星点设计-响应面法优化闹羊花中二萜的提取工艺
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摘要
目的:为了得到超声波提取闹羊花中二萜物质的最佳提取工艺。方法:通过单因素试验考察了料液比、提取温度和提取时间对二萜提取量的影响规律,并采用星点设计-响应面法对其进行了优化。结果:料液比为1∶40. 29,提取温度为63. 15℃,提取时间为40. 53 min时,预测最大的闹羊花二萜提取量为65. 27 mg·g-1。考虑实际情况对工艺条件微调,验证了闹羊花中二萜提取量的实验值与预测相对误差较小。结论:优化的工艺条件可为扩大实际生产提供一定的技术支持和参考价值。
Objective: To obtain the optimum extraction process of diterpene from Rhododendron molle via the ultrasonic method. Methods: The effect of the ratio of material to liquid,the extraction temperature and the extraction time on the extraction of diterpene was studied by single factor experiment. The optimum extraction conditions were obtained by the central composite design and response surface methodology. Results: The optimized extraction condition were as follows: ratio of material to liquid of 1∶40. 29,extraction temperature of 63. 15 and extraction time of 40. 53 min. Under the optimized reaction conditions,the content of diterpene was 65. 27 mg·g-1. The experimental value of the diterpene extraction in Rh. molle was smaller than that of the prediction. Conclusion: The optimized extraction conditions can provide some technical support and reference value for the expansion of actual production.
Objective: To obtain the optimum extraction process of diterpene from Rhododendron molle via the ultrasonic method. Methods: The effect of the ratio of material to liquid,the extraction temperature and the extraction time on the extraction of diterpene was studied by single factor experiment. The optimum extraction conditions were obtained by the central composite design and response surface methodology. Results: The optimized extraction condition were as follows: ratio of material to liquid of 1∶40. 29,extraction temperature of 63. 15 and extraction time of 40. 53 min. Under the optimized reaction conditions,the content of diterpene was 65. 27 mg·g-1. The experimental value of the diterpene extraction in Rh. molle was smaller than that of the prediction. Conclusion: The optimized extraction conditions can provide some technical support and reference value for the expansion of actual production.
引文
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[14]成英,何兴金.续随子种子中两种大环二萜类物质的提取工艺[J].时珍国医国药,2011,22(5):1267-1269.
[2]张枝润.两种杜鹃花属植物化学成分及质量标准研究[D].昆明:昆明理工大学,2012.
[3]陈刚,金慧子,李雪峰,等.杜鹃花属植物的化学成分及药理研究进展[J].药学实践杂志,2008,26(4):255-257.
[4]海广范,张慧,郭兰青.二萜类化合物药理学作用研究进展[J].新乡医学院学报,2015,32(1):77-80.
[5]王新玲,王晓梅,胡君萍,等.新疆丹参总二萜醌类化合物提取工艺研究[J].时珍国医国药,2013,24(3):652-653.
[6]邓韵,蔡妙颜,田野,等.溪黄草不同溶剂提取物中总二萜含量的测定[J].现代食品科技,2005,21(2):153-154.
[7]李婷,侯晓东,陈文学,等.超声波萃取技术的研究现状及展望[J].安徽农业科学,2006,34(13):3188-3190.
[8] Romdhane M,Gourdon C. Investigation in solid-liquid extraction:influence of ultrasound[J]. Chem Eng J,2002,87(1):11-19.
[9] Li H,Pordesimo L,Weiss J. High intensity ultrasound-assisted extraction of oil from soybeans[J]. Food Res Int,2004,37(7):731-738.
[10]吴伟,崔光华.星点设计-效应面优化法及其在药学中的应用[J].国际药学研究杂志,2000,27(5):292-298.
[11]段志芳,陈庆华.超声波法提取溪黄草中总二萜的最佳工艺研究[J].时珍国医国药,2006,17(12):2436-2438.
[12]韩瑞亭,万国盛,孙博航,等.苦石莲中总二萜的含量测定[J].中国实验方剂学杂志,2013,19(6):84-86.
[13]孙庆磊,梁月荣,陆建良.超声波在茶叶提取中的应用[J].茶叶,2006,32(2):79-82.
[14]成英,何兴金.续随子种子中两种大环二萜类物质的提取工艺[J].时珍国医国药,2011,22(5):1267-1269.