红花油体体外稳定性的研究
|
摘要
油体属于储藏脂肪的亚细胞单位,它的表面覆盖一层磷脂和油体蛋白,具有非常稳定的结构。文章以红花油体为研究对象,从PBS、NaOH、ddH2O、Tricine和Tris-HCl中筛选出最适合红花油体的提取溶剂并且制备了红花油体悬浮液,考察了不同条件下的红花油体悬浮液体外稳定性。结果表明:通过分析红花油体提取率、粒径和电位,确定PBS为红花油体最佳提取溶剂,在提取的过程中对不同料液比进行比较,确定红花油体提取的最佳料液比为1∶10。同时,考察了pH、盐离子浓度、表面活性剂、处理温度对油体体外稳定性的影响,确定了油体在体外稳定存在的pH为8,盐离子浓度为100 mmol/L,咪唑啉阳离子表面活性剂对红花油体悬浮液稳定效果较好,在合适的pH、盐离子浓度和表面活性剂存在的条件下红花油体悬浮液可以在一定时间内保持稳定存在。
The oil body is subcellular organelle for storing fat. Its surface is covered with a layer of phospholipids and oleosins, and its structure is stable. The oil body from Carthamus tinctorious.L was taken as experimental material, and the optimal extraction solvent was selected from PBS, NaOH, ddH_2O, Tricine and Tris-HCl. The oil body suspension liquid was prepared. The stability of oil body in vitro was investigated in different conditions. Results showed that PBS was determined to be the best extraction solvent by the analysis of extraction ratio, particle size and electric potential. The optimum solid-liquid ratio was determined to be 1∶10 by the comparison of different solid-liquid ratios during extraction process. The effects of pH value, salt ion concentration, surfactant and treatment temperature on the stability of the oil body in vitro were investigated. The pH value was 8. The salt ion concentration was 100 mmol/L. The tetrahydroglyoxaline cationic surfactant had a better stabilizing effect on the oil body suspension of Carthamus tinctorious.L. The oil body suspension of Carthamus tinctorious.L can remain stable for a certain period of time with a suitable pH, salt ion concentration and presence of a surfactant.
The oil body is subcellular organelle for storing fat. Its surface is covered with a layer of phospholipids and oleosins, and its structure is stable. The oil body from Carthamus tinctorious.L was taken as experimental material, and the optimal extraction solvent was selected from PBS, NaOH, ddH_2O, Tricine and Tris-HCl. The oil body suspension liquid was prepared. The stability of oil body in vitro was investigated in different conditions. Results showed that PBS was determined to be the best extraction solvent by the analysis of extraction ratio, particle size and electric potential. The optimum solid-liquid ratio was determined to be 1∶10 by the comparison of different solid-liquid ratios during extraction process. The effects of pH value, salt ion concentration, surfactant and treatment temperature on the stability of the oil body in vitro were investigated. The pH value was 8. The salt ion concentration was 100 mmol/L. The tetrahydroglyoxaline cationic surfactant had a better stabilizing effect on the oil body suspension of Carthamus tinctorious.L. The oil body suspension of Carthamus tinctorious.L can remain stable for a certain period of time with a suitable pH, salt ion concentration and presence of a surfactant.
引文
[1] Huang A H C. Oil bodies and oleosins in seeds[J]. Plant Mol Biol, 1992, 43: 177-200.
[2] Tzen J T C, Cao Y Z, Lauren P, et al. Lipids and structure of seed oil bodies from diverse species[J]. Plant Physiol, 1993, 101:267-276.
[3] Tzen J T C, Huang A H C. Surface structure and properties of plant seed oil bodies[J]. J Cell Biol, 1992, 117:327-335.
[4] Slack C R,Bcrtaus W S, Shaw B P, et al. Some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower and linseed[J]. Biochem J, 1980, 190:551-561.
[5] Iwanaga D, Gray D A, Fisk I D, et al. Extraction and characterization of oil bodies from soy beans: A natural source of pre-emulsified soybean oil [J]. Journal of Agricultural and Food Chemistry,2007, 55: 8711-8716.
