外引玉米种质对两种穗腐病原镰孢菌抗性鉴定
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摘要
采用双牙签接种技术,2016-2017年连续2年对从美国、加拿大、罗马尼亚、德国、法国、荷兰等国家引进的177份玉米种质资源抗玉米穗腐病原拟轮枝镰孢(F.verticillioides)和禾谷镰孢(F.graminearum)进行了鉴定。结果表明,鉴定的177份玉米种质资源中,对拟轮枝镰孢表现高抗(HR)的资源2份,占总数的1.13%;抗病(R)38份,占总数的21.47%;中抗(MR)75份,占总数的42.37%;感病(S)48份,占总数的27.12%;高感(HS)14份,占总数的7.91%。177份玉米种质资源中,对禾谷镰孢(F.graminearum)表现抗病(R)12份,占鉴定资源总数的6.78%;中抗(MR)66份,占总数的37.29%;感病(S)64份,占总数的36.16%;高感(HS)35份,占总数的19.77%,未发现对禾谷镰孢菌表现高抗的材料。在177份玉米种质资源中,兼抗两种穗腐病原镰孢菌的种质资源有12份。
A total of 177 maize germplasm accessions,which were imported from the countries of USA,Canada,Romania,Germany,France and Netherlands,were evaluated for resistance against ear rot causing agent of Fusarium verticillioides(FV)and Fusarium graminearum(FG)with double toothpick inoculation technique in two calendar years(2016-2017).Out of that,two accessions(1.13% of total tested accessions)were highly resistant(HR)to FV,and 38 accessions(21.47%)were resistant(R).75,48 and 14 accessions showed moderate resistance(MR,42.37%),susceptible(S,27.12%)and highly susceptible(HS,7.91%),respectively.Upon inoculation of FG,12 and 66 accessions were resistant(R,6.78%)and moderately resistant(MR,37.29%),respectively.Meanwhile,64 and 35 accessions were susceptible(S,36.16%)and highly susceptible(HS,19.77%),respectively.No accessions with highly resistant(HR)to FG was found.Attractively,twelve accessions with resistant to both FV and FG were identified,and they might serve as resistance resource used in maize breeding for ear rot resistance.
A total of 177 maize germplasm accessions,which were imported from the countries of USA,Canada,Romania,Germany,France and Netherlands,were evaluated for resistance against ear rot causing agent of Fusarium verticillioides(FV)and Fusarium graminearum(FG)with double toothpick inoculation technique in two calendar years(2016-2017).Out of that,two accessions(1.13% of total tested accessions)were highly resistant(HR)to FV,and 38 accessions(21.47%)were resistant(R).75,48 and 14 accessions showed moderate resistance(MR,42.37%),susceptible(S,27.12%)and highly susceptible(HS,7.91%),respectively.Upon inoculation of FG,12 and 66 accessions were resistant(R,6.78%)and moderately resistant(MR,37.29%),respectively.Meanwhile,64 and 35 accessions were susceptible(S,36.16%)and highly susceptible(HS,19.77%),respectively.No accessions with highly resistant(HR)to FG was found.Attractively,twelve accessions with resistant to both FV and FG were identified,and they might serve as resistance resource used in maize breeding for ear rot resistance.
引文
[1]WHO global strategy for food safety:safer food for better health.Food safety programme-2002,World Healty Organization.Http:www.who.int/fsf.
