药用植物miRNA与次生代谢
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摘要
目前对于中草药活性成分的合成及其调控机制的研讨日益备受瞩目,而近年来对其中药用植物的非编码小分子单链RNA (microRNA, miRNA)方面的研讨也迅速展开,并证实药用植物miRNA在活性成分生物合成方面具备重要调控性能。本文针对近年来植物miRNA的生物合成及作用机制、药用植物miRNA、miRNA与药用植物次生代谢产物生物合成等方面的研究情况进行综述,以期为中药材优质育种提供基础性研讨材料,并为其在临床方面的普遍使用开辟新的研究路径。
Studies on the biosynthesis and regulating mechanism of bioactive components in herbs have attracted more and more attention. In recent years, studies on the non–coding small single–stranded RNA(microRNA, miRNA) of medicinal plants have been carried out rapidly. The miRNAs in medicinal plants has been proved to possess regulatory functions on the biosynthesis of active components. This paper reviewed the studies on biosynthesis and related mechanism of plant miRNA, the biosynthesis of medicinal plant miRNA, miRNA and the secondary metabolites in medicinal plants in recent years, in order to provide basic reasearch data for breeding better Chinese medicinal materials varieties and find new investigation idea for further clinical application of medicinal plants.
Studies on the biosynthesis and regulating mechanism of bioactive components in herbs have attracted more and more attention. In recent years, studies on the non–coding small single–stranded RNA(microRNA, miRNA) of medicinal plants have been carried out rapidly. The miRNAs in medicinal plants has been proved to possess regulatory functions on the biosynthesis of active components. This paper reviewed the studies on biosynthesis and related mechanism of plant miRNA, the biosynthesis of medicinal plant miRNA, miRNA and the secondary metabolites in medicinal plants in recent years, in order to provide basic reasearch data for breeding better Chinese medicinal materials varieties and find new investigation idea for further clinical application of medicinal plants.
引文
Biswas S,Hazra S,Chattopadhyay S(2016).Identification of conserved miRNAs and their putative target genes in Podophyllum hexandrum(Himalayan Mayapple).Plant Gene,6:82
Boke H,Ozhuner E,Turktas M,et al(2015).Regulation of the alkaloid biosynthesis by miRNA in opium poppy.Plant Biotechnol J,13:409-420
Chen C(2013).Cloning and sequence analysis of AGO1 gene in Panax ginseng(dissertation).Linfen,Shanxi:Shanxi Normal University(in Chinese with English abstract)[陈超(2013).人参中与miRNA调控相关的蛋白AG01基因克隆与序列分析(学位论文).山西临汾:山西师范大学]
Chen YL(2017).Identification of microRNA and analysis of target genes in Changbai Mountain ginseng(dissertation).Guangzhou:Guangdong Pharmaceutical University(in Chinese with English abstract)[陈艳琳(2017).长白山人参microRNA鉴定及靶基因分析研究(学位论文).广州:广东药科大学]
Dong M(2013).Detection and identification of target genes of Typhonium giganteum Engl(dissertation).Hangzhou:Zhejiang Sci-Tech University(in Chinese with English abstract)[董淼(2013).半夏miRNA的检测及其靶基因的鉴定(学位论文).杭州:浙江理工大学]
Fan R,Li Y,Li C,et al(2015).Differential microRNA analysis of glandular trichomes and young leaves in Xanthium strumarium L.reveals their putative roles in regulating terpenoid biosynthesis.PLoS One,10:e0139002
Guo XR,Yang XB,Wang HQ,et al(2016).Cloning and expression analysis of miR408 precursor sequences from Salvia miltiorrhiza.Plant Sci J,34:430-438(in Chinese with English abstract)[郭晓荣,杨新兵,王怀琴等(2016).丹参miR408基因前体序列的克隆及表达分析.植物科学学报,34:430-438]
