basis and technical conditions for studying the reflective digital holography based on three-dimensional reconstruction.
This paper summarizes the research status of digital holography microscopy technology and factors affecting the quality of the reconstructed image of hologram in order to investigate the influence of object light intensity parameters on the quality of reconstructed image of reflective off-axis digital holography. Based on Finel diffraction integral,the process of recording and reproducing the off-axis digital hologram is discussed. The influence of object light intensity and reference light intensity ratio on the reconstructed image quality of hologram is analyzed. The standard resolution plate is the research object.By controlling the filtering capability of filter in the object light and reference light path,we can achieve different light intensity ratio of material reference,and record the reflection hologram of standard resolution plate and reconstruct it. The experimental results show that the object and reference light intensity ratio have a significant impact on the quality of hologram recurrent image when the recording distance is known. Therefore,it's the key to determining an appropriate recording distance and the object and reference light intensity ratio for improving the quality of hologram under the condition that other experimental conditions remain unchanged. Through theoretical analysis,the recording distance of the experiment is determined,and the spectrum chart under the recording distance is analyzed to verify the correctness of the recording distance. Under the same record distance,the contrast experiment of light intensity ratio of different material reference is carried out,and the range of light intensity ratio of material reference which can achieve better reconstruction effect is determined. The experimental result will provide theoretical basis and technical conditions for studying the reflective digital holography based on three-dimensional reconstruction.
引文
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[17]王星,马宏伟,王豆豆,等.数字全息参数优化[J].西安科技大学学报,2017,37(1):127-132.WANG Xing,MA Hong-wei,WANG Dou-dou,et al. Digital holography parameter optimization[J]. Journal of Xi’an University of Science and Technology,2017,37(1):127-132.
[18]宋修法,于梦杰,王华英,等.物光与参考光强度比对数字全息再现像质的影响[J].激光技术,2014,38(6):859-862.SONG Xiu-fa,YU Meng-jie,WANG Hua-ying,et al.Effect of reference intensity ratio to object on reconstructed image quality in digital holography[J]. Laser Technology,2014,38(6):859-862.
[19]张北晨,张之江,于瀛洁.基于数字全息图记录方法的实验研究[J].激光杂志,2007,28(4):47-48.ZHANG Bei-chen,ZHANG Zhi-jiang,YU Ying-jie. Discussion about the method of recording digital hologram[J]. Laser Technology,2007,28(4):47-48.
[20] Batenburg K J,Fortes W,Hajdu L,et al. Bounds on the quality of reconstructed images in binary tomography[J]. Discrete Applied Mathematics,2013,161(15):2236-2251.
[21]丁航,胡翠英,翁嘉文,等.相移式反射数字全息显微成像术研究[J].中国激光,2011,38(12):201-206.DING Hang,HU Cui-ying,WENG Jia-wen,et al. Phaseshifting digital holographic microscopy in reflection configuration[J]. Chinese Journal of Lasers,2011,38(12):201-206.
[22] Shang L,Su P G,Sun Z L. Modified sparse representation based image super-resolution reconstruction[C]//International Conference on Intelligent Computing.Springer International Publishing,2015:491-497.
[23]吴杰.反射式数字全息显微测量技术研究[D].苏州:苏州大学,2016.WU Jie. Study of surface topography of MEMS on reflecting microscopic measurement[D]. Suzhou:Suzhou University,2016.
[2]朱余良,周皓,顾济华.数字全息再现像质的改善[J].苏州大学学报(自然科学版),2010,26(3):51-55.ZHU Yu-liang,ZHOU Hao,GU Ji-hua. Improvement of reconstructed image of digital hologram[J]. Journal of Suzhou University(Natural Science),2010,26(3):51-55.
[3]刘诚,朱健强.数字全息形貌测量的基本特性分析[J].强激光与粒子束,2002,14(3):328-330.LIU Cheng,ZHU Jian-qiang. Basic characters of digital holographic profiling[J]. High Power Laser and Particle Beams,2002,14(3):328-330.
[4]周文静,彭娇,于瀛洁.基于数字全息技术的变形测量[C]//全国精密工程学术研讨会,2005:46-51.ZHOU Wen-jing,PENG Jiao,YU Ying-jie. Deformation measurement based on digital holograph[C]//National Symposium on Precision Engineering,2005:46-51.
[5]吕且妮,葛宝臻.数字全息技术及其在粒子场测试中的研究进展[J].光电子·激光,2002,13(10):1087-1091.LV Qie-ni,GE Bao-zhen. Progress of studies on digital holography and its application in particle field[J]. Journal of Optoelectronics·Laser. 2002,13(10):1087-1091.
[6] Yaroslavsky L. Digital holography and digital image processing[M]. Boston:Springer US,2004.