[2]金红宇,王莹,孙磊,马双成.中药中外源性有害残留物监控的现状与建议.中国药事,2009,23(7):639-642Jin H Y,Wang Y,Sun L,Ma S C.Current situation and suggestions on control of extrinsic harmful residues in Chinese medicinal materials.Chinese Pharmaceutical Affairs,2009,23(7):639-642
[3]Turner N W,Bramhmbhatt H,Szabo-Vezse M,Poma A,Coker R,Piletsky S A.Analytical methods for determination of mycotoxins:An update(2009-2014).Anaytical Chimica Acta,2015,901(2):12-33
[4]黄晓静,王少敏,毛丹,苗水,季申.镰刀菌属真菌毒素的毒性研究进展.食品安全质量检测学报,2017,8(8):3117-3128Huang X J,Wang S M,Mao D,Miao S,Ji S.Research progress on toxicity of Fusarium mycotoxins.Journal of Food Safety and Quality.2017,8(8):3117-3128
[5]Attwater W A,Busch L V.Role of the sap beetle Glischrochilus quadrisignatus in the epidemiology of gibberella corn ear rot.Canadian Journal of Plant Pathology,1983,5(3):158-163
[6]Sutton J C,Baliko W,Liu H J.Fungal colonization and zearalenone acumunation in maize ears injured by birds.Canadian Journal of Plant Science,1980,60:453-461
[7]Hesseltine C W,Bothast R J.Mold development in ears of corn from tasseling to harvest.Mycologia,1977,69(2):328-340
[8]Sutton J C.Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum.Canadian Journal of Plant Pathology,1982,4(2):195-209
[9]Munkvold G P,Carlton W M.Influence of inoculation method on systemic Fusarium moniliforme infection of maize plants grown from infected seeds.Plant Disease,1997,81(2):211-216
[10]Fandohan P,Hell K,Marasas W F O,Wingfield M J.Infection of maize by Fusarium species and contamination with fumonisin in Africa.African Journal of Biotechnology,2003,12(2):570-579
[11]Eller M S,Holland J B,Payne G.Breeding for improved resistance to fumonidin contamination in maize.Toxin Reviews,2008,27(3-4):371-389
[12]Maschietto V,Colombi C,Pirona R,Pea G,Strozzi F,Marocco A,Rossinai L,Lanubile A.QTL mapping and candidate genes for resistance to Fusarium ear rot and fumonisin contamination in maize.BMC Plant Biology,2017,17:20
[13]曲晓丽,徐秀德,董怀玉,王丽娟,姜钰,宋艳春,盖淑军.玉米子粒携带真菌种群多样性分析.玉米科学,2009,17(6):115-117Qu X L,Xu X D,Dong H Y,Wang L J,Jiang Y,Song Y C,Gai S J.Analysis of fungi species diversity on maize kernels.Journal of Maize Sciences,2009,17(6):115-117
[14]秦子惠,任旭,江凯,武小菲,杨知还,王晓鸣.我国玉米穗腐病致病镰孢种群及禾谷镰孢复合种的鉴定.植物保护学报,2014,41(5):589-596Qin Z H,Ren X,Jiang K,Wu X F,Yang Z H,Wang XM.Identification of Fusarium species and F.graminearum species complex causing maize ear rot in China.Acta Phytophylacica Sinica,2014,41(5):589-596
[15]段灿星,朱振东,武小菲,杨知还,王晓鸣.玉米种质资源对六种重要病虫害的抗性鉴定与评价.植物遗传资源学报,2012,13(2):169-174Duan C X,Zhu Z D,Wu X F,Yang Z H,Wang X M.Screening and evaluation of maize germplasm for resistance to five diseases and Asian corn borer.Journal of Plant Genetic Resources,2012,13(2):169-174
[16]邹成佳,崔丽娜,章振羽,张小飞,李荣进,陈耕,李晓.玉米自交系对轮枝镰孢菌穗腐病的抗性评价.西南农业学报,2017,30(6):1346-1349Zou C J,Cui L N,Zhang Z Y,Zhang X F,Li R J,Chen G,Li X.Evaluantion of maize inbred lines for resistance to Fusarium verticilliodes ear rot.Southwest China Journal of Agricultural Sciences,2017,30(6):1346-1349