Hazra S,Bhattacharyya D,Chattopadhyay S(2017).Methyl jasmonate regulates podophyllotoxin accumulation in Podophyllum hexandrum by altering the ROS-responsive podophyllotoxin pathway gene expression additionally through the down regulation of few interfering miRNAs.Front Plant Sci,8:164
Jia L(2015).Identification of miRNAs in midbeny and function of midberryderived miRNA in silkworm(dissertation).Chongqing:Southwest University(in Chinese with English abstract)[贾凌(2015).桑树miRNA的鉴定及其在蚕桑互作中的功能研究(学位论文).重庆:西南大学]
Jiang QH(2015).Deep sequencing identifies tissue-specific microRNAs and their target in Salvia miltiorrhiza(dissertation).Hangzhou:Zhejiang Normal University(in Chinese with English abstract)[姜青华(2015).丹参miRNAs组织特异表达谱及其靶基因鉴定(学位论文).杭州:杭州师范大学]
Li YX,Wang N,Wei CX,et al(2012).Bioinformatic prediction of microRNAs in Glycyrrhiza uralensis and their target genes.Chin Tradit Herbal Drugs,47:2506-2514(in Chinese with English abstract)[李华云,王宁,韦春香等(2016).甘草microRNA及其靶基因的生物信息学预测.中草药,47:2506-2514]
Liu J,Wang Y,Jiang C,et al(2017).Regulation of fatty acid and flavonoid biosynthesis by miRNAs in Lonicera japonica.RSC Adv,7:35426
Mathiyalagan R,Subramaniyam S,Natarajan S,et al(2013).Insilico profiling of microRNAs in Korean ginsengg(Panax ginseng Meyer).J Ginseng Res,37:227-247
Pani A,Mahapatra RK(2013).Computational identification of microRNAs and their targets in Catharanthus roseus expressed sequence tags.Genomics Data,1:2-6
Prakash P,Ghosliya D,Gupta V(2015).Identification of conserved and novel microRNAs in Catharanthus roseus by deep sequencing and computational prediction of their potential targets.Gene,554:181-195
Sharma D,Tiwari M,Pandey A,et al(2016).MicroRNA858is apotential regulator of phenylpropanoid pathway and plant development.Plant Physiol,171:944
Shen EM,Singh SK,Ghosh JS,et al(2017).The miRNA of Catharanthus roseus:identification,expression analysis,and potential roles of microRNAs in regulation of terpenoid indole alkaloid biosynthesis.Sci Rep,7:43027
Singh N,Sharma A(2014).In-silico identification of miRNAs and their regulating target functions in Ocimum basilicum.Gene,552:277
Singh N,Srivatava S,Shasany AK,et al(2016).Identification of miRNAs and their targets involved in the secondary metabolic pathways of Mentha spp.Comput Biol Chem,64:154
Wang L,Zhao JG,Luo K,et al(2016).Deep sequencing discovery and profiling of conserved and novel miRNAs in the ovule of Ginkgo biloba.Trees,30:1557-1167
Wei CX,Xiao ZH,Huang Y,et al(2012).Prediction of microRNAs and drought expression pattern analysis in Astragalus membranaceus.Chin Tradit Herbal Drugs,48:155-164(in Chinese with English abstract)[韦春香,肖自华,黄曦等(2017).黄芪miRNA的预测及其干旱表达模式分析.中草药,48:155-164]
Wu B,Li Y,Yan HX,et al(2012).Comprehensive transcriptome analysis reveals novel genes involved in cardiac glycoside biosynthesis and mlncRNAs associated with secondary metabolism and stress response in Digitalis purpurea.BMC Genomics,13:15
Xie M,Zhang S,Yu B,et al(2015).MicroRNA biogenesis,degradation and activity in plants.Cell Mol Sci,72:87-99
Xu S,Jiang YM,Wang N,et al(2016).Identification and differential regulation of microRNAs in response to methyl jasmonate treatment in Lycoris aurea by deep sequencing.BMC Genomics,17:789
Yang K,Lan M,Xing SF,et al(2018).The application of plant miRNA in the research of material base of traditional Chinese medicine.Chin J Chin Mater Med,27:4-6(in Chinese with English abstract)[杨康,蔺曼,刑韶芳等(2018).植物mRNA在中药物质基础研究中的应用.中国民族民间医药,27:4-6]