[7]钟丽云,张以谟,吕晓旭,等.数字全息图再现像的分析计算[J].中国激光,2004,31(5):570-574.ZHONG Li-yun,ZHANG Yi-mo,LV Xiao-xu,et al.Some quantitative analysis about digital holographic reconstructed image[J]. Chinese Journal of Lasers,2004,31(5):570-574.
[8] Malallah R,Li H,Kelly D,et al. A review of hologram storage and self-written waveguides formation in photopolymer media[J]. Polymers,2017,9(8):337.
[9] Kawatsuki N,Hasegawa T,Ono H,et al. Formation of polarization gratings and surface relief gratings in photocrosslinkable polymer liquid crystals by polarization holography[J]. Advanced Materials,2010,15(12):991-994.
[10] Zhou Y,Cattley R T,Cario C L,et al. Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications[J]. Nature Protocols,2014,9(7):1533-1548.
[11]王华英,刘飞飞,宋修法,等.高质量等曲率物参光像面数字全息显微系统[J].物理学报,2013,62(2):268-276.WANG Hua-ying,LIU Fei-fei,SONG Xiu-fa,et al. Highquality digital image-plane micro-holographic system with the same wavefront curvature of reference and object wave[J]. Acta Physica Sinica,2013,62(2):268-276.
[12] Zhang Y,Li J,Yang W,et al. Eliminate the influence of zero-order diffraction for wavefront reconstruction with selectable magnification in digital holographic[J]. Optical&Quantum Electronics,2013,45(10):1077-1086.
[13] Vunckx K,Suetens P,Nuyts J. Effect of overlapping projections on reconstruction image quality in multipinhole SPECT[J]. IEEE Transaction on Medical Imaging,2008,27(7):972-983.
[14]张引科,王存帅,郝劲波.改善数字全息再现像质量的背景强度补偿法[J].激光技术,2013,37(2):204-206.ZHANG Yin-ke,WANG Cun-shuai,HAO Jin-bo. Background intensity compensation method for improving the reconstructed image quality of digital hologram[J]. Laser Technology,2013,37(2):204-206.
[15] Mikhailov V N,Weitzel T K,Latychevskaia T Y. Pulse recording of slanted fringe holograms in dupontphotopolymer[J]. Proceedings of SPIE the International Society for Optical Engineering,1998,3294:132-135.
[16]王华英,刘飞飞,宋修法,等.预放大数字全息显微系统的特性分析[J].强激光与粒子束,2013,25(6):1365-1369.WANG Hua-ying,LIU Fei-fei,SONG Xiu-fa,et al. Characteristic analysis of digital holographic microscopy with pre-magnification[J]. High Power Laser and Particle Beams,2013,25(6):1365-1369.
[17]王星,马宏伟,王豆豆,等.数字全息参数优化[J].西安科技大学学报,2017,37(1):127-132.WANG Xing,MA Hong-wei,WANG Dou-dou,et al. Digital holography parameter optimization[J]. Journal of Xi’an University of Science and Technology,2017,37(1):127-132.
[18]宋修法,于梦杰,王华英,等.物光与参考光强度比对数字全息再现像质的影响[J].激光技术,2014,38(6):859-862.SONG Xiu-fa,YU Meng-jie,WANG Hua-ying,et al.Effect of reference intensity ratio to object on reconstructed image quality in digital holography[J]. Laser Technology,2014,38(6):859-862.
[19]张北晨,张之江,于瀛洁.基于数字全息图记录方法的实验研究[J].激光杂志,2007,28(4):47-48.ZHANG Bei-chen,ZHANG Zhi-jiang,YU Ying-jie. Discussion about the method of recording digital hologram[J]. Laser Technology,2007,28(4):47-48.
[20] Batenburg K J,Fortes W,Hajdu L,et al. Bounds on the quality of reconstructed images in binary tomography[J]. Discrete Applied Mathematics,2013,161(15):2236-2251.
[21]丁航,胡翠英,翁嘉文,等.相移式反射数字全息显微成像术研究[J].中国激光,2011,38(12):201-206.DING Hang,HU Cui-ying,WENG Jia-wen,et al. Phaseshifting digital holographic microscopy in reflection configuration[J]. Chinese Journal of Lasers,2011,38(12):201-206.
[22] Shang L,Su P G,Sun Z L. Modified sparse representation based image super-resolution reconstruction[C]//International Conference on Intelligent Computing.Springer International Publishing,2015:491-497.
[23]吴杰.反射式数字全息显微测量技术研究[D].苏州:苏州大学,2016.WU Jie. Study of surface topography of MEMS on reflecting microscopic measurement[D]. Suzhou:Suzhou University,2016.