[17]Lanubile A,Maschietto V,Borrelli V M,Stagnati L,Logrieco A F,Marocco A.Molecular basis of resistance to Fusarium ear rot in maize.Frontiers in Plant Science,2017,8:1774
[18]Scott G E,King S B.Site of action of factors for resistance to Fusarium moniliforme in maize.Plant Disease,1984,68(9):804-806
[19]Hoenish R W,Davis R M.Relationship between kernel pericarp thickness and susceptibility to fusariun ear rot in field corn.Plant Disease,1994,78(5):517
[20]郭成,郭满库,魏宏玉,郭建国.玉米种质资源抗穗腐病鉴定.江西农业学报,2015,27(1):50-52Guo C,Guo M K,Wei H Y,Guo J G.Identification of resistance of maize germplasm resources to ear rot.Acta Agriculturae Jiangxi,2015,27(1):50-52
[21]马秉元,龙书生,李亚玲,李多川.玉米穗粒腐病的病原菌鉴定及致病性测试.植物保护学报,1998,25(4):300-304Ma B Y,Long S S,Li Y L,Li D C.Identification and pathogenicity of the pathogens of corn ear of kernel rot.Journal of Plant Protection,1998,25(4):300-304
[22]Schaafsma A W,Miller J D,Savard M E,Ewing R J.Ear rot development and mycotoxin production in corn in relation to inoculation method,corn hybrid,and species of Fusarium.Canadian Journal of Plant Pathology,1993,15(3):185-192
[23]王丽娟,徐秀德,刘志恒,董怀玉,姜钰,张明会.玉米抗镰刀菌穗腐病接种方法及抗病资源筛选研究.植物遗传资源学报,2007,8(2):145-148Wang L J,Xu X D,Liu Z H,Dong H Y,Jiang Y,Zhang MH.Inoculation technique and screening maize germplasm resistance to Fusarium ear rot.Journal of Plant Genetic Resources,2007,8(2):145-148
[2]金红宇,王莹,孙磊,马双成.中药中外源性有害残留物监控的现状与建议.中国药事,2009,23(7):639-642Jin H Y,Wang Y,Sun L,Ma S C.Current situation and suggestions on control of extrinsic harmful residues in Chinese medicinal materials.Chinese Pharmaceutical Affairs,2009,23(7):639-642
[3]Turner N W,Bramhmbhatt H,Szabo-Vezse M,Poma A,Coker R,Piletsky S A.Analytical methods for determination of mycotoxins:An update(2009-2014).Anaytical Chimica Acta,2015,901(2):12-33
[4]黄晓静,王少敏,毛丹,苗水,季申.镰刀菌属真菌毒素的毒性研究进展.食品安全质量检测学报,2017,8(8):3117-3128Huang X J,Wang S M,Mao D,Miao S,Ji S.Research progress on toxicity of Fusarium mycotoxins.Journal of Food Safety and Quality.2017,8(8):3117-3128
[5]Attwater W A,Busch L V.Role of the sap beetle Glischrochilus quadrisignatus in the epidemiology of gibberella corn ear rot.Canadian Journal of Plant Pathology,1983,5(3):158-163
[6]Sutton J C,Baliko W,Liu H J.Fungal colonization and zearalenone acumunation in maize ears injured by birds.Canadian Journal of Plant Science,1980,60:453-461
[7]Hesseltine C W,Bothast R J.Mold development in ears of corn from tasseling to harvest.Mycologia,1977,69(2):328-340
[8]Sutton J C.Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum.Canadian Journal of Plant Pathology,1982,4(2):195-209
[9]Munkvold G P,Carlton W M.Influence of inoculation method on systemic Fusarium moniliforme infection of maize plants grown from infected seeds.Plant Disease,1997,81(2):211-216