Yang XB(2017).Cloning and functional analysis of miR156precursor sequence of Salvia miltiorrhiza Bge(dissertation).Linfen,Shanxi:Shanxi Normal University(in Chinese with English abstract)[杨新兵(2017).丹参mi R156前体序列的克隆及功能研究(学位论文).山西临汾:山西师范大学]
Yang ZL(2015).Identification and different expression of Dendrobium officinale mi RNAs(dissertation).Hangzhou:Zhejiang Sci-Tech University(in Chinese with English abstract)[杨支力(2015).铁皮石斛miRNA的检测及差异表达研究(学位论文).杭州:浙江理工大学]
Zhang M,Dong YS,Nie L,et al(2015).High-throughput sequencing reveals miRNA effects on the primary and secondary production properties in long-term subcultured Taxus cells.Front Plant Sci,6:604
Zhang Q(2015).Identification and expression analysis of microRNAs in Ginkgo biloba var.epiphylla Mak(dissertation).Taian,Shandong:Shandong Agricultural University(in Chinese with English abstract)[张倩(2015).叶籽银杏miRNA鉴定与表达分析(学位论文).山东泰安:山东农业大学]
Zhang T(2017).Source identification,quality evaluation and miRNA analysis of milk thistle[Silybum mariauum(L.)Gaertn](dissertation).Hangzhou:Zhejiang Sci-Tech University(in Chinese with English abstract)[张通(2017).水飞蓟的基源鉴定与质量评价及其miRNA的分析(学位论文).杭州:浙江理工大学]
Zhao CL,Li XE,Du XW,et al(2014).Bioinformatic prediction and validation of conserved microRNAs and their target genes in Rehmannia glutinosa.Chin Tradit Herbal Drugs,45:1129-1135(in Chinese with English abstract)[赵春丽,李先恩,都晓伟等(2014).地黄microR-NAs和靶基因的生物信息学预测及验证.中草药,45:1129-1135]
Zhao LD(2015).Studies on the expression of miR5753 and miR5251 and their regulation of flavonoids biosynthesis in Safflower(dissertation).Changchun:Jilin Agricultural University(in Chinese with English abstract)[赵利旦(2015).红花miR5753和miR5251基因表达分析及其对黄酮合成调控的研究(学位论文).长春:吉林农业大学]
Zeng QF,Zeng GY,Zhou YJ(2015).MicroRNA in medicinal plants.Chin J Biochem Mol Biol,31:346-351(in Chinese with English abstract)[曾奇峰,曾光尧,周应军(2015).MicroRNA与药用植物.中国生物化学与分子生物学报,346-351]
Zeng S,Liu Y,Pan L,et al(2015).Identification and characterization of miRNAs in ripening fruit of Lycium barbarum L.using highthroughput sequencing.Front Plant Sci,6:778
Zhen Z,Li XH,Liu JX,et al(2015).Honeysuckle-encoded atypical micro RNA2911 directly targets influenza A viruses.Cell Res,25:39-49
Zhou FM,Bai ZC,Lu SF(2013).MicroRNA in medicinal plants.Chin Tradit Herbal Drugs,44:232-237(in Chinese with English abstract)[周芳名,白志川,卢善发(2013).药用植物microRNA.中草药,44:232-237]
Zhu FJ,Liu J,Yu NJ,et al(2017).Progress on miRNA-based secondary metabolism regulation in medicinal plants.Chem Life,37:1056-1064(in Chinese with English abstract)[朱凤洁,刘娟,俞年军等(2017).药用植物miRNA调控次生代谢的研究进展.生命的化学,37:1056-1064]
Boke H,Ozhuner E,Turktas M,et al(2015).Regulation of the alkaloid biosynthesis by miRNA in opium poppy.Plant Biotechnol J,13:409-420
Chen C(2013).Cloning and sequence analysis of AGO1 gene in Panax ginseng(dissertation).Linfen,Shanxi:Shanxi Normal University(in Chinese with English abstract)[陈超(2013).人参中与miRNA调控相关的蛋白AG01基因克隆与序列分析(学位论文).山西临汾:山西师范大学]
Chen YL(2017).Identification of microRNA and analysis of target genes in Changbai Mountain ginseng(dissertation).Guangzhou:Guangdong Pharmaceutical University(in Chinese with English abstract)[陈艳琳(2017).长白山人参microRNA鉴定及靶基因分析研究(学位论文).广州:广东药科大学]
