外源一氧化氮对低温胁迫下茶树幼苗生理特性的影响
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摘要
为探究外源一氧化氮(Nitric oxide,NO)供体硝普钠(Sodium nitroprusside,SNP)对低温胁迫下茶树幼苗的缓解作用,采用人工气候室模拟低温胁迫试验,研究了叶面喷施不同浓度SNP对低温胁迫下两年生碧香早茶苗叶片膜脂过氧化程度、渗透调节物质含量及抗氧化酶活性的影响。结果表明,适宜SNP浓度可降低低温胁迫下茶树叶片相对电导率,抑制丙二醛含量的升高,促进脯氨酸、可溶性蛋白和可溶性糖的大量积累,提高超氧化物歧化酶、过氧化氢酶活性,从而缓解低温胁迫对茶树叶片的伤害。试验结果表明,喷施浓度为200μmol·L~(-1)的SNP效果最佳。
In order to investigate the alleviation effect of exogenous nitric oxide(NO) donor sodium nitroprusside(SNP) on tea rooted cuttings under cold stress, artificial climate chamber was used to simulate cold stress on tea rooted cuttings. The effects of SNP on membrane lipid peroxidation, osmotic adjustment substance and antioxidant activity of biennial ‘Bixiangzao' rooted cuttings under cold stress were studied by foliar spray of SNP. The results show that suitable SNP concentration treatment could reduce the relative conductivity of tea leaves, inhibit the elevated malondialdehyde content, promote the accumulation of proline, soluble protein and sugar, and increase the superoxide dismutase, catalase activities, thereby alleviate the damage of cold stress on tea leaves. In addition, the optimal concentration of SNP was 200 μmol·L~(-1).
In order to investigate the alleviation effect of exogenous nitric oxide(NO) donor sodium nitroprusside(SNP) on tea rooted cuttings under cold stress, artificial climate chamber was used to simulate cold stress on tea rooted cuttings. The effects of SNP on membrane lipid peroxidation, osmotic adjustment substance and antioxidant activity of biennial ‘Bixiangzao' rooted cuttings under cold stress were studied by foliar spray of SNP. The results show that suitable SNP concentration treatment could reduce the relative conductivity of tea leaves, inhibit the elevated malondialdehyde content, promote the accumulation of proline, soluble protein and sugar, and increase the superoxide dismutase, catalase activities, thereby alleviate the damage of cold stress on tea leaves. In addition, the optimal concentration of SNP was 200 μmol·L~(-1).
引文
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[14]郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.
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[22]张钢.国外木本植物抗寒性测定方法综述[J].世界林业研究,2005(5):14-20.
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[25]郭经纬,商桑,穆大伟,等.外源NO对低温胁迫下豇豆幼苗生长和生理特性的影响[J].热带作为学报,2015,36(12):2179-2183.
[26]李新.一氧化氮对枳低温应答和多胺合成的影响[D].武汉:华中农业大学,2012.
[27]刘兴旺.一氧化氮提高黄瓜低温耐受性的分子机制[D].北京:中国农业大学,2013.
[28]王宪叶,沈文滩,徐朗莱.外源一氧化氮对渗透胁迫下小麦幼苗叶片膜脂过氧化的缓解作用[J].植物生理与分子生物学学报,2004,30(2):195-200.
[29]Beligni M V,Fath A,Bethke P C,et al.Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layer[J].Plant Physiology,2002,129:1642-1645.
[2]林郑和,钟秋生,陈常颂.茶树抗性育种研究进展[J].福建茶叶,2015,37(4):2-4.
[3]杨再强,韩冬,王学林,等.寒潮过程中4个茶树品种光合特性和保护酶活性变化及品种间差异[J].生态学报,2016,36(3):629-641.
[4]粟本文.茶树抗寒性研究概况[J].茶叶科学简报,1991(4):27-29.
[5]罗军武,唐和平,黄意欢,等.茶树不同抗寒性品种间保护酶类活性的差异[J].湖南农业大学学报(自然科学版),2001,27(2):94-96.
[6]王新超,杨亚军.茶树抗性育种研究现状[J].茶叶科学,2003,23(2):94-98.
[7]黄建安.茶树保护性酶类与抗寒性的关系[J].茶叶科学,1990,10(1):35-40.
[8]Wendehenne D,Courtois C,Besson A,et al.NO-based signaling in plants[M]//Lamattina L,Polacco J C.Nitric oxide in plant growth,development and stress physiology.Berlin:Springer,2007:35-51.
[9]Tian X,Lei Y.Nitric oxide treatment alleviates drought stress in wheat seedlings[J].Biol Plant,2006,50(4):775-778.
[10]Zheng C,Jiang D,Liu F,et al.Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity[J].Environ Exp Bot,2009,67(1):222-227.
[11]Liu X,Wang L,Liu L,et al.Alleviating effect of exogenous nitric oxide in cucumber seedling against chilling stress[J].African Journal Biotechnology,2011,10(21):4380-4386.
[12]陈银萍,王晓梅,杨宗娟,等.NO对低温胁迫下玉米种子萌发及幼苗生理特性的影响[J].农业环境科学学报,2012,31(6):270-277.
[13]黄意欢.茶学实验技术[M].北京:中国农业出版杜,1995.
[14]郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.
[15]孔祥生,易现峰.植物生理学实验技术[M].北京:中国农业出版社,2008.
[16]李合生.植物生理生化实验原理及技术[M].北京:高等教育出版社,2000.
[17]朱政.茶树抗寒性生理指标的筛选及抗寒性鉴定方法的建立[D].合肥:安徽农业大学,2011.
[18]徐洪雷.一氧化氮对黄瓜盐胁迫及低温胁迫缓解作用的研究[D].哈尔滨:东北农业大学,2006.
[19]马向丽,魏小红,龙瑞军,等.外源一氧化氮提高一年生黑麦草抗冷性机制[J].生态学报,2005,25(6):1270-1274.
[20]樊怀福,杜长霞,朱祝军.外源NO对低温胁迫下黄瓜幼苗生长、叶片膜脂过氧化和光合作用的影响[J].浙江农业学报.2011,23(3):538-542.
[21]肖强,郑海雷.一氧化氮与植物胁迫响应[J].植物生理学通讯,2004,40(3):379-384.
[22]张钢.国外木本植物抗寒性测定方法综述[J].世界林业研究,2005(5):14-20.
[23]Zhao M G,Chen L,Zhang L L,et al.Nitric reductase-dependent nitric oxide production is involved in cold acclimation and freezing tolerance in Arabidopsis[J].Plant Physiology,2009,151(2):755-767.
[24]吴旭红,吕成敏,冯晶昊.外源一氧化氮(NO)对低温胁迫下南瓜幼苗氧化损伤的保护效应[J].草业学报,2016,25(12):161-169.
[25]郭经纬,商桑,穆大伟,等.外源NO对低温胁迫下豇豆幼苗生长和生理特性的影响[J].热带作为学报,2015,36(12):2179-2183.
[26]李新.一氧化氮对枳低温应答和多胺合成的影响[D].武汉:华中农业大学,2012.
[27]刘兴旺.一氧化氮提高黄瓜低温耐受性的分子机制[D].北京:中国农业大学,2013.
[28]王宪叶,沈文滩,徐朗莱.外源一氧化氮对渗透胁迫下小麦幼苗叶片膜脂过氧化的缓解作用[J].植物生理与分子生物学学报,2004,30(2):195-200.
[29]Beligni M V,Fath A,Bethke P C,et al.Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layer[J].Plant Physiology,2002,129:1642-1645.