兖州卷柏黄酮对人食管癌细胞增殖及凋亡的影响
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摘要
为探讨兖州卷柏总黄酮对人食管癌EC9706细胞增殖及凋亡的影响,采用CCK8法测定兖州卷柏总黄酮对人食管癌EC9706细胞体外增殖的影响,Hoechst细胞核荧光染色法观察兖州卷柏总黄酮对人食管癌EC9706细胞凋亡的影响,ELISA法检测兖州卷柏总黄酮对人食管癌EC9706细胞一氧化氮(NO)、一氧化氮合酶(NOS)、环鸟苷酸(cGMP)含量的影响.结果显示:兖州卷柏总黄酮能抑制人食管癌EC9706细胞的增殖和促进细胞凋亡,总黄酮处理72 h后,对人食管癌细胞IC_(50)为189.42μg/mL,细胞凋亡率最高为39.67%±3.39%.兖州卷柏总黄酮使人食管癌细胞NO、NOS含量增加,72 h后黄酮浓度为400μg/mL实验组NO、NOS含量比阴性对照组分别增加23.56%和19.42%.而兖州卷柏总黄酮使人食管癌细胞cGMP含量无明显影响.这表明兖州卷柏总黄酮能抑制人食管癌EC9706细胞的增殖并促进细胞凋亡,其机制可能是通过非cGMP依赖途径抑制人食管癌细胞增殖.
To investigate the effect of total flavonoids from S.involventis(TFSI) on proliferation and apoptosis of esophagus carcinoma cells(EC-9706),the proliferation inhibiting and apoptosis ration of TFSI on esophagus earcinoma cells were determined using CCK8 assay and Hoechst-tagged fluorescein methods,respectively.Furthermore,effect of TFSI on the levels of nitric oxide(NO),nitricoxide synthase(NOS) andcyclic guanosine monophosphate(cGMP) in esophagus earcinoma cells(EC-9706) were assayed by ELISA methods.The results showed that TFSI could inhibit the growth and promote apoptosis of esophagus carcinoma cells.After 72 h,50% growth inhibition(IC_(50)) in EC-9706 cells was at concentrations of 189.42 μg/mL,and the highest apoptosis ratio was 39.67%±3.39%.TFSI could increase the levels of NO and NOS.When treated with 400 μg/L TFSI,the levels of NO and NOS increased 23.56% and 19.42% compared with negative control after 72 h.However,TFSI could not significantly affect the level of cGMP.It is indicated that TFSI could inhibit proliferation of esophagus carcinoma cells,and promote apoptosis ration of esophagus carcinoma cells.TFSI inhibit the proliferation of esophagus carcinoma cells may be through non-cGMP pathway.
To investigate the effect of total flavonoids from S.involventis(TFSI) on proliferation and apoptosis of esophagus carcinoma cells(EC-9706),the proliferation inhibiting and apoptosis ration of TFSI on esophagus earcinoma cells were determined using CCK8 assay and Hoechst-tagged fluorescein methods,respectively.Furthermore,effect of TFSI on the levels of nitric oxide(NO),nitricoxide synthase(NOS) andcyclic guanosine monophosphate(cGMP) in esophagus earcinoma cells(EC-9706) were assayed by ELISA methods.The results showed that TFSI could inhibit the growth and promote apoptosis of esophagus carcinoma cells.After 72 h,50% growth inhibition(IC_(50)) in EC-9706 cells was at concentrations of 189.42 μg/mL,and the highest apoptosis ratio was 39.67%±3.39%.TFSI could increase the levels of NO and NOS.When treated with 400 μg/L TFSI,the levels of NO and NOS increased 23.56% and 19.42% compared with negative control after 72 h.However,TFSI could not significantly affect the level of cGMP.It is indicated that TFSI could inhibit proliferation of esophagus carcinoma cells,and promote apoptosis ration of esophagus carcinoma cells.TFSI inhibit the proliferation of esophagus carcinoma cells may be through non-cGMP pathway.
引文
[1] 刘一杰,薛永常.植物黄酮类化合物的研究进展[J].中国生物工程杂志,2016,36(9):81-86.
[2] 李慧娜,田少君.油料作物中黄酮类化合物的提取方法研究进展[J].中国油脂,2017(8):111-115.
[3] 林美珍,许振强.兖州卷柏识别及对肝炎的治疗作用[J].海峡药学,2004,16(5):94-95.
[4] 张昊.兖州卷柏成分及抗肿瘤活性成分研究[D].长沙:中南大学,2009.
[5] 温扬敏.兖州卷柏的研究进展[J].医药导报,2012,31(3):341-343.
[6] 郭婷婷,解一新,温扬敏.正交实验优化兖州卷柏总黄酮的提取工艺[J].中医药导报,2018,6(5):65-67.