Dong M(2013).Detection and identification of target genes of Typhonium giganteum Engl(dissertation).Hangzhou:Zhejiang Sci-Tech University(in Chinese with English abstract)[董淼(2013).半夏miRNA的检测及其靶基因的鉴定(学位论文).杭州:浙江理工大学]
Fan R,Li Y,Li C,et al(2015).Differential microRNA analysis of glandular trichomes and young leaves in Xanthium strumarium L.reveals their putative roles in regulating terpenoid biosynthesis.PLoS One,10:e0139002
Guo XR,Yang XB,Wang HQ,et al(2016).Cloning and expression analysis of miR408 precursor sequences from Salvia miltiorrhiza.Plant Sci J,34:430-438(in Chinese with English abstract)[郭晓荣,杨新兵,王怀琴等(2016).丹参miR408基因前体序列的克隆及表达分析.植物科学学报,34:430-438]
Hazra S,Bhattacharyya D,Chattopadhyay S(2017).Methyl jasmonate regulates podophyllotoxin accumulation in Podophyllum hexandrum by altering the ROS-responsive podophyllotoxin pathway gene expression additionally through the down regulation of few interfering miRNAs.Front Plant Sci,8:164
Jia L(2015).Identification of miRNAs in midbeny and function of midberryderived miRNA in silkworm(dissertation).Chongqing:Southwest University(in Chinese with English abstract)[贾凌(2015).桑树miRNA的鉴定及其在蚕桑互作中的功能研究(学位论文).重庆:西南大学]
Jiang QH(2015).Deep sequencing identifies tissue-specific microRNAs and their target in Salvia miltiorrhiza(dissertation).Hangzhou:Zhejiang Normal University(in Chinese with English abstract)[姜青华(2015).丹参miRNAs组织特异表达谱及其靶基因鉴定(学位论文).杭州:杭州师范大学]
Li YX,Wang N,Wei CX,et al(2012).Bioinformatic prediction of microRNAs in Glycyrrhiza uralensis and their target genes.Chin Tradit Herbal Drugs,47:2506-2514(in Chinese with English abstract)[李华云,王宁,韦春香等(2016).甘草microRNA及其靶基因的生物信息学预测.中草药,47:2506-2514]
Liu J,Wang Y,Jiang C,et al(2017).Regulation of fatty acid and flavonoid biosynthesis by miRNAs in Lonicera japonica.RSC Adv,7:35426
Mathiyalagan R,Subramaniyam S,Natarajan S,et al(2013).Insilico profiling of microRNAs in Korean ginsengg(Panax ginseng Meyer).J Ginseng Res,37:227-247
Pani A,Mahapatra RK(2013).Computational identification of microRNAs and their targets in Catharanthus roseus expressed sequence tags.Genomics Data,1:2-6
Prakash P,Ghosliya D,Gupta V(2015).Identification of conserved and novel microRNAs in Catharanthus roseus by deep sequencing and computational prediction of their potential targets.Gene,554:181-195
Sharma D,Tiwari M,Pandey A,et al(2016).MicroRNA858is apotential regulator of phenylpropanoid pathway and plant development.Plant Physiol,171:944
Shen EM,Singh SK,Ghosh JS,et al(2017).The miRNA of Catharanthus roseus:identification,expression analysis,and potential roles of microRNAs in regulation of terpenoid indole alkaloid biosynthesis.Sci Rep,7:43027
Singh N,Sharma A(2014).In-silico identification of miRNAs and their regulating target functions in Ocimum basilicum.Gene,552:277
Singh N,Srivatava S,Shasany AK,et al(2016).Identification of miRNAs and their targets involved in the secondary metabolic pathways of Mentha spp.Comput Biol Chem,64:154
Wang L,Zhao JG,Luo K,et al(2016).Deep sequencing discovery and profiling of conserved and novel miRNAs in the ovule of Ginkgo biloba.Trees,30:1557-1167
Wei CX,Xiao ZH,Huang Y,et al(2012).Prediction of microRNAs and drought expression pattern analysis in Astragalus membranaceus.Chin Tradit Herbal Drugs,48:155-164(in Chinese with English abstract)[韦春香,肖自华,黄曦等(2017).黄芪miRNA的预测及其干旱表达模式分析.中草药,48:155-164]