[6] Lacey D J,Beaudin F, Dempsey C E, et al. The accumulation of triacylglycerols within the endoplasmatic reticulum of develoing seeds of Helianthus annuus[J]. Plant J, 1999, 17:393-405.
[7] 官丽莉,董程,李海龙,等.红花油体冻干粉的制备及稳定性分析[J]. 吉林农业大学学报,2018,40(5):640-646.
[8] Tai S S K, Chen M C M, Peng C C, et al.Gene family of oleosin isoforms in sesame seed oil bodies and their structural stabilization to reconstituted oil bodies[J]. Biosci Biotechnol Biochem, 2002, 66:2146-2153.
[9] Roberts N, Scott R,Tzen J. Recent biotechnological applications using oleosins[J]. Open Biotech J, 2008, 2:13-21.
[10] Chiang C J, Chen H C,Kuo H F, et al. A simple and effective method to prepare immobilized enzymes using artificial oil bodies[J]. Enzyme Microb Tech, 2006, 39:1152-1158.
[11] Chiang C J, Chen H C, Chao Y P,et al. One-step purification of insoluble hydantoinase overproduced in Escherichia coli[J]. Protein Expr Purif, 2007, 52:14-18.
[12] Bhatla S C, Vandana S, Kaushik V. Recent developments in the localization of oil bodyassociated signalling molecules during lipolysis in oilseeds[J]. Plant Signal Behav, 2009, 4:176-182.
[13] Blixt O, Allin K, Pereira L, et al. Efficient chemoenzymatic synthesis of O-linked sialyl oligosaccharides[J]. J Am Chem Soc, 2002, 124: 5739-5746.
[14] Nikiforidis C V, Karkani O A, Kiosseoglou V. Exploitation of maize germ for the preparation of a stable oil body nanoemulsion using a combined aqueous extraction ultrafiltration method[J]. Food Hydrocolloids, 2011,25:1122-1127.
[15] Nikiforidis C V, Kiosseoglou V. Physicochemical stability of maize germ oil body emulsions as influenced by oil body surface xanthangum interactions[J]. Journal of Agricultural and Food Chemistry, 2010, 58:527-532.
[16] Tzen J T C, Lai Y K, Chan K L, et al. Oleosion isoforms of high and low molecular weights are present in the oil bodies of diverse seed species[J]. Plant Physiol,1990,94:1282-1289.
[17] 曹艳云,陈业明,华欲飞,等.高碱性提取的大豆油体的成分变化研究[J]. 中国粮油学报,2014,29(3):38-41.
[18] Jacks T J, Hensarling T P, Neucere J N, et al.Isolation and physicochemical characterization of half-unit membranes of oil seed lipid bodies [J].Journal of the American Oil Chemists Society,1990,67:353-361.
[19] Vesna K, Ganesh K A, Martin H, et al. Protein and lipid composition analysis of oil bodies from two Brassica napus cultivars[J]. Proteomics, 2006, 6: 4586-4598.
[20] Gu Y S, Decker E A, McClements D J. Application of multi-component biopolym erlayers to improve the freeze-thaw stability of oil-in-water emulsions: β-lactoglob ulin-ι-carrageenan-gelatin[J]. Journal of Food Engineering, 2007, 80:1246-1254.
[21] Nikiforidis C V, Karkani O A, Kiosseoglou V. Exploitation of maize germ for the preparation of a stable oilbody nanoemulsion using a combined aqueous extraction ultrafiltration method[J]. Food Hydrocolloids, 2011, 25:1122-1127.
[2] Tzen J T C, Cao Y Z, Lauren P, et al. Lipids and structure of seed oil bodies from diverse species[J]. Plant Physiol, 1993, 101:267-276.
[3] Tzen J T C, Huang A H C. Surface structure and properties of plant seed oil bodies[J]. J Cell Biol, 1992, 117:327-335.