[7] 谢永华,温扬敏,罗彩林,等.兖州卷柏活性物质提取及对人食管癌细胞的抑制作用[J].医药导报,2012,31(9):79-82.
[8] 李朝慧,任本洪,孙雪娇,等.顺铂耐药对食管癌细胞增殖、凋亡、迁移和血管生成的影响[J].中国病理生理杂志,2017,33(1):1-6.
[9] SIEGEL R L,MILLER K D,JEMAL A.Cancer statistics,2018[J].CA:A Cancer Journal for Clinicians,2018,11(3):279-282.
[10] 林东昕,谭文,陆士新,等.中国食管癌分子流行病学研究[J].中华流行病学杂志,2003,24(10):939-943.
[11] 郭晓彤,赫捷.食管癌治疗现状及精准医学时代展望[J].中华肿瘤杂志,2016,38(9):641-645.
[12] 刘雪丽,周学锋,王君瑜,等.中药抗肿瘤作用机制研究进展[J].中国药师,2016,19(6):1158-1162.
[13] 王成双,佐满珍.华蟾素联合顺铂对卵巢癌细胞A2780增殖及凋亡的影响[J].肿瘤学杂志,2017(1):30-34.
[14] 张昊,黄可龙,施树云,等.微波辅助提取兖州卷柏中的总黄酮[J].中南药学,2008,6(6):698-701.
[15] 欧阳高亮,李祺福,洪水根.细胞凋亡与肿瘤的发生发展和治疗[J].国际肿瘤学杂志,2000(5):266-269.
[16] 龙红萍,李福双,杨中保,等.兖州卷柏的肽类化学成分及对低氧/复氧诱导的PC-12细胞损伤的保护作用[J].天然产物研究与开发,2014,26(6):947-951.
[17] 张彩霞,刘阳云.一氧化氮对肿瘤细胞化疗增敏机制研究进展[J].临床与病理杂志,2016,36(8):1224-1228.
[18] 彭金娥,潘敬新.一氧化氮在肿瘤治疗中的多重作用[J].肿瘤,2012,32(1):70-73.
[2] 李慧娜,田少君.油料作物中黄酮类化合物的提取方法研究进展[J].中国油脂,2017(8):111-115.
[3] 林美珍,许振强.兖州卷柏识别及对肝炎的治疗作用[J].海峡药学,2004,16(5):94-95.
[4] 张昊.兖州卷柏成分及抗肿瘤活性成分研究[D].长沙:中南大学,2009.
[5] 温扬敏.兖州卷柏的研究进展[J].医药导报,2012,31(3):341-343.
[6] 郭婷婷,解一新,温扬敏.正交实验优化兖州卷柏总黄酮的提取工艺[J].中医药导报,2018,6(5):65-67.
[7] 谢永华,温扬敏,罗彩林,等.兖州卷柏活性物质提取及对人食管癌细胞的抑制作用[J].医药导报,2012,31(9):79-82.
[8] 李朝慧,任本洪,孙雪娇,等.顺铂耐药对食管癌细胞增殖、凋亡、迁移和血管生成的影响[J].中国病理生理杂志,2017,33(1):1-6.
[9] SIEGEL R L,MILLER K D,JEMAL A.Cancer statistics,2018[J].CA:A Cancer Journal for Clinicians,2018,11(3):279-282.
[10] 林东昕,谭文,陆士新,等.中国食管癌分子流行病学研究[J].中华流行病学杂志,2003,24(10):939-943.
[11] 郭晓彤,赫捷.食管癌治疗现状及精准医学时代展望[J].中华肿瘤杂志,2016,38(9):641-645.
[12] 刘雪丽,周学锋,王君瑜,等.中药抗肿瘤作用机制研究进展[J].中国药师,2016,19(6):1158-1162.
[13] 王成双,佐满珍.华蟾素联合顺铂对卵巢癌细胞A2780增殖及凋亡的影响[J].肿瘤学杂志,2017(1):30-34.
[14] 张昊,黄可龙,施树云,等.微波辅助提取兖州卷柏中的总黄酮[J].中南药学,2008,6(6):698-701.
[15] 欧阳高亮,李祺福,洪水根.细胞凋亡与肿瘤的发生发展和治疗[J].国际肿瘤学杂志,2000(5):266-269.
[16] 龙红萍,李福双,杨中保,等.兖州卷柏的肽类化学成分及对低氧/复氧诱导的PC-12细胞损伤的保护作用[J].天然产物研究与开发,2014,26(6):947-951.
[17] 张彩霞,刘阳云.一氧化氮对肿瘤细胞化疗增敏机制研究进展[J].临床与病理杂志,2016,36(8):1224-1228.
[18] 彭金娥,潘敬新.一氧化氮在肿瘤治疗中的多重作用[J].肿瘤,2012,32(1):70-73.