Wu B,Li Y,Yan HX,et al(2012).Comprehensive transcriptome analysis reveals novel genes involved in cardiac glycoside biosynthesis and mlncRNAs associated with secondary metabolism and stress response in Digitalis purpurea.BMC Genomics,13:15
Xie M,Zhang S,Yu B,et al(2015).MicroRNA biogenesis,degradation and activity in plants.Cell Mol Sci,72:87-99
Xu S,Jiang YM,Wang N,et al(2016).Identification and differential regulation of microRNAs in response to methyl jasmonate treatment in Lycoris aurea by deep sequencing.BMC Genomics,17:789
Yang K,Lan M,Xing SF,et al(2018).The application of plant miRNA in the research of material base of traditional Chinese medicine.Chin J Chin Mater Med,27:4-6(in Chinese with English abstract)[杨康,蔺曼,刑韶芳等(2018).植物mRNA在中药物质基础研究中的应用.中国民族民间医药,27:4-6]
Yang XB(2017).Cloning and functional analysis of miR156precursor sequence of Salvia miltiorrhiza Bge(dissertation).Linfen,Shanxi:Shanxi Normal University(in Chinese with English abstract)[杨新兵(2017).丹参mi R156前体序列的克隆及功能研究(学位论文).山西临汾:山西师范大学]
Yang ZL(2015).Identification and different expression of Dendrobium officinale mi RNAs(dissertation).Hangzhou:Zhejiang Sci-Tech University(in Chinese with English abstract)[杨支力(2015).铁皮石斛miRNA的检测及差异表达研究(学位论文).杭州:浙江理工大学]
Zhang M,Dong YS,Nie L,et al(2015).High-throughput sequencing reveals miRNA effects on the primary and secondary production properties in long-term subcultured Taxus cells.Front Plant Sci,6:604
Zhang Q(2015).Identification and expression analysis of microRNAs in Ginkgo biloba var.epiphylla Mak(dissertation).Taian,Shandong:Shandong Agricultural University(in Chinese with English abstract)[张倩(2015).叶籽银杏miRNA鉴定与表达分析(学位论文).山东泰安:山东农业大学]
Zhang T(2017).Source identification,quality evaluation and miRNA analysis of milk thistle[Silybum mariauum(L.)Gaertn](dissertation).Hangzhou:Zhejiang Sci-Tech University(in Chinese with English abstract)[张通(2017).水飞蓟的基源鉴定与质量评价及其miRNA的分析(学位论文).杭州:浙江理工大学]
Zhao CL,Li XE,Du XW,et al(2014).Bioinformatic prediction and validation of conserved microRNAs and their target genes in Rehmannia glutinosa.Chin Tradit Herbal Drugs,45:1129-1135(in Chinese with English abstract)[赵春丽,李先恩,都晓伟等(2014).地黄microR-NAs和靶基因的生物信息学预测及验证.中草药,45:1129-1135]
Zhao LD(2015).Studies on the expression of miR5753 and miR5251 and their regulation of flavonoids biosynthesis in Safflower(dissertation).Changchun:Jilin Agricultural University(in Chinese with English abstract)[赵利旦(2015).红花miR5753和miR5251基因表达分析及其对黄酮合成调控的研究(学位论文).长春:吉林农业大学]
Zeng QF,Zeng GY,Zhou YJ(2015).MicroRNA in medicinal plants.Chin J Biochem Mol Biol,31:346-351(in Chinese with English abstract)[曾奇峰,曾光尧,周应军(2015).MicroRNA与药用植物.中国生物化学与分子生物学报,346-351]
Zeng S,Liu Y,Pan L,et al(2015).Identification and characterization of miRNAs in ripening fruit of Lycium barbarum L.using highthroughput sequencing.Front Plant Sci,6:778
Zhen Z,Li XH,Liu JX,et al(2015).Honeysuckle-encoded atypical micro RNA2911 directly targets influenza A viruses.Cell Res,25:39-49
Zhou FM,Bai ZC,Lu SF(2013).MicroRNA in medicinal plants.Chin Tradit Herbal Drugs,44:232-237(in Chinese with English abstract)[周芳名,白志川,卢善发(2013).药用植物microRNA.中草药,44:232-237]
Zhu FJ,Liu J,Yu NJ,et al(2017).Progress on miRNA-based secondary metabolism regulation in medicinal plants.Chem Life,37:1056-1064(in Chinese with English abstract)[朱凤洁,刘娟,俞年军等(2017).药用植物miRNA调控次生代谢的研究进展.生命的化学,37:1056-1064]