[4] Slack C R,Bcrtaus W S, Shaw B P, et al. Some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower and linseed[J]. Biochem J, 1980, 190:551-561.
[5] Iwanaga D, Gray D A, Fisk I D, et al. Extraction and characterization of oil bodies from soy beans: A natural source of pre-emulsified soybean oil [J]. Journal of Agricultural and Food Chemistry,2007, 55: 8711-8716.
[6] Lacey D J,Beaudin F, Dempsey C E, et al. The accumulation of triacylglycerols within the endoplasmatic reticulum of develoing seeds of Helianthus annuus[J]. Plant J, 1999, 17:393-405.
[7] 官丽莉,董程,李海龙,等.红花油体冻干粉的制备及稳定性分析[J]. 吉林农业大学学报,2018,40(5):640-646.
[8] Tai S S K, Chen M C M, Peng C C, et al.Gene family of oleosin isoforms in sesame seed oil bodies and their structural stabilization to reconstituted oil bodies[J]. Biosci Biotechnol Biochem, 2002, 66:2146-2153.
[9] Roberts N, Scott R,Tzen J. Recent biotechnological applications using oleosins[J]. Open Biotech J, 2008, 2:13-21.
[10] Chiang C J, Chen H C,Kuo H F, et al. A simple and effective method to prepare immobilized enzymes using artificial oil bodies[J]. Enzyme Microb Tech, 2006, 39:1152-1158.
[11] Chiang C J, Chen H C, Chao Y P,et al. One-step purification of insoluble hydantoinase overproduced in Escherichia coli[J]. Protein Expr Purif, 2007, 52:14-18.
[12] Bhatla S C, Vandana S, Kaushik V. Recent developments in the localization of oil bodyassociated signalling molecules during lipolysis in oilseeds[J]. Plant Signal Behav, 2009, 4:176-182.
[13] Blixt O, Allin K, Pereira L, et al. Efficient chemoenzymatic synthesis of O-linked sialyl oligosaccharides[J]. J Am Chem Soc, 2002, 124: 5739-5746.
[14] Nikiforidis C V, Karkani O A, Kiosseoglou V. Exploitation of maize germ for the preparation of a stable oil body nanoemulsion using a combined aqueous extraction ultrafiltration method[J]. Food Hydrocolloids, 2011,25:1122-1127.
[15] Nikiforidis C V, Kiosseoglou V. Physicochemical stability of maize germ oil body emulsions as influenced by oil body surface xanthangum interactions[J]. Journal of Agricultural and Food Chemistry, 2010, 58:527-532.
[16] Tzen J T C, Lai Y K, Chan K L, et al. Oleosion isoforms of high and low molecular weights are present in the oil bodies of diverse seed species[J]. Plant Physiol,1990,94:1282-1289.
[17] 曹艳云,陈业明,华欲飞,等.高碱性提取的大豆油体的成分变化研究[J]. 中国粮油学报,2014,29(3):38-41.
[18] Jacks T J, Hensarling T P, Neucere J N, et al.Isolation and physicochemical characterization of half-unit membranes of oil seed lipid bodies [J].Journal of the American Oil Chemists Society,1990,67:353-361.
[19] Vesna K, Ganesh K A, Martin H, et al. Protein and lipid composition analysis of oil bodies from two Brassica napus cultivars[J]. Proteomics, 2006, 6: 4586-4598.
[20] Gu Y S, Decker E A, McClements D J. Application of multi-component biopolym erlayers to improve the freeze-thaw stability of oil-in-water emulsions: β-lactoglob ulin-ι-carrageenan-gelatin[J]. Journal of Food Engineering, 2007, 80:1246-1254.
[21] Nikiforidis C V, Karkani O A, Kiosseoglou V. Exploitation of maize germ for the preparation of a stable oilbody nanoemulsion using a combined aqueous extraction ultrafiltration method[J]. Food Hydrocolloids, 2011, 25:1122-1127.