[10]Fandohan P,Hell K,Marasas W F O,Wingfield M J.Infection of maize by Fusarium species and contamination with fumonisin in Africa.African Journal of Biotechnology,2003,12(2):570-579
[11]Eller M S,Holland J B,Payne G.Breeding for improved resistance to fumonidin contamination in maize.Toxin Reviews,2008,27(3-4):371-389
[12]Maschietto V,Colombi C,Pirona R,Pea G,Strozzi F,Marocco A,Rossinai L,Lanubile A.QTL mapping and candidate genes for resistance to Fusarium ear rot and fumonisin contamination in maize.BMC Plant Biology,2017,17:20
[13]曲晓丽,徐秀德,董怀玉,王丽娟,姜钰,宋艳春,盖淑军.玉米子粒携带真菌种群多样性分析.玉米科学,2009,17(6):115-117Qu X L,Xu X D,Dong H Y,Wang L J,Jiang Y,Song Y C,Gai S J.Analysis of fungi species diversity on maize kernels.Journal of Maize Sciences,2009,17(6):115-117
[14]秦子惠,任旭,江凯,武小菲,杨知还,王晓鸣.我国玉米穗腐病致病镰孢种群及禾谷镰孢复合种的鉴定.植物保护学报,2014,41(5):589-596Qin Z H,Ren X,Jiang K,Wu X F,Yang Z H,Wang XM.Identification of Fusarium species and F.graminearum species complex causing maize ear rot in China.Acta Phytophylacica Sinica,2014,41(5):589-596
[15]段灿星,朱振东,武小菲,杨知还,王晓鸣.玉米种质资源对六种重要病虫害的抗性鉴定与评价.植物遗传资源学报,2012,13(2):169-174Duan C X,Zhu Z D,Wu X F,Yang Z H,Wang X M.Screening and evaluation of maize germplasm for resistance to five diseases and Asian corn borer.Journal of Plant Genetic Resources,2012,13(2):169-174
[16]邹成佳,崔丽娜,章振羽,张小飞,李荣进,陈耕,李晓.玉米自交系对轮枝镰孢菌穗腐病的抗性评价.西南农业学报,2017,30(6):1346-1349Zou C J,Cui L N,Zhang Z Y,Zhang X F,Li R J,Chen G,Li X.Evaluantion of maize inbred lines for resistance to Fusarium verticilliodes ear rot.Southwest China Journal of Agricultural Sciences,2017,30(6):1346-1349
[17]Lanubile A,Maschietto V,Borrelli V M,Stagnati L,Logrieco A F,Marocco A.Molecular basis of resistance to Fusarium ear rot in maize.Frontiers in Plant Science,2017,8:1774
[18]Scott G E,King S B.Site of action of factors for resistance to Fusarium moniliforme in maize.Plant Disease,1984,68(9):804-806
[19]Hoenish R W,Davis R M.Relationship between kernel pericarp thickness and susceptibility to fusariun ear rot in field corn.Plant Disease,1994,78(5):517
[20]郭成,郭满库,魏宏玉,郭建国.玉米种质资源抗穗腐病鉴定.江西农业学报,2015,27(1):50-52Guo C,Guo M K,Wei H Y,Guo J G.Identification of resistance of maize germplasm resources to ear rot.Acta Agriculturae Jiangxi,2015,27(1):50-52
[21]马秉元,龙书生,李亚玲,李多川.玉米穗粒腐病的病原菌鉴定及致病性测试.植物保护学报,1998,25(4):300-304Ma B Y,Long S S,Li Y L,Li D C.Identification and pathogenicity of the pathogens of corn ear of kernel rot.Journal of Plant Protection,1998,25(4):300-304
[22]Schaafsma A W,Miller J D,Savard M E,Ewing R J.Ear rot development and mycotoxin production in corn in relation to inoculation method,corn hybrid,and species of Fusarium.Canadian Journal of Plant Pathology,1993,15(3):185-192
[23]王丽娟,徐秀德,刘志恒,董怀玉,姜钰,张明会.玉米抗镰刀菌穗腐病接种方法及抗病资源筛选研究.植物遗传资源学报,2007,8(2):145-148Wang L J,Xu X D,Liu Z H,Dong H Y,Jiang Y,Zhang MH.Inoculation technique and screening maize germplasm resistance to Fusarium ear rot.Journal of Plant Genetic Resources,2007,8(2):145-148