拟南芥bzip19-2/bzip23-1/bzip24-2三突变体植株获取及其PCR鉴定
|
摘要
近年来植物所受非生物胁迫的影响日益严峻,是造成农业减产的主要因素之一。bZIP类转录因子参与多种生物学功能并起着重要调控作用,尤其是对植物生长发育以及逆境胁迫应答的调控。因此文章以拟南芥bZIP19、bZIP23、bZIP24基因的突变体为实验材料,通过遗传杂交技术构建bzip19-2/bzip23-1/bzip24-2三突变体,结合聚合酶链式反应(polymerase chain reaction,PCR)鉴定技术,最终获得纯合三突变体实验材料,为研究bZIP类转录因子的功能及其间相互作用奠定了基础。
In recent years,the impact of abiotic stress on plants has become increasingly serious,which is one of the main factors contributing to the reduction of agricultural production.The bZIP transcription factors(TFs)are involved in a variety of biological functions and play an important regulatory role,especially for the regulation of plant growth and development as well as abiotic stress response.In this paper,bzip19-2/bzip23-1/bzip24-2 triple-mutant experimental material was constructed by genetic cross with mutants of bZIP19,bZIP23 and bZIP24 in Arabidopsis thaliana,and the polymerase chain reaction(PCR)identification technology was used to obtain homozygous triple-mutant.The study can be valuable for exploring the function of bZIP TFs and their interaction.
In recent years,the impact of abiotic stress on plants has become increasingly serious,which is one of the main factors contributing to the reduction of agricultural production.The bZIP transcription factors(TFs)are involved in a variety of biological functions and play an important regulatory role,especially for the regulation of plant growth and development as well as abiotic stress response.In this paper,bzip19-2/bzip23-1/bzip24-2 triple-mutant experimental material was constructed by genetic cross with mutants of bZIP19,bZIP23 and bZIP24 in Arabidopsis thaliana,and the polymerase chain reaction(PCR)identification technology was used to obtain homozygous triple-mutant.The study can be valuable for exploring the function of bZIP TFs and their interaction.
引文
[1] SCHWECHHEIMER C,BEVAN M.The regulation of transcription factor activity in plants[J].Trends in Plant Science,1998,3(10):378-383.
[2] LIU L,WHITE M J,MACRAE T H.Transcription factors and their genes in higher plants[J].European Journal of Biochemistry,1999,262(2):247-257.
[3] EULGEM T,RUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science,2000,5(5):199-206.
[4] JAKOBY M,WEISSHAAR B,DR¨OGE-LASER W,et al.bZIP transcription factors in Arabidopsis[J].Trends in Plant Science,2002,7(3):106-111.
[5] SORNARAJ P,LUANG S,LOPATO S,et al.Basic leucine zipper(bZIP)transcription factors involved in abiotic stresses:a molecular model of a wheat bZIP factor and implications of its structure in function[J].Biochimica et Biophysica Acta(BBA)-General Subjects,2016,1860(1):46-56.
[6] FOSTER R,IZAWA T,CHUA N H.Plant bZIP proteins gather at ACGT elements[J].Faseb Journal,1994,8(2):192-200.
[7] ASSUNCAO A G,HERRERO E,LIN Y F,et al.Arabidopsis thaliana transcription factors bZIP19and bZIP23regulate the adaptation to zinc deficiency[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(22):10296-10301.
[8] FUKAO T,XIONG L.Genetic mechanisms conferring adaptation to submergence and drought in rice:simple or complex[J].Current Opinion in Plant Biology,2013,16(2):196-204.
[9] YANG O S,POPOVA O V,SUTHOFF U,et al.The Arabidopsis basic leucine zipper transcription factor AtbZIP24regulates complex transcriptional networks involved in abiotic stress resistance[J].Gene,2009,436(1/2):45-55.
[10] LAPERCHE V,LOGAN T J,PRANITHA GADDAM A,et al.Effect of apatite amendments on plant uptake of lead from contaminated soil[J].Environmental Science&Technology,2013,31(10):2745-2753.
[2] LIU L,WHITE M J,MACRAE T H.Transcription factors and their genes in higher plants[J].European Journal of Biochemistry,1999,262(2):247-257.
[3] EULGEM T,RUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science,2000,5(5):199-206.
[4] JAKOBY M,WEISSHAAR B,DR¨OGE-LASER W,et al.bZIP transcription factors in Arabidopsis[J].Trends in Plant Science,2002,7(3):106-111.
[5] SORNARAJ P,LUANG S,LOPATO S,et al.Basic leucine zipper(bZIP)transcription factors involved in abiotic stresses:a molecular model of a wheat bZIP factor and implications of its structure in function[J].Biochimica et Biophysica Acta(BBA)-General Subjects,2016,1860(1):46-56.
[6] FOSTER R,IZAWA T,CHUA N H.Plant bZIP proteins gather at ACGT elements[J].Faseb Journal,1994,8(2):192-200.
[7] ASSUNCAO A G,HERRERO E,LIN Y F,et al.Arabidopsis thaliana transcription factors bZIP19and bZIP23regulate the adaptation to zinc deficiency[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(22):10296-10301.
[8] FUKAO T,XIONG L.Genetic mechanisms conferring adaptation to submergence and drought in rice:simple or complex[J].Current Opinion in Plant Biology,2013,16(2):196-204.
[9] YANG O S,POPOVA O V,SUTHOFF U,et al.The Arabidopsis basic leucine zipper transcription factor AtbZIP24regulates complex transcriptional networks involved in abiotic stress resistance[J].Gene,2009,436(1/2):45-55.
[10] LAPERCHE V,LOGAN T J,PRANITHA GADDAM A,et al.Effect of apatite amendments on plant uptake of lead from contaminated soil[J].Environmental Science&Technology,2013,31(10):2745-2753.